stem cell banking for translational stem cell research or stem cell based therapies

Tài liệu INNOVATIONS IN STEM CELL TRANSPLANTATION doc

Ngày tải lên : 18/02/2014, 04:20

... a sort of repair system for the body, they can theoretically divide without limit to replenish other cells as long as the person or animal is still alive When a stem cell divides, each new cell ... aspects of stem cells and cell transplantation Although we are close to finding pathways for stem cell therapies in many medical conditions, scientists need to be careful how they use stem cells ethically ... matched for 8/8 alleles, 252 mismatched for (7/8) allele and 269 mismatched for multiple alleles [22] The overall survival (OS) at years was 55% for 8/8 matched transplant recipients, 40% for those...

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Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt

Ngày tải lên : 20/02/2014, 08:20

... Serving as a sort of repair system for the body, they can theoretically divide without limit in order to replenish other cells for the rest of the person or animal's natural life When a stem cell divides, ... divides, each new cell has the potential to either remain a stem cell, or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell Because ... for our readers to follow the recent developments in the field of stem cells and cell transplantation, via this book, such as authors’ search for the clues to what pathways are used by stem cells...

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Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt

Ngày tải lên : 07/03/2014, 05:20

... as brakes for angiogenesis [33] Similar to tumor suppressors, inhibitors are frequently lost in tumors, creating a permissive environment for expansion Re-expression of such inhibitors in angiogenic ... anterograde transport vesicles The cells were starved for h to initiate secretion, ﬁxed and stained for TSP1 (red) and for COPII component Sec23, a marker of anterograde vesicles, or with COPI component ... and normal tissue Human tumor samples were stained for TSP1 and VEGF, respectively The slides were scored by two independent pathologists (double-blind study) TSP1 Tissue or tumor type Normal...

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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx

Ngày tải lên : 23/03/2014, 07:20

... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell ... 48 Time (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) ... compounds gave similar results to those for selenourea A CL inhibition curves for the four organic selenium compounds are shown in Fig The 50% inhibitory concentration (IC50) of each compound...

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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf

Ngày tải lên : 18/06/2014, 19:20

... staining Staining of phosphorylated AKT (p-AKT Thr308) in melanoma cells exposed to DMSO, vemurafenib, metformin or the combination for 24 hours was performed in permeabilized cells using the phosphoflow ... combination therapy on cell cycle progression Melanoma cells were treated with μM vemurafenib, 10 mM metformin, or the combination for 24 hours Afterwards cell cycle analysis was performed using DAPI ... on the use of metformin, from DM, HS and CN for the care of melanoma cell lines and cell viability studies and from BC-A for the optimization and conduct of cell viability, cell cycle, apoptosis...

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The role of paxillin superfamily members hic 5 and leupaxin in b cell antigen receptor signaling 1

Ngày tải lên : 12/09/2015, 08:16

... 4.22 Y72 is important for LPXN phosphorylation in A20 cells Figure 4.23 Overexpression of various FLAG-tagged LPXN plasmids in A20 cells Figure 4.24 Y72 is important for the inhibitory role of LPXN ... bacteria for phagocytosis and sensitizing infected or tumour cells for antibody-mediated cytotoxicity by killer cells (Parkin and Cohen, 2001) Our current project focuses on B cell receptor signaling ... Heng, thanks for their constant support and having the faith in me to complete my PhD Finally, special thanks to a special friend, Jackson Chiam, for his love and support I thank God for without...

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The role of paxillin superfamily members hic 5 and leupaxin in b cell antigen receptor signaling 2

Ngày tải lên : 12/09/2015, 08:16

... FIGURE Tyr72 of LPXN is important for its inhibition of IL-2 production in BCR-stimulated A20 B cells A, Tyr72 is important for LPXN phosphorylation in A20 cells A20 cells were transiently transfected ... fixed for 20 on ice with 4% paraformaldehyde in PBS After permeabilization at room temperature for 10 with 0.2% saponin and 0.03 M sucrose in 1% BSA-containing PBS, cells were washed twice before ... ECL detection system (Amersham Biosciences) For immunoprecipitations, cell lysates were precleared with protein A/G Plus-agarose (Santa Cruz Biotechnology, Inc.) for h at °C For immunoprecipitation...

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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

Ngày tải lên : 14/09/2015, 13:38

... other model systems and sophisticated post-genomic analyses 1.4.2 Medaka as unique model for stem cell research Medaka is an excellent complementary system to human and mouse for stem cell biology ... culture requirements Feeder -cell dependent LIF dependent FGF4 Growth characteristics Ability to form trophoblast Teratoma formation in vivo EB formation Ability to form germ cells in vitro Mouse Human ... with shaking for 16-24 h The probe was visualized by specifically immunostaining for digoxigenin The slides were then washed three times for 30 in 50% formamide, × SSC at 65°C and once for 30 in...

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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt

Ngày tải lên : 20/06/2014, 07:20

... stem/ progenitor cells [11] and is important in the future application of MUC 1based therapies for complete cancer eradication The aforementioned observations suggest that MUC4 may have an important ... contrast, no colony formation was observed in vector-transfected SKOV3 cells (Figure 3) We further isolated these colonies for the stem/ progenitor marker analysis Expression of cancer stem cell and self-renewal ... population of stem/ progenitor cells in the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells...

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báo cáo hóa học: " Function, Adjustment, Quality of Life and Symptoms (FAQS) in Allogeneic Hematopoietic Stem Cell " potx

Ngày tải lên : 20/06/2014, 15:20

... self-management This research will also provide valuable information needed for the development and testing of interventions for patients at high risk for poor health outcomes during the survivorship phase ... experience of transplant survivors, generate testable hypotheses for future research, direct priorities in the development, and testing of supportive care interventions for survivors of allogeneic transplant ... understudied in prior stem cell transplant survivorship research, including Spanish speakers, and individuals undergoing reduced intensity transplantation or receiving treatment for disease relapse...

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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Ngày tải lên : 10/08/2014, 05:21

... Authors’ contributions CCT performed research and analyzed data, CLC designed research, performed research and wrote the paper, HCL contributed vital new reagents or analytical tools, YTL performed ... serum free conditions for 14 days (Figure 3B) For co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated ... Before hybridization to the array, the hybridization mixtures were denatured at 95°C for and incubated at 37°C for 30 To remove any precipitate, the mixture was centrifuged at ≥ 14,000 × g for...

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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" doc

Ngày tải lên : 12/08/2014, 04:21

... relapsed and two cycles of DexaBEAM with stem cell harvest were applied We performed high-dose chemotherapy according to the BEAM protocol On day twelve after stem cell reinfusion he developed mental ... have to be performed to examine the frequency of clinically relevant HHV-6 infections in these patient cohorts Consent statement Written informed consent was obtained from the patient for publication ... of infections after autologous stem cell transplantation or intensive chemotherapeutic treatment, HHV-6 detection should be included into the diagnostic work-up for these patients and longitudinal...

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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" ppsx

Ngày tải lên : 12/08/2014, 04:22

... relapsed and two cycles of DexaBEAM with stem cell harvest were applied We performed high-dose chemotherapy according to the BEAM protocol On day twelve after stem cell reinfusion he developed mental ... have to be performed to examine the frequency of clinically relevant HHV-6 infections in these patient cohorts Consent statement Written informed consent was obtained from the patient for publication ... of infections after autologous stem cell transplantation or intensive chemotherapeutic treatment, HHV-6 detection should be included into the diagnostic work-up for these patients and longitudinal...

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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)

Ngày tải lên : 09/09/2015, 11:21

... filtration Transforming growth factor-alpha Tumor necrosis factor-alpha TNF receptor Tocopherols xx UDCA ULN VEGF Δψm Ursodesoxycholic Acid Upper limit of normal Vascular endothelial growth factor Mitochondrial ... hepatotoxicity mediated injury mechanisms provide key indicators for injury monitoring and also for devising management strategies Therefore, these will be discussed in detail in the following section ... like to thank my PhD supervisor, Dr Ho Han Kiat for his advice and sharing which encouraged me to move on to this journey of research Thank you for giving me the opportunity to join the wonderful...

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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system

Ngày tải lên : 09/09/2015, 11:29

... conditions that are conducive for normal stem cell self-renewal and differentiation This would show if the stem cells from diseased tissue retain their abnormal behavior Therefore, this thesis focuses ... he has for his research was contagious and motivational for me I am also thankful for the excellent example he has provided as a successful scientist and professor The members of the ENT research ... vitro culture system of human nasal epithelial stem or progenitor cells (hNESPCs) 91 4.1 Results 91 4.1.1 Stem cell marker screening in nasal epithelium 91 4.1.2 Formulation...

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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Ngày tải lên : 09/09/2015, 17:54

... neural tube forms, which will eventually differentiate into the spinal cord and the brain, forming the central nervous system Following neural tube closure, patterning is important for determining ... to differentiate in the co-culture system for days 3.5.2 Isolation and expansion of neural progenitor cells (NPCs) In order to enrich for the neural precursor population, the differentiating hESC ... subculture of ReN-VM cells was performed every to days, by dissociating into single cells following incubation with Accutase for minutes, collecting cells by centrifugation at 1000 rpm for minutes and...