stem cells and candidate cells for cardiac cell therapy
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... 1.1 Stem cells 1.1.1 Human pluripotent stem cells 1.1.2 In vitro differentiation potential of human pluripotent and neural stem cells 1.2 Candidate ... human neural stem cells (lt-NES cells) , being precursors of glia und neurons were also suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells Besides...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
... tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially in AC cell ... AK1C3 [19] and CD44 [20] have been proposed to be the markers of cancer stem cells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor ... retinoic acid and the precursor of the storage form vitamin A, respectively CD44 is one of cell- surface glycoproteins which relates to cell- cell interactions including adhesion and migration, and thus...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells in vitro (a) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated ... T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated number of mitomycin c-treated wild-type MSCs for 72 hours and pulsed for ... Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, were...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines ... embryonic-like stem cells, also known as induced pluripotent stem cells (iPSCs), as a potential autologous pluripotent stem cell source that can be generated from a patient’s own skin or blood cells (Takahashi...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... hope of cell replacement as a possible strategy for the CNS is worth considering because stem cells could differentiate into neural cells Neural stem cells would be good candidate for cell replacement ... interactions between the local degenerated cells and the transplanted stem cells 4.2 Stem cell transplantation Stem cells are defined by their capability for self-renewal and multipotent differentiation ... shown to suppress the function of a broad range of immune cells, including T cells, B cells, NK cells and antigen-presenting cells (Fig 4) and thus may have therapeutic effect in autoimmune diseases...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... Adult stem cells 10 1.5.2.1 Bone marrow stem cells 10 1.5.2.1.1 Hematopoietic stem cells 11 1.5.2.1.2 Mesenchymal stem cells 11 1.5.3 Bone marrow stem cell- transplantation ... 1.4 Cardiac myofibrillogenesis 1.5 Novel therapy 1.5.1 Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical ... cardiomyocyte-like cells for cell therapy European Heart Journal 28 Suppl 1:548 Winston Shim, Genevieve Tan, Eugene Sim and Philip Wong (2005) Large Scale Expansion of Cardiomyocyte-like Cells for Cell Therapy...
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Human embryonic stem cells for genotoxicity testing
... Cell viability by MTT assay following exposure of hESC to reduced temperature (4oC and 25oC) for 24h and 48h Table Percentage of aberrant cells in untreated controls and in MMCtreated IMR90 and ... However, established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been ... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure to...
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báo cáo khoa học: " QTLs and candidate genes for desiccation and abscisic acid content in maize kernels" docx
... case, obvious candidates for desiccation QTLs were LEA proteins and aquaporins, whereas obvious candidates for ABA QTLs were key enzymes of the ABA biosynthetic pathway, such as NCED and ZEP, ABA ... pathways Page 14 of 22 and consequently a much larger number of candidate genes Using this approach, several candidates [39] were identified and then validated through molecular and/ or genetic studies ... are potential candidates (Fig 2, Tables and 4) Interestingly, NCED1 (Vp14), NCED2 and NCED6 genes mapped at loci colocating with QTLs for desiccation only (Fig 2, Tables and 4) Therefore, transcript...
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Fabrication, characterization and degradation of PHB and PHBV microspheres for liver cell growth
... many types of cells such as sinusoidal endothelial cells, Kupffer cells, stellate cells, biliary epithelial cells and hepatocytes Sixty percent of the liver is made up of hepatic cells or hepatocytes ... templates for cell adhesion and subsequent tissue formation, since 3D scaffolds have more available surface area for cell attachment and adequate porosity for nutrients and oxygen transport Therefore, ... parenchymal stellate cells [Riccalton-Banks, 2003], and pancreatic islets [Kneser, 1999] Primary cells (autogeneic, allogeneic or xenogeneic) and stem cells were originally used as isolated cell transplantation...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell ... al., 2001) Stem cells as delivery vehicles have been shown to enhance gene delivery efficiency (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), ... quantities of stem cells difficult One of the most-well studied is the adult hematopoietic stem cells, which give rise to all the blood cells Hematopoietic stem cells are in clinical trials for genetic...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem ... stimulated cells were prepared and subjected to SDS ⁄ PAGE as previously described [20] Briefly, stimulated cells were rinsed twice with ice-cold NaCl ⁄ Pi and lysed in ice-cold lysis buffer for 30 Cell ... mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell Res Ther...
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Báo cáo khoa học: Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells ) implication for placental cell fusion pot
... anti-rabbit IgG and DAB A novel band (arrow) of 64 kDa (A1) or 80 kDa (B) was present in whole cell lysates from BFA-treated cells in both control and forskolin-stimulated cells, presumably ... cells (B) Labeling of surface and intracellular antigens after cell permeabilization n ¼ number of cell samples A1 B A2 Fig Detection of partially glycosylated and unglycosylated CD98 after cell ... 12.5 +F -F +F -F +F -F +F Forskolin dimethylsulfoxide increase of CD98 surface expression on intact BeWo cells after BFA treatment for both control and forskolin-incubated cells (Fig 2A) However,...
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Báo cáo y học: "Adult mesenchymal stem cells and cell-based tissue engineering" doc
... ‘bone marrow stem cell , which is often used for either hematopoietic or mesenchymal stem cells, the central nervous system stem cell, the hepatic stem cell, etc.? If many stem cells are found ... hematopoietic stem cells, and stromal cells In a ground-breaking study, Friedenstein et al [30] isolated cells, clonogenic fibroblast precursor cells (CFU-F), from whole bone marrow and showed that ... of stem cells are separate entities or whether they are all descendants of one common stem cell Are the true stem cells circulating and homing to tissues as they are needed? Is the same cell...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and retinoic ... Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath ... Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore A, LeProust EM,...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... 22:138-147 30 Rando TA: Stem cells, ageing and the quest for immortality Nature 2006, 441:1080-1086 31 Gerber I, ap Gwynn I: Influence of cell isolation, cell culture density, and cell nutrition ... functions of the cells, e.g differentiation and proliferation, rely on intact cell- cell interactions and a tight attachment to extracellular matrix components In micromasses, the cells can interact ... cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic Stem Cells Produced Bone Nodules in...
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Báo cáo y học: "Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes" pdf
... significance between NP and annulus fibrosus (AF) cells and NP and articular cartilage (AC) cells (P < 0.05) † statistical significance between AF cells and AC cells (P < 0.05) or AC cells (P < 0.0001), ... between NP and annulus fibrosus (AF) cells and NP and articular cartilage (AC) cells (P < 0.05) † statistical significance between AF cells and AC cells (P < 0.05) compared with normal NP cells (P ... pulposus (NP) and annulus fibrosus (AF) cells and nucleus pulposus (NP) and AC cells (P < 0.05) † statistical significance between AF cells and AC cells (P < 0.05) qRT-PCR validation of cell- specific...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... on to fresh feeder cells for confirmation and further analysis; no cells were frozen This work carries forward a theoretical approach suggested more than a decade ago [12], and demonstrates that ... of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... essential for certain types of experiments, as well as for production of cells for transplantation [27] Despite extensive experience with mouse embryonic fibroblast feeder cell layers for "culture...
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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
... SCS-BTA18-Q and SCS-BTA18-q cells and respective control cells; the functional category, p-values and the number of genes are shown for E coli inoculated SCS-BTA18-Q and SCS-BTA18-q cells and S aureus ... between inoculated cells and control cells at time points for each pathogen challenge and each of the inherited SCS-BTA18QTL alleles; A E coli inoculated cells; B S aureus inoculated cells; C E coli ... was performed at time point zero and control cells and inoculated cells derived from the same cell culture, no differences regarding gene expression between the inoculated and control cells were...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the ... reprogram somatic cells to a state of pluripotency Cell Stem Cell, 2007, 1(1): 39-49 Figure Different methodologies to reprogram Somatic cells can be reprogrammed into pluripotent cells by four primary ... transfer, cell fusion, explantation in cell culture, and by the introduction of defined factors 1.7.1 Cell fusion Cell fusion is a process whereby a cell fuses with another cell to form a hybrid cell...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... alternative to reprograming of terminally committed cells into iPS cells and then deriving NSCs is the direct reprograming of cells into NSCs and neural cells without reverting to a pluripotent state ... Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited ... fresh medium and removed after centrifuging the cells for minutes at 300 g The cells were then replated on CELLstartTM-coated culture plates and expanded in neural expansion medium comprising Neurobasal...
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