separation and cryopreservation of lymphocytes from spleen and lymph node
Báo cáo khoa học: "Independent position correction on tumor and lymph nodes; consequences for bladder cancer irradiation with two combined IMRT plan" pptx
Ngày tải lên :
09/08/2014, 09:20
... pelvic lymph nodes, femoral heads and small bowel were delineated as well In consideration of daily on-line position correction, a CTV - PTVtumor margin of mm and a lymph node (ln) PTVln margin of ... elective dose of 40 Gy was prescribed to the lymph nodes and healthy part of the bladder The patients were treated with a full bladder They were instructed to void the bladder and drink 250 cc of water ... example of the dose distribution of a boost plan, an elective plan and the composite dose distribution The requirement of the plans was that 99% of the volume of the target received 95% of the...
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Báo cáo hóa học: " Algorithms for Blind Components Separation and Extraction from the Time-Frequency Distribution of Their Mixture" docx
Ngày tải lên :
23/06/2014, 01:20
... resolution in both time and frequency; however, it suffers from the presence of crossterms for a multicomponent signal These cross-terms result from the interaction of different components of the signal ... components -separation algorithm is illustrated in Figure 3, and Algorithms and Figure provides the algorithm flowchart, Algorithm summarizes the estimation technique of the number of components, and ... take a slice of the TFD T(t, f ) (2) Search and count the number of peaks in each slice (3) Evaluate the histogram of the obtained set of peaks numbers (4) Estimate the total number of the signal...
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Báo cáo y học: "Flow cytometric assessment of the signaling status of human B lymphocytes from normal and autoimmune individuals" doc
Ngày tải lên :
09/08/2014, 01:23
... synthesis of IκB proteins Following engagement of CD40 on Ramos B cells with rCD154, the phosphorylation of IκBα and the degradation of IκBα, -β and -ε were detected by Western Blotting (Fig 2c) and ... (FSC) Assessment of NF-κB activation in human B lymphocytes by multiparameter intracellular flow cytometric analysis of IκB isoforms (-α, -β, -ε) and the phosphorylation status of IκBα R2G6 cells ... (a) and (b) Insets of histograms of fluorescence intensity of the channel that detects the fluorochrome conjugated secondary antibody are shown in (a) and (b) The percentage of positive cells and...
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Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx
Ngày tải lên :
12/08/2014, 23:23
... translation of Nef and Tat was shown to increase the activation state of resting Tlymphocytes, thereby rendering them more amenable to productive infection [13] The expression of early gene products from ... downregulation of each of cell surface CD4 and the CXCR4 [34,35] and CCR5 [36] coreceptors has the benefit of restricting superinfection This may protect the virus within the cell from cellular ... advantage of the capacity of unintegrated DNA to express Tat and Rev and thereby induce GFP expression [20] Introduction of the D116N mutation into the integrase domain renders integrase inactive, and...
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ISOTOPE HYDROGRAPH SEPARATION FOR MODELING OF RUNOFF MECHANISMS OF ATMOSPHERICALLY DERIVED CHEMICAL AND RADIOACTIVE POLLUTANTS
Ngày tải lên :
05/09/2013, 09:08
... composition of 2H and 18O The compositions of the isotopes 2H (D) and 18O were analyzed to obtain δD (‰) and δ18O (‰), respectively, which are defined as the relative deviation of the parts per thousand ... elements such as Ca and Mg This indicates reasonability of this method of load separation in this case It was found that Al, Ti, Y, U, and Th have similarly Journal of Water and Environment Technology, ... 22-23 of 2003 The amount of precipitation was 30 mm in total Figure shows the precipitation record of the studied event (Fig 2a) and the record of the river flow rate (solid line, Fig 2b) of the...
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Removal of arsenic from synthetic groundwater by adsorption using the combination of laterite and ironmodified activated carbon
Ngày tải lên :
05/09/2013, 09:38
... The BET (Brunauer, Emmett and Teller) surface area is 70.19m²/g, pore volume of 0.00062cm3/g and pore size of 126Å Preparation of AC-Fe AC from coconut shell was taken from MAPECOH, Inc (Philippines) ... LA/AC-Fe ratio of 0.3m/0.2m, flow rate of to 6mL/min (or space velocity of 0.98-1.47m3/m3h), pH of 7-8, and initial arsenic concentration of 0.1-0.5mg/L, to obtain a breakthrough time of more than ... (25oC) and atmospheric condition The samples were taken then acidified and analyzed for residual arsenic concentration A mixture of 70% As[III] and 30% As[V] was used in all of the experiments and...
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Characteristics of Leachate from Citrus Groves and their Changes in the Collecting Reservoirs in Matsuyama, Japan
Ngày tải lên :
05/09/2013, 10:17
... western side of the region faces the Seto Inland Sea, around which are Fig.1 - Location of study area and distribution of citrus groves in Matsuyama region - 112 - Journal of Water and Environment ... were collected from points Four samples of leachate were collected from the upper part of the grove The collection points Le.1, Le.2, Le.3, and Le.4 have elevations of 76m, 69m, 66m, and 60m, respectively ... concentrations of dissolved Al and Mn became lower The pH value further increased and it reached basic conditions in the reservoir, and dissolved Al and Mn were removed more from the water On the other hand,...
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Tài liệu The King''''s Post Being a volume of historical facts relating to the Posts, Mail Coaches, Coach Roads, and Railway Mail Services of and connected with the Ancient City of Bristol from 1580 to the present time pdf
Ngày tải lên :
17/02/2014, 02:20
... the morning from London, Bristol, Birmingham, and all parts of the North and West, and also from Scotland and from all parts of the north of Ireland Should this object be attained, the intended ... and their districts—viz.: Hounslow—between and 10 at night from London; between and in the morning from Bristol Maidenhead—between 11 and 12 at night from London; between and in the morning from ... shopkeepers in the city of Bristol, Common Council of the city of Bristol, Mayor, Burgesses and Commonality of the city of Bristol, Mayor, Aldermen and Common Councilmen of the city of Bristol." On March...
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Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx
Ngày tải lên :
19/02/2014, 16:20
... properties of muscle phosphofructokinase Mol Cell Biochem 57, 147–154 Hand SC & Somero GN (1983) Phosphofructokinase of the hibernator Citellus beecheyi, temperature and pH regulation of activity ... Phosphofructokinase: mechanism of the pH-dependent inactivation and reactivation of the rabbit muscle enzyme J Biol Chem 251, 5630–5636 Bock PE & Frieden C (1976) Phosphofructokinase: role of ligands ... ice and were then stored at )80 °C until use All biochemicals and coupling enzymes were obtained from Boehringer Mannheim (Montreal, PQ) or Sigma Chemical Co Purification and standard assay of...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
Ngày tải lên :
20/02/2014, 23:20
... CSD ESI-FT-ICR mass spectrum of de-O-acylated LPS from Fig Negative ion MALDI-TOF mass spectra of lipid A isolated from C trachomatis serotype E (A) and L2 (B) and from Chl psittaci 6BC (C) C ... Isolation of mononuclear cells and granulocytes ¨ from human blood Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at g Scand J ... a ratio of : : 0.01 (v/v/v) and sprayed at a flow rate of lLÆmin)1 MALDI-TOF MS of LPS and free lipid A were performed on a Reflex III (Bruker-Daltonik, Bremen, Germany) in the linear TOF configuration...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
Ngày tải lên :
21/02/2014, 15:20
... of parabutoporin, opistoporin, melittin and mastoparan in absence and presence of mM extracellular Mg2+ ions Bacteria were incubated with twofold serial dilutions of peptides and inhibition of ... venom of spiders and insects Parabutoporin and opistoporin were synthesized chemically and preliminary studies on antibacterial activity showed that the quality and biological activity of native and ... obtained from the blood of healthy volunteers and purified by Ficoll–Paque centrifugation and hyposmotic lysis of red blood cells as described previously [4] Procedure for testing of inhibition of superoxide...
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Prevention and Management of Complications from Gynecologic Surgery pdf
Ngày tải lên :
06/03/2014, 12:20
... clindamycin and gentamicin, clindamycin and ciprofloxacin, clindamycin and aztreonam, metronidazole and gentamicin, or metronidazole and ciprofloxacin.26 In patients with known history of MRSA infection ... discussion of diagnostic and therapeutic treatment options, and of the spectrum of adverse events that may occur with each, appropriately adjusted to fit the risk profile of the patient a Department of ... preventing and managing complications from gynecologic surgery William F Rayburn, MD, MBA Department of Obstetrics and Gynecology University of New Mexico School of Medicine MSC10 5580, University of...
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The Cavalier Songs and Ballads of England from 1642 to 1684 Edited doc
Ngày tải lên :
06/03/2014, 13:20
... never-to-be-superseded topics of love, war, and wine The people of our day have little heart or appreciation for song, except in Scotland and Ireland England and America are too prosaic and too busy, and the masses, ... Satires in the form of a litany were common from 1646 to 1746, and even later From an extempore prayer and a godly ditty, From the churlish government of a city, From the power of a country committee, ... altar, From oaths and covenants, and being pounded in a mortar, From contributions, or free-quarter, Libera, etc From mouldy bread, and musty beer, From a holiday's fast, and a Friday's cheer, From...
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Air Pollution Burden of Illness from Traffic in Toronto: Problems and Solutions potx
Ngày tải lên :
06/03/2014, 16:20
... implementation of sustainable transportation policies and programs in Toronto Fostering and enabling the expansion and use of public transit and active modes of transportation, such as walking and cycling, ... efficiently, offers choice of transport mode, and supports a vibrant economy; and limits emissions and waste, minimizes consumption of non-renewable resources, re-uses and recycles its components, and ... health effects of vehicle pollution; and, a quantitative assessment of the burden of illness and economic costs from traffic pollution in Toronto This report also examines air pollution and traffic...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
Ngày tải lên :
07/03/2014, 05:20
... Comparisons of the thermodynamics and kinetics of the folding of proteins from mesophilic and thermophilic ⁄ hyperthermophilic organisms can provide an insight into the mechanisms of stabilization ... decreases from 79 to 20 kJÆmol)1 when the pH drops from to 2.5 [15] Analysis of a structural model of AmFd suggests that a combination of additional surface ion pairs, the zinc cofactor, and an ... calorimetry and chemical denaturation of Sac7d J Mol Biol 264, 784–805 43 Zeeb M, Lipps G, Lilie H & Balbach J (2004) Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus...
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Best Practices in Social Media / Summary of Findings from the Second Comprehensive Study of Social Media Use by Schools, Colleges and Universities docx
Ngày tải lên :
08/03/2014, 19:20
... measures, such as anecdotal evidence and focus groups, and quantitative measures, such as counts of usage and surveys, used in your evaluation of the success of your social media efforts? (Skip ... sustain and protect our brand 3% 7% 30% 40% 20% 3.7 Demand from alumni or other constituents 6% 15% 31% 35% 13% 3.4 Use of social media by peer institutions 4% 13% 43% 32% 9% 3.3 Strong support from ... media Another unit manages all of our social media activities, without any input from us Another unit manages all of our social media activities, with input from us We handle our own social media...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
Ngày tải lên :
15/03/2014, 11:20
... 2.88 2.72 3.18 2.84 Od2 of Asp423 OH of Ser425 NH of Ala490 NE1 of Trp664 O3 O6 O7 O7 2.89 2.64 2.94 2.40 NH of Ala490 Od2 of Asp636 Od1 of Asp524a Od2 of Asp524a O7 2.63 OH of Tyr590 NAG3 ()1) NAG4 ... E526A and D524A mutants and refined them to ˚ resolutions of 1.80 and 1.76 A, respectively A summary of crystallographic data collection and refinement statistics is given in Table Superimposition of ... superimposition of ligand (substrate or inhibitor)-free and ligand-bound structures In (F), only the ligand-free structure is shown because no ligand-bound structure is available The side-chains of three...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
Ngày tải lên :
16/03/2014, 13:20
... subclass of SR-BI, which mediates binding of LDL and selective uptake of HDL cholesteryl esters and the efflux of cholesterol [30,31] SR-BI has previously been found in a caveolinenriched fraction of ... and analysed for caveolin content by SDS ⁄ PAGE and immunoblotting with anticaveolin-1 antibodies A broad peak with a shoulder of caveolin suggested heterogeneity and the partial separation of ... overlayered with 35%, 26% and 5% sucrose Equal volumes of fractions were collected from the bottom of the tube, membranes were collected by centrifugation, and analysed by SDS ⁄ PAGE and immunoblotting...
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Báo cáo khoa học: Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna ppt
Ngày tải lên :
16/03/2014, 13:20
... instead of reflecting actual mass differences [20], the protocol of Weber and Osborn [21] was employed: in this case, only one band for both Hb from the hemolymph of pale D magna and Hb from red ... the separation of D magna Hb subunits into three different bands (Fig 4A) with molecular masses of 40.0, 38.1 and 35.1 kDa (pale D magna) and 40.0, 36.9 and 35.1 kDa (red D magna) As such separations ... system used for gel electrophoresis on the band pattern of pale and red Daphnia magna hemoglobin (Hb) The hemolymph of pale (2.0 lg of Hb) and red (2.0 lg of Hb) D magna was subjected to electrophoresis...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
Ngày tải lên :
16/03/2014, 14:20
... of analytical grade E D C B A Isolation of CS from cartilage and DS from lung CS was isolated from fresh articular and tracheal cartilages as previously described [4,5], and DS was isolated from ... availability of comprehensive parameters describing the structures of a wide range of oligosaccharide structures The complete assignments of 1H and 13C NMR spectra from a series of disaccharides and tetrasaccharides ... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of...
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