sensitivity to gaba agonists
Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... had been resolved To ensure that the flotation was due to binding to light vesicles, 1% Triton X-100 and 1% SDS were added to dissolve the membranes, as demonstrated for BIP, synaptophysin and biotinylated ... DAT-mediated DA toxicity HEK-293-MSR cells transfected with DAT + empty vector, p25a + empty vector, a-syn + empty vector, DAT ⁄ P25a and DAT ⁄ a-syn to obtain equal concentrations of vector DNA were ... dissolve the vesicles prior to analysis This caused the biotinylated p25a tracer to remain in the bottom fractions, thus demonstrating that the flotation was due to binding to vesicles Because the...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... 10 15 GTP-RhoA Total-RhoA GTP-Cdc42 Total-Cdc42 GTP-Cdc42 Total-Cdc42 GTP-Rac1 Total-Rac1 10 15 GTP-RhoA Total-RhoA GTP-Rac1 CTGF/CCN2 gene regulation via Rho GTPase signaling Total-Rac1 Serum ... actin cytoskeleton To determine whether actin cytoskeleton organization is critical for CTGF/CCN2 gene expression, we examined the effects of latrunculin B on CTGF/CCN2 mRNA levels in response to ... of evidence support an obligatory role for the actin cytoskeleton in the regulation of specific genes by small GTPase proteins The morphology of the actin cytoskeleton upon treatment of the cells...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
... M Borralho et al miR-143 modulates 5-FU cytotoxicity A B Fig mir-143 overexpressing cells are more sensitive to 5-FU cytotoxicity Cells were exposed to either 1–10 lM 5-FU or no addition (control) ... overcome tumour cell resistance to chemotherapy and to increase drug efficacy, thereby minimizing toxic effects, are critically important The molecular mechanisms of 5-FU cytotoxicity have been characterized, ... shown to induce apoptosis in colon cancer cell lines [20,21] The ability of tumour cells to evade apoptosis is an enormous obstacle for effective treatment Consequently, strategies aiming to overcome...
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Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx
... to allow the cells to survive However, it is possible to discern the type of response of each gene by modifying their expression and testing how this modification affects MTX cytotoxicity As tools ... enhancing apoptosis through blocking the advancement of replication forks We took advantage of the down-regulation of TOP1 in MTX-resistance as a way to increase the sensitivity to MTX Indeed, ... and camptothecin, a higher degree of cytotoxicity than with camptothecin alone was achieved in HT29-R This illustrates a strategy for cancer therapy based on using as a modulator an inhibitor of...
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Báo cáo khoa học: Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast docx
... render cells much more sensitive to Hsp90 inhibitor drugs To test whether mutations in the native Hsp90 chaperone of yeast influence sensitivity to Hsp90 inhibitors, we tested eight S cerevisiae ... vivo sensitivity to Hsp90 drugs (Fig 1A), despite the fact that GA and RD are both potent inhibitors of this ATPase [7,8] Of the two Hsp82 mutations associated with high in vivo sensitivity to ... Dpdr5 mutant to be hypersensitive to both drugs and the Dsnq2 mutant to be slightly sensitive to RD (Fig 4B) Pdr5p is a broad-specificity ABC transporter that provides resistance to a wide range...
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What determines banks’ sensitivity to money market interest rates? pot
... of b k u b an s 50 40 30 20 37 10 19 18 11 3 0,5 to 1,0 1,0 to 1,5 1,5 to 2,0 >=2,0
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... J Relat et al decreased sensitivity to malonyl-CoA inhibition (higher IC50), whereas human CPT1B [7,8] exhibits a high Km for carnitine and increased sensitivity to inhibition by malonyl-CoA ... compared to that of human CPT1B] [23] D17E as a malonyl-CoA sensitivity determinant of CPT1B Pig CPT1A has been successfully used to perform chimera studies with rat CPT1A [16] Therefore, to highlight ... the pig N-terminal fragment is able to change the malonyl-CoA sensitivity of the human enzyme, we propose that the pig enzyme can be used as a tool with which to investigate the molecular differences...
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Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf
... mammalian systems as well, alterations to the Hsp90a ⁄ Hsp90b ratio (as with heat shock) may be a significant factor affecting sensitivity of cells to Hsp90 inhibitors Up to now, the Hsp90a ⁄ Hsp90b isoform ... Hsp90b markedly affects cellular sensitivity to the Hsp90 inhibitor radicicol We recently reported that strain PP30[hHsp90b] is extremely sensitive to Hsp90 inhibitors [35] This, however, is not ... in yeast Drug sensitivity assays Cells were inoculated into liquid indicated level of inhibitor, to · 105 cellsÆmL)1 Final cell density growth at either 30 °C or 37 °C, legend to Fig DO containing...
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Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... Ca2+-binding proteins is unique to CaN [4] Similar to Ca2+/CaM-dependent kinases, Ca2+/CaM-binding to CnA Ó FEBS 2002 activates the enzyme by relieving inhibition due to an autoinhibitory domain, which is ... potent inhibitor of both CaNa and CaNb than CyPA/CsA Activation of CaNa and CaNb phosphatase activity by FKBP12/FK506 toward pNPP In contrast to the inhibition of CaN phosphatase activity toward phosphopeptide ... CaN heterodimers towards two peptide substrates, and pNPP We also compared the inhibition of CaN phosphatase activity toward the two peptide substrates by the CaN autoinhibitory peptide, and...
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Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III docx
... expression increases sensitivity to H2O2-mediated apoptosis in complete contrast to the protective effect of other apoptosisinducing agents Increased sensitivity is primarily due to the transcriptional ... Rat1 cells to H2O2 treatment and had no effect upon apoptosis induced by treatment with taxol (Fig S2) caIII expression was restored in 5.61 cells to determine if the sensitivity to H2O2 treatment ... expressing Evi1 have increased sensitivity to H2O2-induced apoptosis Although previous studies have shown that Evi1 protects cells from a variety of inducers of apoptosis, the effects of H2O2 have...
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Báo cáo khoa học: Demethylation of radiolabelled dextromethorphan in rat microsomes and intact hepatocytes Kinetics and sensitivity to cytochrome P450 2D inhibitors pot
... therefore be desirable to assess the potential of a drug to cause CYP inhibition in isolated hepatocytes Isolated hepatocytes have been used extensively to study drug metabolism, cytotoxicity, and induction ... CYP in cultured cells relative to microsomal preparations The objective of this study was to compare the inhibitory potencies of CYP inhibitors in microsomes and hepatocytes We developed an assay ... appeared to be a trend towards lower IC50 values in intact hepatocytes This may be due to differences between the concentrations of free drug available for enzyme inhibition in the two systems To test...
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Báo cáo hóa học: " Luminescence of colloidal CdSe/ZnS nanoparticles: high sensitivity to solvent phase transitions" ppt
... pulse duration The photoluminescence from NPs is collected by a home-built confocal microscope and delivered to a 0.75-m-long imaging monochromator coupled with a single-photon sensitive electron-multiplication ... curves to Varshni relation for dry and inliquid NPs, respectively It is clearly seen that PL peak energy of in-liquid NPs exhibits not only the monotonic temperature dependence similar to dry ... [11] observed increase of photoluminescence peak energy with pressure for dilute dispersions of CdSe nanocrystals in toluene or 4-ethyl pyridine and attributed this to the pressure dependences...
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Báo cáo y học: "Reproducibility and sensitivity to change of various methods to measure joint space width in osteoarthritis of the hip: a double reading of three different radiographic views taken with a three-year interval" pot
... 0.54 0.09 ± 0.36 -0.01 to +0.19 -0.63 to +0.81 0.81 (0.69–0.89) -0.61 (-0.65 to -0.57) -0.49 ± 0.67 -0.05 ± 0.16 -0.09 to -0.01 -0.37 to +0.27 0.97 (0.95–0.98) -0.82 (-0.84 to -0.80) -0.47 ± 0.62 ... 0.02 ± 0.18 -0.2 to +0.06 -0.34 to +0.38 0.95 (0.92–0.97) -0.76 (-0.77 to -0.73) -0.39 ± 0.61 -0.46 ± 0.59 0.07 ± 0.30 -0.01 to +0.15 -0.53 to +0.67 0.87 (0.78–0.92) -0.64 (-0.67 to -0.61) -0.49 ... (-0.74 to -0.70) -0.46 ± 0.59 -0.37 ± 0.50 -0.09 ± 0.40 -0.21 to +0.03 -0.89 to +0.71 0.73 (0.57–0.84) -0.77 (-0.80 to -0.74) -0.47 ± 0.67 -0.52 ± 0.64 0.05 ± 0.23 -0.10 to +0.11 -0.41 to +0.51...
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Báo cáo y học: "RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cells" pps
... our study to observe the consequences to cancer cells treated with both rADI and RNA interference to AS expression Because AS has been reported to play a crucial role in resistance to treatment ... to several anticancer effects, including anti-proliferation and apoptotic cell death [26] Besides, mTOR pathways are controlled by numerous upstream regulators, such as AMPK and phosphoinositol-3 ... mammalian translation factors by nutrients Eur J Biochem 2002, 269:5338-5349 26 Baldo P, Cecco S, Giacomin E, Lazzarini R, Ros B, Marastoni S: mTOR pathway and mTOR inhibitors as agents for cancer...
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Báo cáo y học: " Varied sensitivity to therapy of HIV-1 strains in CD4+ lymphocyte sub-populations upon ART initiation" docx
... as opposed to effector memory cells and serum indicates that during early therapy the plasma and effector memory cell compartments are more susceptible to the effects of the drugs To investigate ... effector memory cells in three out of four patients reflects increased sensitivity to therapy of virus within this cell subset It has been speculated that such changes can influence co-receptor ... made resistant to stromal cellderived factor 1alpha contains mutations in the envelope gp120 but does not show a switch in coreceptor use J Virol 1998, 72:4032-4037 27 Ince WL, Harrington PR, Schnell...
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báo cáo khoa học: " Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress" pdf
... the higher sensitivity of mature leaves to oxidative stress relative to young, developing leaves [e.g [31,44]] This observation indicates that the increased sensitivity of pdx1 to photooxidative ... membranes and to migrate to the cytoplasm [56] Therefore, since the light stress conditions used in this work to induce photooxidative damage were rather drastic (1500 μmol photons m-2 s-1 at ... ultra-weak photon emission with a moveable photomultiplier and CCD imaging J Photochem Photobiol B:Biol 2006, 83:69-76 Havaux M: Spontaneous and thermoinduced photon emission: new methods to detect...
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Báo cáo y học: " Enhanced macrophage tropism of HIV in brain and lymphoid tissues is associated with sensitivity to the broadly neutralizing CD4 binding site antibody b12" ppsx
... neutralization sensitivity to mAb b12 and neutralization sensitivity to sCD4 (p = 0.3279; Additional file 1) These results suggest that macrophage tropism is associated with increased sensitivity to mAb ... entry into macrophages is associated with sensitivity to neutralizing mAb b12 and a broadly neutralizing HIVEnhanced Enhanced HIV entry into macrophages is associated with sensitivity to neutralizing ... neutralization sensitivity to reagents targeting the CD4 binding site and macrophage tropism The capacity of HIV to enter macrophages correlated with neutralization sensitivity to the CD4 bind-...
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Báo cáo y học: " The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu" potx
... to efficient virus release [37], they provide useful analytical tools and were used here to test the sensitivity of BST-2 mutants to Vpu in vitro http://www.retrovirology.com/content/6/1/80 To ... affinity to ConA or DS lectin due to the lack of oligosaccharide modification of this BST-2 mutant Glycosylation of BST-2 is not required for inhibition of virus release and sensitivity to Vpu To assess ... sensitive to Vpu to varying degrees (Fig 6) In particular the C13 and C3A mutants were highly sensitive to Vpu similar to wt BST-2 while expression of the C12 and C23 mutants appeared to be less...
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Báo cáo khoa học: "Activated protein C increases sensitivity to vasoconstriction in rabbit Escherichia coli endotoxin-induced shock" potx
... receptor-dependent endothelium-dependent vasodilator agonist ACh (10-9 to 3.10-5 mmol/l); the receptor-independent endothelium-dependent vasodilator agonist calcium ionophore A23187 (10-9 to 3.10-6 ... compared to the LPS group, suggesting the ability to respond to further endotoxin stimulation In vitro vascular reactivity Vascular contraction The maximal vasoconstrictor response to PE was ... report that aPC is able to prevent endothelial morphological injuries and to increase the sensitivity to the vasoconstrictor agent PE in a well-documented rabbit endotoxin-induced shock model...
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Báo cáo y học: "A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5α" potx
... amplified by PCR from the genomic DNA of infected cells and cloned into pCR 2.1-TOPO vector (Invitrogen, Carlsbad, CA) to generate pTopo-MACAadp42 Nucleotide sequences of independent clones were determined ... respectively To construct the lentivector expressing GFP under the control of cytomegalovirus promoter, we replaced the Eco RI-Apa I fragment corresponding to MA and CA of the pMDLg/p.RRE packaging vector ... HIV-1 to human TRIM5α A G -to- E substitution at the 116th position did not affect the association between CA and CypA or Gag processing To obtain further insight into the effects of the G -to- E...
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