single domain antibody library construction
Báo cáo khoa học: Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen doc
... reported the isolation of singledomain antibodies (sdAbs) consisting of the variable domain of these heavy chain antibodies, also named VHH [2] These minimal antibody domains are endowed with ... a single loopdomain in NCA, compared with three tandemly repeated loop-domains in CEA Comparisons between the extracellular domains of CEA and NCA show that the N-terminal and adjacent loop-domains ... importantly, the single- domain nature of VHH permits the amplification and subsequent straightforward cloning of the corresponding genes, without requiring an artificial linker peptide (as for single- chain...
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Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf
... Skelton, N.J (2000) Characterization of the binding interface between the E -domain of Staphylococcal protein A and an antibody Fv-fragment Biochemistry 39, 26–36 Bertini, I., Luchinat, C & Piccioli, ... as a function of the relaxation delay and the data were then fitted by least-squares fitting to a single exponential Correlation times were calculated from the T1/T2 ratios according to the so-called ... native E coli IscU was first estimated by gel filtration The elution profile of E coli IscU presents a single peak at a molecular mass corresponding to that of the monomer (13.9 kDa) Ó FEBS 2004 Characterization...
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Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... nonimmune library construction The scFv library was generated by RT-PCR from total RNA purified from B lymphocytes of human peripheral blood To avoid, as far as possible, a bias in antibody variable ... scFv nonimmune library of 1.1 · 108 variants Evaluation of the library in terms of variability revealed that it contained different combinations of variable domains From this library two anti-Cn2 ... non-immunized phage display library Nat Biotechnol 14, 309–314 22 Sheets MD, Amersdorfer P, Finnern R et al (1998) Efficient construction of a large nonimmune phage antibody library: the production...
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Báo cáo sinh học: " Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide" pptx
... COOH VH VL (Hinge) Results Construction and expression of single chain antibodies against gD Four from the panel of anti-HSV gD hybridomas available were selected for scFv construction based on the ... of the anti-gD single chain antibodies The results were consistent with reconstitution of the original Bacterial expression and extraction of anti-gD single chain antibodies The single chain antibodies ... type GH by administration of a DL11-based single chain antibody before infection with virus The HSV type-common and startling in vitro activities of single chain antibodies derived from hybridoma...
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báo cáo hóa học:" Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide" pdf
... COOH VH VL (Hinge) Results Construction and expression of single chain antibodies against gD Four from the panel of anti-HSV gD hybridomas available were selected for scFv construction based on the ... of the anti-gD single chain antibodies The results were consistent with reconstitution of the original Bacterial expression and extraction of anti-gD single chain antibodies The single chain antibodies ... type GH by administration of a DL11-based single chain antibody before infection with virus The HSV type-common and startling in vitro activities of single chain antibodies derived from hybridoma...
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Báo cáo khoa học: " Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule" pps
... CH2-CH3 region of the human IgG1 antibody; HLA-A2 transmembrane domain: the transmembrane domain of the HLA-A2 protein; VSVG transmembrane domain: the transmembrane domain of the VSVG protein; E3: ... promoter; aCD20 V: the variable domain of the kappa chain of the mouse anti-CD20 antibody; aCD20 Vg: the variable domain of the gamma chain of the mouse anti-CD20 antibody; CH2-CH3 region: the ... virus glycoprotein, membrane-bound single chain antibody against the CD20 antigen with either a HLA-A2 transmembrane domain (SC2H7-A2) or a VSVG transmembrane domain (SC2H7-GS) CMV enhancer: the...
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Investigation of SPR and electrochemical detection of antigen with polypyrrole functionalized by biotinylated single chain antibody a review
... use The antibody is a recombinant single- chain fragment (Sc-Fv Ab) consisting of heavy-chain and light-chain domains covalently linked through a 16 amino-acid peptide The monoclonal antibody ... biotinylated single- chain antibody was specifically achieved, leading to a good dispersion of the Sc-Fv antibody over the polypyrrole surface Fig SPR kinetic curve of different steps of biosensor construction: ... expressed as an antibody Sc-Fv Ab fragment with a His tag biotin residue for binding The antigen is a peptide sequence of 13 aminoacids conjugated to BSA The masses of the single- chain antibody and...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... binding of the PDZ domain to the ephrinB2(301–333)-pY304 peptide has no impact on the binding of the Grb4 SH2 domain Formation of three-component complexes among the SH2 domain, the PDZ domain and ephrinB2(301–333) ... pYMDL for the Abl SH2 domain; pYDHP, pYKFL and pYNR for the CrK SH2 domain; pYDEP, pYDED and pYDEV for the Nck SH2 domain; pYLNV, pYLNV, pYIN and pYMN for the Sem5 SH2 domain; pYMXM, pYVXM, pYIXM ... N-terminal SH2 domain of p85; pYXXM for the C-terminal SH2 domain of p85; pYVIP, pYILI and pYILV for the C-terminal SH2 domain of PLC-r; pYELE, pYIDI and pYVDV for the N-terminal SH2 domain of PLC-r;...
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Tài liệu Báo cáo khoa học: The single tryptophan of the PsbQ protein of photosystem II is at the end of a 4-a-helical bundle domain docx
... different structural domains: a minor N-terminal domain, with a poorly defined secondary structure enriched in proline and glycine amino acids (residues 1–45), and a major C-terminal domain containing ... folds for the region of the sequence corresponding to the N-terminal domain (residues 1–45) As new threading runs excluded this domain, the selection of mainly a-helix templates became even clearer ... nomenclature, which includes two more cases to specify the subunit and the domain (i.e 1xxxA2 ¼ PBD file 1xxx, subunit A, domain 2) The score thresholds for each method with a certainty of >80% are:...
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Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt
... of a single- transmembrane polypeptide, each containing an extracellular ANPbinding domain (ECD), a transmembrane domain, and an intracellular domain consisting of an ATP-binding regulatory domain ... results of our single- particle EM studies described in this article support the above reasoning, and have identified the hh dimer as the only form found in solution The single- particle reconstructions ... results of single- particle EM and Trp fluorescence studies for the transmembrane signaling mechanism of the ANP receptor are discussed Results and Discussion EM and single- particle reconstruction...
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Báo cáo khoa học: "Construction of Domain Dictionary for Fundamental Vocabulary" pdf
... Categorize the article as the top domain If the top domain is NODOMAIN, the article is categorized as the second domain under the condition below DOMAIN )| ữ |W (NODOMAIN)| HEALTH > 0.03 where |W ... web pages, Domain Dictionary Construction To identify which domain a JFW is associated with, we use manually-prepared keywords for each domain rather than document collections The construction ... as one of the domains by the method described in Đ4.1 (iv) Sort the domains by the number of documents in descending order (v) Associate uw with the top domain 5.2 4.2 Domain DIET Domain Estimation...
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Báo cáo hóa học: " Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library" pptx
... use in antibody screening and antibody binding analysis (Table 1) Full-length HAs were constructed by replacing the transmembrane domain present in the HA2 subunit with the trimerization domain ... http://www.virologyj.com/content/5/1/130 Phage display Fab library screening Library screening was performed using the naïve human Fab phage display library HX01 (Humanyx Pte Ltd, Singapore) Antigen targets ... conjugated) was used as the secondary signal producing antibody 1-Untreated IN597-FO; 2-Trypsin-cleaved IN597-FO The humanized protective antibody VN04-2 was used as a positive control for presence...
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Báo cáo hóa học: " Research Article Novel Techniques of Single-Carrier Frequency-Domain Equalization for Optical Wireless Communications" doc
... Journal on Advances in Signal Processing Electrical domain Optical domain Electrical to optical converter (LED) Electrical modulator Electrical domain Optical to electrical converter (photodiode) ... “Frequency -domain equalisation for optical transmission systems,” Electronics Letters, vol 44, no 14, pp 870–872, 2008 C.-C Hsieh and D.-S Shiu, Single carrier modulation with frequency domain equalization ... respectively Throughout the paper, lower cases and upper, are used to represent time domain and frequency domain signals, respectively; and represent linear and circular convolution, respectively;...
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Báo cáo hóa học: " Research Article Frequency-Domain Block Signal Detection with QRM-MLD for Training Sequence-Aided Single-Carrier Transmission" pptx
... “Frequency domain equalization for single- carrier broadband wireless systems,” IEEE Communications Magazine, vol 40, no 4, pp 58–66, 2002 [5] K Takeda, T Itagaki, and F Adachi, “Joint use of frequencydomain ... antenna diversity for single- carrier transmissions,” IEICE Transactions on Communications, vol E87-B, no 7, pp 1946–1953, 2004 [6] K Takeda, K Ishihara, and F Adachi, “Frequency -domain ICI cancellation ... iterative DFE for single carrier modulation,” Electronics Letters, vol 38, no 19, pp 1144–1145, 2002 [8] N Benvenuto and S Tomasin, “Iterative design and detection of a DFE in the frequency domain, ” IEEE...
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Báo cáo hóa học: "Research Article On the Information Rate of Single-Carrier FDMA Using Linear Frequency Domain Equalization and Its Application for 3GPP-LTE Uplink" doc
... the users via a signalling channel in the downlink For simplicity, we start with the single- user single- input single- output system and then extend it to the multi-user case with multiple antennas ... “Frequency domain equalization for single- carrier broadband wireless systems,” IEEE Communications Magazine, vol 40, no 4, pp 58–66, 2002 [2] N Wang and S Blostein, “Comparison of CP-based single ... bandwidth on system spectral efficiency We consider a single user system where all the available subcarriers in the system are occupied by the single user Figure compares the EURASIP Journal on...
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Báo cáo hóa học: " Research Article Frequency-Domain Equalization in Single-Carrier Transmission: Filter Bank Approach" doc
... the reference systems are given FFT BASED FREQUENCY -DOMAIN EQUALIZATION IN A SINGLE- CARRIER TRANSMISSION Throughout this paper, we consider single- carrier block transmission over a linear bandlimited ... the lowercase letters will be used for time -domain notations and the uppercase letters for frequency -domain notations The letter n is used for timedomain 2× symbol-rate data sequences and m for ... equalization include the forward transform from time to frequency domain, channel inversion, and the reverse transform from frequency to time domain The CP is inserted after the symbol mapping in the...
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Báo cáo khoa hoc:" Construction and characterization of a bovine BAC library with four genome-equivalent coverage" pptx
... microsatellite was not found in the library The library was also successfully screened for the 31 marker genes of the Texas standard Construction of a bovine BAC library 547 nomenclature of the bovine ... total library corresponds to four genome-equivalents Therefore the probability of nding any given autosomal locus in the library is 0.98 and given the fact that a male was used to construct the library, ... BAC library was spotted on high density membranes and transferred to 384 well-plates in order to permit screening of the library by hybridisation, in addition to PCR screening The bovine BAC library...
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báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx
... proto-oncogene R2-R3 domain (pdb entry code 1gv2) The figure was drawn with PyMOL [62] (C) Stereo view of the environment of residue R69 within the R2 domain high sequence homology of the MYB domains (Figure ... DNA-Binding Domains and Activation/Repression domains are thought to be spatially independent The yeast twohybrid technique is based on this concept [46] Based on our results (Figure 2), these two domains ... regulation domain can interact with the R2R3 N-terminal domain to alter its intrinsic properties [47] Likewise, in C1, a MYB transcription factor promoting anthocyanin accumulation in maize, the R2R3 domain...
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Báo cáo y học: "Transcription phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des-transmembrane domain" potx
... glycosylation site In this article, the construction of cloning and expression plasmids, expression of UL45Δ fusion protein, production of polyclonal antibody, time course of transcription and ... phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des-transmembrane domain Virology Journal 2010 7:232 Submit your next manuscript to BioMed ... injections the rabbit anti-UL45Δ serum was collected Agar diffusion reaction showed the titer of antibody was 1:32 With the methods of ammonium sulfate precipitation and DEAESepharose column, we...
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Báo cáo y học: " Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients" doc
... BJ, Jiang S: Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E coli cells elicits potent neutralizing antibody and protective immunity Virology ... http://www.virologyj.com/content/7/1/299 Page of 29 He Y, Li J, Jiang S: A single amino acid substitution (R441A) in the receptor-binding domain of SARS coronavirus spike protein disrupts the antigenic ... doi:10.1186/1743-422X-7-299 Cite this article as: Cao et al.: Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients Virology Journal...
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