stress and intracellular heat shock protein 72 hsp72
Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... et al (2001) Heat- shock protein 70 antagonizes apoptosis-inducing factor Nat Cell Biol 3, 839–843 24 Jiang B, Wang K, Xiao W, Wang H & Xiao X (2009) ATP-binding domain of heat shock protein 70 ... activation of caspase-3 and caspase-9, and apoptosis in C2C12 myogenic cells [24] Stankiewicz et al [25] found that Hsp70 overexpression stabilizes Mcl-1 protein in heat- shocked cells Hsp70 has ... 110 kDa multifunctional phosphoprotein It is abundantly expressed in both normal and cancerous cells [9], and ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell, as well...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... Structure and properties of the small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat- shock proteins and molecular ... Small heat- shock proteins and their potential role in human disease Curr Opin Struct Biol 10, 52–59 Renkawek K, Stege GJJ & Bosman GJCGM (1999) Dementia, gliosis and expression of the small heat shock ... B (2005) Protein flexibility and rigidity predicted from sequence Proteins 61, 115–126 49 van Montfort R, Slingsby C & Vierling E (2002) Structure and function of the small heat shock protein ⁄...
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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf
... Vierling E (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing a-crystallin domains (Acd proteins) Cell Stress Chaperones 6, 225–237 ... Chaperone activity and homo- and hetero-oligomer formation of bacterial small heat shock proteins J Biol Chem 275, 3721 2– 3721 8 56 Leroux MR, Melki R, Gordon B, Batelier G & Candido EPM (1997) ... Vierling E (2004) Interactions between small heat shock protein subunits 1032 34 35 36 37 38 39 40 41 42 43 44 45 46 and substrate in small heat shock protein- substrate complexes J Biol Chem 279,...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... oxidative stress [12,13] As a major stress- inducible heat shock protein, heat shock protein (HSP) 70 has been shown to protect cells from a number of apoptotic stimuli, including heat shock, tumor ... expression at a level similar to that of the endogenous proteins induced by heat shock This system mimics the antiapoptotic effects of heat shock and is very instrumen2618 tal with respect to our investigation ... activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 and -3 were analyzed Cells over-expressing HSP70 and its deletion...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... small heat shock protein Hsp26 Structure 14, 1197–1204 Bova MP, Huang Q, Ding L & Horwitz J (2002) Subunit exchange, conformational stability, and chaperonelike function of the small heat shock protein ... small heat shock protein Hsp22 (H11 or HspB8) Biochem Biophys Res Commun 315, 796–801 24 Chowdary TK, Raman B, Ramakrishna T & Rao CM (2004) Mammalian Hsp22 is a heat- inducible small heat- shock protein ... numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human HSP22) and R108 (equivalent to K141 of human HSP22) are shown in purple and grey,...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... the role of the serine ⁄ threonine kinase akt and the 90 kDa heat shock protein Mol Pharmacol 65, 407– 415 Song Y, Zweier JL & Xia Y (2001) Heat- shock protein augments neuronal nitric oxide synthase ... alteration in intracellular Ca2+ homeostasis A B Fig Levels of NOS isozymes and HSP90 in brain and aorta of NMS rats treated with HSD and C.I.1 Aliquots of brain soluble material (20 lg protein) and of ... to 6% SDS-PAGE and blotted as described previously nNOS, eNOS and HSP90 were detected with specific mAbs (C) nNOS (open circles) and HSP90 (filled circles) protein detected in (A) and the corresponding...
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Báo cáo khoa học: Characterization of novel sequence motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana potx
... ultrastructure, and stress proteins Cell Tissue Res 301, 433–446 Liang P & MacRae TH (1999) The synthesis of a small heat shock ⁄ a-crystallin protein in Artemia and its relationship to stress tolerance ... small heat shock ⁄ a-crystallin protein, and its relationship to stress resistance in Artemia franciscana embryos J Exp Biol 204, 2339–2350 Willsie JK & Clegg JS (2002) Small heat shock protein ... Giese KC, Buan NR & Vierling E (2004) Interactions between small heat shock protein subunits and substrate in small heat shock protein substrate complexes J Biol Chem 279, 1080–1089 19 Pasta SY,...
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Báo cáo khoa học: "Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 (HSP70) in head and neck vessels" potx
... vessels [14] Heat shock proteins (HSP) HSP are found in all organisms and all cell types They are the most phylogenetically conserved proteins known with respect to both structure and function ... irradiation (59.4 -72 Gy, group 2) and patients treated with neoadjuvant chemoradiation (40 Gy and cisplatin or carboplatin and paclitaxel, group3) Intima of the veins (A, *; + p < 0.05 vs group1 and group ... irradiation (59.4 -72 Gy, group 2) and patients treated with neoadjuvant chemoradiation (40 Gy and cisplatin or carboplatin and paclitaxel, group3) Intima of the veins (A, * p < 0.05 vs group1 and group...
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Báo cáo y học: "Celastrus aculeatus Merr. suppresses the induction and progression of autoimmune arthritis by modulating immune response to heat-shock protein 65" pptx
... involvement of T cells and heat- shock protein Immunol Rev 1990, 118:233-255 28 Celis L, Vandevyver C, Geusens P, Dequeker J, Raus J, Zhang J: Clonal expansion of mycobacterial heat- shock protein- reactive ... against heat shock protein surface epitopes and the induction of IL-10 secretion J Immunol 2002, 168:6463-6469 26 Prakken BJ, Roord S, Ronaghy A, Wauben M, Albani S, van Eden W: Heat shock protein ... celastrol and celaphanol A) [12,15] and the COX pathway (for example, epiafzelechin) [13] In addition, NF-κB activity is inversely related to that of the heat- shock response as the induction of heat- shock...
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báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot
... killer and NK-Like T-cell activation in colorectal carcinoma patients treated with autologous tumor-derived heat shock protein 96 Cancer Res 2005, 65:3942-3949 Srivastava : Roles of heat- shock proteins ... G: Heat shock proteins: to present or not, that is the question Immunology Letters 2004, 9491-2:1-10 Gastpar R, Gehrmann M, Bausero MA, Asea A, Gross C, Schroeder JA, Multhoff G: Heat shock protein ... molecular weight stress proteins Methods 2004, 32:13-20 Segal BH, Wang X-Y, Dennis CG, Youn R, Repasky EA, Manjili MH, Subjeck JR: Heat shock proteins as vaccine adjuvants in infections and cancer...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... infection or heat shock Stress conditions like heat shock, infections, radiation, and exposure to chemicals induce increased levels of heat shock proteins in many cell lines [9] The heat shock proteins ... 24 h or heat shock treated (44°C, 20 min) or heat shock treated and recovered for or h at 37°C (shift assay) Lane – control cells, lane – heat shock treated, lane – heat shock treated and recovered ... RT-PCR1analysis of the heat shock factor blood macrophages RT-PCR analysis of the heat shock factor and heat shock proteins in vaccinia virus infected human blood macrophages HSF1, HSP70, HSP90α and β-actin...
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Báo cáo y học: "A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens" pptx
... presentation and T cell stimulation by dendritic cells Annu Rev Immunol 2002, 20:621-667 Anderson KM, Srivastava PK: Heat, heat shock, heat shock proteins and death: a central link in innate and adaptive ... heat shock proteins with peptides and antigen presenting cells: chaperoning of the innate and adaptive immune responses Annu Rev Immunol 2002, 20:395-425 Srivastava PK, Amato RJ: Heat shock proteins: ... vaccines against cancers and infectious agents Vaccine 2001, 19:2590-2597 Basu S, Srivastava PK: Heat shock proteins: the fountainhead of innate and adaptive immune responses Cell Stress Chaperones...
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Báo cáo khoa học: "Glutamine induces heat-shock protein and protects against Escherichia coli lipopolysaccharide-induced vascular hyporeactivity in rats" pdf
... McMillan DR: Stress (heat shock) proteins: molecular chaperones in cardiovascular biology and disease Circ Res 1998, 83:117-132 Huber SA: Heat- shock protein induction in adriamycin and picornavirus-infected ... EA: The heat- shock proteins Annu Rev Genet 1988, 22:631-677 Ang D, Liberek K, Skowyra D, Zylicz M, Georgopoulos C: Biological role and regulation of the universally conserved heat shock proteins ... arsenite induces heat shock protein- 72 kilodalton expression in the lungs and protects rats against sepsis Crit Care Med 1994, 22:922-929 19 Chu EK, Ribeiro SP, Slutsky AS: Heat stress increases...
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Báo cáo y học: "Heat shock protein 70 expression, keratin phosphorylation and Mallory body formation in hepatocytes from griseofulvin-intoxicated mice" pptx
... nuclear proteins against heatinduced aggregation Exp Cell Res 1994, 214:279-284 Welch WJ, Feramisco JR: Nuclear and nucleolar localization of the 72, 000-dalton heat shock protein in heat- shocked ... Indian childhood cirrhosis and liver steatosis in obesity [17-21] Other proteins, such as ubiquitin and the heat shock protein 70 kDa (HSP70), are also present in MBs and could play a role in their ... intoxication on hepatocytes from C3H and FVB/n mouse strains We monitored the expression of the stress inducible form of the heat shock protein 70 kDa (HSP70i) and the induction of K8/18 phosphorylation...
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Báo cáo sinh học: " A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells" pot
... donors CO and MCJ provided confocal and fluorescence microscopy analyses PFW and APFT participated in the experiments of RT-PCR for mRNA Hsp65 detection AAMC and CLS provided critical input and assistance ... have shown that the DNA vaccine encoding the Mycobacterium leprae 65 kDa heat shock protein (DNA-HSP65) has prophylactic and therapeutic effects in a murine model of TB [17,19,21,22] The prophylactic ... for 40 sec, and extension was carried out at 72 C for 90 sec Messenger RNA for beta-actin was detected by PCR using cDNA and betaactin-specific primers (sense 5' ATGTTTGAGACCTTCAACA-3' and antisense...
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Cloning, expression and characterization of novel helicobacter pylori differentiating antigen heat shock protein 20
... important role in protein stabilization and bacterial survival In this study, a 20kDa protein was identified as a homologue of HslV in the heat shock protein family and termed as heat shock protein 20 ... performance liquid chromatography HRP: horseradish peroxidase HSP: heat shock protein HSP20: heat shock protein 20 HSP60: heat shock protein 60 IAA: iodoacetamide IEF: iso-electric focusing IL: interleukin ... immune response 1.4.2 A new member of heat shock protein in Helicobacter pylori Heat shock protein 20 (HSP20, HP 0515) is a newly identified member of heat shock protein family based on the open reading...
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Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon)
... copes with stress and the function of heat shock proteins Pediatr Res 36:1–6 22 Macario AJL (1995) Heat- shock proteins and molecular chaperones; implications for pathogenesis, diagnostics, and therapeutics ... The biology of heat shock proteins and molecular chaperones Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 533–552 Mestril R, Dillmann WH (1995) Heat shock proteins and protection ... 90-kDa heat- shock protein family Gene 53:235–245 32 Hickey E, Brandon SE, Smale G, Lloyd D, Weber LA (1989) Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein...
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Tài liệu Báo cáo khoa học: Transduced human PEP-1–heat shock protein 27 efficiently protects against brain ischemic insult pptx
... occurrence of oxidative stress in cerebral ischemia [7,8] Heat shock proteins (HSPs) are major stress proteins that are induced in response to a variety of stresses, including oxidative stress [9] HSPs ... CNS glial and neuronal cells compared to heat shock protein 70 and caspase following kainite administration Mol Brain Res 93, 148–163 Mailhos C, Howard MK & Latchman DS (1993) Heat shock protects ... 50 37 25 3 Fig Expression and purification of the PEP-1–HSP27 fusion protein Protein extracts of cells and purified fusion proteins were analyzed by 12% SDS–PAGE (A) and subjected to western blot...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... Actin and small heat shock protein (Eur J Biochem 270) 895 Fig Fluorescence spectra of 140 lM ANS in the absence of added proteins (1) and in the presence of 2.25 lM of intact actin (2) or actin heated ... focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol 91, 963– 972 11 Miron, T., Vancompernolle, K., Vandekerckhove, ... polymerization of actin heated at 43 °C As mentioned earlier, mild heating at 43 °C results in some changes in actin structure, but this treatment does not Ó FEBS 2003 Actin and small heat shock protein (Eur...
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Báo cáo khoa học: The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response pptx
... synergy with heat stress caused a complete inhibition of protein synthesis Thus, at 42 °C the highest tolerable concentrations of BA and HE were 10 and mm, respectively Effects of heat and membrane ... enhanced activation of heat shock genes [20] In the present study, we tested the possible effects of BA and HE on protein stability at non-heatshock temperatures via the heat- induced inactivation ... T 038334) and Agency for Research Fund Management and Research Exploitation (RET OMFB00067 ⁄ 2005 and Bio-00120 ⁄ 2003 KPI) References Pockley GA (2001) Heat shock proteins in health and disease:...
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