some co orthologous cnes were duplicated in ancient events at the origin of vertebrates

Báo cáo y học: " The innate immune repertoire in Cnidaria - ancestral complexity and stochastic gene loss" pptx

Ngày tải lên : 14/08/2014, 20:22

... system, including the Toll/ TLR pathway and some complement cascade components, were in place at the base of the Eumetazoa Also represented in the datasets are proteins strongly matching both the ... 'IL-1R-type' In addition to the TIR domain, the first of these types contains a death domain and the second contains multiple LRRs Like the mammalian receptors for interleukin 1, the three Nematostella ... presence of ECSIT as well as the key components of the JNK/MAPK pathway in the cnidarian datasets (Table 1, Figure 4) indicates an early origin for this variant of Toll/TLR signaling The discovery of...

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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is speciﬁcally induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Ngày tải lên : 21/02/2014, 01:21

... The increase in expression levels of galA in the CREA mutant, indicates that at least one of the detected putative CREA binding sites in the promoter of galA is functional Hydrolysis of arabinogalactans ... starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-ﬂanking region, in pIM4700 (containing the pkiA promoter) A niger The sugar composition of the arabinogalactans ... production of high levels of proteins in A niger [33] The fusion construct (pIM3982) was made by introducing an NsiI site at the translation start point of galA and by then cloning a fragment, starting...

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Báo cáo khóa học: Identiﬁcation of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

Ngày tải lên : 16/03/2014, 16:20

... insulin by monitoring the kinetics of aggregation by light scattering As shown in Fig 7, curve 1, denatured insulin formed aggregates in the absence of Bt-Lon In contrast, at the : (w/w) ratio of ... dithiothreitolinduced aggregation of insulin B-chain to about 67% (Fig 7, curve 2) The result indicates that Bt-Lon is efﬁcient in preventing the aggregation of denatured insulin and in a dose-dependent ... To obtain an insight into the mechanism of thermostability of this protein, we compared sequences of thermophilic Bt-Lon with those of mesophilic Bs-Lon The G+C content of the protein-coding region...

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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

Ngày tải lên : 31/03/2014, 08:20

... concentration of 10% saturation, and the precipitate was removed by centrifugation Then, solid ammonium sulfate was added to the supernatant to give 80% saturation The resulting precipitate containing ... determined by detecting the formation of nitrite using the method of Ida et al [31] Km and Vmax of puriﬁed NaoA were determined from a double-reciprocal plot according to the LineweaverBurk equation ... for the formation of nitrite 0.175 lmolÆmin)1Ælg)1 according to the Lineweaver-Burk equation DISCUSSION We have cloned and determined the complete sequence of a gene encoding nitroalkane-oxidizing...

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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

Ngày tải lên : 31/03/2014, 09:20

... 8.0 containing enterokinase was added to the column which bind the fusion protein GSTBmKIM at 26 °C The eluate containing enterokinase was added again to the column The operation was repeated three ... or less The relative changes in the peak INa were plotted as a function of toxin concentration (Fig 4) The continuous line was drawn according to the equation: the percentage decrease in INa ¼ ... aconitineinduced arrhythmias Using the model of aconitine-induced arrhymia in rats and compared with distilled water, pretreatment of rBmKIM at 50 lgÆkg)1 signiﬁcantly increased the dosage of...

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Báo cáo hóa học: " Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains" potx

Ngày tải lên : 20/06/2014, 01:20

... are indicated by ●; the four mutations plus others at the primer binding site by ❍; three out of the four mutations by ■, and three mutations plus others at the primer binding site by ᮀ The open ... four mismatches found at the 5' end of the primer binding site Although two G1 strains correctly typed had a 100% identity with the untypeable strains, they concluded that the remainder 25% could ... this complex epidemiology [15] However, in recent years it was reported that the accumulation of point mutations in VP4 and VP7 genes was the main cause of the failure of the G or P-typing of rotavirus...

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Báo cáo y học: "Lack of association between mannose-binding lectin gene polymorphisms and juvenile idiopathic arthritis in a Han population from the Hubei province of China" docx

Ngày tải lên : 09/08/2014, 08:22

... Japan) PCR conditions were as follows: an initial denaturation at 94°C for minutes; 34 cycles of denaturation at 94°C for 30 seconds, annealing at 60°C for minute and extension at 72°C for minute; ... risk of the development of recurrent infection, in addition to systemic lupus erythematosus triggered by complement pathway deficiency states, indicates the importance of the complement system in ... in the protection against disease [5,17] Studies have shown that the MBL pathway is an independent pathway of complement activation [2] Mutation and deficiency of the MBL codon could impair the...

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Báo cáo y học: " Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand" ppsx

Ngày tải lên : 12/08/2014, 04:20

... of the NP gene (Table 4) On the other hand, the substitution rate of the NP gene in avian viruses was half (1.57 × 10-3) that of swine and human lineages, indicating the highly conserved nature ... acids of NP lineages identified in this study is involved in RNA binding activities [10] They are mainly correlated with host specificity of the viruses Genetic analysis of the NP gene of the 12 ... http://www.virologyj.com/content/7/1/185 human influenza viruses confirmed the highly conserved nature of the NP gene within host-specific lineages The NP gene of swine influenza viruses clustered with either...

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Báo cáo khoa học: " Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005" ppsx

Ngày tải lên : 12/08/2014, 04:21

... sequences and core data, contributed to the interpretation of the findings and revised the manuscript UB propagated the viruses, contributed to the interpretation of the findings and revised the manuscript ... implies that amino acids at the N-terminal RNA-binding domain of NS1 are implicated in this function The arginine at position 38 and the lysine at position 41 contribute to this interaction [10] The ... strain Fourty-one of these were transitions; 23 of these were pyrimidine and 18 were purine transitions Only of these substitutions resulted in amino acid changes in NS1 protein In the genome of...

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Báo cáo y học: "Nef gene evolution from a single transmitted strain in acute SIV infection" ppt

Ngày tải lên : 12/08/2014, 23:21

... modeling [1-3] Major goals of such analyses include the characterization of the transmitted strains, estimating the timing of infection based on the level of sequence diversity, and distinguishing ... histogram of the observed number of mutant sequences sampled in any of the sequential time points, Nm The 95% confidence intervals were calculated by repeating 102 of 102 MC runs The simulation confirmed ... larger the mutation rate, the faster the genomes mutate, hence the steeper the growth in diversity The greater the generation time, the slower the genomes diversify, hence the smaller the growth in...

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Báo cáo y học: " A method to estimate the efficiency of gene expression from an integrated retroviral vector" potx

Ngày tải lên : 13/08/2014, 09:20

... Estimating the proportion of populations that were resistant to puromycin Theoretically, after T days of culture in the presence of puromycin, the number of cells in the culture NT, the initial ... Estimating the proportion of cells that contained the provirus The proportion of cells that were successfully transduced could be estimated from the PCR data If the number of positive samples were ... negative control, amplifying water only The limit of detection was at lane with the DNA input of 25 fg, corresponding to an input of approximately 2300 copies of plasmid One potential source of...

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A Substance Isolated from Cornus officinalis Enhances the Motility of Human Sperm.pdf

Ngày tải lên : 16/12/2012, 15:24

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Tài liệu Retrieving a Single Value from a Query pdf

Ngày tải lên : 14/12/2013, 18:16

... The ExecuteScalar( ) method of the Command object returns a single value from the data source rather than a table or data stream While the ExecuteScalar( ) method does not result in a performance ... performance improvement when compared to retrieving a single value using an output parameter or using a DataReader, it allows a single value to be returned with the least code and may therefore improve ... maintainability If the result set returns more than one result, the first column of the first row is returned as a scalar value A null reference is returned if the result set is empty or if the...

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Tài liệu Pass a Dataset Back from an XML Web Service docx

Ngày tải lên : 24/12/2013, 06:17

... delete columns from a data table If the goal is to include all the columns in the table and accept the Password column, then when other columns are added, you will not have to touch the code because ... because the * is being used Tip When referring to tables on the receiving end, as displayed in step of this How-To, you have to refer to tables in the dataset by their ordinal values Therefore, some ... you created the Login windows Form Create a new Windows Form and place the controls shown in Table 13.2 in the order displayed in Figure 13.14 Table 13.2 Label, TextBox, DataGrid, and Command...

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Tài liệu Figure Drawing Without A Model - Drawing from Life pptx

Ngày tải lên : 21/01/2014, 08:20

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Tài liệu Getting a Sequence Value from Oracle pdf

Ngày tải lên : 21/01/2014, 11:20

... OracleConnection conn = new OracleConnection( ConfigurationSettings.AppSettings["Oracle_ConnectString"]); // Create the command for the insert stored procedure OracleCommand cmd = new OracleCommand( ); cmd.Connection ... and constants using System; using System.Configuration; using System.Windows.Forms; using System.Data; using System.Data.OracleClient; private const String STOREDPROCEDURENAME = "SP0404_INSERT"; ... name constants for table private const String ID_PARM = "pID"; private const String FIELD1_PARM = "pField1"; private const String FIELD2_PARM = "pField2"; // // Create the connection OracleConnection...

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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt

Ngày tải lên : 12/02/2014, 10:20

... containing 100àg/ml ampicillin, incubated in shakers at 37oC until the OD600 reached 0.6 Induction by IPTG Expression and purification of recombinant MCoTI-II were followed the manufactures instruction ... products of MCoTI-II gene Gctcgcggat ttatttcgag ttcagacctg ttcagacctg 40 41 ttattatggg tattacttta tctgatgatt ctgaTcatca 80 81 Gtttttgctt ggatcccagg ttgttgtaca gaatgctggt 120 121 catatgagcg gcagcgatgg ... ampicillin and IPTG concentrations, temperature and time of induction were tested The growth and induction conditions of PI-17 giving maximal yield of the recombinant fusion protein established as...

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Tài liệu Voices of Fear and Safety¿ Women¿s ambivalence towards breast cancer and breast health: a qualitative study from Jordan pdf

Ngày tải lên : 13/02/2014, 06:20

... [25] The PI condensed the Arabic text into meaning units followed by English coding and categorization The coding and categorization of the data was validated by the co researchers Thereafter the ... PI and the co researchers clustered the categories into emerging themes Triangulation of researchers was used to enhance the trustworthiness of the findings [26] Ethical considerations The ethical ... issued from the Jordan Ministry of Health Research Ethics Committee in 2009 The confidentiality and autonomy of the participants was insured They were informed of the purpose of the study, the voluntary...

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Breaking into the Game Industry: Advice for a Successful Career from Those Who Have Done It

Ngày tải lên : 13/02/2014, 17:23

... that the business of making games is a lot like the business of writing fiction: the more life experience a participant has, and the better they can communicate their ideas, the better off they’ll ... one of the top 20 most in uential women in the game industry by vi About the Authors Gamasutra.com in 2008 and one of the 100 most in uential women in the game industry by Next Generation magazine ... the professors are Some professors are out there in the industry, going to GDC, networking with and gaining respect in the game industry And remember that all schools will tell you that it matters...

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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

Ngày tải lên : 19/02/2014, 18:20

... terminus of the protein Using this method, we showed that asparagine 26 represents the amino terminus of P40 Knowing, that the ﬁrst amino acids of the processed P40 and P90 is the asparagine at ... experimentally whether there is a type I SPase activity in M pneumoniae, we determined the N-terminus of P40 as it appears in protein extracts of M pneumoniae Determination of the N-terminus of P40 by ... proteolytic contaminants of the trypsin being used like chymotrypsin or due to pseudotrypsin formed from trypsin by autolysis [28] These possibilities can be excluded, when the amino terminus of the intact...

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