serum nacl and penicillin
Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"
... range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+, K+ of case and control groups, with a significant difference in serum + Na of case and control ones ... senile cataract (79 female, 76 male) and the control one consisted of 155 of cases’ close relatives (79 female, 76 male) Mean age and standard deviation of patients and control group were 61.96±14.5, ... control group and sent to the laboratory Serum Na+, K+ levels were measured with flam photometry method by the aid of SEAC model FLP20, Italy Outcoming data was assessed by spss 11 software and mean...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... activities of the TRH-DEs in serum, liver and brain from rat, pig and cow Serum and tissue homogenates were prepared and analyzed as described in Materials and methods (n ¼ for pig and cow, n ¼ for rats, ... porcine serum Preparation of tissue homogenates and serum After the animals were killed, blood and tissues were immediately collected Serum was obtained after clotting overnight at °C and centrifugation ... L in human and rat), 607 (T in pig and human; M in rat), 614 (I in pig and human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human), 991 (A in pig and human; S...
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Báo cáo y học: "Serum, urinary, and salivary nitric oxide in rheumatoid arthritis: complexities of interpreting nitric oxide measures" doc
... urine and saliva, elevated serum NOx and serum NOx/creatinine, normal renal creatinine clearance, and reduced renal NOx clearance and fractional excretion The reduced renal NOx clearance and fractional ... urine and serum Urine and serum samples serum from 25 patients with active rheumatoid arthritis (RA) and 20 healthy controls (N) were analyzed 72 hours after initiation of a diet low in nitrate and ... protein, serum TNF, and serum IL-6 [14] These workers did not control NOx intake [14,17] Onur and colleagues showed that patients with RA had higher serum NOx levels than controls, and that NOx...
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Báo cáo y học: "Serum proteins and paraproteins in women with silicone implants and connective tissue disease: a case–control study" pot
... with dermatomyositis had two IgG(κ) bands and one IgG(λ) band, and a woman with polymyositis had both IgG(κ) and IgM(λ) bands The total number of paraprotein bands was consequently the same in the ... TPO, and EAS designed the study GC, FWM, RC, EAS, AT, TPO, DJC, and HJW acquired the data GC, FWM, RC, and AT analyzed and interpreted the data GC and FWM prepared the manuscript GC, FWM, and ... no silicone implants); CTD+ and S+, Group (74 women with CTD and with silicone implants); CTD- and S-, Group (14 women with no disease and no silicone implants); CTD- and S+, Group (14 women with...
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Báo cáo y học: "The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease" potx
... Materials and methods Intervertebral disc cell culture IVD cells were isolated from the NP of adult bovine caudal IVDs by enzymatic digestion and expanded in monolayer culture in standard Dulbecco's ... cells per millilitre and a final alginate concentration of 1.2% The alginate beads were then incubated in combinations of medium supplemented with or without serum and glucose and in 21% or 1% oxygen, ... atmospheric levels of oxygen and in medium supplemented with 20% FCS and 320 mg/dL glucose Cell proliferation and cell viability Monolayers were harvested by trypsinisation and viable cell counts were...
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Báo cáo y học: "Central fatigue and nycthemeral change of serum tryptophan and serotonin in the athletic horse" potx
... hydroxylase (TPH) and aromatic L-amino acid decarboxylase [2,11] 5-HT is a metabolic precursor of melatonin in the pineal gland and is believed to be involved in the control of sleep and in clock ... 18:49) and natural indoor temperature (19–21°C) All the horses were feed traditional rations, based hay and a mix of cereals (oats and barley), it was provided three times daily (at 08:00, 12:00 and ... with a standardized procedure and stored at + 4°C for a maximum of 24 h Individual serum samples were deproteinized with 5-sulfosalicylic acid (5-SSA), centrifuged for 10 at 3000 rpm, and immediately...
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Báo cáo y học: "Parenteral versus enteral nutrition: effect on serum cytokines and the hepatic expression of mRNA of suppressor of cytokine signaling proteins, insulin-like growth factor-1 and the growth hormone receptor in rodent sepsis" pps
... Authors' contributions MJO'L and RCS conceived of and designed the study All authors participated in the animal handling and procedures CJS carried out the immunoassays and AX performed the PCR studies ... Sham group) and received the same feeding regimen as the CLP animals (Sham-PN group, n = 8; Sham-EN group, n = 6; and Sham-Starve group, n = 12) The PN and EN solutions were identical and provided ... intraperitoneal fluid and analgesia were administered 24 and 48 hours following operation Cecal ligation and puncture procedure Following placement of the stomach catheter, in animals randomized to CLP...
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Báo cáo y học: " Effect of cardiopulmonary bypass on activated partial thromboplastin time waveform analysis, serum procalcitonin and C-reactive protein concentrations" docx
... clinical and biological tests fail to detect postoperative infection in the cardiac surgery setting This can delay the diagnosis and treatment of sepsis and may increase postoperative mortality and ... (CRP) and procalcitonin (PCT) have been studied after CPB [5,6] Serum CRP values increase during the postoperative period after cardiac surgery even in the absence of infection [7] and even if serum ... systemic inflammatory response syndrome time [10] and not influenced by anticoagulants or coagulation factor deficiencies Downey and colleagues [10] and Toh and colleagues [12] have shown that the BPW...
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Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"
... presence and persistence of IgG and IgE anti-influenza antibodies in the serum from those with normal and ele- 243 vated levels of IgE are understandable Our findings appear to be of importance and ... RESULTS Characteristics of Study Subjects Serum IgE levels and IgE and IgG anti-Influenza virus antibodies were studied in children (N=3) (m/f 14-16 y/o) and adults (N=3) (m/f, 41-49 y/o) approaching ... Total serum IgE levels were both normal and elevated in adults and children vaccinated with Influenza virus Children with no history of either Influenza virus infection or vaccination had serum...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... Sample pretreatments and proteomic analysis in the proteomic profiling analysis, the serum samples from the diseased and control groups were randomized, and blinded to investigators Serum samples were ... of Serum Samples in Patients with CRC and Healthy Controls The protein profile of the serum samples from the 98 patients with CRC and the 80 healthy controls were extracted by magnetic beads and ... and had the informed consent of the patients and volunteers The patients and serum samples were then divided into two groups: the ‘‘training’’ set and the blinded ‘‘test’’ set (Table 2).The blood...
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Tài liệu Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays doc
... albumin, serum blood urea nitrogen (BUN), and serum creatinine levels (Table 1) Comparison of the serum concentrations of 22 cytokines, chemokines, and growth factors demonstrated that sCD40L and ... increases in the serum concentrations of sCD40L and TGF-a and significant decreases in the serum concentrations of G-CSF, GM-CSF, and MCP-1 Future studies will focus on understanding the significance ... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... presence and absence of 0.1 lM angiotensin II and IL-10 BrdU incorporation into cardiac fibroblast under normal 10% fetal bovine serum or serum- free culture conditions IL-10 also decreased type I and ... fetal bovine serum and 100 UÆmL)1 penicillin ⁄ streptomycin and incubated at 37 °C in a humidified tissue culture incubator containing 5% CO2 The medium was replaced h after plating and 24 h later ... above and exposed to hypoxia ⁄ SD for h The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄...
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Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc
... ng standard Gb3 and was able to detect > ng Gb3 per lL serum sample Gb3 in the serum of wildtype mice was below the background of this assay MDR1 inhibition and GSL storage disease ERT and CsA ... with 1· NaCl ⁄ Pi solution, sections were blocked with 1% normal goat serum ⁄ NaCl ⁄ Pi (NGS NaCl ⁄ Pi) for 20 Without washing, sections were then stained with VT1 (200 ngÆmL)1 in NGS NaCl ⁄ Pi) ... 0.028) The recovery of serum Gb3 post ERT was found to be, to some extent, variable and some mice within both the control and treated groups did not recover detectable serum Gb3 by the 9-week...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... of membrane lipids, hormones and second messengers, and serve as an important source of metabolic energy Although the binding of fatty acids to human and bovine serum albumin has been thoroughly ... sites, and at least three further low affinity sites have been envisaged NMR studies on tryptic and peptic fragments of bovine serum albumin have localized two high affinity sites in domain III and...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... Tables and thus present only the novel data: protonation constants for DAHKam and VIHN, and stability constants (log a values) of Cu(II)-VIHN, Cu-DAHKam and Ni-DAHKam systems The parameters of CD and ... Ni(II) and Cu(II) complexes of peptides at I = 0.1 M (KNO3) and 25 C Standard deviations on the last digits are given in parentheses EPR The X-band EPR spectra of Cu(II) complexes of VIHN and DAHKam ... considered Comparison of Cu(II) and Ni(II) binding between model peptides and albumins Afnity for Ni(II) at site I can be compared between albumins on one hand and DAHam and DAHKam on the other Much...
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Tài liệu Báo cáo Y học: Transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors potx
... structure and regulation could be conserved but SRF expression patterns and SRF-dependent promoters could have changed during evolution Further understanding of the evolution of SRF and SRFdependent ... supplemented with 10% fetal bovine serum and mM glutamine Embryonic fibroblastoid cells NIH3T3 were cultured in DMEM supplemented with 10% neonatal bovine serum and mM glutamine Cells were transfected ... promoters, c-fos and Act403, and had no significant effects on the weaker CA and Act403mut promoters The activity of the SRFs from these three species was also tested for c-fos and CA promoters...
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Identification of serum proteomic biomarkers for early porcine reproductive and respiratory syndrome (PRRS) infection pptx
... = pH4, F5 = pH3, and F6 = organic solvent The fractions F1, F4, and F6 were analyzed on the three surfaces CM10, IMAC30, and H50 at both low and high focus masses Fractions F2 and F3 were excluded ... LMW and low abundance region of the serum proteome, and specific protein fragments of the serum peptidome have been shown to contain a rich source of disease-specific diagnostic information and ... fractions F1, F4, and F6 on the surfaces CM10, IMAC30, and H50 showed generally good signal intensities and low coefficient of variation (CV) values (< 30%) in both the discovery and validation...
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
... high-afnity and low-afnity sites of tHSAheme-Fe(III) and HSAheme-Fe(III) [represented by a and (1 a), respectively, in Eqn (1)] are 0.18 and 0.82, and 0.14 and 0.86, respectively (Table 1) Effect of ibuprofen ... (1971) Hemoglobin and Myoglobin in their Reactions with Ligands North Holland Publishing Co., Amsterdam and London Goldstein S, Lind J & Merenyi G (2005) Chemistry of peroxynitrites and peroxynitrates ... nal ibuprofen concentration ranged between 1.0 ã 10)6 and 1.0 ã 10)2 m Peroxynitrite was synthesized from KO2 and NO or from HNO2 and H2O2, and stored in small aliquots at )80.0 C [29,30] The peroxynitrite...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... of penicillin G binds with the thiazolidine ring to the enzyme via hydrophobic interactions between the 2b-methyl group and aF146 and bF71 and hydrogen bonding between its carboxylate group and ... of the enzyme and mutants [4,9–13], investigations into the individual rate and binding constants underlying the kinetic properties and biocatalytic performance in the synthesis and hydrolysis ... purchased from Syncom (Groningen, the Netherlands) PGPNA, ampicillin, penicillin G, 6-APA and 7-ADCA were obtained from DSM-Gist (Delft, the Netherlands) PAPNA was prepared as follows: phenylacetylchloride...
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Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf
... and incubated for 15 at 30 °C It was then centrifuged again in a Microcon tube (second ultrafiltrate) The prohepcidin levels of the original serum, the first serum ultrafiltrate and the second serum ... acid and then incubated with 20 lL of anti-A1AT IgG After incubation, the tip was washed three times with 10 lL of NaCl ⁄ Pi (pH 7.4) and blocked with 20 lL of 20 mgÆmL)1 bovine serum albumin, and ... again with NaCl ⁄ Pi Human serum (15 lL) concentrated with Microcon YM-30 was pipetted up and down for The proteins which did not bind to the column were eliminated with two NaCl ⁄ Pi and Milli-Q...
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