... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and ... 5¢-RACE and the two alternate 3¢-ends of exon 12 were deduced by 3¢-RACE, and are delineated by the full-length cDNA clones, GT-cDNA1 and GT-cDNA The two putative polyadenylation sites (ATTAAA) are ... revealed two putative polyadenylation (ATTAAA) signals: one is located 18 bp upstream from the poly (A) of GT-cDNA1 and another is located 11 bp upstream from the poly (A) of GT-cDNA2 (data not...
... the Materials and methods section and analysed by SDS/ Fig Immunodetection of CCT a- subunit in the cytoplasm of E focardii SDS/PAGE of an E focardii cytoplasmic fraction (20 lg, lane 1) andof ... immediately after dilution or after h of incubation at 30 °C b-T1 behaviour was similar to that of b5 tubulin [33] At early times, the bulk of the radioactivity migrates as a broad band with a slower ... from rabbit reticulocyte lysate (2 lg and lg in lanes and 3, respectively) Lanes and were Western blotted and immunostained by an anti-(TCP- 1a) polyclonal Ig Lane was Coomassie stained Molecular...
... density and facilitate better carrier transport Extraction of Series Resistance and Apparent Ideality Factor (n) Special attention has been paid to the analysisof the series resistance (Rs) of the ... and 350°C) The duration of each annealing step was 20 Dark and illuminated (1-sun) I–V data were measured before and after each annealing step The dark currents in Figs 5b and 6a are very different ... Nanoscale Res Lett (2010) 5:1762–1767 Fig Schematic diagram of an all-Si quantumdot super lattice tandem solar cell [2] 1763 a quartz substrate using magnetron co-sputtering of Si and quartz...
... mass and energy balances The heat data obtained from the reaction calorimetry were fitted to a mathematical model based on mass and energy balances for a batch reactor coupled by the reaction rate ... peroxide and catalyst concentration andof the initial temperature of the reactor on the reaction rate and the heat flow behavior were evaluated The hydrogen peroxide solution used was a 30% mass ... equation was used because of the explicit influence of pH and catalyst concentration to the kinetics of the reaction The values of the optimized reaction rate constant, k, and the values of the...
... [Journal of Pharmaceutical and Biomedical Analysis 41 (2006) 274-279], (B) [Journal of Pharmaceutical and Biomedical Analysis 48 (2008) 1361-1367], (C) [Journal of Pharmaceutical and Biomedical Analysis ... The similarities of chromatograms of 10 samples (n = 3) Additional file 3: PDA Chromatograms standard compounds (A) anda XST injection (C), and total ion current chromatograms of standard compounds ... and quantitative analysisand wrote the manuscript PYS and QS assisted HY to identify the characteristic peaks using HPLC-PDA/ESI-MSn All authors read and approved the final version of the manuscript...
... (20-22) A molecular and pathological analysisof evolving pancreatic adenocarcinoma has revealed a characteristic pattern of genetic lesions The challenge now is to understand how these signature ... that the activation of telomerase is a late event (58-60) BRCA2 Inherited BRCA2 mutations are typically associated with familial breast and ovarian cancer syndrome, but also carry a significant ... differentiation and proliferation Other growth factors such IGF-I and PDGF play a role in pancreatic development (1-5) 1.1.2 Cancer of the Pancreas The vast majority of cases of pancreatic cancer are adenocarcinomas...
... the heat flux of the evaporation section, and qv and Tv are the density and temperature of the vapor, respectively hlv (kJ/kg) is the latent heat of vaporization, and Psat and Pa (kPa) are the ... the absorber plate and glass tube inside wall, and hr,g aand hc,g a are radiation heat transfer coefficient and convective heat transfer coefficient between the glass tube outer wall and ambient, ... contains pyrolysis and gasification modules and considers the residual tar and char, which were simulated and validated for biomass air gasification preferably Using that biomass gasification model,...
... GGGGGGGGGGGGGGGH b-Actin F TTGCTACATCGCCCTTGACT b-Actin R TGTGGACGGTTTCCTGAATA F R CCACGAGGATTCCACCAACC CCTTCGTCACCGAGACAAGC reagent following the protocol of the manufacturer, and resuspended in DEPC-treated ... sequence Fe TGATTGGACAGTTYGGTGT Re TACAGYTTGATTTGTTCTT F1 GCCGACAAGGTGACCGTAGT R1 TGTTCTTCTGCTTGCGGTTC R2 TCCTCCCAGTCGTTGGTCAG Oligo-dT adaptor GGCCACGCGACTAGTAC(T)16 Adaptor GGCCACGCGACTAGTAC Oligo-dG ... Laevis (AAV41061), Bemisia tabaci (AAZ17403), Ceratitis capitata (CAJ28987), Salmo salar (AAD30275), Chiromantes haematocheir (AY528900), Locusta migratoria (AY445913), Gallus gallus (P11501), Mus...
... 5.33 In-plane stresses and strains in a laminate 140 Analysisofstressandstrain As the load P is in-plane and symmetrical, the strain distribution across the laminate will be constant, as shown ... linear strains, E E and E, in the vicinity ofa point The state ofstrain at a point ofa material is defined in the two-dimensional case if the direct strains, E and E ~ and the shearing strain, ... plotted along the ordinates and not y An important feature of h s strainanalysis is that we have nof assumed that the strains are elastic; we have taken them to be small, however, with this limitation...
... plane stressand plane strain states The results are presented in Tab (1) and (2) for Al alloy 7050 T7451 and Man-Ten steel, respectively Table Stresses and strains at the notch root – Al alloy ... Glinka were used to verify the validity of the local strain approach and evaluate the strain levels and its relation with the nominal stress applied to a geometrical discontinuity section ofa plate ... concentration factor Kt and compare it with the values determined experimentally and also values presented in the literature For the elastoplastic analysis, a constitutive curve for each material, as...
... Nanoscale Res Lett (2008) 3:534–539 Bias: n+ GaAs contact Si layer GaAs S-GaAs 3ML InAs GaAs n+ GaAs contact Si layer GaAs(001) substrate 24 Fig Typical QDIP structure of GaAs/InAs material ... bottom and top GaAs 1000 nm contact layers with doping density 1018 cm-3 Each layer of InAs is capped by 21 ML spacer GaAs material to form the InAs QDs, and the five layers of GaAs/InAs are called ... height and diameter being 80 and 240 A, respectively The distribution and value ofstrain is mainly determined by two factors The first factor is the shape and volume of QD Based on InAs and GaAs...
... a magnified area of the stress map of the boundary of the QD, calculated using elastic theory [18] from the corresponding strain maps and elastic constants [19] A regularly spaced set of red and ... MDs are marked with arrows (b) Combined image ofa GPA-generated strain map and HTREM image showing the misfit dislocation network The indicated axes are b parallel to h1" i directions anda parallel ... planar view TEM showed that the QDs had a well-defined truncated hexagonal pyramidal shape [9], with an average diameter (d) of 73 ± 12 nm and an average height (h) of 12 ± nm, giving a typical...
... workplace environment and are potential sources ofstressand burnout of cancer care workers in radiotherapy [12] The impetus for the study was to analyze factors for stressand job satisfaction of ... [11] These imbalances with increasing demand of human and material resources on the one hand side anda lack of sufficient financial sources on the other side have produced a negative influence ... one dimensional scale with nine categorical answers (1: very high job satisfaction to 9: total job dissatisfaction) Data analysis The data analysis was carried out with the programme SPSS™ 14...
... Peninsular Malaysia and the states of Sabah and Sarawak in the north of Kalimantan Kuala Lumpur, the national capital, Labuan UNEP/SCS – National Report Malaysia and Putra Jaya form the Federal territories ... meters and with a tidal stream of over knots The West Coast of Peninsular Malaysia has an equatorial climate, with an average annual rainfall of more than 2500mm anda daily temperature that ranges ... the Straits of Malacca and the adjacent waters of the Andaman Sea and the Indian Ocean In the process, an attempt is made to identify, examine, and rank those threats that have transboundary effects...
... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded ... Helgstrand M, Mandava CS, Mulder FA, Liljas A, Sanyal S & Akke M (2007) The ribosomal stalk binds to translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal domain ... displayed as side chains and located in domain III, domain V and the interface of domains G, III and V (B) Mutation sites in domain III that may affect the FA-binding pocket Mutation sites are...
... FITC was measured in the FL1 channel (510–535 nm bandpass filter) Data were recorded and analyzed with flowmax software from Partec Statistical analysisof ELISA experiments Each experiment was repeated ... secondary antibody was quantified by adding the substrate o-phenylenediamine dihydrochloride and measuring the resulting absorbance at 490 nm in a microplate reader SPR analysis SPR analysisof NTD ... Injection began at s and ended at 180 s Table Affinity parameters for NTD–FnBR interactions The parameters were determined by SPR measurements, with immobilized FnBRs of FnBPA and FnBPB as ligands and...
... E GAG A GCC T ACC L TTG A GCC Y TAC E GAG D GAC H CAC D GAC Q CAG A GCA S AGC V GTC S TCC A GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG ... YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP YVDP AA DVG.G.VS.D V 53 Nicotiana MGFVLFSQLPSFLLVSTLL.FLVISHSCRAQNSQ ... CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC K AAG Y TAC I ATT F TTC A GCC T ACA S AGC N AAC V GTC K AAG F TTC S AGC V GTC D GAC L CTC G GGC A GCC...
... position and interacts with the substrate and Glu144 This rotation of Asp142 is accompanied by removal ofa water molecule and adjustments of Tyr10 and Ser93 that seem to compensate Asp140 for ... 22 pKa calculations pKa values for Asp140, Asp142, Glu144 and Asp215 were calculated in several ChiB mutants as described in Materials and methods (Table 3) Calculations were primarily based ... The pKa of Asp140 is much lower than that of Asp142 and Glu144 in all situations where all three residues are present and the one proton shared by Asp140 and Asp142 appears to remain on Asp142...
... in particular countries in sub-Saharan Africa, as well as in the Caribbean and Pacific islands, Central America or Caucasus and Central Asian countries The transaction costs for international ... Poland, Slovak Republic and Turkey), Arab countries (Kuwait, Saudi Arabia and United Arab Emirates) and other donors (Israel and others) China and India also provide aid, but the numbers are ... internationally 45 The members of the DAC are: Australia, Austria, Belgium, Canada, Denmark, Finland, France, Germany, Greece, Italy, Ireland, Japan, Luxembourg, the Netherlands, New Zealand, Norway,...
... 5¢-TT AGCGTATTCTAAAAGATACAAATAATCCTGAATGA TATAAAAAC-3¢ (reverse) The pET151 HP1287 plasmid was amplified using PfuTurbo DNA polymerase and incubated with DpnI to digest the template plasmid Mutated ... protease aprE, at the transcriptional level [6] and, for that reason, TenA is often classified as ‘putative transcriptional regulator’ (http:// au.expasy.org/) A comparative analysisof several fully ... QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) The primers used were: 5¢-TATATCATTCA GGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3 ¢ (forward, the mutagenesis codon underlined) and...