specificity membrane binding and bilayer insertion
Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
... membrane, accepts a lipoprotein from LolA and somehow incorporates it into the outer membrane The overall structures of LolA and LolB are very similar [4] They comprise 11 antiparallel b-strands, ... b-barrel, and two loops covering the barrel The barrel and the loops containing three a-helices form a hydrophobic cavity, which has recently been found to undergo opening and closing on binding and ... centrifugation, and analysed by SDS–PAGE, followed by immunoblotting (Fig 4A) OmpA and SecG were examined as markers of the outer and inner membranes, respectively Four outer membrane- specific...
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx
... FEBS 2003 16S rRNA- and membrane- binding domains of Era (Eur J Biochem 270) 4165 membrane- binding activities The results of this study indicate that the RNA- and membrane- binding domain is a ... function and whether, biochemically, the RNAand membrane- binding domains overlap To investigate whether the RNA- and membrane- binding activities require the GTPase activity of Era, and whether ... responsible for RNA binding is also required for membrane binding [20] However, it was not clear whether the membrane- binding domain of Era also required the GTP -binding and GTPase activities...
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Data Binding and Silverlight List Controls
... ObservableCollection GetHands() { 126 CHAPTER ■ DATA BINDING AND SILVERLIGHT LIST CONTROLS ObservableCollection hands = new ObservableCollection(); hands.Add( new StartingHands() ... Simple data binding example 106 CHAPTER ■ DATA BINDING AND SILVERLIGHT LIST CONTROLS Create a new Silverlight application in Visual Studio 2008 Name the project BasicDataBinding, and allow Visual ... two-way data binding We will now move on to look at data binding lists of data to the two list controls provided in Silverlight: DataGrid and ListBox 113 CHAPTER ■ DATA BINDING AND SILVERLIGHT...
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Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf
... Yersinia Membrane targeting and pore formation by the T3SS (YopB) and pathogenic Escherichia spp (EspD) all carry two predicted TM regions, and are predicted to have a N-terminal coiled-coil region and, ... Host membrane characteristics and response to pore formation The composition of the host cell membrane appears to be a critical point for the invasion of bacteria, insertion of translocators and ... eukaryotic membrane, toxins produced in the bacterial cytoplasm release their cognate chaperones and are injected through the translocon pore and into the target cytoplasm IM, inner membrane; ...
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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... affect TRBP and Dicer binding strand selection and loading into the RISC This is beneficial both in achieving strong silencing and also minimizing off-target silencing by the passenger strand [45] ... Effect of guide strand 5¢ end mismatch on TRBP and Dicer binding Having observed variability in the impact of silencing TRBP and Dicer on the function of the fully paired and mismatched sequences, ... affect TRBP and Dicer binding A B H K Kini and S P Walton C of TRBP in this complex Binding reactions performed in extracts after TRBP silencing showed a concomitant reduction in binding at the...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... vacuolar membranes Results Membrane- binding properties of IC In an attempt to detect and characterize the membrane binding of IC, a member of the PEBP family, we first performed a liposome -binding ... for anionic membranes containing PG [16], and little information is available regarding the phospholipid and membrane binding of the other members of the PEBP family Consequently, the binding characteristics ... of the binding of IC to phospholipid membranes, are currently unclear and remain to be clarified The present in vitro membrane- binding analyses of IC permitted the phospholipid specificity and phospholipid...
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Tài liệu Báo cáo khoa học: Tet repressor mutants with altered effector binding and allostery docx
... The DNA fragments containing the randomized codons 14–25 (C-terminal part of helix a1 and the following loop) and 93–102 (helix a6 and the b-turns N-terminal and C-terminal of a6) as described ... wild-type TetR, but leads to loss of DNA binding and 4-ddmaatc dependent corepression in TetRi2 V99T, on the other hand, shows partial DNA binding, corepression by atc and induction by 4-ddma-atc when ... in helices a1, a4, and a6 [8], we decided to combine randomizations in these helices with TetRi2 (containing the mutations H64K, S135L and S138I) We screened the resulting candidates for TetR...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... Primers and 3, primers and 3, and primers and were used to amplify the genes encoding Tma-RNase HI, Tma-CD and Tma-ND, respectively The resultant DNA fragments were digested with NdeI and HindIII ... binding affinities and enzymatic activities of Hsa-RNase H1 [22,26] and mouse RNase H1 [24] To examine whether the corresponding tryptophan residue (Trp22) is important for substrate binding and ... three-stranded anti-parallel b-sheet (b1–b3) and two helices (aA and aB) [22] It binds to the minor groove of the substrate, such that a loop between aA and b3 interacts with the RNA backbone and...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... aliquots, and washed twice with 200 lL of cold binding buffer Nitrocellulose membranes were exposed to film, and each spot was cut out and counted by liquid scintillation ATP hydrolysis and TLC ... of binding buffer, and added to biotinylated IRE (10 lg in 150 lL of binding buffer) Biotinylated IRE was bound to streptavidin beads for 10 at °C, and unbound IRE was washed with 100 lL of binding ... each sample and were compared to a standard curve of ATP solutions ranging from 10 to 750 nm Statistical methods Filter -binding assay ATP -binding activity was determined by a filter -binding assay...
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Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot
... Summary of the GTPcS and GDP dissociation constants and stoichiometrical binding site (n) for wild-type SEPT2 and S218A mutant The data for SEPT2 were best fit with a single binding model while ... the Kd, koff and kcat of septins and members of the Rho family could be taken to imply that septins require GTP exchange and GTPase activating proteins to complete the GTP binding and hydrolysis ... pH 7.5 and 40 mm MgCl2) and filtered through a 25-mm pre-wetted 0.2 lm nitrocellulose filter membrane (Millipore, Cambridge, ON, Canada) The membranes were washed with mL of stop buffer and the...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
... modifications [23] To assess hLf binding to surface and/ or cytosolic nucleolin, we isolated the protein from the extranuclear pool and investigated the binding parameters and kinetics in a surface plasmon ... (Fig 7A) and HB-19-fluo (Fig 7B) A marked binding competition occurred between either HB-19-fluo and 8-molar excesses of hLf (84% inhibition of HB-19-fluo binding) (Fig 7B) or hLf-biotin and 50-molar ... that constitutes the binding site for ligands of nucleolin, such as HB-19 [26], midkine [19] and Lf (the results herein) In view of their efficient binding to surface nucleolin and to their weak...
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Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx
... loop between membrane- spanning segments V and VI and the region, termed Xa, comprising the cytoplasmic loop between TMS X and XI and part of TMS XI Studies of the citrate ⁄ lactate and malate ⁄ ... (red) and the positions of the membrane spanning segments were indicated below the profile (I-XI) The N- and C-terminal halves contain six and five TMSs, respectively Unfortunately, the N- and C-terminal ... I-XI and loops Vb and Xa were indicated at the bottom Bottom: local alignment between the N- and C-terminal of BH0400bhal In between, identical (*) and similar () residues were indicated Above and...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... activities (X) and Ca2þ uptake (B) in a variety of microsomes are presented: (A) and (B), skeletal muscle SR; (C) and (D), cardiac SR; (E) and (F), platelet microsomes; and (G) and (H), cerebellar ... following equation: ½E þ ½L $ ½EL Where [E] and [L] are the concentrations of free sites and ligands, respectively, and [EL] is the concentration of bound ligand The total concentration of sites can ... 0.45-mm Millipore HA filter, and placed in scintillant for counting This was carried out in the absence and presence of curcumin, and controls to estimate nonspecific binding of ATP to the filter...
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Water-Quality Data from Semipermeable-Membrane Devices and Polar Organic Chemical Integrative Samplers Deployed in the McKenzie River Basin, Oregon pptx
... control Relative standard deviation Size exclusion chromatography Semipermeable membrane device U.S Geological Survey Water-Quality Data from Semipermeable -Membrane Devices and Polar Organic Chemical ... semipermeable membrane device (SPMD) and the polar organic chemical integrative sampler (POCIS)—are being used to address these particular challenges Both the SPMD (Huckins and others, 2006) and POCIS ... Setting and Data‑Collection Sites The environmental setting of the McKenzie River basin and a discussion of sampling sites in the basin have been previously reported in McCarthy and others (2009) and...
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Báo cáo khoa học: Intrinsic local disorder and a network of charge–charge interactions are key to actinoporin membrane disruption and cytotoxicity ppt
... N-terminus and the other binding regions of StnII Also, Y111 is crucial for membrane binding as it is located at the POC binding site It was shown previously [16] that the two mutations R29Q and Y111N ... changes compromise regions that are important for membrane interaction (loop 105–113 and helix-a2 and its surroundings) and insertion (N-terminus end and loop 25–29), as described above Interestingly, ... composed by b-strands 145–150 and 156–161 and they are rich in hydrophobic and exposed residues In particular, the aromatic rings of Y140 and W146 have high accessible surface area (30% and 35%, respectively)...
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Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx
... characteristic pH-dependent binding to both ligands In addition, no binding to murine IgG1, and only weak binding to murine IgG2b at pH 6.0, was observed, and this stringency binding is characteristic ... two parallel and independent interactions (KD1 and KD2) take place between the ligand and the receptor, and the derived affinities were determined to be 6.7 ± 0.1 · 10)9 m (KD1) and 219.0 ± 37.7 ... kinetics, and thus two relevant models were explored – the heterogeneous ligand -binding model and the bivalent analyte model – both supplemented with the BIAevalution Wizard The heterogeneous ligand-binding...
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Báo cáo khoa học: Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies pot
... Y61 and increases the distance between the hydroxyl group of Y61 and Ca of the ligand In Gly, l-Ser and l-alloThr complexes with Y51F bsSHMT, the hydroxyl group of Y61 and Ca of the bound ligand ... et al Role of Y51 and Y61 in bsSHMT catalysis PLP content and activity measurements of Y51F, Y61A and Y61F bsSHMTs The purified Y51F and Y61F bsSHMTs were nearly colourless and pale yellow, respectively, ... structural and functional studies on Y51F, Y61F and Y61A bsSHMTs These 4608 studies suggest that Y61 is a possible candidate for proton abstraction from Ca of Gly and 3-hydroxy amino acids, and that...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... functional and structural characterization of ETB and similar GPCRs Further analysis of the identified ETB131 and ETB93 fragments will help to identify residues involved in ligand binding and dimerization, ... of chimeric ETB derivatives and binding of antagonists, Wada et al proposed a 60 amino acid area spanning I138-I197, and thus covering TMS2 and TMS3, as the ET-1 -binding site [44] In addition, ... regions of ETB still might contribute to the ligand binding Evidence for several and partially overlapping binding sites of ETB for different ligands has been documented [46] FEBS Journal 274...
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Báo cáo khoa học: Identification and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx
... endogenous ligand which binds to site II, and of cefazolin (site I-ligand) and verapamil (site I and II-ligand) as exogenous ligands All these ligands are known for their high binding efficiencies ... reduced and oxidized HSA, we examined two functional properties, ligand -binding properties and antioxidant activities, using both purified nonoxidized and oxidized HSA samples Ligand -binding properties ... ligand While l-Trp and cefazolin showed decreased affinity to mildly oxidized HSA, verapamil binding to mildly oxidized HSA was Table Binding of L-Trp, cefazolin and verapamil to purified HSA and...
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Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx
... isoforms in distinct intracellular membranes, targeting DMPK isoforms A and B to the endoplasmic reticulum (ER) and DMPK C and D to the mitochondrial outer membrane (MOM) [5,24] Specific elements ... polymerase and specific primers EcoRI and XhoI sites were incorporated in the forward and reverse primers, respectively (underlined, see below) DNA fragments were cut with EcoRI and XhoI, gel purified and ... coiled-coil domain is crucial for homo- and heteromultimerization of DMPK isoforms and that multimerization has a function in substrate binding, phosphorylation and subcellular targeting properties...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25