sscp analysis for the detection of mutations in tumor tissue samples

Techniques in Aquatic Toxicology Volume II - Section 4 ppt

... throughout the vessel, including the flow rate of the infused stress agent, the density and temperature of the mixtures, the number and species of test fish in the arena, and the shape and volume of the ... from the tank in which the fish was exposed to maintain the oxygen saturation level Sampling consisted of taking length and weight measurements, drawing blood for clinical pathology, and taking tissue ... significant progress in the ease, utility, and affordability of obtaining, interpreting, and applying behavioral endpoints in a variety of applications from water quality monitoring to use in toxicity...

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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc

Danh mục: Báo cáo khoa học

... SBEIIa-D(A201T) the amino acid change induced by the EMS treatment is located in the region of the N-terminal domain of the glycogen branching enzyme family, reported to be essential for the size of the ... caused the loss of two of the seven amino acids involved in the binding and catalytic activity in normal SBEIIa protein, respectively Arg465 and Asp467 Analysis of SBEIIa-transcripts in the knock ... product showed the inclusion of the intron V, whereas the smaller one was found to contain a deletion of the first seven nucleotides of exon VI The presence of the intron V in the longer transcript...

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Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc

Danh mục: Báo cáo khoa học

... lnRθ of several linked loci for the inference of selection While the single-locus lnRθ-test is largely independent of the demographical past, the additional power of linked loci is balanced by the ... Diversity) The projects were funded by the Academy of Finland, the Ministry of Agriculture and Forestry in Finland, the Nordic Gene Bank for Farm Animals (NGH), and the Nordic Council of Ministers ... http://www.gsejournal.org/content/42/1/32 Page of 14 Figure Detailed view of the extent and significance of LD in the cattle populations using the Haploview 4.0 program Numbers in the blocks indicate the percentage of the LD metric...

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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf

Danh mục: Báo cáo khoa học

... microscope Stretching of these metacercariae was done by hot formalin for two minutes Then, they were kept in Eppendorf tubes with neutral formalin (4%) Diagnosis of parasitic infections * Diagnosis ... saline pH 7.0) The internal organs were exposed after a vertical incision was made from the anal opening to the Before any dissection, the exterior of the fish was observed under the dissecting ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the...

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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields" pps

Danh mục: Báo cáo khoa học

... ta nim 03 rof detabucni erew setalp eht ,yltneuqesbuS llew hcae ot dedda saw noitulos etartsbus )]dica cinoflus enilozaihtzneb lyhte-3[id-oniza-2,2( STBA lµ 001 ,gnihsaw retfA h rof esadixorep ... syad evitucesnoc rof yad/gk/gm 02 ta stibbar ot ylralucsumartni deretsinimda saw nicymanaK nicymanak fo noitartsinimda ralucsumartni retfa amsalp tibbar ni nicymanak fo eliforp noitelpeD elbaT ... etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc raey-ruof a fo stluser :nicymanak...

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Báo cáo y học: "Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis" pps

Danh mục: Báo cáo khoa học

... from the baseline 80° of ≥ mm from the baseline, occurring two or more times, • a single radical deviation away from the baseline 80° episode of ≥ mm from the baseline A marginal tracing was defined ... and Development of 3DMP Research into the theoretical models underlying 3DMP began in 1976 in the People’s Republic of China in a project that investigated the effect of noise Int J Med Sci 2007, ... combination of these six transformations and the resulting 166 indexes does To reach the final diagnosis, the index patterns of the individual subject or patient are compared to the patterns stored in...

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Báo cáo y học: " Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis after coronary revascularization" doc

Danh mục: Báo cáo khoa học

... from the baseline 80° of ≥ mm from the baseline, occurring two or more times, • a single radical deviation away from the baseline 80° episode of ≥ mm from the baseline A marginal tracing was defined ... of ECG data contain idiopathic extrema that deviate from the baseline by ≥ mm, • in a 25-mm section of waveform in any 5.12-second segment of the ECG data, the waveform strays from the baseline ... histogram, all of which is used to Int J Med Sci 2008, generate indexes of abnormality The resulting patterns of the indexes are then compared for abnormality to the patterns in the reference...

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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx

Danh mục: Báo cáo khoa học

... test the null hypothesis of marginal symmetry, namely that the probability of an observation being classified into cell [i,j] in the contingency table is the same as the probability of being classified ... infected in the routine samples, there was a good correlation between routine samples and 5-sock -samples A lower number of Salmonella positive socks in the routine samples than in the 5-sock -samples ... more either by bacteriological analysis (nine flocks) or by positive serological reactions (2 flocks) before inclusion in the study Samples investigated: The samples investigated for all the 41...

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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)

Danh mục: Y học thưởng thức

... stenosis from the individual centers included in the meta -analysis The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile ... measured by the MCG device The resulting mathematically integrated patterns of the abnormal indexes are then compared for their degree of abnormality to the abnormal index patterns in the reference ... according to whether the patients had prior revascularization The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile...

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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens

Danh mục: Môi trường

... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... recommend the Ames test using these sensitive strains (especially YG1041 and/or YG1042) in combination with the standard tester strains (TA98 and TA100 series), in order to detect the mutagenicity of ... ogttST::Cmr) strain (deficient in O6-methylguanine DNA methyltransferase) sensitive to alkylating chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences...

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ag doped wo3-based powder sensor for the detection of no gas in air

Danh mục: Vật lý

... patterns (the lack of their long-range order and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar ... when the temperature of the Ag catalyst is increased to 250 8C, the sensitivity of the WO3 sensor kept at 150 8C 71 increased by three times Without heating the Ag catalyst or bypassing the gas ... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18]...

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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx

Danh mục: Điện - Điện tử

... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... number of random PBMC samples from macaques at the WaNPRC for the presence of an RV2 rhadinovirus We detected RV2 rhadinovirus DNA in of 30 macaques; of 20 M nemestrina, of M fascicularis and of...

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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot

Danh mục: Hóa học - Dầu khí

... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... number of random PBMC samples from macaques at the WaNPRC for the presence of an RV2 rhadinovirus We detected RV2 rhadinovirus DNA in of 30 macaques; of 20 M nemestrina, of M fascicularis and of...

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Báo cáo hóa học: " Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models" pptx

Danh mục: Hóa học - Dầu khí

... to the matrix X L (L being the iteration number), in the order of the highest to the lowest sensitivity To make the adjustment of the net influence of each of the remaining parameters on the ... availability of new information during the parameter estimation in UKF correlates to a general decrease in the uncertainty within the system [21], making the standard deviation a feasible choice for the ... used for the comparison Four values are plotted for each parameter in the bar chart of Figure The first bar represents the actual value of the parameter as determined in [12] The remaining bars...

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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf

Danh mục: Báo cáo khoa học

... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of ... 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in the intestinal tissues from naturally infected piglets Vet Pathol 2000,...

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Báo cáo khoa học: "Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine" pptx

Danh mục: Báo cáo khoa học

... saw noitcaer cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni ... 1.0/05DICT 002 gniniatnoc noisnepsus suriv 9981VK a fo emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ... sisongaid -ores eht ni esu laitnetop sti rof detagitsevni saw ASILE-I VEJ eht rof depoleved saw dohtem lacigolores tneinevnoc dna elpmis a ,nosaer siht roF selpmas fo rebmun detimil a htiw noitazidradnats...

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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx

Danh mục: Báo cáo khoa học

... sensitivity of the assay The results of our study indicated that the lateral flow biosensor assay was effective, even in the detection of 10 MAP organisms in the spiked fecal samples Apart from the spiked ... with μl of synthetic DNA with different concentrations ranging from 1-1,000 fmol μ/l The limit of detection was determined using the signal obtained for the negative control plus three times the ... fecal samples containing 10 organisms of MAP RFR: reflectometer reading, CFU: colony forming units A biosensor assay for the detection of Mycobacterium avium subsp paratuberculosis in fecal samples...

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Báo cáo y học: "Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis" pps

Danh mục: Báo cáo khoa học

... changes in secreted proteins, such as cytokines and chemokines Conclusion In conclusion, the findings of the present study show the reliability of ST analysis using a DIA system for the evaluation of ... levels regarding the detection of stained antigen, nuclear staining and background staining After the analysis, all observers independently calculated the mean change in the total number of positive ... out of the 10 patients in the prednisolone group responded versus none of the nine patients in the placebo group To determine whether the same observer identified the same differences in the...

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Báo cáo khoa học: "Study protocol of the German "Registry for the Detection of Late Sequelae after Radiotherapy in Childhood and " ppsx

Danh mục: Báo cáo khoa học

... centres of the GPOH study trials The documentation forms of these patients are asked for in the departments of radiotherapy if no documentation has been performed Parents' declarations of consent for ... have been integrated into most of the study protocols of the GPOH that are relevant in view of radiotherapy Therefore additional declarations of consent are not necessary in Germany The first ... study and are involved in continuing optimization NW is the study chairman of RiSK, developed the design of the registry study, is involved in continuing optimization and helped to draft the manuscript...

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Báo cáo sinh học: "Optimal design for the detection of a major gene segregation in crosses" potx

Danh mục: Báo cáo khoa học

... emphasized These curves are nearly linear for power under 70% and, in this linear part, the slope (ie the gain in power = = = per extra individual measured) increases with a The resulting increase in ... of dominance, before the experiment is designed, then maximum power will be attained by limiting the segregating subpopulation to the single backcross showing segregation However, the power of ... is the opposite of the homoskedastic case where the non- segregating populations were useful This result shows that the information in the non-segregating population derives from the level of the...

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