... wild and cultivated sunflower Results SNPs frequency andnucleotide diversity A total of 64 candidate regions related to biotic and abiotic stresses were selected for SNP identification andnucleotide ... Abbreviations SNP singlenucleotide polymorphism, indels short insertions and/ or deletions, SSRs simplesequence repeats, bp base pairs, kbp kilo base pairs, LD linkage disequilibrium, EST expressed sequence ... not be sequenced directly were cloned into pGEMT-easy (Promega, Madison, USA) and at least two clones were sequenced with forward and reverse primers to discard PCR errors The nucleotide sequences...
... and GL provided study materials or patients HSB, PS, JG, AB, CW and GL participated in collection and assembly of data CP and AS participated in data analysis and interpretation CB, HSB, AB and ... doi:10.1186/1479-5876-9-139 Cite this article as: Bergmann et al.: Toll-like receptor singlenucleotide polymorphisms Asp299Gly and Thr399Ile in head and neck squamous cell carcinomas Journal of Translational Medicine ... Informatics, Biometry and Epidemiology, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany Authors’ contributions CB designed the study and participated in data analysis and interpretation...
... and GL provided study materials or patients HSB, PS, JG, AB, CW and GL participated in collection and assembly of data CP and AS participated in data analysis and interpretation CB, HSB, AB and ... doi:10.1186/1479-5876-9-139 Cite this article as: Bergmann et al.: Toll-like receptor singlenucleotide polymorphisms Asp299Gly and Thr399Ile in head and neck squamous cell carcinomas Journal of Translational Medicine ... Informatics, Biometry and Epidemiology, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany Authors’ contributions CB designed the study and participated in data analysis and interpretation...
... samples and participated in the design and analysis of the study, and MN genotyped the Japanese samples JD, JQ, NJ, YX, CY and JW evaluated the patients and genotyped Chinese samples, and TN helped ... Nanjing University, andsinglenucleotide Page of (page number not for citation purposes) polymorphism (SNP) Research Center of RIKEN, and informed consent was obtained from patients and control individuals ... association between the RHOB and TXNDC3 SNPs and knee OA susceptibility, we conducted two case-control studies in Han Chinese and Japanese populations and a meta-analysis Materials and methods A population...
... Incest and mental handicap J Ment Defic Res 1990, 34:483-490 doi:10.1186/gm163 Cite this article as: Beaudet AL: Ethical issues raised by common copy number variants andsinglenucleotidepolymorphisms ... cost effective, is not evidence based and may lead to stigmatization or undue anxiety [15,16] Assuming lowcost and high-throughput genotyping and good physician and patient education, this form of ... disomy causing disorders such as Prader-Willi and Angelman syndromes and in identifying candidate gene regions for disease in children born of first cousin and similar matings However, the occurrence...
... analysis and statistical analyses; and wrote the manuscript HF, YK, SI, TH, MK, IM, ST, YT, HH and TS recruited the patients and controls and collected clinical information NT designed and coordinated ... panel consisting mainly of Chinese and Korean populations, the association of 27 singlenucleotide polymorphisms (SNPs) in the TLR7-TLR8 region with SLE was examined, and a significant association ... intron 2, rs179019 and rs179010, was newly detected (Figure and Table 1) Significant association of rs179019 and rs179010 was observed under the recessive model for the A and T alleles, respectively...
... direction Both forward and reverse sequencing primers were used to maintain quality control Primer sequences and conditions are available upon request The presence of IL-4RA nucleotidepolymorphisms was ... Aspergillus Der p and/ or Der f 43 52 28 37 Cat 46 28 CR 28 22 Trees 78 57 Grasses 56 54 Weeds 68 48 The results of IL-4RA singlenucleotidepolymorphisms (SNP) are seen in Table The presence and allele ... chain; SNP singlenucleotidepolymorphisms Data presented as percentage (%) of patients and in parentheses, allele frequency P value using Fisher’s exact test Knutsen et al Clinical and Molecular...
... Extraction and Report Language PHP Hypertext Preprocessior RDBMS Relational Database Management System SNP SingleNucleotide Polymorphism SSCP Single- Strand Conformation Polymorphism SSR SimpleSequence ... database and integrated SSR finder tool into the BUILDING SSRs DATABASE of Citrus Website After cleaning, masking repeat, vector and organelle sequences, the EST-SSR sequences and the related EST sequences ... có : Dinucleotide SSR (GT)6 GTGTGTGTGTGT Trinucleotide SSR (CTG)4 CTGCTGCTGCTG Tetranucleotide SSR (ACTC)4 ACTCACTCACTCACTC Trinucleotide SSR xuất dinucleotide SSR khoảng 10 lần, tetranucleotide...
... ngẫu nhiên (Randomly Amplified Polymorphic DNA) RFLP: Đa hình độ d i đoạn cắt giới hạn (Restriction Fragment Lengh Polymorphisms) SSR: Đa hình đoạn lặp lại đơn giản (Simple Sequence Repeats) TAE: ... Fragment Length Polymorphisms (AFLP) - Chỉ thị dựa sở chuỗi có trình tự lặp lại (Repeatitive sequences): + Tiểu vệ tinh (Minisatellite) + Đa hình đoạn lặp lại đơn giản - SimpleSequenceRepeats (SSR) ... giống Xuất phát từ mục tiêu trên, tiến h nh đề t i: "Nghiên cứu sử dụng thị phân tử SSR (Simple Sequence Repeats) chọn giống lúa 1.2 Mục đích yêu cầu đề tài 1.2.1 Mục đích Xác định đợc mối quan...
... ngẫu nhiên (Randomly Amplified Polymorphic DNA) RFLP: Đa hình độ d i đoạn cắt giới hạn (Restriction Fragment Lengh Polymorphisms) SSR: Đa hình đoạn lặp lại đơn giản (Simple Sequence Repeats) TAE: ... Fragment Length Polymorphisms (AFLP) - Chỉ thị dựa sở chuỗi có trình tự lặp lại (Repeatitive sequences): + Tiểu vệ tinh (Minisatellite) + Đa hình đoạn lặp lại đơn giản - SimpleSequenceRepeats (SSR) ... giống Xuất phát từ mục tiêu trên, tiến h nh đề t i: "Nghiên cứu sử dụng thị phân tử SSR (Simple Sequence Repeats) chọn giống lúa 1.2 Mục đích yêu cầu đề tài 1.2.1 Mục đích Xác định đợc mối quan...
... products are denatured to become single- stranded, and separated by gel electrophoresis under nondenaturing conditions A single- stranded fragment with a mutation or singlenucleotide polymorphism (SNP) ... labeled with a fluorophore 2.2 Sequences and Samples For the examples shown in Fig and Fig 2, the following sequences, with priming regions typed in lower case and positions and chemical nature of polymorphic ... human diversity panel cSequence 1, see Sequences and samples NED-labeled fragment and at position 325 (T/G) and 346 (T/C) for the ROX-labeled fragment from exon 26 Oligonucleotides labeled with...
... about 30,000 of P pinaster stands, and financial losses were considerable [6] To overcome this problem and to avoid further damage, from 1986 onwards candidate stands for seed collection in the ... environmental conditions, and they may also influence adjacent native stands by pollen and seed dissemination The establishment of forest plantations throughout the world demands increasing amounts ... were used 2.2 CpSSR and terpene analysis Total DNA was extracted according to the Doyle and Doyle [10] protocol with modifications described by Lerceteau and Szmidt [18] and Plomion et al [25]...
... performed by allele-specific PCR and verified by direct sequencing PCR primer and probe sequences for the V14M SNP were 5' -AGTGGAGTGGCTACAAAGGTCCC-3' (forward primer) and 5' CCCATCTCAGCCCTGCTCAC/T-3' ... (reverse primer) PCR primer and probe sequences for the L467P SNP were 5' CATGTGCTAGTGGATCTACT/C-3' (forward primer) and 5' AGTGGAGTGGCTACAAAGGTCCC-3' (reverse primer) Results and discussion In marked ... years (17–81 years), and 76% were female A cohort of 171 healthy individuals matched on the basis of age, sex and origin were used as a healthy control group All protocols and recruitment sites...
... JTF, GJA, PDR, and PK analyzed the data and wrote the manuscript PK and PDR designed the study APC, PDR, and JR sequenced the cultivars, generated the methylation-filtration libraries and performed ... pets, and livestock and are the source of the poison ricin [14] Castor bean plants commonly escape cultivation and are found in disturbed sites such as roadsides, stream banks, abandoned lots, and ... rates using singlenucleotidepolymorphisms Genetics 2000, 154:931-942 37 Wakeley J, Nielsen R, Liu-Cordero SN, Ardlie K: The discovery of singlenucleotide polymorphismsand inferences about human...
... Fallin D, Lanchbury JS: Singlenucleotidepolymorphismsand the future of genetic epidemiology Clin Genet 2000, 58:250–264 33 Gray IC, Campbell DA, Spurr NK: Singlenucleotidepolymorphisms as tools ... Lipshutz R, Chee M, Lander ES: Large-scale identification, mapping, and genotyping of single- nucleotidepolymorphisms in the human genome Science 1998, 280:1077–1082 30 Kruglyak L, Lander E: High-resolution ... constructed This review summarizes current and potential future contributions of one type of DNA sequence variant, singlenucleotidepolymorphisms (SNPs), to our understanding of asthma pathophysiology...
... Thơ, tháng 10 năm 2012 SNP (SINGLE NUCLEOTIDE POLYMORPHISMS) I GIỚI THIỆU VỀ SNP - SNPs (đọc snip) viết tắt từ chữ SingleNucleotide Polymorphisms, có nghĩa dạng đa hình nucleotide đơn Marker thường ... for Gold in Genome Gulch 2002 The Scientist 13 SingleNucleotide Polymorphisms: From the Evolutionary Past 2001 Nature 14 SingleNucleotide Polymorphisms: To a Future of Genetic Medicine ... biến điểm nucleotide genome (hình 1) Hình 1: Một đột biến điểm xảy dẫn đến thay cặp nucleotide G – C A – T ngược lại tạo SNP - Đa hình đơn nucleotide, SNPs biến thể trình tự DNA xảy đơn nucleotide...
... are unavailable (Bindler et al., 2007) 2.9 SimpleSequenceRepeats (SSR) markers Simplesequencerepeats (SSR) are regions of DNA that consist of short, tandem repeated units (2-6 bp in length) ... Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD) and microsatellites or SimpleSequenceRepeats (SSR), have been widely used to estimate genetic variability and phylogenetic studies ... (RFLP), Random Amplified Polymorphic DNA (RAPD) andSimpleSequenceRepeats (SSR) (Bredemeijer et al.,1998; Villand et al., 1998; Park et al., 2004 and Garcia-Martinez et al., 2006) Comparatively,...