self renewal of embryonic stem cells cell cycle regulation
Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... GFP control cells was not detected When the number of infused cells was raised to × 105 cells, both Sall4A and Sall4B transduced cells repopulated of recipients, whereas GFP transduced cells did ... by washing cells times, then G-CSF (R&D Systems, Minneapolis, MN) was added to cells at a final concentration of 200 ng/ml CFU assay of BM cells Tubes of MethoCult® GF M3434 (Stem Cell Technologies, ... Sall4B expanded cells, × 10 15 WT-like B6.SJL Sall4B expanded cells, × 106 11 WT-like B6.SJL GFP-infected cells, × 106 15 WT-like 11 WT-like B6.SJL Number of injected cells GFP-infected cells, × 10...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...
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differentiation of embryonic stem cells
... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Mouse Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus...
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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx
... promotes self- renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev ... regulators of self- renewal of ES cells It is recently documented that ERK2 null ES cells fail to commit into neural and mesodermal cells [21-24] Similarly, rare brachyury (T) expressed cells were ... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic ... in mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Human embryonic stem cells: theoretical approaches Blastomere biopsy and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which ... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant...
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Elucidation of the physiologic role of TRIP br2 in cell cycle regulation and cancer pathogenesis
... 1.1 Current paradigm of the cell cycle and cancer 25 1.1.1 The general schema of cell cycle progression: simplifying complexity 25 1.1.2 The control of cell cycle progression ... during the cell cycle in U2OS cells 157 Figure 25C TRIP-Br2 is differentially expressed during the cell cycle in COS-7 cells 158 Figure 26A-D Rapid turnover of C-terminal ... 235 Figure 40 CD8+ cytotoxic T cells and CD4+ helper T cells of TRIP-Br2 wildtype and mutant mice (spleen) 236 Figure 41 Proportion of CD4+ memory T cells of TRIP-Br2 wildtype and mutant...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... cells Put together, all these evidence suggest that the regulation of Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cell self renewal mechanism The role of Lefty1 in regulation of ES cell ... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... compare the extent of differentiation between Lefty2 RNAi cells and control cells a) Two seeding densities (300 and 500 cells) were performed for one of the shRNAs while b) 300 cells were replated...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell ... Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have both previously been reported A key objective of this thesis is to reconcile the differences...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/ cm2 without gelatin The next day, the seeded cells were subjected ... Generation of LEFTY2 conditioned medium HEK 293T/17 (ATCC: CRL-11268) cells were seeded in growth medium 24 hours before transfection at a density of 6.28 × 106 cells per 15-cm culture dish The cells...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells ... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self- renewal of teratocarcinoma and embryonic stem cells Oncogene ... signaling fails to maintain self- renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... molecular mechanism underlying self renewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cell self renewal Despite the importance of stem cell self renewal, we are only beginning ... ES cells for the study of pluripotency and pluripotent cell self renewal Scientists have recently reported the successful reprogramming of mouse and human somatic cells into pluripotent stem cells, ... with the regulation of the growth and self renewal of iPS cells rather than the induction of pluripotency per se, as overexpression of Klf4 and c-Myc resulted in formation of tumour cells that...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells ... Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist Criteria met • • • • High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human ... correlated to loss of cell viability upon exposure to low temperature It was observed that some cells detached after exposure to low temperature Virtually all of the detached cells were determined...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... differentiation of mouse embryonic stem cells to hematopoietic cells on an OP9 stromal cell monolayer Methods Enzymol 365, 72–83 21 Strahl BD, Ohba R, Cook RG & Allis CD (1999) Methylation of histone ... fetal liver cells These results indicate acetylation plays a critical role in the transcriptional potentiation and developmental regulation of these genes in progenitor cells or cells that have...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... growing HeLa cells were incubated with nocodazole Cells detached from the bottle by shaking were collected and named mitotic (M) cells Residual cells were collected and named G2 cells These cells and ... transitions in the cell cycle are also required to prevent re-replication of DNA in a single cell cycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7] ... Linearity of DAPI-stained DNA content of a nucleus was preserved throughout such treatments; i.e the DNA content of G2, M cells are almost double that of G1 cells and the DNA content of S cells ranges...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of ... hES cells and adult cells was also significant (P < 0.05) In particular, the expression of miR-519b was 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note,...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation of embryonic stem cells ... role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells...
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