... azoospermia rather than testicular dysfunction, it is not uncommon for relatively large Sperm Preparation for IVFandICSI 149 quantities of sperm to be obtained and subsequently used for IVF, ... motile and functionally competent spermatozoa while eliminating dead spermatozoa and other cells, including bacteria and leukocytes The technique should also minimize damage to the spermatozoa and ... fertilizing capacity of human spermatozoa Fertil Steril 1979; 31:321 Sperm Preparation for IVFandICSI 159 WHO Laboratory Manual for the Examination of Human Semen andSperm Cervical Mucus Interaction...
... efficacy of sperm separation methods (Sephadex, glass wool and Percoll) on sperm quality, such as motility, morphology and plasmamembrane integrity, and evaluating the effect of these methods on IVF ... SE (n = 28) Sperm separation techniques on IVF in cryopreserved bovine semen 253 Table Effect of glass wool filtration and Percoll separation of spermatozoa on IVF results Cleavage Spermatozoa ... effect and action of dead sperm on diluted bovine semen J Dairy Sci 1972, 55, 614-620 Shen H, Ong C Detection of oxidative DNA damage in human spermand its association with sperm function and male...
... inactive chromatin For example, minichromosomes assembled in Xenopus oocytes partition into inactive soluble chromatinand insoluble active chromatin [111] Early attempts to fractionate native chromatin ... 30-nm chromatin fibres versus open 10-nm chromatin fibres We also need to be able to define the specific mechanisms that create a more dynamic chromatin structure in which nucleosomes andchromatin ... create islands of active chromatin which counteract the repressive complexes that mediate heterochromatin spreading [71,72] Many BEs are known to have promoter activity and to recruit chromatin...
... into mitosis andchromatin was condensed, most of the MLL1 protein was dissociated from the chromatinand spread into the cytoplasm, generating a distinct footstep (gap) for condensed chromatin ... HoxA5, HoxA7 and HoxA10 genes Notably, HoxA5 expression was almost completely abrogated, whereas HoxA7 and HoxA10 were only partially down-regulated The partial down-regulations of HoxA7 and HoxA10 ... thank Saoni Mandal and Mandal laboratory members for critical discussions This work was supported by grants from the Texas Advanced Research Program (00365-0009-2006) and the American Heart Association...
... the local chromatin infrastructure through recruitment of HAT- and possibly HDAC-enzymes in the course of myogenesis CHROMATIN REMODELING IN MUSCLE DIFFERENTIATION Chromatin structure and nucleosomal ... for HMG1 and linker histones B4 and H1 in organizing chromatin EMBO J 15, 548–561 21 Ura, K., Nightingale, K & Wolffe, A.P (1996) Differential association of HMG1 and linker histones B4 and H1 with ... determination and differentiation: story of a core regulatory network and its context Curr Opin Cell Biol 8, 877–889 Kitzmann, M & Fernandez, A (2001) Crosstalk between cell cycle regulators and the...
... reconstitution and analysis, and cytological methods for imaging chromatin functions in vivo Volume 376 includes electron microscopy and biophysical protocols for visualizing chromatinand detecting chromatin ... regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin templates We are exceedingly ... is calculated from the and 00 and known Re (30.1 nm) of the T3 internal standard For the unknown band(s) of interest, the and 00 are determined experimentally and the Re in each running...
... histones andchromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... Health and Human Development, Unit on Chromatinand Transcription, Bethesda, Maryland 20892 Sonja Ghidelli (14), National Institute of Child Health and Human Development, Unit on Chromatinand Transcription, ... HHT1 and HHT2 (for histone H three), HHF1 and HHF2 (for histone H four), HTA1 and HTA2 (for histone H two A) and HTB1 and HTB2 (for histone H two B) HHT1 and HHT2 encode identical H3 proteins, and...
... histones andchromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... visualizing chromatinand detecting chromatin interactions, enzymological assays for histone modifying enzymes, and immunochemical protocols for the in situ detection of histone modifications andchromatin ... and defined chromatin segments, protocols for nucleosome reconstitution and analysis, and cytological methods for imaging chromatin functions in vivo Volume 376 includes electron microscopy and...
... these two proteins interact and work on both the viral DNA and host chromatin in the process of integration remain elusive Further mutagenic analysis for IN /chromatin and IN/LEDGF interactions ... bind chromatin in a LEDGF/p75-independent manner and is sufficient to sustain a low level of HIV-1 infection Materials and methods Cell lines and transfection Human embryonic kidney 293T and the ... only S1 (non -chromatin- bound) and S2 (chromatin- bound) fractions were analyzed by immunoprecipitation using an anti-GFP antibody and detected by WB with the same antibody Protein bands in each...
... shown for a region of chromosome 7q Grey and black regions on the chromosome indicate cytogenetic bands (G-band, black; R-band, grey) The blue and black bands under the chromosome indicate the ... by which TSA alters the association between chromatinand the nuclear periphery? Chromatin decondensation, and hence enhanced chromatin flexibility and mobility, as a consequence of the hyperacetylation ... associated regions shifted away from G-bands and toward R-bands (Figure 1b) This scale of genome reorganization should be detectable at the cytological level, and indeed, relocalization of loci either...
... the gene-oligo array and one (hypomethylated) target in the random-PCR array (Figures 1d and 2) In addition, we detected two targets of DRM1/2 in the randomPCR array (Table 1) and ten DRM1/2 targets ... cmt3 drm1/2 and observed extensive overlap for both the random-PCR array (Table 1) and the gene-oligo array (313 hypomethylated and 33 hypermethylated in cmt3 drm1/2; Figures 1e and 2) reviews ... drm1/2 and ago4 were in a Ler (clk-st) and crm3 drm1/2 was in a Ws wild-type background Gene-oligo and random-PCR datasets of targets are available with a graphical interface for browsing and for...
... of spermand its bearing on arthropod phylogeny In: Arthropod Phylogeny (Gupta A.P., ed.), Van Nostrand Reinhold, New York, 609642 Bauer H (1940) Uber die Chromosomen der bisexuallen und der parthenogenetischen ... of sperm types, with special reference to annelids and molluscs, and an example of spermiocladistics In: New Horizons in Sperm Cell Research (Mohri H ed.) Japan Science Society Press, Gordon and ... Normal spermatogenesis in Drosophila In: Biology of Drosophila (Demerec M ed.) 1-61, New York, Wiley Dybas L.K & Dybas H.S (1981) Coadaptation and taxonomic differentiation of spermand spermathecae...
... (develomental countries), ETS and maternal smoking during pregnancy www.milieu-en-gezondheid.be Methods (1) • SPE data on 345,081 singleton newborns • Interpolation method and population weighted on ... inhalation rate of 18 m³/day and a dose of 12 mg per cigarette www.milieu-en-gezondheid.be Conclusions • We noted associatons between fetal growth restrictions and particulate air pollution • Especially ... meta-analytic data comparison estimates for biomass, ETS exposure and maternal smoking were plotted against estimated average daily dose of fine particulate matter from these exposures www.milieu-en-gezondheid.be...
... Young and Sweeney using SDS ⁄ dithiothreitol-decondensed spermchromatin from rabbits and fowl [37] They are also very similar to the DNase I digestion patterns obtained with human spermchromatin ... Protamine-like proteins andchromatin A B C Fig The spermchromatin of M surmuletus consists of bundles of parallel DNA molecules (A) Electron microscopy of spread spermchromatin rotary shadowed ... as those from EM and X-ray diffraction of spermchromatin fibers (Fig 5), provide biochemical and biophysical evidence for the lack of a nucleosomally organized chromatin in the sperm of M surmuletus...
... context and links to patriarchal control, the different understandings across race, class (and, presumably, religion and other social markers) and its fluidity in time and space Young men and fatherhood ... Clowes, Toni D’Amant and Vuyo Nkani Teacher responses to pregnancyand young parents in schools 49 Deevia Bhana and Sisa Ngabaza SECTION B Learner attitudes to pregnancy, parents and gender equality: ... condom use by women and men and among those from the richest and poorest households’.1 As the MDGs indicate, teenage pregnancyand its corollary, young parenthood, are a particular problem when...
... Standards and Protocol Therefore, there is the need to enhance their capacities to teach the present pre-service students the necessary skills to offer CAC and understand the relevant laws and ... Policy to include PAC and the provision of CAC as permitted by law [11] The 2006 GHS Reproductive Health Standards and Protocol provide guidelines for interpreting the law and these are consistent ... post abortion care and legal pregnancy termination Specifically, this study focuses on the capacity and willingness of midwifery tutors to teach contraception, post abortion care and legal termination...
... Emmanuelle Mathieu d’Argent, Karine Morcel, Joelle Belaisch-Allart, Michelle Nisolle, and Emile Daraý (2012) Cumulative pregnancy rate after ICSI -IVF in patients with colorectal endometriosis: results ... Pelinck, N.E.A Vogel, E.G.J.M Arts, A.H.M Simons, M.J Heineman and A Hoek (2007) Cumulative pregnancy rates after a maximum of nine cycles of modified natural cycle IVFand analysis of patient dropout: ... undergoing ICSI: a multicenter study Middle East Fertility Society Journal Vol 13, No pp.63-66 M.J.Pelinck, N.E.A.Vogel, A.Hoek, A.H.M.Simons, E.G.J.M.Arts, M.H.Mochtar, S.Beemsterboer, M.N.Hondelink and...