split horizon is disabled by default on frame relay physical interfaces
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
Ngày tải lên :
18/02/2014, 18:20
... kDa band was excised. The
sample was analysed using Q-TRAP MS by the Protein
Analysis Laboratory at the Cancer Research UK London
Research Institute (London, UK).
Immunoprecipitation
Cells were ... was clarified by centrifugation at
15 000 g for 30 min followed by centrifugation at 100 000 g
for 1 h. Saturated ammonium sulfate solution (200 mL)
was added (33% final concentration) and incubated ... phosphorylation of CDK4 on Y17 is
mediated by Src family kinases.
Results
Detection of CDK4 Y17 phosphorylation and
kinase activity
The N-termini of CDKs 1, 2, 4 and 6 are highly con-
served and there is...
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Moment and Shear, Generated by DL on the Composite Section
Ngày tải lên :
29/09/2013, 12:20
... Generated by DL on the Composite Section.
This generator is capable of handling from 1 to 10 spans, and is capable of returning values for continuous
sections. This is done by moment distribution. ... span point
column 1 = moment
column 2 = shear
Based on continuous section, constant inertia.
1 1.2 1.4 1.6 1.8
0
500
1000
mc
n8
1
n8
sp
+
disp
0 1 2
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
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24
25
26
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31
32
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35
36
37
38
39
1 ... of the design lane.
Combination 3: For both the negative moment between points of contraflexure under a uniform load on all spans, and
reaction at interior piers only.
90% of two design trucks...
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KRONE - What is Patch by Exception
Ngày tải lên :
26/10/2013, 19:15
... of tomorrow.
WHAT IS PATCH BY EXCEPTION?
Patch by Exception is a product feature of KRONE's HIGHBAND 8 and 10 modules that allows the normal cross
connect configuration to be wired using ... in configuration is
required the new connection can be simply made by plugging a HIGHBAND patch cord into the module to
instantly set up the new configuration by overriding the jumpered connection. ... progressive connectivity company is introducing
"Patch by Exception", the next generation in cable management systems.
Patch by Exception guarantees network managers a system that is not only...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
Ngày tải lên :
15/02/2014, 01:20
... corre-
sponds to the 40 kDa form, consists of the N-terminal
region (N) and one Kunitz domain (K1), whereas
HAI-1–NK1LK2, corresponding to the 58 kDa form,
consists of the N-terminal region (N), ... separated by SDS ⁄ PAGE under reducing conditions (B) or under nonreducing conditions (C), and analysed
by immunoblotting with the anti-c-Myc IgG. The protease domain of TMPRSS13 was quantified by scanning ... tissues
producing it. In response to tissue injury, such as
hepatic and renal injury, the inactive single-chain HGF
is converted to a two-chain form exclusively in
the injured tissue. This conversion...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... followed by Strep-tag II
was isolated by BamHI digestion and inserted into the
BamHI site of pmGFP–calpain 7.
To construct pCMV3xFLAG–IST1 and pGEX–IST1, an
IST1 cDNA fragment was amplified by PCR, ... intermolecular reac-
tion occur in the autolysis. Therefore, it is premature
to draw conclusions regarding the mechanism of the
enhancing effects of IST1 on mGFP–calpain 7–Strep
autolysis in vitro.
Both ... cultured
cells. On the other hand, overexpression of FLAG–
IST1
DMIM1,2
suppressed the autolysis. This observation
might be explained by regarding IST1 as a regulator of
the intracellular localization of...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
Ngày tải lên :
18/02/2014, 12:20
... all
incubation conditions. When rhTLK1 is diluted from
high storage concentrations to low assay concentra-
tions of 23 ngặmL
)1
, which is below the limit for
the ATP tetramerization effect, the ... respectively, depending on pre-assay incubation with ATP. Here,
the part of ATP that is necessary for tetramerization and how the reaction
velocity is influenced by the enzyme concentration are determined. ... scintillation
counting.
Analysis of kinetic data
Kinetic data were fitted by non-linear regression analysis to
the MichaelisMenten equation v ẳ V
max
ẵS=K
m
ỵẵSị
or the Hill equation v ẳ V
max
ẵS
n
=K
0:5
n
ỵẵS
n
ị...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
Ngày tải lên :
18/02/2014, 13:20
... light on the
function of this interaction in the in vivo situation.
By influencing the duration of the Ca
2+
signal of
ORs in the cilia of the sensory neurons, MUPP1 could
have a strong influence on ... interaction was
detected using MUPP1 antibodies.
Immunohistochemistry
Mice were raised and maintained according to governmen-
tal and institutional care instructions. Immunohistochemis-
try was ... proportion of transiently transfected cells
responding to odorant in ratiometric calcium imaging
experiments [24–26]. This alteration in the subcellular
distribution of MUPP1 upon co-expression with...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... formation by transferring electrons
from NADPH onto oxygen molecules [56]. A defici-
ency of one PHOX subunit in NADPH oxidase leads
to the inhibition of superoxide generation, and results
in chronic ... cytokine synthesis
and febrile response induced by SEC1 is dependent on
NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄
FLAP because these phenomena are attenuated by
apocynin and not by ETYA, MK ... localization of epitopes on
tryptic fragments. Infect Immun 45, 281–285.
5 Cremonesi P, Vimercati C, Pisoni G, Castiglioni B,
Luzzana M, Ruffo G & Moroni P (2006) Identification
of enterotoxin genes...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
Ngày tải lên :
19/02/2014, 06:20
... were isolated as a single peak fraction by
a 0.1–1.0 m NaCl linear gradient in R buffer. Histone H1,
which exists in the DNA affinity fraction, was separated as
a major component stepwise elution ... histone H1 [14],
topoisomerase II [15], lamin B1 [16] and SAF-A
(hnRNP U) [17].
It is known that the aggregation of DNA is com-
patible with the functions of proteins under in vitro
conditions. ... and one of the
candidates may be linker histone H1 of an aggregative
protein. IR provokes ATM-dependent dephosphoryla-
tion of histone H1 [42]. A recent study demonstrates
that whereas histone...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
Ngày tải lên :
20/02/2014, 02:21
... mm
Tris ⁄ HCl (pH 7.5). After incubation for 1 h at 4 C,
20 lgặmL
)1
of heparin was added and the incubation was
continued for 5 min. The complex formation was analysed
by electrophoresis on nondenaturing ... protein containing a fused N-terminal histidine tag
was purified by binding to and elution from Ni-NTA-affinity
matrix [43].
Nuclear extract preparation and fractionation
of the B. mori transcription ... mutual contamination. In
every fractionation the quantities of fractions had to
be optimized because use of larger amounts of any
individual fraction tended to result in inhibition of
transcription....
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... in
transcriptional control and their impact on cellular function.
Oncogene 19, 2468–2473.
45. Tone, M., Powell, M.J., Tone, Y., Thompson, S.A. & Waldmann,
H. (2000) IL-10 gene expression is controlled ... prothrombinase. This combination of consti-
tutive expression and induction may permit a rapid response
to inflammation. However, the mechanisms regulating fgl2
expression, either constitutive or ... demonstrated that
Fig. 7. EMSA analysis of the function of Ets/Stat3 cis elements. (A) Cross competition of consensus Ets/PEA3, Ets/PU1, and Stat3 cis elements
with corresponding transcription...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
Ngày tải lên :
21/02/2014, 01:21
... for a region of this construct
containing galA and some of the flanking regions,
resulting in the genomic sequence of A. niger galA.The
presence of one putative intron was confirmed by RT-
PCR ... start codon. In addition, one putative PACC
site ()549) [31] and two CCAAT sites ()280, )300) were
detected.
Expression analysis of
galA
The expression pattern of galA on a selection of mono-
saccharides, ... library of A. niger using
this fragment as a probe resulted in the isolation of two
galA-containing phage k clones. From one of these clones
a4.5-kbEcoRI fragment was cloned into pBluescript
SK
+
,...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... metal.
In all structures solved for the mononuclear SODs, the
metal ion is held in place by an absolutely conserved
quartet of residues comprising three histidines and one
aspartic acid which act as ligands ... no
other SODs with this mutation exist naturally. The nature
of this phenomenon still remains elusive.
Metal specificity may be considered as consisting of two,
presumably distinct, stages. First, ... peroxide
on the iron-containing superoxide dismutase of Escherichia coli.
Biochemistry 26, 1251–1257.
47. Misra, H.P. & Fridovich, I. (1978) Inhibition of superoxide dis-
mutase by azide....
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... suggested that
LBR binds to a DNA region on chromatin in the RS region.
This idea was consistent with a report by Ye & Worman
[15], i.e. that a region corresponding to RS binds free DNA.
Duband-Goulet ... chromatin is
regulated by phosphorylation in the arginine-serine repeat-
containing region (RS-region) by multiple kinases. The
potential function of LBR and LAP2 in vesicle targeting to
chromatin is ... Expression of fusion proteins was induced by
the addition of 0.1 m
M
isopropyl thio-b-
D
-galactoside,
followed by incubation for 6 h at 30 °C. The bacterial
cells were collected by centrifugation...
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