... Nakano K, Okada Y, Saito K, Tanikawa R, Sawamukai N, Sasaguri Y, Kohro T, Wada Y, Kodama T, Tanaka Y: Rheumatoid synovial endothelial cells produce macrophage colony-stimulating factor leading ... aberrantly activated: an increase in macrophage infiltration Page of 15 of the synovium promotes inflammation via the production of TNF and other proinflammatory cytokines, and an increase in osteoclast ... clinical improvement in three refractory cases Ann Med 2003, 35:362-367 10 Koyama K, Hatsushika K, Ando T, Sakuma M, Wako M, Kato R, Haro H, Sugiyama H, Hamada Y, Ogawa H, Nakao A: Imatinib mesylate...
... pC4Meth-100TorA/P2 was constructed by PCR, using pTorA/P2 [17] as a template and the primers RRTorA-SacI-fw (5¢-GCGCGGAGCTCAAGAAGGA AGAAAAATAATGAAC-3¢, SacI site underlined) and TorA/Lep2-BamHI-rv (5¢-GCATGGATCCCGCGCGC ... SufIHAXbaI+ClaI-rv (5¢-ACTGATCGATCTAGATTACGCAT AGTCAGGAACATCGTATGGGTAGCCGCCTGGCG GTACCGGATTGACCAAC-3¢, ClaI site underlined, XbaI site in italics, HA-epitope sequence in boldface) The resulting fragments ... and resuspended in M9-medium containing a cysteine- and methionine-free amino acid mix After recovery for 15 (pBAD18-SufIHA harboring strains) or 90 (pJH42 harboring strains) at the appropriate...
... 5’-GTGCATCATCGCTGTTCATACA TNF Forward: 5’-GTGATCGGTCCCAACAAG-3’ Results 71 X66539 Reverse: 5’-AGGGTCTGGGCCATGGAA-3’ b actin Forward: 5’-AGGCCAACCGTGAAAAGATG-3’ 101 NM_031144 Reverse: 5’-ACCAGAGGCATACAGGGACAA-3’ ... radiotherapy Staining was variable between the basal and apical regions of the crypts and did not significantly change of the course of radiotherapy (Data not shown) IL-6 IL-6 staining was weak-moderate ... received no radiotherapy There was an increase in protein expression of TNF after radiotherapy, particularly after 22.5 Gy and 30 Gy as indicated by the arrow, although the staining was not considered...
... the charge attraction between DNA phosphates and the amino groups of polyamines As the amino groups of polyamines are already engaged in ionic bonds with the phosphates of NAPs, secondary amino ... forces playa greater roleintheA Z transition than they inthe B–Z transition, because the difference inthe linear charges density is greater between theA and the Z forms than between the B and ... Saminathan M, Thomas T, Shirahata A, Pillai KS & Thomas TJ (2002) Polyamine structural effects on the induction and stabilization of liquid crystalline DNA, potential applications to DNA packaging,...
... this time the valency hybrid tetramers As a result, the b chain was found to acquire a noticeable resistance against the acidic autoxidation ina manner of contacting with thea chain, no matter ... state the partner a chains may take, the oxy-form or the ferric met-form Unlike separated b chains, the spontaneous formation of hemichrome was at variance with separated a chains inthe pH range ... isolated a chain In contrast to this, the heme pocket of the b chain still obstructs easy access of a water molecule as well as a proton, so that the b chains can keep a constant resistance against the...
... acetylsalicylic acid (ASA) was added to the anticoagulant [17] The final concentration of ASA inthe blood was mM Platelet aggregation and ATP-secretion in blood Whole blood platelet aggregation was ... wall shear rate of 500s-1 Then hirudin-anticoagulated blood containing mepacrine (10 μM) in order to visualize platelets was added to the inlet well, and chambers were perfused for 10 at a wall ... By using NSC23766, our group recently unraveled a Ca2+ -dependent pathway regulating secretion in thrombin-stimulated human platelets linking Rac1 activation to actin dynamics: Calcineurin®Rac1...
... aligned using Clustal X [12] To gain an initial insight into possible recombination events, each of the eight data sets was analyzed respectively using the 3SEQ [13], the Chimaera [14], and the ... derived by plaque purification Furthermore, the same laboratory was the source for all four recombinants and the one putative parental strain As suggested in influenza A virus [9], further work ... shifts for each of the recombinants have strong bootstrap support (data not shown) However, large influenza viral genes inthe databases may actually represent assembled artifactual contigs from...
... interpersonal communication Therole of international health and development organizations in promoting, supporting and advocating the use of well- planned mass media campaigns can also make a ... study has aimed to examine the association between AIDS awareness and a set of independent variables The set of independent variables are women educational attainment, current engagement in an income ... monitoring and evaluation regarding AIDS awareness In this regards a few national and international researchers have made attempts to understand the reasons and come up with some explanations...
... in East Asia; (iii) Bangladesh, India, and Sri Lanka in South Asia; (iv) Cambodia, Indonesia, Malaysia, the Philippines, Thailand, and Viet Nam in Southeast Asia; and (v) Papua New Guinea and ... Southeast Asia and two countries from each of Central Asia, East Asia, South Asia, and the Pacific) Bangladesh and Myanmar, only data on manufacturing are available; with Myanmar only providing ... 1.2: Data Compilation Flow South Asia Central and West Asia BAN, IND, and SRI KAZ, KYR,* and TAJ* Southeast Asia East Asia Pacific Asia CAM, INO, LAO*, MAL, MYA*, PHI, THA, and VIE PRC, KOR, and...
... conducted a review of the sustainability of organic grain production on the Canadian Prairies, including many of the Canadian studies discussed in detail below Notably, the authors conclude that management ... Dairying in Maritime Canada; M.Sc Thesis; NSAC and Dalhousie University: Halifax, NS, Canada, 2001 57 Main, M.H.; Lynch, D.; Martin, R.C.; Fredeen, A Sustainability profiles of Canadian dairy farms ... Sustainability 2011, 332 Peters et al [52] in Australia using an LCA analyses considered three scenarios; (1) a sheep meat supply chain in Western Australia, (2) a beef supply chain in Victoria, Australia...
... that the PX domain of p47phox binds intramolecularly to the SH3 domain inthe same protein, and that this intramolecular interaction suppresses the lipid-binding activity of the PX domain inthe ... domains, suggesting that the PX domain could bind to an SH3 domain of FAD49 To test whether the PX domain could interact with an SH3 domain in FAD49, we performed in vitro binding assays using ... (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions The total RNA was converted to single-stranded cDNA using a random primer and ReverTra Ace (Toyobo, Osaka, Japan) The...
... structural data for the PA–substrate interactions inthe transition state are missing and only a GRID computational modelling approach to the tetrahedral intermediate in PA presents some indications ... substrates The data for the PA catalysed hydrolysis of PhAc-Asp and PhAc-Glu [13] and our data for PhAc-pAB, PhAc-mAB and PhAcoAB (Table 2) imply that the COOH group has to be positioned as in NIPAB ... protein chain, acting as a flap, first opening and then closing the groove [45] The catalytic reaction presumably occurs when the ionized carboxyl group of PG approaches the guanidine side chain...
... (Kirkegaard and Perry, Gaithersburgh, MD, USA) per wellThe plate was incubated inthe dark for 20 and the reaction stopped by the addition of 50 lL of 0.5 M sulfuric acid The plate was read on a Labsystems ... LPCAT activity significantly inhibits LPS-induced TNF -a and IL-6 production, strongly suggesting that LPCAT plays an important rolein mediating the signaling pathways for LPS-activation of these ... confirms that IFN-c can increase the activity of an enzyme, LPCAT, that participates inthe rapid turnover of PtdCho Lysophospholipid acyltransferases maintain membrane lipid composition and the asymmetrical...
... and pA12L-reverse: 5'-CAGGATCCTTAATACATTCCCATATCCA GACAAC; p233-forward: 5'ATGGCGGATAAAAAAAATTTAGCC and A1 2L-reverse: 5'TTA ATACATTCCCATATCCAGACAAAATTCG In order to construct A1 2L with abrogated ... 55–57 (underlined), 5'CTTAATTCTCAAACAGATGTGACTATCGACATCTGTGATACAAAATCAAAGAGTTCA-3' The AG /A site-mutated A1 2L was inserted in pRB21 vector References For transfection of the plasmids into T-REx ... similar to the processing of the other VV core proteins in that the cleavage is sensitive to rifampicin, takes place at the conserved recognition motif, Ala-Gly-Ala (AG /A) , and is associated...
... and pA12L-reverse: 5'-CAGGATCCTTAATACATTCCCATATCCA GACAAC; p233-forward: 5'ATGGCGGATAAAAAAAATTTAGCC and A1 2L-reverse: 5'TTA ATACATTCCCATATCCAGACAAAATTCG In order to construct A1 2L with abrogated ... 55–57 (underlined), 5'CTTAATTCTCAAACAGATGTGACTATCGACATCTGTGATACAAAATCAAAGAGTTCA-3' The AG /A site-mutated A1 2L was inserted in pRB21 vector References For transfection of the plasmids into T-REx ... similar to the processing of the other VV core proteins in that the cleavage is sensitive to rifampicin, takes place at the conserved recognition motif, Ala-Gly-Ala (AG /A) , and is associated...
... the instrument involves qualitative and quantitative approaches The qualitative part consisted of obtaining information relevant to the study behaviour (i.e., adopting the new nursing role) according ... participants to determine their intention regarding the hypothetic role, since none of them has played this precise roleinthe past There is also a potential influence of the social desirability bias ... Group on Behaviour and Health, Faculty of Nursing, Laval University, Québec, Canada 2Canada Research Chair on Behaviour and Health, Laval University, Québec, Canada 3Faculty of Nursing, Laval University,...
... altered sulphate compartmentalization BMC Plant Biol 2010, 10:78 Tomatsu H, Takano J, Takahashi H, Watanabe-Takahashi A, Shibagaki N, Fujiwara T: An Arabidopsis thaliana high-affinity molybdate ... of Arabidopsis mutants for the group sulfate transporters indicates arolein sulfate translocation within developing seeds Plant Physiol 2010, 154:913-926 Kataoka T, Watanabe-Takahashi A, Hayashi ... Takahashi H, Watanabe-Takahashi A, Smith FW, Blake-Kalff M, Hawkesford MJ, Saito K: The roles of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis...
... demonstrate an increased translation of these transcripts and validate the array data indicating no change or a slight decrease in LTBP1, SYNE-1 and MMP3 transcript levels inthe total RNA compartment and ... transport Proc Natl Acad Sci USA 2001, 98:5306-5311 Tanaka T, Yamamoto J, Iwasaki S, Asaba H, Hamura H, Ikeda Y, Watanabe M, Magoori K, Ioka RX, Tachibana K, Watanabe Y, Uchiyama Y, Sumi K, Iguchi ... Hamakubo T, Naito M, Auwerx J, Yanagisawa M, Kodama T, Sakai J: Activation of peroxisome proliferator-activated receptor delta induces fatty acid betaoxidation in skeletal muscle and attenuates...