sites of disruption of reproductive function
Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx
... of NAD+ to NADH by reductive inhibition of epimerase offered a sensitive method for determination of stoichiometry of the bound cofactor Reductive inhibition was applied to 33.3 nmol (5 mg) of ... uncertainty of protein concentration determination and the incompleteness of conversion of NAD+ to NADH Therefore, with respect to the composition of cofactor, the catalytic sites of epimerase ... formation of E3 As E2 has no additional 5¢-UMP-binding site, its conversion to E3 should be independent of added 5¢-UMP A corollary of the prediction is that half of the catalytic sites of E1 and...
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Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
... Ca2+-binding sites of TG2 ´ly R Kira et al Fig Multiple sequence alignment of Ca2+-binding sites of transglutaminases Sequence alignments of Ca2+-binding sites of TG2 compared with the other members of ... confirmed that each of the five mutagenized sites contributes to Ca2+ binding of TG2 It was also observed that disruption of one site by mutation leads to weaker ⁄ loss of binding to other sites, and this ... site of wild typeTG2 0.0 Weak binding sites of wild type TG2 Weak binding sites of S1 TG2 0.0 0.00 –0.1 –0.3 n = 0.52 ± 0.63 Kd = 0.1 ± 0.03 µM –0.4 kcal·mol–1 of injectant –0.2 kcal·mol–1 of injectant...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... In 20 lL of reaction mixture, MeMIH-B DNA template was mixed with appropriate amounts of nuclease-free water, lL of 10 · T7 reaction buffer, lL of ATP solution, lL of CTP solution, lL of GTP solution, ... neuropeptide gene, and use of an in vitro explant culture system and an RNAi technique to demonstrate the reproductive function of MeMIH-B in M ensis Results Expression of MeMIH-B in shrimp Although ... injection of an equal amount (6.6 nmol) of rMeMIH-A did not cause any change in the overall expression of vitellogenin in the hepatopancreas and ovary (Fig 3A,B) In contrast, injection of 6.6 nmol of...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... contribution of spontaneous NADPH oxidation and other reductases in the samples can be ignored One unit of GR activity was defined as the amount of enzyme that catalyzes the oxidation of lmol of NADPH ... RESULTS Levels of GR in male reproductive system of the adult rat To identify the cells responsible for GSSG reduction, an attempt was made to determine the localization of GR in the male reproductive ... different turnover rates of the protein in these tissues Immunohistochemical localization of GR in male reproductive system of the adult rat We employed immunohistochemical analyses of GR in order to...
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Báo cáo y học: "Effect of interleukin-1β on spinal cord nociceptive transmission of normal and monoarthritic rats after disruption of glial function" pot
... excitatory effect of IL-1β on spinal cord wind-up, irrespective of the normal or monoarthritic condition of rats The results suggest that the excitatory effect of nanomolar doses of IL-1β Page of (page ... administration of a single dose of ng of IL-1β to the NS group resulted in about 80% increase of wind-up activity 20 minutes after the injection (Figure 3b, P < 0.05) In contrast, ng of IL-1β intrathecally ... Zhang YQ: Disruption of glial function enhances electroacupuncture analgesia in arthritic rats Exp Neurol 2006, 198:294-302 Watkins LR, Maier SF: Immune regulation of central nervous system functions:...
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handbook of fermented functional foods
... Technologies (2007) Dictionary of Nutraceuticals and Functional Foods (2006) Handbook of Functional Lipids (2006) Handbook of Functional Dairy Products (2004) Handbook of Fermented Functional Foods, Second ... Methods of Analysis for Functional Foods and Nutraceuticals (1st and 2nd Editions), Handbook of Fermented Functional Foods (1st Edition), Handbook of Functional Dairy Products, Handbook of Functional ... Publication of book Prolongation of Life by Eli Metchnikoff describing therapeutic benefits of fermented milks Application of microbiology to fermentation, use of defined cultures Development of products...
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Tài liệu Activity 12.1: Risks of No Functional Specification doc
... folding are documented in the functional specification After completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS ... 104 Activity 12.1: Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct...
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Tài liệu lackwell Publishing, Ltd. Predictors of reproductive cost in female Soay sheep pptx
... of Table can be simplified and/or specific effects re-tested in a more parsimonious environment Moreover the reproductive trade-off function could be further modelled using a specific function of ... variation in the trade-off function in a capture–recapture framework we have extended their results showing: (i) that the cost of reproduction varies as a quadratic function of the age of the mother, ... onset of reproductive senescence in domestic sheep may be because of a trade-off between breeding events and litter size An age-dependent cost of reproduction could also be a characteristic of other...
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Tài liệu Improving the quality of reproductive health care for young people doc
... 1999: 18) Lack of Information Young people may be unaware of their risk of pregnancy, unfamiliar with STI symptoms, and unsure where to obtain services and what types of services are offered A case ... Χαρε Address the Full Range of Young People’s Reproductive Health Care Needs Health care facilities need be aware of and address the full spectrum of young people’s reproductive health care needs ... Ashford, Yvette Collymore, and Nancy Yinger of PRB; Michal Avni, Sarah Harbison, Rachel Lucas, and Kellie Stewart of the USAID Office of Population and Reproductive Health, Bureau for Global Health;...
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Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... sites, one for ATP, one for ADP and one for BPG [5,12] On the basis of the results obtained from crystallization of a truncated form of cN-II [11], we constructed point mutants for a number of ... disappearance of the heavier inactive peak Furthermore, FPLC analysis of the recombinant wild-type cN-II showed the presence of the tetrameric active form irrespective of the presence of effectors ... an alteration of this ratio for a mutant was considered as caused either by an alteration of the Km value for one of the two substrates or by a variation of the kcat value for one of the two activities...
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Tài liệu Reserve Bank of India: Functions and Working doc
... Government of India managed the issue of paper currency In 1935, when the Reserve Bank began operations, it took over the function of note issue from the Office of the Controller of Currency, ... reduce the risk of counterfeiting or forgery of currency notes The Paper Currency Act of 1861 conferred upon the Government of India the monopoly of note issues, thus ending the practice of private ... 26 Issuer of Currency Management of currency is one of the core central banking functions of the Reserve Bank for which it derives the necessary statutory powers from Section 22 of the RBI Act,...
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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc
... structure and function features of the various groups of phenolics are sketched below The structures of a variety of simple and more complex polycyclic phenolics are presented in the order of increasing ... understanding the nature and function of the targets of plant defensive metabolites and proteins The remainder of the book summarizes (mainly in table form) a wealth of information Plant defensive ... kill plant-consuming organisms 1.3 Description of the tables Most of the book is comprised of tables dedicated to specific targets or groups of targets of plant defensive compounds Target-related...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... formation of supercomplexes and the effect of the absence of intact complex I, but the results are too preliminary in this regard Heterologous expression of the ESSS-HA subunit The localization of the ... EcoRI and NheI sites for cloning into the unique EcoRI and NheI sites of the modified pTRIDENT-14neo vectors such that either the HA or the HIS epitope tag was added to the end of the ESSS ORF ... incorporation of the tagged ESSS subunit into a functional complex I (Fig 3B, right panel) The signal from the 18 kDa subunit of complex I (NDUFB6) served as another identification of the complex...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... The mass spectrometry of radioactive peptides showed the presence of a doubly charged phosphopeptide of mass 974.0 and a singly charged phosphopeptide of mass 1946.3 both of which correspond only ... is the kinase Fig Summary of phosphorylation sites on recombinant syntaxin-1A The figure shows the sites of phosphorylation by CKIa (Thr21) and CKII (Ser14) The masses of the major peptides, in ... (absorbance at 210 nm) with positions of 32 P-labelled tryptic peptides of syntaxin-1A (1–190) phosphorylated by CKIa A C-terminal truncated construct of mutant of syntaxin-1A containing residues...
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Bariatric Surgery in Women of Reproductive Age: Special Concerns for Pregnancy pot
... evidence of hormone normalization, there is also indirect evidence of normalization through the return of normal menstrual cycles in women who had had irregular menses Of the 40 percent of women of reproductive ... College of Obstetricians and Gynecologists (ACOG) nominated the topic of this report and provided the following initial list of questions: What is the incidence of bariatric surgery in women of reproductive ... bariatric surgery in women of reproductive age? What are the trends in incidence of bariatric surgery in women of reproductive age? At least 50,000 women between the ages of 18 and 45 undergo bariatric...
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COMPONENTS OF REPRODUCTIVE ISOLATION BETWEEN THE MONKEYFLOWERS MIMULUS LEWISII AND M. CARDINALIS (PHRYMACEAE) potx
... series of sequential stages of reproductive isolation, a reproductive barrier can only prevent gene flow that was not already eliminated by previous stages of isolation (eq 4) Hence, components of reproductive ... 1971) Total Reproductive Isolation We compute total (cumulative) reproductive isolation between M lewisii and M cardinalis as a multiplicative function of the individual components of reproductive ... Because of the labor required to estimate the relative frequency of F1 individuals in the progeny of mixed pollinations, we studied the progeny of each cross type from eight of the 17 seed parents of...
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THE JOHNS HOPKINS HOSPITAL DIVISION OF REPRODUCTIVE ENDOCRINOLOGY doc
... check ( ) any of the following methods of contraception you are currently using and/or have use in the past Fill in the dates of usage Methods ( ) Birth Control Pills ( ) IUD Dates of Usage Name: ... dates of your last two menstrual periods? At what age did you begin to menstruate? What is the average length of your menstrual cycle? (Interval from 1st day of period ... any other type of allergies? _ _ List current medications (include the name of medication and duration of use) Medication:...
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THE RAPID EVOLUTION OF REPRODUCTIVE PROTEINS pptx
... from other species The study of the mechanisms of speciation is one of the central areas of interest in evolutionary biology Although the role of rapid evolution of reproductive proteins in the ... priori knowledge of the sites under selection and can be used to predict the functionally important sites of a gene that are subject to positive Darwinian selection10 A signal of positive Darwinian ... empirical data show the generality of the phenomenon of rapidly evolving reproductive proteins But what is the theoretical outcome of the continual coevolution of pairs of gamete-recognition proteins?...
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Đề tài " Norm preserving extensions of holomorphic functions from subvarieties of the bidisk " potx
... Thomas devised an elegant function theoretic proof of Theorem 1.19 We include his proof in an appendix at the end of the paper 295 HOLOMORPHIC FUNCTIONS The equivalence of the von Neumann inequality ... that (5.19) holds, then λ is in a subset of V which is an extremal disk of type This completes the proof of Lemma 5.17 In light of Lemma 5.17 the proof of Theorem 1.20 will be complete once we ... the proof of Lemma 5.1 Before continuing, we remark that an alternate proof of Lemma 5.1 can be obtained by showing that the Shilov boundary of P (V − ) must necessarily lie in the boundary of D2...
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Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx
... catalytic domain of the enzyme The role of the particular oxygen atoms of the selected phosphate groups is also discussed Moreover, we analyzed the function of the oxygen moiety at C6 of nucleoside ... Comparison of the Mg2+-dependent activity of the unmodified deoxyribozyme 10–23 with that of thio-substituted deoxyribozyme RP-P5 in the presence of mM MgCl2 Time course of cleavage reaction of a chimeric ... ratio of kMg of obs the reference unmodified enzyme to kMg of the particular obs FEBS Journal 274 (2007) 1062–1072 ª 2007 Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences...
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