site directed disulfide cross linking to probe conformational changes of a transporter during its functional cycle
Báo cáo Y học: Contribution of Lys276 to the conformational flexibility of the active site of glutamate decarboxylase from Escherichia coli pptx
Ngày tải lên :
31/03/2014, 08:20
... lysine mutants of aspartate aminotransferase [7], tryptophan synthase [4], D-amino acid transaminase [5] and alanine racemase [27] it was observed that exogenous amines can partially or totally substitute ... in GadB-K276H mutant, and therefore the data are omitted Addition of 20 mM glutamate to GadB-K27 6A produced an increase in absorbance at 412 nm and a decrease at 328 nm each with the same half-time ... with lysine and the cofactor aldehyde For example, aspartate aminotransferase is converted from an inactive 430 nm-absorbing protonated internal aldimine to an active unprotonated 362 nmabsorbing...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
Ngày tải lên :
06/03/2014, 00:20
... related to partial catalytic activation following (Mg)ATP binding at physiological concentrations of Glc Similar or possibly larger effects of ATP in promoting a catalytically competent state may ... Mutants of glucokinase cause hypoglycaemia- and hyperglycaemia syndromes and their analysis illuminates fundamental quantitative concepts of glucose homeostasis Diabetologia 42, 1175–1186 21 Mahalingam ... atom-positional backbone rmsd of the MD trajectory structures during the 2-ns MD simulations, relative to the starting structures, and calculated B-factor values based on fluctuations of Ca carbons...
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Báo cáo khoa học: A 49 kDa microtubule cross-linking protein from Artemia franciscana is a coenzyme A-transferase pdf
Ngày tải lên :
30/03/2014, 20:20
... Constant, QC, Canada) and cared for in accordance with guidelines in ÔGuide to the Care and Use of Experimental AnimalsÕ available from the Canadian Council on Animal Care The blots were washed ... in rabbits included an anti-peptide antibody to the N-terminal 15 residues of p49 [19] and an antibody prepared to native p49 during this study Rabbits were obtained from Charles River Canada (St ... demonstrated p49 is a CoA-transferase Fig Coassembly of Drosophila tubulin and the p49 analogue Tubulin in Artemia and Drosophila cell-free extracts was assembled by the addition of taxol and GTP...
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Tài liệu HOW TO MEASURE THE IMPACT OF A CRM STRATEGY ON THE FIRM PERFORMANCE doc
Ngày tải lên :
20/12/2013, 17:15
... non-financial performance measures such as customer and employee satisfaction, customer and employee retention and quality measures and financial indicators such as profitability (Banker and Mashruwala, ... Research, 10, 205-38 Ittner, C.D and D.F Larcker (1998b), ``Are non-financial measures leading indicators of financial performance? An analysis of customer satisfaction’’, Journal of Management Accounting ... (Amir and Lev, 1996; Srivastava, Shervani and Fahey, 1998) and profitability was proved, and subsequently this type of measures started to have a great deployment Nowadays the increasing dynamic...
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Tài liệu Quantification of the Health Effects of Exposure to Air Pollution: Report of a WHO Working Group pdf
Ngày tải lên :
17/02/2014, 11:20
... life-table calculation of survival probabilities takes into account that deaths take place throughout a year Without precise dates of each death, the usual (“actuarial”) convention is that about half ... COMMITTEE OF THE ENVIRONMENTAL AND OCCUPATIONAL HEALTH ASSEMBLY OF THE AMERICAN THORATIC SOCIETY (ATS) Health effects of outdoor air pollution, Part American journal of respiratory and critical care ... COMMITTEE OF THE ENVIRONMENTAL AND OCCUPATIONAL HEALTH ASSEMBLY OF THE AMERICAN THORATIC SOCIETY (ATS) Health effects of outdoor air pollution, Part American journal of respiratory and critical care...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
Ngày tải lên :
21/02/2014, 01:21
... presence of ADP or ATP can only be a consequence of conformational changes caused by the binding of the nucleotide to the enzyme active site region Binding of CO2 (expressed as total bicarbonate), another ... cation, a negligible enhancement of uorescence was obtained when OAA was added in the presence of Mg2+ or in the absence of divalent cations The OAA decarboxylation to pyruvate was lower than ... P-pyridoxyl adduct of PEP carboxykinase exhibits a band with a maximum at 326 nm due to pyridoxyl moiety and a band at 280 nm corresponding to the aromatic amino acids of the protein The uorescence of...
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Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study docx
Ngày tải lên :
07/03/2014, 15:20
... preparation Bovine bLG (AB mixture), chromatographically purified and lyophilized to ‡ 90% purity (Sigma; catalogue no L-3908), N-acetyltryptophanamide (NATA) (Sigma; catalogue no A- 6501) and AOT ... basis Data analysis was performed by a deconvolution method using a nonlinear least-squares fit programme, based on the Marquardt algorithm The goodness of fit was evaluated by statistical parameters ... a measure of the proximity of the quencher molecule to the fluorophore The calculated values are ˚ almost within the van der Waals contact (6–7 A) at x0 10 Increasing x0 leads to an increase of...
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Báo cáo khoa học: Conformational studies of a hyperthermostable enzyme potx
Ngày tải lên :
07/03/2014, 21:20
... single-domain protein with a molar mass of 30 085 Da Experimental data from mass spectroscopy (MALDI TOF) and size exclusion chromatography showed that LamA in solution is a monomer LamA contains ... spectroscopic analysis will enable us to characterize the thermally and chemically denatured states of LamA Using a combination of circular dichroism, steady-state and time-resolved spectroscopy and anisotropy ... purification was achieved by size exclusion chromatography in a Superdex 200 column (Amersham Pharmacia, Piscataway, NJ, USA) Pure LamA was stored at °C in 0.01 m sodium phosphate buffer at pH...
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Ten Ways to Improve the Security of a New Computer doc
Ngày tải lên :
14/03/2014, 22:20
... and install all available updates Enable automatic updates if your vendors offer it; that will ensure your software is always updated, and you won’t have to remember to it yourself Many operating ... Software Installing an antivirus and antispyware software program and keeping it up to date is a critical step in protecting your computer Many types of antivirus and antispyware software can detect ... daily, and vendors frequently make new signatures available, so antivirus software will be most effective if the signatures are up to date Many antivirus and antispyware programs offer automatic...
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Ocean Acidification: A National Strategy to Meet the Challenges of a Changing Ocean docx
Ngày tải lên :
15/03/2014, 15:20
... surface waters has already been observed in the Canada Basin of the Arctic Ocean (Bates et al., 2009; Yamamoto-Kawai et al., 2009) Persistent undersaturation of surface waters with respect to aragonite ... climate service and marine ecosystem management programs to develop a broad strategy for decision support DATA MANAGEMENT Data quality and access, as well as appropriate standards for data reporting ... management offices and databases that could support ocean acidification observational and research data The FOARAM Act also calls for an “Ocean Acidification Information Exchange” that would go...
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Báo cáo Y học: The unorthodox histidine kinases BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier ppt
Ngày tải lên :
17/03/2014, 23:20
... domain of the phosphorelay histidine kinase A of Bacillus subtilis was recently shown to be a catalytic ATP-binding domain [31] As the PAS domain of BvgS contains a putative ATP binding site and ... Bordetella pertussis BvgAS signal transduction cascade Proc Natl Acad Sci USA 91, 1163–1167 Utsumi, R., Katayama, S., Taniguchi, M., Horie, T., Ikeda, M., Igaki, S., Nakagawa, H., Miwa, A & Noda, M ... example, it is known that high aeration and logarithmic growth of the bacteria leads to a predominance of Q-8, whereas under anaerobic conditions and in stationary phase MK-8 is the predominant...
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facile hydrothermal route to the controlled synthesis of a - fe2o3 1 - d nanostructures
Ngày tải lên :
19/03/2014, 16:48
... occurred immediately Then the mixture solution was transferred into a commercial stainless steel Tef- lon-lined autoclave of 50 mL capacity The autoclave was maintained at a temperature of 180°C for ... HRTEM image and the SAED pattern may also be indexed to hexagonal phase of α-Fe2O3 The observed lattice spacings of 0⋅370 and 0⋅269 nm correspond to the (012) and (104) planes of hexagonal α-Fe2O3, ... 2006 J Am Chem Soc 128 5468 923 Kotsikau D, Ivanovskaya M, Orlik D and Falasconi M 2004 Sensor Actuat B101 199 Rumyantseva M et al 2006 Sensor Actuat B118 208 Sorescu M, Diamandescu L, Tarabasanu-Mihaila...
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Báo cáo khoa học: Inhibitor-mediated stabilization of the conformational structure of a histone deacetylase-like amidohydrolase pptx
Ngày tải lên :
30/03/2014, 08:20
... 258–270 Inhibitor-mediated stabilization of HDAH 31 Ray S, Bhattacharyya M & Chakrabarti A (2005) Conformational study of spectrin in presence of submolar concentrations of denaturants J Fluoresc ... Eqns 5, and 9) KC, ratio of off-pathway intermediate C and denatured state D; kf, the folding rate in the absence of denaturant; KI, ratio of on-pathway intermediate I and denatured state D; mf ... impact of zinc ions, and, to a lesser extent of potassium ions, on the conformational structure of HDAH Results and Discussion Stabilization of conformational structure of HDAH by inhibitors Taking...
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Báo cáo khóa học: Conformational changes of Newcastle disease virus envelope glycoproteins triggered by gangliosides pdf
Ngày tải lên :
30/03/2014, 13:20
... Blumenthal, R & Herrmann, A (1999) Conformational intermediates and fusion activity of influenza virus hemagglutinin J Virol 73, 4567–4574 17 Garcı´ a- Sastre, A. , Cobaleda, C., Cabezas, J .A & Villar, ... did not allow us to use this assay with intact cells as targets (data not shown) The existence of conformational intermediates for viral proteins such as influenza HA [16], vesicular stomatitis ... Tecnologı´ a, Spain (Grant DGES PM97-0160) We thank Drs E Dı´ ez Espada and J A Rodrı´ guez from Intervet Laboratories (Salamanca, Spain) for providing the lentogenic ÔClone 30Õ strain of NDV Thanks are...
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Báo cáo hóa học: " Research Article A Fixed Point Approach to the Fuzzy Stability of an Additive-Quadratic-Cubic Functional Equation" pdf
Ngày tải lên :
21/06/2014, 20:20
... vruta, A generalization of the Hyers-Ulam-Rassias stability of approximately additive a ¸ mappings,” Journal of Mathematical Analysis and Applications, vol 184, no 3, pp 431–436, 1994 18 J M Rassias, ... Applications, vol 173, no 2, pp 325–338, 1993 67 Th M Rassias and K Shibata, “Variational problem of some quadratic functionals in complex analysis,” Journal of Mathematical Analysis and Applications, ... Rassias, “On the stability of functional equations in Banach spaces,” Journal of Mathematical Analysis and Applications, vol 251, no 1, pp 264–284, 2000 64 Th M Rassias, “On the stability of functional...
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Báo cáo hóa học: " Research Article Strong Convergence to Common Fixed Points of a Finite Family of Nonexpansive Mappings" docx
Ngày tải lên :
22/06/2014, 18:20
... objective of this paper is to show another generalization of Mann and Halpern iterative algorithm to a setting of a finite family of nonexpansive mappings We deal with the iterative scheme x0 ∈ C and ... Analysis and Applications, vol 211, no 1, pp 71–83, 1997 [5] W Takahashi, T Tamura, and M Toyoda, “Approximation of common fixed points of a family of finite nonexpansive mappings in Banach spaces,” ... nonexpanding maps,” Bulletin of the American Mathematical Society, vol 73, pp 957–961, 1967 [9] Y Kimura, W Takahashi, and M Toyoda, “Convergence to common fixed points of a finite family of nonexpansive...
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báo cáo khoa học: "Collaborative planning approach to inform the implementation of a healthcare manager intervention for hispanics with serious mental illness: a study protocol" pptx
Ngày tải lên :
10/08/2014, 11:20
... Scientific Software Development GmBH, Berlin, Germany), a qualitative data management software [58], will be used to manage and analyze all qualitative data Quantitative analysis All tests will ... reduce language barriers and drafting all patient educational materials at the appropriate reading level (e.g., fourth grade) to enhance health literacy Surface adaptations are a prerequisite, ... Scale [77], a 24-item self-report measure that uses separate four-point scales to tap acculturation-related changes in two languages Barriers to medical care: a list of 11 common factors that...
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A mixed methods pilot study with a cluster randomized control trial to evaluate the impact of a leadership intervention on guideline implementation in home care nursing ppsx
Ngày tải lên :
11/08/2014, 05:21
... [http://www.diabetes.ca/cpg2003/chapters.aspx?agrowing healthcareproblem.htm] Canadian Diabetes Association (CDA): Clinical practice guidelines for the management of diabetes in Canada Canadian Medical Association Journal ... with footwear being a major cause of traumatic ulcers [8] Lack of awareness of risk factors associated with diabetic foot ulcer by health care professionals and patients adds to unnecessary morbidity ... related to the clinical application of practice recommendations Senior administrators estimated that approximately 70 percent of staff typically attend 'usual' strategies Experimental Group In addition...
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báo cáo khoa học: " A mixed methods pilot study with a cluster randomized control trial to evaluate the impact of a leadership intervention on guideline implementation in home care nursing" docx
Ngày tải lên :
11/08/2014, 16:21
... [http://www.diabetes.ca/cpg2003/chapters.aspx?agrowing healthcareproblem.htm] Canadian Diabetes Association (CDA): Clinical practice guidelines for the management of diabetes in Canada Canadian Medical Association Journal ... with footwear being a major cause of traumatic ulcers [8] Lack of awareness of risk factors associated with diabetic foot ulcer by health care professionals and patients adds to unnecessary morbidity ... related to the clinical application of practice recommendations Senior administrators estimated that approximately 70 percent of staff typically attend 'usual' strategies Experimental Group In addition...
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Báo cáo khoa học: "The epidemiology of severe sepsis in England, Wales and Northern Ireland, 1996 to 2004: secondary analysis of a high quality clinical" pps
Ngày tải lên :
12/08/2014, 23:22
... frame and were unable to describe changes over time We present data from an analysis of a database arising from a national audit of patient outcomes from critical care units in England, Wales and ... interval Page of 10 (page number not for citation purposes) Case Mix Programme Database The Case Mix Programme Database is a high quality clinical database containing data on demographics, case ... care for England, Wales and Northern Ireland and is co-ordinated by the Intensive Care National Audit & Research Centre (ICNARC) [13] Patient data are abstracted by trained data collectors according...
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