step by step in situ hybridization method for localizing gene expression changes in the brain
Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in the AFM surface representation of Fig 2A By comparison with the 3D structure of the ... ion-binding site Therefore, the c ring of A woodii has only 10 membrane-buried negative charges that are essential for binding the ion and also for the rotational mechanism of the ring The c ring ... tartaricus c ring [24], the black rings represent the view from the cytoplasm (open rings in AFM) and the red ones the view from the extracellular side (smaller, closed rings in Fig 3) The inner and...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... conserved in hairpin If one assumes the same number of hairpins in V0 and F0, the rotor of V1V0ATPases has only half the number of ion-binding sites This is seen as the reason for the apparent inability ... woodii The specific ATPase activity of the complete enzyme was in the range 5–9.9 UÆmg)1 protein, depending on the batch This is in the same range as the one determined previously with the incomplete ... filtration and by adding mm ADP The synthesis reaction was started by the addition of mL of valinomycin to induce a DY Furthermore, when only a DpNa was to be applied, the vesicles were incubated in buffer...
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Summary of Ph.D thesis: A study on the embankment caculation method reinforced by geotextile in the construction of highway in Vet Nam
... with a grading of certain materials For the first time in Vietnam , the filtering function , drainage was used in the retaining wall of the path to the bridge on National Road , Tan Thinh , Hanoi ... application form on the basis of elastic theory for the case of plane strain problem In the case of reinforced embankment , to determine the state of stress - deformation of the embankment below the ... that, the research methods completing for calculating results for reliability problems embankment reinforced by geotextile in the construction of roads in Vietnam becomes necessary Within the...
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Báo cáo y học: "Placenta Percreta-Induced Uterine Rupture Diagnosed By Laparoscopy in the First Trimester"
... uterine rupture In general, the area of placenta percreta-induced uterine rupture exhibits more vascularization than the site of previous scar-induced rupture; therefore, uterine rupture caused by ... occur during delivery, the affected site is the lower uterine segment; however, in cases of uterine rupture during the first trimester, the site commonly affected is the fundus, as noted in our ... day The final pathological examination revealed that the chorionic villi had invaded the entire myometrium up to the serosa, confirming the diagnosis of placenta percreta (Fig 3) The length of the...
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Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... responsible for the change in the aggregation kinetics of a-synuclein This will require further experimental proof The absence of MPTP in the aggregated protein points to an indirect, rather than ... 0.177 h)1 for a-synuclein incubated alone, and in the presence of 100 lm MPTP and 200 lm MPTP, respectively Notably, in the presence of the neurotoxin, there was a delay in the lag time for fibrillation ... solubilized by urea Effect of dopamine on MPTP and MPP+ induced changes in kinetics of the aggregation of a-synuclein a-Synuclein was incubated in the presence of 100 lm MPTP, along with 50 lm dopamine...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... chromatin in eggs, binding through the RS region of LBR to linker DNA of chromatin seems to be predominant Then, the binding is supported by the interaction of the NM region and a protein(s) other ... with this method, that the cell cycle-dependent binding of LBR to chromatin is regulated by phosphorylation in the arginine-serine repeatcontaining region (RS-region) by multiple kinases The potential ... binding of LBR to chromatin Fig Identification of chromatin binding domains in the N-terminal region of LBR and analysis of the binding mode Empty columns: four kinds of GST fusion proteins including...
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Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf
... role in the cold shock response [29–32] Taking these findings together, we suggest that the R69L mutant of IF1 allows premature subunit joining by either failing to discriminate between certain ... conformational changes in the 30S subunit [7,25,26] Second, the particular R69L alteration in IF1 results in a general increase in expression of reporter genes [27], while also leading to increased ... terminus was more prominent Thus, there were apparent differences between the mutant plasmids in extended termini, depending on the mutated position in the processing stem Taken together, these...
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Báo cáo khoa học: An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain docx
... showed that the RPE65 mRNA was predominantly expressed in the eye and at lower levels in the brain By contrast, the 13cIMH mRNA was detected at high levels in the brain and at low levels in the eye ... enzyme The 293T cell lysate expressing 13cIMH was incubated with: (A) liposome containing atRE; (B) liposome containing atRE in the presence of mM bypiridine; and (C) liposome containing atRE, in the ... present in the mouse brain (34 pmolÆg)1 brain tissue) [3] and RARs and RXRs also show broad expression in the brain [23] Furthermore, there is evidence that RA signaling is essential for neuronal...
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Báo cáo khoa học: Role of peroxidase inhibition by insulin in the bovine thyroid cell proliferation mechanism pdf
... partition method In the first stage, the hydrophylic upper phase contained the total original activity of the peroxidase inhibitor In the second stage of the purification, 70% of the inhibitory ... combined with the first for further processing The evaporated upper and lower phases were resuspended in water (50% of the original volume) and the inhibitory effects were compared The combined ... same order and with the same volumes of the four mixtures of solvents then used for the elution After seeding the sample, the elution was made in the following order of increasing polarity: (a)...
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Báo cáo sinh học: " Motavizumab, A Neutralizing Anti-Respiratory Syncytial Virus (Rsv) Monoclonal Antibody Significantly Modifies The Local And Systemic Cytokine Responses Induced By Rsv In The Mouse Model" pdf
... below the level of detection of the assay in the non-infected control group, and the objective of the study was to evaluate the effect of motavizumab in the inflammatory response induced by RSV, the ... similar changes in cytokine profiles These studies will provide further understanding of the pathogenesis of RSV infection and should help identifying new targets for developing immunomodulatory therapies ... the same time of the administration of the anti-RSV antibody had no effect on the cytokine profile or other clinical and inflammatory parameters evaluated, therefore those controls were not included...
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báo cáo hóa học: " Methods to recognize work-related cancer in workplaces, the general population, and by experts in the clinic, a Norwegian experience" pptx
... exposure, filling the form and filing the report Informed consent should be obtained prior to filing report Once the report form is filed, it must be up to the labor inspectorate, the insurance scheme ... [29] In this paper we add another weakness by pointing to the lack of defining what doubling of the risk is based on, i.e what is the reference for doubling When no level of risk is defined to ... as basis for doubling, the notion is non-informative, hence a “floating unity” (Figure 1) Therefore, to avoid the problem of referring to doubling of the risk in expert-statements to an insurance...
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Báo cáo hóa học: " Motavizumab, A Neutralizing Anti-Respiratory Syncytial Virus (Rsv) Monoclonal Antibody Significantly Modifies The Local And Systemic Cytokine Responses Induced By Rsv In The Mouse Model" potx
... below the level of detection of the assay in the non-infected control group, and the objective of the study was to evaluate the effect of motavizumab in the inflammatory response induced by RSV, the ... similar changes in cytokine profiles These studies will provide further understanding of the pathogenesis of RSV infection and should help identifying new targets for developing immunomodulatory therapies ... the same time of the administration of the anti-RSV antibody had no effect on the cytokine profile or other clinical and inflammatory parameters evaluated, therefore those controls were not included...
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Báo cáo hóa học: "Research Article A Method for Visualizing Independent Spatio-Temporal Patterns of Brain Activity" potx
... controlled by the user (3) If the user hits the target, the target flashes for 1.5 seconds If the cursor misses the target, the screen is blank for 1.5 seconds (4) The screen goes blank for a 1-second interval ... is logical since the phase of the EEG is not time-locked to the observation intervals Additionally, for the majority of users, two of the remaining four most prominent components for the synchronized ... necessarily independent In order to gain insight regarding the independent patterns generated for each target condition, independent component analysis (ICA) can be performed on the projected features for...
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Báo cáo lâm nghiệp: "Cambioxylophagous fauna of young spruce stands damaged by snow in the Beskids" potx
... gradual At the end of the growing season, 66% of phloem remained live or in the first stage of wilting There is available wood for the invasion of secondary cambiophages in the following growing season ... the degree of wilting up to 90%, however, with the increasing degree of wilting their proportion decreased Larvae and pupae occurred particularly in the phloem in the stage of wilting exceeding ... related to changes induced by damage but also to the microclimate of a lying break in the stand Breaks with the crown dry differently from a standing tree Intensity and velocity of changes in the quality...
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Báo cáo y học: " Protein kinase A-dependent Neuronal Nitric Oxide Synthase Activation Mediates the Enhancement of Baroreflex Response by Adrenomedullin in the Nucleus Tractus Solitarii of Rats" pps
... We further found that the BRR enhancement induced by 8-Br-cAMP was completely blocked by L-NAME (Figure 5B) On the other hand, the BRR-enhancing effect of SIN-1 was not altered by the PKA inhibitor ... activation by ADM and in NTS Since PKG can be activated by nNOS-derived NO [18], we next examined the role of PKG in the BRR enhancement response induced by ADM in NTS Figure 2A shows that co-microinjection ... with the ADM group at 10, 30 or 60 mins B: Representative gels (inset) and quantified data showing changes in the protein level of active form PKG-I in NTS of the rat at 30 after receiving bilateral...
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Báo cáo y học: " Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression" ppsx
... recombinant VZV vaccine strain containing the mumps virus HN gene In this study, we examined whether the HHV-6 MIEp containing intron functioned as a heterologous expression promoter in the VZV ... activity in all cell lines, indicating that the NF-B-binding site in the HHV6MIEp plays an important role in its promoter activity The HHV-6 MIE promoter could drive the expression of foreign gene in ... the VZV vaccine strain Indeed, in the recombinant VZV, HHV-6 MIEp functioned to drive the expression of the DsRed gene, which is a heterologous gene These findings indicate that, like the commercially...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive measured binding assay SI participated in handling ... difference in binding to HeLa in Figure Binding studies for DAF and CAR Binding studies for DAF and CAR A) MOI 0.5 TCID50/cell ofCVB2O, EV7W and CVB5 was incubated with 2.5 × 105 cells in suspension for ... indications of an affinity for DAF that was not affected by temperature, this novel finding needs to be further explored Further investigation using the RT-PCR method to quantify binding of CVB2O and...
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Báo cáo y học: "PPARα downregulates airway inflammation induced by lipopolysaccharide in the mouse" ppt
... were perfused in situ, collected and immersed in 4% paraformaldehyde for 24 h at 4°C Fixed lungs were rinsed in phosphate-buffered saline, dehydrated and embedded in paraffin using standard procedures ... rise in the levels of the CXC chemokines, MIP-2 and KC and of the CC chemokine, MCP-1 Exposure to LPS also induced a marked increase in MMP-2 and MMP-9 activity in BALF, when compared to saline ... inhibits the NFκB pathway, which plays a critical role in LPS signaling as well as in the expression of the chemokines, MIP-2, KC and MCP-1 and of MMP-9 [35] This property could account for the...
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Báo cáo khoa học: "Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland" ppt
... either weak [20] or strong [16] nuclear staining for ER in luminal and glandular epithelia during that same period In the present study, PR staining in uterine tissue was, in general, found in ... present findings and the results from other studies is not clear Figure Immunostaining for ERα and PR in uterine tissue Immunostaining for ERα and PR in uterine tissue A and B: ERα in mare 526 (intact ... found in the glandular epithelium and the myometrium For intact mares, the mare in oestrus (mare 526) showed the strongest staining intensity and the highest proportion of stained cell nuclei in...
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Báo cáo y học: " Modulation of the virus-receptor interaction by mutations in the V5 loop of feline immunodeficiency virus (FIV) following in vivo escape from neutralising antibody" ppsx
... 96) for 30 minutes at 37°C In assessing whether pre-incubation with Fc-CD134 enhanced CD134-independent infection, virus was pre-incubated with Fc-CD134 for 30 minutes prior to plating on the ... that the Env-sFcCD134 interaction itself determines the degree of inhibition and that within the range examined, the level of surface CD134 expression on the target cell does not influence the ... modulates the virus-receptor interaction The data suggest that the viral variants derived in vivo escaped neutralising antibody by the acquisition of mutations in the V5 loop region We therefore generated...
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