sperm dna damage and pregnancy loss after ivf icsi
DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends, Cell 134(6):981-994 2 Synergy Between DNA Replication and Repair Mechanisms Maria Zannis-Hadjopoulos and Emmanouil ... locally unwound DNA (Bell and Dutta, 2002; Dutta and Bell, 1997) and promoting the assembly of the multienzyme complexes required 26 DNA Repair − On the Pathways to Fixing DNA Damage and Errors for...
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Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
... 7.14-9.29 0.943 Abbreviations: DSB/Gy /DNA unit = double-strand breaks induced per Gy and per 200 Mbp; RIA = radiation-induced apoptosis at 1, and Gy after 24 hours a and b are the constants that define ... experiments and data acquisition MIN has been involved in conception and design of the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of ... predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new data which may help to understand and define the complex mechanisms behind the...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
... causes DNA damage in hepatocytes Transplant Proc 26, 907–909 Moller P, Loft S, Lundby C & Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage ... As both hypoxia and reoxygenation have been reported to induce DNA damage [30–32], we examined the effects of pyruvate addition during hypoxia and after reoxygenation on DNA damage HepG2 cells ... single-strand and double-strand breaks Pyruvate reduces DNA damage during ⁄ after hypoxia Cells cultivated in Petri dishes (25 mm in diameter) were suspended in 0.5 mL of NaCl ⁄ Pi, and cell density...
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báo cáo hóa học:" Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)" pptx
... Following DNA damage recognition, downstream DNA repair proteins (XPA, RPA, TFIIH complex, ERCC1/XPF and XPG) are recruited to the DNA damage recognition complexes in both TCR and GGR to remove the damage ... mouse/cage) and monitored for recovery and discomfort Immediately (0 h) and 24 h after perfusion, mice were euthanized and tumors, kidneys, liver, spleen, heart and brain were dissected and snap ... and CSB along with RNA pol II recognize damage, whereas in GGR, XPC and DDB2 are important for lesion recognition XPC is actively involved in the recognition and initiation of cisplatin -DNA damage...
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Checkpoint and Coordinated Cellular Responses to DNA Damage
... interaction between ATR-ATRIP and RPA-ssDNA, and might contribute to the activation of ATR Transduction of DNA Damage Signals After ATM and ATR are activated by DNA damage, they phosphorylate numerous ... types of DNA damage and defective in certain checkpoint responses Although the methylations of H3 and H4 are not induced by DNA damage, the changes in chromatin structure after DNA damage might ... ATR-ATRIP, Rad17, and 9-1-1 complexes can independently recognize damage- induced DNA structures such as ssDNA and junctions of dsDNA/ssDNA Moreover, RPA appears to play important roles in the damage recognition...
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Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt
... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated ... cellular context and the extent of DNA damage – which determines whether or not damage is reparable – the activated DDR can trigger different cellular responses Mild DNA damage is usually handled through ... turn determines whether DNA lesions can, or cannot, be repaired As unrepaired DNA damage is characterized by constant activity of the DNA damage checkpoint kinase ATM and ⁄ or ATR, continuous...
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in situ detection of dna damage, methods and protocols
... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage with ... single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA This is particularly useful since many single-strand breaks are also introduced into DNA ... study of DNA damage holds a wide interest within both basic and applied fields of research Elucidating the mechanisms involved in the generation of DNA damage, and the consequences of this damage, ...
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Báo cáo khoa học: "Pregnancy loss in dairy cows: the contributing factors, the effects on reproductive performance and the economic impact" doc
... detroper )%6.01 dna 2.01( setar eht naht rewol si ti dna )]82,31[ ;%6.3 dna 4.0( setar detroper ylsuoiverp emos naht rehgih si %9.6 dna ,seiduts neewteb ylediw yrav seod LP fo ecnedicni dna etar detroper ... nosboD dna yrrebsroB dna ,]81[ gnaK dna miK yb detacidni sa ,sitirtemodne fo ecnedicni desaercni eht ro /dna noitulovni eniretu deyaled htiw detaicossa eb thgim siht ,niagA sretsemirt dnoces dna ... ni si siht dna ,shtnom remmus eht gnirud naht retniw ylrae dna nmutua gnirud retaerg saw LP fo ecnedicni eht taht detartsnomed ]42[ riN-dlefsukraM ,dnah rehto eht nO ]3[ rebmetpeS dna yluJ ni...
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Báo cáo khoa học: "Radiation induced apoptosis and initial DNA damage are inversely related in locally advanced breast cancer patients" ppt
... between initial DNA damage, measured as DSB, and RIA Ionizing radiation (IR) kills cells by damaging the structure and function of genomic DNA The response of cells to this damage and their ability ... initial DNA damage Mean ± standard deviation of DSB/Gy /DNA unit, obtained from our files [10] was 1.70 ± 0.83 (range 0.634.08; median, 1.46) No relation was found between the number of DSB and the ... were held hour at 4°C and incubated at 37°C for 24 hours The study of initial DNA damage was completed in the University of Granada (Spain) Initial radiation-induced DNA damage in PBLs was measured...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
... double-strand DNA damage and complex with other important repair molecules g-H2AX is required for foci formation for numerous factors including p53, MRN complex (MRE-11, RAD50, and NBS1), and BRCA1 ... phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines Cancer Res 2004, 64(19):7144-7149 47 Bilsland E, Downs JA: Tails of histones in DNA double-strand break ... prior to irradiation and were stained at 24 and 48 h postirradiation (2 Gy) Both adherent and detached cells were collected, centrifuged, and double stained with Annexin V-FITC and propidium iodide...
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Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... comparison between Br and Bo, Bo and Bna, and Br and Bna showed very similar Ks distribution with the two peaks for 4R and 2R at similar Ks modes as those in Br-Br paralog analyses, but loss of a peak ... data, and wrote the manuscript SJK and TJY designed research and contributed analytic tools SJK, MJ, JAK, MHL, JSK, KBL, and SIL contributed to data acquisition YJS and SB developed the database and ... triplicate regions (A2, A3, and A10) However, in At5, blocks R and W were separated on the short arm and long arm, respectively [38,39] Similarly, blocks E and N were adjacent and triplicated in Br...
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Báo cáo y học: " Physical and in silico approaches identify DNA-PK in a Tax DNA-damage response interactome" pot
... which DNA- PKcs is a member (DNA- PKcs, PCNA, PARP, Ku70, Ku86, and TRBP) Thus, DNA- PKcs demonstrates an increased rank when consideration is refocused toward protein interactions involved in DNA damage ... peptide and the complexes separated on SDS-PAGE and subjected to immunoblotting with antiDNA-PKcs Endogenous DNA- PKcs specifically associates with the Tax containing protein complex and is detected ... Figure Tax binds to DNA- PKcs HTLV-15 HTLV-1 Tax binds to DNA- PKcs The fusion proteins STax and S-GFP were isolated from 293T cells as described and analyzed for co-precipitation with DNA- PKcs Shown...
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Báo cáo y học: "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export" pps
... (upper Tax band) to total Tax (upper and lower Tax bands) for both unirradiated and UV irradiated cells These results demonstrate that mono-ubiquitination of Tax occurs following DNA damage and that ... to DNA damage may be a consequence of their inability to be ubiquitinated Lysine residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in response to DNA damage ... into 293 cells and then mock or UV irradiated 48 hours after transfection Lysates were prepared 30 minutes after irradiation, Tax was immunoprecipitated, and levels of Tax and UB-Tax and were examined...
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Báo cáo y học: "Dissection of a DNA-damage-induced transcriptional network using a combination of microarrays, RNA interference and computational promoter analysis" pot
... (uninfected and the LacZ control cells and cells knocked-down for Rel-A, p53 and ATM), each probed at two time points: without treatment and h after exposure to NCS Definition of the damage- responding ... uninfected cells and cells expressing siRNA against the bacterial enzyme LacZ and cells knocked-down for Rel-A, p53 and ATM), each probed at two time points: without treatment and hours after exposure ... clustering after normalizing the rows to have mean = and SD = The heat map visually represents the normalized matrix after being clustered Red, green and black entries represent above-, below- and near-average...
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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE
... of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage increases following ... cytotoxicity and mutagenesis, respectively [21, 22] Here, we hypothesize that influenza infection induces DNA damage, and that DNA damage responses modulate cytotoxicity and tissue damage in infected ... an induction of DNA damage both during and after the phase of active influenza infection in lung cells To understand the spatiotemporal relationships among DNA damage, infection and inflammation,...
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Regulation of spindle behaviour by DNA replication and damage checkpoints in budding yeast
... checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage checkpoints In S.cerevisiae, the G1 DNA ... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage ... consequence of loss of this regulation Given the substantial overlap between the effectors involved in replication checkpoint and DNA damage checkpoint , we enquired whether DNA damage checkpoint...
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Oxidative and nitrosative DNA damage occurrence, measurement and mechanism
... Oxidative and Nitrosative Damage in DNA 1.2.1 DNA damage Induced by Reactive Oxygen Species 1.2.2 DNA Damage Induced by Reactive Nitrogen Species 1.3 Mutagenicity of Oxidative and Nitrosative DNA Damage ... obtained mtDNA of desired purity in order to make a valid comparison of oxidative damage between mtDNA and nDNA Our data show that a possibility exists that mtDNA damage is not higher than nDNA damage, ... quantities of DNA, cross contamination of nDNA and mtDNA, and artefactual oxidation of DNA during isolation of mitochondria Mitochondrial DNA constitutes only 1% of the total cellular DNA If mtDNA is...
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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE
... sense for DNA damages, activate cell signaling and repair DNA lesions DNA damage are surveyed via DNA damage sensors, Mre11-Rad50-Nbs1 (MRN) complex, and replication protein A (RPA) and Rad9-Rad1-Hus1 ... bases, deaminated bases, and DNA- DNA and DNA- protein cross-links (Lonkar and Dedon 2011) In addition, attack of ROS / RNS on DNA backbone can also lead to DNA single strand breaks (SSBs), which ... ONOO- and OH• will overwhelm antioxidant systems and exacerbate DNA damage OH• radical is considered one of the most potent ROS that leads to DNA damage by oxidizing bases and attacking DNA backbones...
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NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity
... 1.1 NBS and NBS1 protein 1.2 MRN complex 1.3 ATM and ATR kinases 1.4 DNA damage response 10 1.5 1.4.1 DNA damage sensing 10 1.4.2 DNA damage ... 2003) ATM and ATR are at the central of DNA damage signaling pathways About 25 substrates of ATM and ATR have been identified, and many of them have been revealed as candidates in DNA damage signaling ... DNA damage sensing DNA damage response begins with “sensor” proteins that sense DNA lesions/chromatin alterations after DNA damage induction This process is characterized by rapid formation of DNA...
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NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity
... 1.1 NBS and NBS1 protein 1.2 MRN complex 1.3 ATM and ATR kinases 1.4 DNA damage response 10 1.5 1.4.1 DNA damage sensing 10 1.4.2 DNA damage ... 2003) ATM and ATR are at the central of DNA damage signaling pathways About 25 substrates of ATM and ATR have been identified, and many of them have been revealed as candidates in DNA damage signaling ... DNA damage sensing DNA damage response begins with “sensor” proteins that sense DNA lesions/chromatin alterations after DNA damage induction This process is characterized by rapid formation of DNA...
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