solid liquid extraction chlorophyll from spinach leaves

Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf

Ngày tải lên : 22/02/2014, 04:20

... protein in spinach (A) Northern blot of total RNA from spinach leaves (B) Western blot of total protein extract of spinach leaves, and (C) SDS/ PAGE of lg of recombinant protein purified from S frugiperda ... RESULTS Isolation of cDNA for spinach leaf 6PF2K/Fru-2,6-P2ase A k phage cDNA library constructed from mature spinach leaves was screened with a 450-bp EST clone from Pinus taeda (partial sequence, ... has been purified from spinach leaves This activity is specific for hydrolysis of Fru-2,6-P2, and is inhibited by Fru-6-P and Pi, although the affinities for these inhibitors differ from those of the...

11
378
0

Membrane protected micro solid phase extraction of pharmaceuticals from environmental water samples

Ngày tải lên : 10/09/2015, 09:12

... Hollow fiber assisted liquid phase microextraction HILIC Hydrophilic interaction liquid chromatography HLB Hydrophilic-lipophilic balance LC Liquid chromatography LLE Liquid- liquid extraction LOD Limit ... conventional analytical extraction procedures such as SPE and classical liquid liquid extraction (LLE) Microextraction techniques are characterized by the extraction of analytes from a given donor ... Rr Relative recovery RSD Relative standard deviation SBME Solid bar microextraction SPE Solid phase extraction SPME Solid phase microextraction TEA Triethylamine TGA Thermogravimetric analysis...

90
323
0

Tài liệu LIQUID FOSSIL FUELS FROM PETROLEUM ppt

Ngày tải lên : 23/01/2014, 07:20

... 47.17 lists ultimate analyses of oils derived from shales from a number of locations.14 Properties will vary with the process used for extraction from the shale The objective of all such processes ... divide by 15.00 X sp gr, from column Calculated for boiling at midpoint of distillation range, from column ^Includes latent heat plus sensible heat of the vapor heated from boiling point to 60°F ... processing, and refining of oils from shale 47.4 OILS FROM TAR SANDS At the time that this is written, the only commercially practical operation for extracting oil from tar sands is at Athabaska,...

17
433
0

Báo cáo khoa học: Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts doc

Ngày tải lên : 08/03/2014, 08:20

... inhibition of the reaction catalyzed by spinach chloroplast DHAR We purified recombinant DHAR to homogeneity from E coli that expressed a cDNA for mature DHAR from spinach chloroplasts (Fig 1) Because ... and GSH, and kcat of the recombinant DHAR were the same as those of the DHAR purified from fresh leaves of spinach [15], we used the recombinant DHAR in this study DHAR catalyzes the reduction ... activity between spinach chloroplast DHAR and other DHA-reducing proteins, such as the thioltransferase from pig liver and trypanothione:glutathione disulfide thioltransferase from T cruzi, might...

8
354
0

Growth of amorphous silicon nanowires via a solid–liquid–solid mechanism

Ngày tải lên : 16/03/2014, 15:05

... of Si atoms from the substrate through the substrate liquid interface into the liquid droplets, and then through the liquid droplets–nanowire to stack themselves into nanowires? From a kinetic ... the Si–Ni eutectic liquid droplets; Žc continuous diffusion of Si atoms through the substrate liquid ŽSL interface into the liquid droplets, and growth of SiNWs through the liquid wire ŽLS interface; ... eutectic liquid alloy droplets Because of the relatively high solubility of Si in Si Ni eutectic alloy, more Si atoms will diffuse through the solid Žsubstrate liquid interface into the liquid...

5
503
2

Controlled growth of oriented amorphous silicon nanowires via a solid–liquid–solid (SLS) mechanism

Ngày tải lên : 16/03/2014, 15:14

... using Si substrate as Si source via a solid liquid solid mechanism by heating They have uniform length and diameter The growth is explained by solid liquid solid model This synthesis method of ... eutectic liquid alloy droplets Because of the relatively high solubility of Si in Si2 Ni eutectic alloy, more Si atoms will di use through the solid (the substrate) liquid interface into the liquid- phase ... compositional uctuations, resulting in the growth of SiNWs Because this growth process involves solid liquid solid phases, it is named as a SLS growth, which is in fact an analogy of the VLS mechanism...

5
386
0

Báo cáo khoa học: Surface density of cellobiohydrolase on crystalline celluloses A critical parameter to evaluate enzymatic kinetics at a solid–liquid interface ppt

Ngày tải lên : 23/03/2014, 10:21

... highly crystalline celluloses Highly crystalline celluloses, cellulose Ia from Cladophora and cellulose Ib from Halocynthia and from hydrothermally treated Cladophora, were characterized by transmission ... celluloses Bar indicates 500 nm (A) and (D) Cellulose Ia from Cladophora; B and E, cellulose Ib from Halocynthia; C and F, cellulose Ib from hydrothermally treated Cladophora Circles in the bottom ... FT-IR spectra of highly crystalline celluloses A, cellulose Ia from Cladophora; B cellulose Ib from Halocynthia; C, cellulose Ib from hydrothermally treated Cladophora Dotted line shows characteristic...

10
289
0

Báo cáo khoa học: Protein oligomerization induced by oleic acid at the solid–liquid interface – equine lysozyme cytotoxic complexes potx

Ngày tải lên : 30/03/2014, 01:20

... oleic acid was isolated and puriﬁed from the casein fraction of human milk [26,27] and denoted as multimeric a-lactalbumin (MAL), which indicates that the solid liquid interfaces of the chromatography ... share HAMLET-like and amyloid properties, may shed light on both phenomena The application of a solid liquid interface, facilitating protein self-assembly and protein–oleic acid interactions, proved ... ELOA is shown by a solid line of the free dye in solution is shown by a dashed line (B) Interaction of ELOA with ThT The ﬂuorescence spectrum of dye bound to ELOA is shown by a solid line and the...

15
319
0

Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

Ngày tải lên : 31/03/2014, 01:20

... DG, obtained from pressure-induced unfolding; DG*, obtained from GdmClinduced unfolding; TP1/2*, obtained from calculation for the net increase in free energy; TP1/2** obtained from calculation ... Curves from left to right: 0.0, 0.1, 0.2, 0.41, 0.83 and 1.47 M sucrose, respectively The unfolding degrees (a) were calculated from the ﬂuorescence spectra of the protein excited at 295 nm or from ... protein isolated from spinach photosystem II; the equilibrium is a two-state reversible transition which is inﬂuenced by NaCl [11] P1/2 (the halfpressure of denaturation) was shifted from 118 MPa...

8
527
0

liquid extraction

Ngày tải lên : 02/04/2014, 16:33

... distinct handicap operating against the choice of extraction There are other more subtle differences and even similarities between LIQUID EXTRACTION liquid extraction and other separation operations ... phases at LIQUID EQUILIBRIA B FIG 2.12 A Formation of Type system from two Type (a) FIG 2.13 17 systems with changing temperature (b) Formation of three liquid phases in equilibrium LIQUID EXTRACTION ... rule A in liquid extraction, and they will be described C FIG 2.16 Equilibria in a ternary system containing a solid component FIG 2.17 Distribution of a solid between two insoluble liquids Refer...

430
127
1

solid-liquid filtration and separation technology

Ngày tải lên : 02/04/2014, 16:35

... on the solid and liquid entering the filter system, Mass of solids in the filter cake: Mass of liquid in the filter cake: Mass fraction of solids in the slurry feed: Total mass of slurry (solid ... industry 8 I Solid Liquid Sepurution Technology Here, gravity sedimentation, involving a difference m density between solids and liquids, is used to produce an essentially clear overflow of liquid ... densities between the solid and the liquid The solid is allowed to sink m the &id, under controlled conditions In the reverse process of flotatiq the particles rise through the liquid, by virtue...

551
427
0

Báo cáo "A new 3α-acetoxy-urs-23,28,30-trioic acid from the leaves of Acanthopanax Trifoliatus " doc

Ngày tải lên : 03/04/2014, 15:20

... herbarium of the College of Pharmacy, Chungnam National University, Korea Extraction and isolation Dried and powdered leaves (3.7 kg) were extracted repeatedly with hot MeOH three times The combined ... chromatography on a silica gel column, using hexane-acetone (4 : 1) as eluent and repeatedly recrystallized from hexane-acetone (8 : 1) to yield (10.0 mg) as white crystals 3.4 -acetoxy-urs-23,28,30-trioic ... m/z: 561 [M+H]+; 1H- and 13C-NMR: see Table Acknowledgements: This study was supported by a grant from the Vietnam - Korea international cooperation project We are grateful to KBSI for measuring...

4
211
0

Báo cáo " Flavonoid glucosides from the leaves of Croton tonkinensis Gagnep., Euphorbiaceae " docx

Ngày tải lên : 03/04/2014, 15:20

... position, and the connection of the coumaroyl moiety to C-6” of glucose was judged from the HMBC correlation from the two proton signals at H 4.05 (strong) and 4.3 (weak) to the carbonyl carbon ... 1.5 Hz), 7.9 and 6.82 (both 2H, d, J = 8.7 Hz) supported by the correlated carbon signals derived from the HSQC spectrum and the 13C singlet signal at 177 in the 13C decoupled spectrum are indicative ... for a flavonol The molecular formula C30H26O13 was deduced from the quasimolecular ions 593 [M-H]+ (ESIMS negative-ion mode) and 617 [M+Na]+ (ESIMS positive-ion...

4
323
0

measurement of solid liquid interface temperature during pulsed excimer laser melting of polycrystalline silicon films

Ngày tải lên : 06/05/2014, 08:54

... laser ﬂuence The effect of a temperature gradient at the solid liquid interface is considered in calculating the interface temperature From numerical simulation,9 it is found that the temperature ... are in close agreement with those obtained from thermal emission measurement Considering that any possible thermal emission from the high-temperature solid silicon can last much longer than the ... emission from the top of the sample is also measured to verify that the surface temperature does not reach the boiling temperature ͑2628 K͒ In the case of surface evaporation, the solid liquid...

3
170
0

Báo cáo khoa học: "An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus" pdf

Ngày tải lên : 08/08/2014, 14:21

... kits and published methods for DNA extraction on the different trees we are working with, we developed a protocol to from mature leaves harvested in October, from four hardwood tree species: Quercus, ... provided DNA good quality by a quick method of extraction from tree species which have often been a problem regarding extraction of their DNA DNA yields from a 0.1gsample are sufficient for PCR and ... arboretum) and in some cases leaves from in vitro stocks were also used Fresh mature 2.2 DNA purification One hundred milligrams of fresh plant material (leaf) ground in liquid nitrogen using a ceramic...

5
389
0

Enrichment separation and determinations of cadmium(II) and lead(II) 1 phenyl 1h tetrazole 5 thiol chelates on diaion SP 207 by solid phase extraction flame atomic absorption spectrometry

Ngày tải lên : 02/09/2015, 13:45

... combination is not used for the solid phase extraction studies In this work, a simple and accurate procedure for cadmium(II) and lead(II) ions that is based on solid phase extraction of cadmium(II) ... plasma optical emission spectrometry; CoP: Coprecipitation; CPE: Cloud point extraction; DLLME: Dispersive liquid liquid microextraction; MF: Membrane ﬁltration; LOD: Limit of detection samples The ... F., 2010 Ionic liquid- based dispersive liquid- liquid microextraction and enhanced spectrophotometric determination of molybdenum (VI) in water and plant leaves samples by FOLADS Food Chem Toxicol...

6
406
0

The application of silica monolith for solid phase extraction

Ngày tải lên : 10/09/2015, 15:50

... silica monolith as solid phase extraction cartridge for the extraction of epinephrine, metanephrine and normetanephrine from urine  Application of the silica monolith as solid phase extraction cartridge ... cartridge for the extraction of ketamine from urine  Application of the silica monolith as solid phase extraction cartridge for the extraction of morphine, codeine and cocaine from urine  Application ... silica-based monolith as solid phase extraction cartridge for extracting polar compounds from urine Talanta 82:488-494 • Nema T, Chan ECY, Ho PC 2011 Extraction of ketamine from urine using a miniature...

155
551
0

Equilibrium and kinetic studies on the liquid liquid extraction and stripping of l phenylalanine via reversed micelles

Ngày tải lên : 05/10/2015, 21:28

... Reversed Micelles in Liquid- Liquid Extraction 2.6 Equilibrium Studies on Liquid- Liquid Extraction of Amino Acids using Reversed Micelles 2.7 2.8 13 Kinetics Studies on Liquid- Liquid Extraction and ... 2.1 Literature Review Typical Liquid- Liquid Extraction and Stripping Processes Separation by liquid- liquid extraction involves the transfer of the desired solutes from one phase to another The ... the liquid- liquid re -extraction of amino acids from the micellar phase to the strip aqueous 18 solution It is therefore interesting to investigate if the parameters studied in the liquidliquid extraction...

247
487
0

DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf

Ngày tải lên : 22/03/2014, 14:20

... surrogates are extracted from a water sample by passing L of sample water through a cartridge or disk containing a solid matrix with a chemically bonded C18 organic phase (liquid -solid extraction, LSE) ... collect samples from the flowing stream Keep samples sealed from collection time until analysis When sampling from an open body of water, fill the sample container with water from a representative ... spectrometer Table lists accuracy and precision data from replicate determinations of method analytes in tap water using liquid -solid cartridge extractions and the ion trap mass spectrometer Any...

60
528
0

extraction of liquid crystals from flat panel display devices using both liquid and supercritical carbon dioxide

Ngày tải lên : 28/04/2014, 10:50

... dispersed liquid crystal devices.[6,7] Their work demonstrated the potential to fractionate commercially available liquid crystal mixtures Following from this the first carbon dioxide extractions ... dioxide extraction was carried out using a Thar Technologies SFE-500F-2-FMC System and liquid withdrawal carbon dioxide (99.9 %) The extraction vessel was charged with 300 g of glass from a LCD ... conditions of 250 bar and 40ºC the extraction recovery from complete screens was poor, 0.1% of the total liquid crystal present The removal of a resin seal from the outer edge of the LCD did...

6
308
0