staining double immunofluorescence staining and tunel assay
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... adiponectin and the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance ... resistance, type diabetes, and cardiovascular disease Diabetes 2007; 5: 1198-209 Hu XB, Zhang YJ, Zhang HT, et al Cloning and expression of adiponectin and its globular domain, and measurement of the ... to their environment before the experiment And then, the mice were randomly divided into three groups: normal control group, diabetic control group, and diabetic treated group Each group included...
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the effect of treaties on foreign direct investment: bilateral investment treaties, double taxation treaties, and investment flows doc
... Treaties and Their Grand Bargain 109 jeswald w salacuse and nicholas p sullivan Bilateral Investment Treaties and Foreign Direct Investment: A Political Analysis 171 tim büthe and ... Tax and Investment Treaties on Bilateral FDI Flows to Transition Economies 687 tom coupé, irina orlova and alexandre skiba Selected Bibliography on Bilateral Investment Treaties and Double ... domestic and international tax issues, and is the author of International Tax as International Law: U.S Tax Law and the International Tax Regime (2007) and U.S International Taxation: Cases and Materials...
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Báo cáo khoa học: Investigation of leucine-rich repeat kinase 2 Enzymological properties and novel assays pptx
... implicated in PD, and understanding its properties and functions will aid in our understanding of disease pathology and progression Experimental procedures Chemicals and reagents All compounds and proteins ... Coomassie Blue staining demonstrated that they are all of similar, high, purity (> 85%) and have similar banding patterns of minor contaminants (Fig 1A) Western blotting showed that the major band at ... characterization of LRRK2 and FEBS Journal 276 (2009) 466–478 ª 2008 The Authors Journal compilation ª 2008 FEBS V S Anand et al LRRK2 characterization provides a protein and assay system that can...
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Báo cáo khoa học: Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay doc
... Neb-ODAIF(1–7) Fig ACE competition assay IPEP (50 lM, 25 lM, 10 lM and lM); IPEPYVWD (25 lM, 10 lM and lM); IP (10 lM and lM) were incubated with lM p-[32]benzoylglycylglycylglycine and fly hemolymph Fig Digestion ... °C and were terminated by addition of 1% trifluoroacetic acid (v/v) The internal standard FMRF-amide was added and samples were centrifuged at 20 000 g for 10 at °C Supernatants were collected and ... by capillary zonal electrophoresis [24] Competition assay The ACE competition assay is based on the ACE activity assay using a simple radio assay for angiotensin-converting enzyme [14,15,25] Briefly,...
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Báo cáo " Isomeranzin against Herpes simplex virus in vitro from Clausena heptaphylla (Roxb.) W. & ARN.: Isolation, structure and biological assay " potx
... ml of saline (for virus control and cell control) and given ml 2x MEM The cells were incubated for 72 hours and observed for cytopathic effects from the virus and cytotoxic effects from the samples ... dimethyl sulfoxide (DMSO), ethyl acetate or ethanol These solutions were assayed against HSV-1 and HSV-2 using a cell protection assay Immortalized African Green Monkey Kidney (Vero) cells (ATCC) were ... Dr Tran Ngoc Ninh, Vietnamese Academy of Science and Technology Extraction and isolation Leaves of Clausena heptaphylla were dried at 40 ÷ 500C and powdered The powder (500 g) was extracted with...
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Focal pointa proven system to simplify your life double your productivity and achieve all your goals
... accomplishment on one hand and far more balance and simplicity on the other hand The good news is that thousands of successful, happy men and women have discovered methods, techniques, and strategies ... goes hand in hand with high productivity, high performance, and high output Result orientation is based on your asking and answering the following four questions, over and over, every minute and ... will become more and more productive You will accomplish more and more Your productivity, your performance, your output, and eventually your pay will increase and eventually double You will begin...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is poorly sensitive and the in vitro ... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is poorly sensitive and the in vitro ... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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Báo cáo toán học: "Comultiplication rules for the double Schur functions and Cauchy identities" docx
... symmetric group and dimensions of skew diagrams; cf [22] I am grateful to Grigori Olshanski for valuable remarks and discussions 2.1 Double and supersymmetric Schur functions Definitions and preliminaries ... in λ and such that λ and µ have the same number of boxes on the main diagonal; and (−1)n(λ/µ) ϕλ/µ (a) sλ (x/y ||a), sµ (x/y) = λ summed over diagrams λ which contain µ and such that λ and µ ... Kλµ and λ χλ (a) = χµ (a) = χλ µ µ (5.15) λ Moreover, Kλµ (a) and χµ (a) are zero unless |λ| |µ|, while Kλµ (a) and χλ (a) are zero µ unless |λ| |µ| Using the duality properties of the double and...
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Báo cáo toán học: "Double-critical graphs and complete minors" pps
... both r and t must have at least one non-neighbour in B(xs), and, since r and t are adjacent, it follows that r and t must have distinct non-neighbours, say q and u, in B(xs) Now, q, r, s, t and ... Proposition 4.1 Let G be a graph decomposable into G1 and G2 Then G is doublecritical if and only if G1 and G2 are both double- critical Proof Let G be double- critical Then χ(G) = χ(G1 ) + χ(G2 ) Moreover, ... subset A and B such that the induced graphs G[A] and G[B] are edge-empty and complete, respectively, and, thus, we have a contradiction by Proposition 5.1 Suppose |S| 4, and let H1 and H2 denote...
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Báo cáo toán học: "Double-dimer pairings and skew Young diagrams" pps
... On the left are the chains in a random double- dimer configuration in a boxshaped region with many nodes all along its boundary The loops and doubled edges of the double- dimer configuration are not ... of these ideas, consider the double- dimer model on εZ × εN, and its scaling limit on R × R+ , and suppose that there are 2n boundary nodes which alternate in color and are at locations x1 , ... the binary relation ←, and we will use it in § when we study the double- dimer model Lemma 1.1 Let P1 , P2 be BPEs of equal length, S1 the confining subset associated to P1 () and π2 the pairing associated...
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Báo cáo y học: "Duodenal enteroglucagonoma revealed by differential comparison of serum and tissue glucagon reactivity with Siemens'''' Double Glucagon Antibody and DakoCytomation''''s Polyclonal Rabbit Anti-Human Glucagon: a case report" ppsx
... aspiration, disseminated candidiasis with candidal sepsis, wound infection, disseminated varicella, atrial fibrillation, acute renal failure and a subhepatic intra-abdominal candidal abscess Discharge ... this article as: Vanderlan et al., Duodenal enteroglucagonoma revealed by differential comparison of serum and tissue glucagon reactivity with Siemens' Double Glucagon Antibody and DakoCytomation's ... measured using Siemens' Double Glucagon Antibody and found to be depressed (37 ng/L; normal 40-130 ng/L) The serum serotonin level (< 25 ng/ mL) was low (normal 90-195 ng/L) and the vasoactive intestinal...
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Layered Double Hydroxides: Present and Future :Lớp đôi hydroxit: Hiện tại và tương lai
... Structure and Characterisation Layered Double Hydroxides: Synthesis and Post-Synthesis Modification A de Roy, C Forano and J P Besse Crystal Structure and X-ray Identification of Layered Double ... Infrared and Raman Spectroscopic Studies of Layered Double Hydroxides (LDHs) J T Kloprogge and R L Frost Solid-State NMR and EPR Studies of Hydrotalcities João Rocha Investigating Layered Double ... (Layered Double Hydroxides) in Catalysis Francesco Basile and Angelo Vaccari Hydrogenation Catalysis by Mixed Oxides Prepared from LDHs A Monzón, E Romeo and A J Marchi Layered Double Hydroxides and...
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Báo cáo khoa học: "Biological in-vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland" ppsx
... conditions or different kinds of ionizing irradiation [18] Page of DLH 15 10 Proband Proband Proband Proband Proband Regression 3D IMRT 0,0 0,5 1,0 1,5 2,0 dose (Gy) 20 40 60 80 100 120 140 dose ... study and provided informatics and support with statistics for data analysis KH participated importantly in the conception and design and helped to draft the manuscript All authors read and approved ... formation and repair of DNA double- strand breaks after computed tomography examinations Proc Natl Acad Sci USA 2005, 102:8984-8989 Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM: DNA doublestranded...
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Báo cáo khoa học: "Lymphangiosis carcinomatosa in squamous cell carcinomas of larynx and hypopharynx – value of conventional evaluation and additional immunohistochemical staining of D2-40" ppsx
... diameter was investigated For standard staining and immunohistochemistry serially prepared sections were examined In large tumors, especially areas of the lateral and deep tumor borders (front ... correlated with nodal involvement, stage and size of the tumor, and survival Sensitivity and specificity were calculated Data were processed by Microsoft Excel and SPSS for descriptive statistic values, ... lymphatic vessel invasion in SCC of larynx and hypopharynx with special respect to predict the risk of nodal metastases and individual prognosis Materials and methods 119 cases (between 1996 up to...
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Báo cáo y học: "Atypical clinical presentation of a subset of patients with anti-RNA polymerase III - nonscleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining" potx
... TTW and MS carried out the immunoassays AC and MS designed the study MS performed the statistical analysis MRB, ESS and WHR enrolled patients for the study and maintained the database AC, MS and ... swelling and pain in her hands and sclerodermatous skin changes to her forearm She visited UFCAD and had a diagnosis of diffuse SSc RP developed two years later Two patients (cases and 2), who ... Chan, and the UF GCRC staff and Matthew Paulus for assistance with clinical data collection This study was supported by NIH grant R01-AR40391 and M01R00082 from the U.S Public Health Service and...
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Báo cáo y học: "omparative Efficacy and Tolerability of 5-Loxin® and Aflapin® Against Osteoarthritis of the Knee: A Double Blind, Randomized, Placebo Controlled Clinical Study"
... as per the standard AMES bacterial reverse mutation assay, chromosomal aberration test in Chinese hamster cells and mouse peripheral blood micronucleus assay [18-21] The efficacy and tolerability ... efficacy of 5-Loxin® and Aflapin® in comparison with the placebo group in terms of improvement in pain and physical function scores at baseline and on days 7, 30, 60 and 90 of treatment and serum MMP-3 ... project and data analysis, and was primarily responsible for writing the manuscript KVA contributed to the design of the project, patient recruitment and management, and data collection ARS and AM...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... (1041, 1042) and their host TA strains (98, 100) 2-Nitrofluoranthene, 1-nitropyrene and 1,8-dinitropyrene are tested without S9mix, Glu-P-1 and Trp-P-2 are tested with S9mix using TA98 and YG1041 ... 2,4-dinitrotoluen and 4-nitroquinoline N -oxide (4-NQO) are tested without S9mix, 2-aminoanthracene and benzo[a]pyrene are tested with S9mix using TA100 and YG1042 nitropyrene and 1,8-dinitropyrene ... fumigants, heavy metals, inorganic chemicals and natural toxins, etc These results may suggest that the utility and limitations of both the Ames test and 8-OHGua assay in detecting chemicals likely to...
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Tài liệu WorldDSL™ G.S Double Pair/Line Power G.SHDSL for CO and Customer Premises docx
... resettable alarm log showing first and last occurrence date and time, and number of occurrences for each alarm type Alarm Relay: Form C relay contacts NO, NC and COM Loopbacks: local or remote ... E1: G.821 and G.826 Performance Measurements: ES, SES, UAS, BBES, ESR, SESR, BBER E1 Alarms: LOS, LFA, RAI, RAIS PM History: 96x15 minute increments, 7x24 hour increments for both HDSL and E1 performance ... PRBS generator and BER meter from the customer premise or CO ends Power Input Power: DC -36 to -72 VDC or AC 90 to 264VAC 47 to 63 Hz Line Power Cover full reach for G.shdsl standard span One...
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Tài liệu Run your car on water and gas double mileage! pdf
... anywhere around the housing as shown in Figure 14, and mark a spot ¼” up from the edge of the end cap. Remove the end cap. Apply primer, and then cement, to the mating surfaces of the doubler and housing and attach the doubler to the housing with its lower edge at the ... The Water‐hybrid system is proven and has been implemented in various forms and varieties by engineers, mechanics, and hobbyists around the world. It is the end result of many years of testing and experimentation with a multitude of hydrogen generating systems based on the ... requiring natural gas or propane as a source of heat; thereby saving valuable and diminishing natural resources. Space heaters and furnaces are excellent candidates. Stoves and other cooking units such as barbecue grills can also be fueled by the water‐hybrid system. Such ...
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