smoking depression and the hpa axis
báo cáo khoa học: " The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro" ppsx
... of the study, organized and carried out the PCR studies, designed the primers and drafted the manuscript CK carried out the PCR studies as well TZ participated in the design of the study RS and ... curve at the exponential part This method was applied to calculate the cycle number and CT-value for quantitation Furthermore, the CT-values of actin, GAPDH and RPS18 housekeeping genes and the individual ... participated in the study design, supported by scientific consulting and coordination and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements Thanks to the...
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DEVELOPMENTAL DIFFERENCES AND ALTERED GENE EXPRESSION IN THE TS65DN MOUSE MODEL OF DOWN SYNDROME
... for scientists 1.8 Thesis Hypothesis Since the PA1, or mandibular precursor, is significantly smaller at E9.5 and the mandible is reduced at P0, it is hypothesized that the mandibular precursor ... microwave and then immediately poured into a glass Coplin jar The Coplin jar was then placed into the 100°C water bath and the slides were then placed in to the Coplin jar for 20 minutes The slides ... After the two hour blocking period, the donkey serum was removed and the diluted Sox9 antibody was pipetted onto the slide The slides were then incubated overnight at 4°C 27 The following day, the...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... [12,41] Therefore, MLL1 and H3K4 trimethylation have been shown (by our laboratory and others) to be colocalized in the euchromatic regions of the nucleus, and this is probably because of their ... DAPI to visualize the DNA Merge shows the merge between DAPI and MLL1 images Merge shows the merge between DAPI and differential interference contrast images of the same cell Late the beginning of ... cyclins E and B, as a function of the cell cycle Western blot analysis of the whole-cell extract and histones from different stages of the cell cycle demonstrated that the overall levels of MLL1 and...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
... at 59°C for 35 s and extension at 72°C for min, and followed by the final extension at 72°C for 10 The primers were shown in Table and Table Materials and methods Cell culture and transfection ... and 38.04%, respectively Furthermore, the relative mRNA expression level of PTEN was higher than other genes Except for FHIT and CHFR, the mRNA expression levels of CCNA1, PAX1, PTEN, SFRP4 and ... cells These researches demonstrated us that FHIT and PTEN in Hela cells and FHIT and CHFR in Siha cells might have the other regulation pathways for carcinogenesis or transcription control, and...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... remains unclear [38] Due to the fact that HPAIV and LPAIV infection can cause a different degree of immune response, we hypothesized that the highly pathogenic properties of HPAIV may be caused by ... over the last 10 years, and there is evidence that it is more virulent in humans [46-48], we chose H1N1 because of its lower pathogenicity and therefore a better reference for comparison with the ... cDNA using the poly(dT) primers and Superscript III reverse transcriptase (Invitrogen), and quantified by real-time PCR The sense and antisense primers used in real-time PCR for measuring the cytokines/chemokines...
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Báo cáo y học: "Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes" docx
... applied for the PCR test The PCR was done as described in Materials and Methods After the reaction, the amplified product was taken out of the tubes and run on 2% agarose gel (A) and (B): Lane ... (Lane 6), DMSO and anti-CD3/CD28 Ab (Lane 7), and AC and anti-CD3/CD28 Ab (Lanes to 10) respectively (Figure 4A and 4B) The results indicated that the levels of IL-2 (P = 0.003) and IFN-g (P = ... (luc2P/NFAT-RE/Hygro), were cultured in the presence of AC (6.25, 12.5 and 25 μM) for four hours The cellular proteins were then extracted from the cells and subjected to the luciferase activity assay...
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Báo cáo y học: " Gene therapy in animal models of rheumatoid arthritis: are we ready for the patients" pdf
... apoptosis inducing ligand (TRAIL) or Fas ligand (FasL; see Genetic synovectomy, below) [25,26] The DCs not migrate to the joint, and exert their effects in the spleen and lymph nodes The most sophisticated ... Ideally, the gene therapeutic approach of cytokine modulation must parallel the intermittent course of the disease, so that the variable physiological demands may be met and undesirable exposure to the ... around the fact that the rheumatoid factors in RA patients may form immune complexes with the immunoglobulin domain of the tgAAC94 fusion protein, thereby inactivating the therapeutic protein in the...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
... plotting the specific binding of the radioligand on X -axis and bound/free on Y -axis using Sigma plot software (version 2.0, Jandel GmbH, Erkrath, Germany) The maximal binding is a measure of the total ... total number of receptors present in the tissue and the equilibrium dissociation constant is the measure of the affinity of the receptors for the radioligand The Kd is inversely related to receptor ... primer and TaqMan probe for assay on demand and endogenous control β-actin and 12.5 μl of Taqman 2× Universal PCR Master Mix (Applied Biosystems) and the volume was made up with RNAse free water The...
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Báo cáo khoa học: "Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats" doc
... of the HF and the NF diet fed groups (p < 0.01, Figs 11 and 12) NPY immunoreactivity of the ARC and the SCN was denser in the HF than in the same areas of the NF diet fed groups (Fig 13) In the ... observed in the LHA, and they were 13 to 30 µm in size and multipolar to fusiform in shape (Fig 11) On the other hand, the NPY-IR cells were to 10 µm in size and mainly oval in shape in the ARC (Fig ... in the SCN of the HF and NF diet fed groups **; p
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
... expression of the apoptosis inducing Fas and Fas ligand genes [10], whereas the degree of apoptosis was reduced in the focal infiltrates and unchanged in the glandular epithelial cells [43,44] The balance ... that the loci affect the gene expression profiles in the SGs of the congenic mice The gene expression reflected the congenic fragments When comparing the gene expression of the B10.Q.Nss1 and the ... and data analysis AKL, RH, AJ and MJ participated in the breeding of the mice and collected the organs KP and AKS participated in the microarray data analysis AKL, RH and RJ participated in the...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... unclear whether this is the case in the IVD and how important TNF-α is to human IVD degeneration One possibility is that TNF-α upregulates IL-1β, which then upregulates itself and IL-1α and further ... of the experimental work, supervised PWC, analysed the data and drafted the manuscript PWC carried out the remainder of the experimental work AJF helped to secure funding and contributed to the ... preparation of the final manuscript JAH conceived and designed the study, secured funding and co-wrote the manuscript All authors read and approved the final manuscript Acknowledgements 17 18 19 20 The...
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Báo cáo y học: "Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver" ppt
... vehicle and parthenolide control animals (Fig 5) The level of IL-1β mRNA in spleen and liver of vehicle and parthenolide control animals was negligible In the spleens of co-treated animals, there ... and as a result, express proinflammatory cytokines to a greater extent [21] These and other cell types including endothelial and epithelial cells might also contribute differences in spleen and ... provided standard rodent chow and water ad libitum, and acclimated to their environment at least one week before the start of experiments Chow and water were removed from cages one hour prior to the...
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Báo cáo y học: "Response of the mouse lung transcriptome to welding fume: effects of stainless and mild steel fumes on lung gene expression in A/J and C57BL/6J mice" pot
... interests The authors declare that they have no competing interests Authors' contributions PCZE performed the animal exposures, isolated the RNA, ran the arrays and the IPA analysis and drafted the ... A/J and B6 mice (Table and 4) However, as previ- ously mentioned, the gene networks, numbers and regulation contrasted between the strains The diseases and disorders that differed between the ... diseases and disorders, molecular and cellular functions and physiological system development and functions for A/J and B6 mice and 16 weeks post-exposure to GMA-MS and -SS welding fume The range...
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Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt
... previously observed in the two other major orders where haemochorial placentae have emerged: the primates (syncytin-1 and -2) and the rodents (syncytin-A and -B) It is therefore likely that such ... classically observed for retroviruses Their position and functionality ¢ Figure the the human identified syncytins tions of and mouserabbit Env-Ory1- and Env-Ory2, and of Retroviral envelope protein-based ... protein-based phylogenic tree with positions of the identified rabbit Env-Ory1- and Env-Ory2, and of the human and mouse syncytins The tree was determined by the neighbor-joining method using envelope...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... synchrony The data should speak for themselves 7) The DNA distribution of cells should be narrow in the synchronized cells and these distributions should then reflect the movement of cells through the ... synchronized The DNA patterns start with an 8N (it appears) value for DNA content, which goes down to 2N and back to 4N and never repeats the 8N In between the start and the end there is no systematic ... the comparison of the two experiments, I present a graph (Figure 1) of the K values in a scatter diagram The R2 value is 0.37, which some may say is "rather strong" and others may say is "rather...
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Báo cáo y học: "Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants" pptx
... examine the regulation of these funcats in all of the sub-models This analysis enabled us to determine into which sub-models the genes from these funcats fell and to determine whether the genes in these ... were used as the parent list, n was the number of genes in Model CN-enhanced (the child list), k was the number of genes in the pathway being analyzed and in the child list, and p was the number ... expected in the promoter, the sequence from RSA tools [39,40] was randomized 5,000 times and the above procedure was repeated The number of times CN1 and/ or CN2 were found in the randomized versions...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... tracer, and the cDNA population of lymphoma h2 as driver; and vice versa, 2) the cDNA population of lymphoma h2 as tracer, and the cDNA population of lymphoma h1 as driver The cDNAs selected by the ... oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit (capn4) and other (in case of lymphoma h1) and SMG-1, the gene ... another factors, such as different proliferation rates of the cell populations examined Another open question is whether there are differences in the expression of genes in HIV/SIV-related and...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... influence on the behaviour of the cells, which may alter the biochemical effect of the transported cargo and which could consequently influence the interpretation of the results Table List of the examined ... examined transport peptides The table displays the amino acid sequences of the investigated transport peptides (TP) and transporter molecules used in the study The synthesis and application are described ... Comparison of the expression level of the reference genes The main modified genes (grey in table 2) and the reference genes used are listed The graph displays the relative expression level of the used...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... treatment, neither treatment with the E-cad2 antibody nor the EGFR antibody induced Erk activation (Fig 2E) Taken together, these results suggest that the observed Erk activation was specific for the disruption ... [49], and a CHO-derived cell line [50] However, the site at which the PI3K signaling feeds into the Erk activating pathway varies in these systems: at the Ras, Raf or MEK activation level In the ... adhesion and induces uPA gene expression Under normal growth conditions, T47D and MCF7 breast cancer epithelial cell lines grow very compact and E-cadherin was concentrated at the border of the cell–cell...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... assess the implication of the Ras/Raf pathway, SMC were cotransfected with the )973 bp cyclin D1 gene promoter and either the dominant-positive or the dominant-negative Ras and Raf mutants, and the ... anti-(cyclin D1) Ig (lower part) and the quality of the preparation and the amount of protein loaded were evaluated with anti-actin Ig (upper part) (D) The gel was blotted and incubated with specific ... with these constructs and then stimulated with IL-1b The ()137 to )99 bp) Wt Egr-1 construct was regulated by PUFAs in the same manner as the ()973 to +29 bp) cyclin D1 gene promoter (Fig 4C) The...
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