selection of recombinant gpt vaccinia viruses
Báo cáo sinh học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" doc
... respectively [23,24] resulted in viruses of altered plaque properties or narrowed host range, allowing the selection of recombinants after re-introduction of these genes and rescue of the wild-type phenotype ... Expression of a gene of interest without clonal selection In conventional selection procedures using non-viral marker genes and chemicals for selection, clonal purification of the recombinant ... for rapid isolation of the target recombinant, resulting in parental virus-free recombinant stocks in a single round of plaque purification While generation of recombinants of the highly attenuated...
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Báo cáo hóa học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" docx
... respectively [23,24] resulted in viruses of altered plaque properties or narrowed host range, allowing the selection of recombinants after re-introduction of these genes and rescue of the wild-type phenotype ... Expression of a gene of interest without clonal selection In conventional selection procedures using non-viral marker genes and chemicals for selection, clonal purification of the recombinant ... for rapid isolation of the target recombinant, resulting in parental virus-free recombinant stocks in a single round of plaque purification While generation of recombinants of the highly attenuated...
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Báo cáo hóa học: " Isolation and characterization of cidofovir resistant vaccinia viruses" pot
... recombinant viruses Mapping2 Mapping and marker rescue of recombinant viruses A Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant ... characterization of these viruses Our lower drug concentrations indicate that even at low doses of drug the development of resistant viral strains can pose a problem Figure Model of the catalytic core of E9 ... concentrations of CDV and is consistent with results published previously [11] To insure selection of independent mutations, 10 individual CDVR CDVR E9 PCR 13 PCR 14 PCR 15 Figure and marker rescue of recombinant...
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Báo cáo khoa học:" In vitro host range, multiplication and virion forms of recombinant viruses obtained from co-infection in vitro with a vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus isolate" pot
... consists of large double stranded DNA viruses that replicate in the cytoplasm of infected cells [1,2] Within this family, vaccinia and cowpox viruses are members of the genus Orthopoxvirus Poxviruses ... potential of generating novel hybrid viruses that will elucidate our understanding of the biology of recombinant poxviruses, as well as the putative scenarios that might arise following the release of ... some of the factors that might affect the stability of the HA transgene or the loss of the HA expression following serial passage of the transgenic viruses Genome wide mapping of these recombinant...
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Báo cáo khoa học: " In vitro host range, multiplication and virion forms of recombinant viruses obtained from co-infection in vitro with a vaccinia-vectored influenza vaccine and a naturally occurring cowpox virus isolate" pps
... consists of large double stranded DNA viruses that replicate in the cytoplasm of infected cells [1,2] Within this family, vaccinia and cowpox viruses are members of the genus Orthopoxvirus Poxviruses ... potential of generating novel hybrid viruses that will elucidate our understanding of the biology of recombinant poxviruses, as well as the putative scenarios that might arise following the release of ... some of the factors that might affect the stability of the HA transgene or the loss of the HA expression following serial passage of the transgenic viruses Genome wide mapping of these recombinant...
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Detection of bacteria and enteric viruses from river and estuarine sediment
... and visualized by UV illumination Evaluation of recovery rate of preparation method for viruses in sediment As a surrogate of RNA viruses, Sabin strain of Poliovirus type was spiked into the sediment ... AND DISCUSSION Evaluation of recovery rate of preparation method for viruses in sediment Figure shows recovery rates of RNA and DNA viruses Recovery rate (geometric mean) of Poliovirus (RNA virus) ... capsid of approximately 25 nm diameter This virus was also used by Gerba et al (1977) for evaluation of the method Recovery rate was evaluated by dividing amount of spiked Poliovirus by amount of...
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Formation of Aerobic Granular Sludge in a Continuous-Flow Reactor – Control Strategy for the Selection of Well-Settling Granular Sludge
... influencing the selection of well-settling sludge because the sludge selection is one of the most important operational strategies that are essential to successful formation of aerobic granular ... the washout of sludge from the reactor, and therefore, we investigated the effect of surface loading rate and aeration rate on the selection of well-settling sludge and the formation of aerobic ... strategy for the selection of well-settling sludge Then, we applied the strategy to the formation of aerobic granular sludge in a continuous experiment In addition, the effect of feeding pattern,...
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Create pictures worth framing with this selection of tips 3
... keeping your camera steady, invest in some kind of a tripod A good photography tip that can help you is to try taking a lot of different pictures of the same subject This is a great way to help ... common to come from a background of thinking everything has to be even and centered Though the pursuit of perfection maybe a noble one, it is not needed in the realm of photography Capture a more ... picture by having your subject be off to the side of the frame If your camera has an auto-focus feature, it may try to lock onto whatever appears in the middle of the frame Focus manually and lock...
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Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx
... this would not have been of interest in the context of the ongoing projects, or because of the lack of sufficient amounts of purified protein The indications of the yields of expression and reconstitution ... library of prion proteins Table List of plasmids encoding the sequence of the mature cellular form of the prion protein from a variety of species and truncated variants thereof, and of human ... library of prion proteins Table List of plasmids encoding the sequence of the mature cellular form of mPRP (accession number AAA39997) and truncated forms and designed variants thereof, and of mouse...
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Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx
... of E coli in plate assay using 100 pmol of each peptide, complete disappearance of ferroportin signal at the membrane of iron treated macrophage J774 cells in the presence of 700 lM of each of ... irreversible aggregation of 3796 Fig HPLC profile of pure recombinant (A) mouse or (B) human hepcidins (A) Fifty microlitres of huhepc (0.1 mgÆmL)1) was injected on a MOS C8 and (B) 50 lL of m1hepc (0.01 ... microlitres of hepcidin (0.1 mgÆmL)1) were eluted with the following gradient at mLÆmin)1 from 90% of (H2O, 0.1% TFA) and 10% of (H2O 30%, CH3CN 70%, TFA 0.1%) to 10% of (H2O, 0.1% TFA) and 90% of (H2O...
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Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx
... half-reaction of the catalytic cycle Results Expression of recombinant AADH Plasmid pRKAADH was introduced via conjugation into P denitrificans to test for AADH expression The level of recombinant ... native enzyme The migration of both subunits is consistent with the predicted masses of the mature form of the subunits (14 472 and 40 421 Da 5896 Fig Purification of recombinant AADH from P denitrificans ... expected mass of the a subunit to 40 421 Da, which is within error of the measured mass of 40 422 Da Cofactor biogenesis in TTQ-dependent AADH effect; KIE ¼ 4.4) Rate constants for recombinant...
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Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx
... 2004 Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme ... on the result of selection If the cutting sites are in the flexible loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins ... predict the selection result Effect of flexible loops and partially folded intermediate on selection Depending on the position of protease cutting sites in the structure, the existence of flexible...
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Tài liệu Báo cáo khoa học: Unique features of recombinant heme oxygenase of Drosophila melanogaster compared with those of other heme oxygenases studied docx
... the overall structure of DmHO is similar to that of mammalian HO-1 In rat HO-1, His25 works as the proximal ligand of heme iron The His39 residue of DmHO corresponds to His25 of rat HO-1 and therefore ... spectrum of the hemin–DmDHO complex exhibits a highly rhombic, highspin state of hemin, showing pronounced difference from that of the hemin complex of cyanobacterial heme oxygenase isoform-1, ... spin of one of the nitrogen nucleus of an imidazole of a histidine residue trans to the 15NO [16] (Fig 5) The hemin–DmDHO complex in alkaline solution (pH 10.0) does not show the spectrum of a...
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Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf
... detect parses of high quality from the set of parsed sentences To explore the quality of the selected set in terms of filter f-score, we recall that the quality of a selected set of parses increases ... first explore the quality of the selected set in terms of average f-score In Section we reported that the quality of a selected T-set of parses increases as the number of models N increases and ... samples of size S from the training set T R Each sample is selected without replacement Train N copies of the parsing algorithm A, each with one of the samples Parse the test set with each of the...
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot
... availability of recombinant DHODH should expedite discovery of more potent agents for growth control strategies in C albicans, and permit the screening of a large number of compounds, the examination of ... refer to amino-acid residues of the C albicans protein (B) Alignment of the catalytic centre of the recombinantly expressed C albicans DHODH and amino-acid sequences of different DHODH family enzymes ... (classified as family DHODH), the activity of which is independent of ubiquinone and the presence of oxygen [15–17] This feature promotes growth of this yeast Fig Scheme of dihydroorotate dehydrogenase...
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Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf
... treatment of Type diabetes [23] In order to examine the mechanism of action of this class of inhibitors, 3, 5, the stereoisomers of salacinol 6, [25], and the six-membered ring analogues of salacinol ... inhibitor of human pancreatic a-amylase with a reported Ki of 15 nm [33] However the method of action of acarbose is quite complex and it appears to be acting as a type of suicide inhibitor of a-amylase ... treatment of Type II diabetes, was compared to that of salacinol Whereas salacinol at lm concentration inhibited 60– 70% of the breakdown of maltose, lm acarbose only inhibited 4% of the activity...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... no evidence of a gluconeogenic pathway in E histolytica trophozoites [3] In vitro reconstruction of the nal stages of amoebal glycolysis Analysis of the kinetic properties of the recombinant ... characterization of recombinant EhPPDK was as described previously [11] Overexpression and purication of recombinant enzymes One-hundred millilitres of LB (LuriaBertani) [59] containing 30 lgặmL of kanamycin ... rates of activity in typical glycolytic pathways (with ATP-PFK instead of PPi-PFK and PYK instead of PPDK) At pH 6.0, the Vmax values of amoebal PGAM and PPDK showed a slight increase, those of...
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Báo cáo khoa học: "Intelligent Selection of Language Model Training Data" ppt
... in-domain corpus, we chose the English side of the English-French parallel text from release v5 of the Europarl corpus (Koehn, 2005) This consists of proceedings of the European Parliament from 1999 ... tokenization, the sizes of our data sets in terms of sentences and tokens are shown in Table The token counts include added end -of- sentence tokens To implement our data selection method we required ... subsets of the Gigaword data corresponding to cutoff points in the cross-entropy difference scores, and trained 4-gram models (again using absolute discounting with a discount of 0.7) on each of these...
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Báo cáo khoa học: Pressure and heat inactivation of recombinant human acetylcholinesterase Importance of residue E202 for enzyme stability pdf
... charge of proteins [38,39] As observed for ANS binding to BSA, binding of ANS is thought to induce a conformational tightening of the protein by the interplay of ionic and hydrophobic characters of ... Pressure dependence of percentage of residual activity of rHuAChE vs pressure at 10 °C after h of pressure exposure in 10 mM Tris buffer (pH 8.0) Symbols are as in Fig light of electrophoresis ... terms of hydration change of carboxylic residues present in the active site gorge of HuAChE As the X-ray crystal structures of TcAChE [19] and HuAChE [56] revealed, the conserved position of water...
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