stress but not chemical hypoxia alters the expression of complement regulatory proteins on arpe 19 cells
Báo cáo y học: "Coccidioides posadasii infection alters the expression of pulmonary surfactant proteins (SP)-A and SP-D" pps
... released as a result of FKS vaccination (protective immune response) increase the SP-D expression, but not affect the SP-A expression As reported earlier, the expression of SP-A and SP-D is differentially ... affect the synthesis and secretion of pulmonary surfactant by pulmonary epithelial cells [32,33] In the present study we found slightly increased levels of SP-D in C posadasii protected mice, but not ... phospholipids could be either due to metabolic dysfunction of pulmonary type II epithelial cells during C posadasii infection or due to their utilization in the binding and uptake of C posadasii by...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... lines, with the exception of KG1 myeloid cells A Hypoxia decreases the expression levels of HO-2 protein in YN-1 and K562 cells To confirm the hypoxia- mediated reduction of HO-2 expression, we performed ... acclimatization to hypoxia Results Effects of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of ... Likewise, hypoxia did not influence the expression of HO-2 promoter constructs in YN-1 cells (Fig 7B) In contrast, hypoxia consistently increased the promoter activity of a construct, HRESV40, which contains...
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Investigation of the effects of serum amyloid a on human endothelial cells implications in atherosclerosis
... vasodilation also reflects the alteration of other important functions of the endothelium The impairment of endothelium-dependent relaxations is commonly characterized by the reduced bioavailability of ... prothrombotic phenotype of the endothelium The advanced understanding of the mechanism of atherogenesis suggests that these alterations of endothelial function may play an important role in the development ... anti-LFA-1 treatment of the cells VCAM-1 is a ligand for VLA-4 The firm adhesion of monocytes and T cells to endothelium can be mediated by the interaction of VCAM-1 and VLA-4 on these cells 75 Blocking...
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Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
... Izquierdo JM & Cuezva JM (199 7) Control of the translational efficiency of beta-F1-ATPase mRNA depends on the regulation of a protein that binds the 3¢ untranslated region of the mRNA Mol Cell Biol ... we addressed the question of whether the profile of selected mitochondrial proteins corresponds to the one observed in either fast- or slow-twitch muscles Beforehand, we evaluated whether such differences ... in fast-twitch muscle is affected by PV deficiency Not only the volume and biochemical composition of mitochondria, but also the concentrations of ATP and phosphocreatine, are different between...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... neuronal responses to elevated Ab levels in models of AD [8], suggesting that disruption of the association of these proteins could represent a potential therapeutic strategy for the treatment of ... phosphorylation influences tau content in neuronal DRMs Interactions between tau and Fyn are important for the cellular distribution of these proteins For example, we have shown that the trafficking of tau ... region of tau is critical for the binding of tau to Fyn-SH3 [20] Conversely, using synthetic peptides, we found that Fyn binds strongly to Pro213– Pro 219 of tau, but exhibits little interaction...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... group of cells that were exposed to hypoxic conditions for 6–12 h were re-exposed to normoxic conditions for an additional period of h Collection of cells and fractionation At the end of the culture ... a measure of the hypoxic effect on CytOX gene expression, we measured the steady state CytOX mRNA levels The Northern blot in Fig shows the effect of hypoxia on the levels of mitochondrial genome- ... results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity are secondary to suppression of respiration during hypoxia...
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báo cáo hóa học: " The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice" pptx
... GFAP expression [17,18] The effect of dermal application of CPF on GFAP expression in the hippocampus has not been reported The aim of this study was to determine the expression of GFAP in the ... The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice Journal of ... curve, concentration standards were plotted against logit values Logit of the data was then employed in Microsoft Excel to get the serum concentrations of corticosterone by substitution in the linear...
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Báo cáo sinh học: " Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen" ppsx
... by the consensus Kozak sequence (pCMVcoreKozak) The latter directed the expression of 35-fold more core than the gene devoid of the translation initiation signals, and 16-fold more core than the ... Field Figure Expression of HCV core proteins Expression of HCV core proteins Expression of HCV core protein directed by pCMVcore (A), pCMVcoreKozak (B), pCMVcoreIRES (C) in COS-7 cells 72 h post-transfection ... transcription, apoptosis, lipid metabolism, and the development of immune response [30-33] Extermination of core expressing cells and inhibition of the activity of extracellular core (non-enveloped...
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Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"
... contamination PCR reaction triplicates showed no major differences among them and expression levels of the internal control were similar in the different experiments The expression levels of csp ... parasite development in the sporogonic [3 - 5], exoerythrocytic [6] and erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic ... observations the authors suggested that malaria sporozoites are able to control the protein synthesis machinery in cells of the vertebrate host, which could explain the differences in the levels of expression...
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Chemical neurolysis in the management of muscle spasticity
... only 0.1% of the administered dose remains in the CSF (Ichiyanagi et al., 197 5) The magnitude of the clinical effect of the intrathecal block depends on the concentration of phenol and also on ... infiltration of the proximal third of the muscle The superficial placement of the injection may result in the denervation of the gracilis muscle (which, in addition to thigh adduction, rotates the tibia ... sensory loss in the distribution of the blocked nerve root within 40 seconds of a successful injection Positioning of the patient for the lumbar intrathecal block is critical Once the LP needle...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... 2A,B, overexpression of KLF4 did not influence the expression of SR-BI in control and oxLDL-stimulated cells, but further increased the expression of SR-BI in response to HDL3 stimulation compared ... an important role in the activation of endothelial cells and macrophages, as well as the differentiation and proliferation of VSMCs Overexpression of KLF4 induced expression of multiple anti-inflammatory ... following the basal inhibition of KLF4, the expression of SR-BI was not influenced substantially in the control or oxLDL-stimulated cells Consequently, HDL3 treatment failed to induce expression of SR-BI...
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Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx
... localized on different chromosomes the question arises concerning the mechanisms underlying the orchestrated expression of these enzymes by the oxygen tension The findings that the effect of hypoxia on ... increased the mRNAs of the hydroxylases, but not of procollagen Ia mRNA (Fig 3B) To test for the cell and species specificity of the changes of the enzyme expression in response to hypoxia, we ... oxygen, whilst the expression of PHD-1 is not The physiological meaning of this differential regulation of PHD-gene expression remains to be clarified In spite of the similar regulation of PHD-2, PHD-3...
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Báo cáo khoa học: Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development pdf
... Regulation of Ppp2r5c expression by Nrl J.-W Kim et al precise molecular mechanisms underlying the transcriptional control of PP2A genes and the effects of diverse combinations of PP2A subunits ... in the mouse retina (Fig 4C), as well as the induction of Ppp2r5c transcripts that corresponded to the transcription factor association (Fig 4E) However, prior to the association of Nrl with the ... and expression of Nrl proteins in various cell lines Fig S3 Efficiency of Nrl siRNA on HT22 cells Fig S4 Affinity purification of GST–Nrl proteins This supplementary material can be found in the online...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... the distribution of the amino acid-regulated genes* versus the distribution of the genes spotted onto the microarray** *Percentage of the representation of one GO term among all the amino acid-regulated ... starvation had similar effects on the expression of 12 genes and opposite effects on the regulation of eight genes These results suggest that rapamycin inhibition of TORC1 modifies the expression of ... details of the experiment are given in Table S1 in the regulation of gene expression in response to amino acid starvation However, comparison of the regulatory mechanisms involved in the control of...
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Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt
... system, one is highly ordered and the other relatively disordered [8,18 ,19] Analysis of EPR spectra allowed derivation of the orientation distribution of spin labels, where the z-axis of the molecular ... rotational motion in the environment of the Cys374 site of actin [8] In a recent publication 190 2 the authors argue that in the weak binding state of myosin to actin the heads interact only with one ... orientation of the probe molecules The disorder can arise either from disorder of a local region of the protein or from improper orientation of the filaments X-Ray diffraction experiments led to the conclusion...
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Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
... deficiency Under the experimental conditions used in this study, the knock down of ACS4 expression was performed in isolated cells Under these conditions, cells respond to the acute hormonal stimulus ... 8Br-cAMP and arachidonic acid synthesis [12] On the basis of these results, silencing the expression of ACS4 should also result in inhibition of hormone-induced steroid synthesis siRNA duplexes ... reaching concentrations of progesterone equivalent to maximal steroid synthesis, although it was used at submaximal 8Br-cAMP concentration Once again, incubation of the cells in the presence of 22(R)-OH-cholesterol...
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Báo cáo khoa học: The expression of retinoblastoma and Sp1 is increased by low concentrations of cyclin-dependent kinase inhibitors ppt
... nonspecific annealing of primers to nontarget DNA The lower solution contained the cDNA, the MgCl2, the dNTPs, the [32P]dATP[aP] and one half of the buffer, and the upper solution contained the ... co-transfection experiments DHFR activity increased, depending on the amount of co-transfected p21 (Fig 7E) Discussion We aimed to explore the effects of the inhibition of CDKs on the expression of their ... distribution was determined in K562 to study whether the effect of the three compounds on Rb expression was related to CDK inhibition UCN-01, at 50 nM, caused a change in the distribution of the...
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Báo cáo Y học: The inhibitory region of troponin-I alters the ability of F-actin to interact with different segments of myosin pot
... a concentration of 2.5–4.0 mgÆmL)1 The broad signals of the spectrum of F-actin obtained at these concentrations contributed relatively little to the spectra of the peptides in the presence of ... complex formation The value of the dissociation constant derived, lM (Table 2) was consistent with an analysis of the dependence of the equilibrium plateau signal on the concentration of the TnI inhibitory ... manifest in the narrow line widths of the peptide NMR resonances since linewidth is a monotonic function of the effective correlation time [32] Upon complex formation the bound groups of the peptide...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... attributed to the proliferation of spermatogenic cells around the pubertal stage, and the reduction in the population of Sertoli cells relative to the spermatogenic cells Even though the GR content ... strongly stained with the GR antibody In the case of testes, the immunoreactivity to the anti-GR Ig was strong in Sertoli cells Concerning the intracellular distribution, positive signals to the ... expressed in the Sertoli cells, but was below the detectable levels in spermatogenic cells (Fig 3B) Thus, a high expression of GR in Sertoli cells can be fully confirmed Changes of GR expression in testes...
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Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... significance of the modulation of PSG7, fmfc and BMP-6 expression by T3 also awaits additional studies Up-regulation of 4-1BB and the subsequent activation of the caspases occurred not only in HeLaTR cells ... in other systems where functional TR is expressed As well as treatment of the HeLaTR cells with T3, overexpression of 4-1BB in the HeLaTR cells led to the activation of caspases Activation of ... cells but also in rat FRTL cells that expressed only authentic TR, indicating that the up-regulation of 4-1BB and the subsequent activation of the caspases were not specific to the HeLaTR cells but...
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