selecting and purifying autonomous human variable heavy vh domains

Intake manifold design effect on air fuel mixing and flow for an LPG heavy duty engine

Ngày tải lên : 05/09/2013, 16:30

... engine performance The air-fuel ratio and the specific fuel consumption are measured and determined With the optimized manifold, both AFR and SFC are improved by % and 28 % respectively ISSN 2076-2895 ... 2010 [11] Fuchs T R and C J Rutland Intake Flow Effects on Combustion and Emissions in a Diesel Engine, SAE N° 980508, 1998 [12] Ugur Kesgin, Study on the design of inlet and exhaust system of ... supercharging and filling Lee [8] has developed a computer program to predict the engine performance characteristics through the analysis of the flow in the intake and exhaust systems and of the...

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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Ngày tải lên : 18/02/2014, 14:20

... mediates entry of HSV-1 into human T lymphocytes and trabecular meshwork cells, and is expressed in many fetal and adult human tissues, including the lung, liver, kidney, and lymphoid tissues [7,8,16] ... extensively expressed in human cells of epithelial and neuronal origin [18], whereas nectin-2 is widely expressed in many human tissues, but has only limited expression in neuronal cells and keratinocytes ... receptor 3-OS HS is expressed in multiple human cell lines (e.g neuronal and endothelial cells) and mediates entry of HSV-1, but not HSV-2 [2,13,19] Retinitis and acute retinal necrosis (ARN) caused...

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Tài liệu Báo cáo khoa học: "Different Structures for Evaluating Answers to Complex Questions: Pyramids Won’t Topple, and Neither Will Human Assessors" docx

Ngày tải lên : 20/02/2014, 12:20

... analysis of variance model with the series and run as factors, and the binary F-score as the dependent variable We found signiﬁcant differences between series and between runs (p essentially equal ... thank Kiri and Esther for their kind support References H Dang, J Lin, and D Kelly 2006 Overview of the TREC 2006 question answering track In Proc of TREC 2006 W Hildebrandt, B Katz, and J Lin ... system’s output and V is the set of all binary vital/okay distinction in a straightforward way: vital reference nuggets; wm and wn are the weights of nuggets get a score of one, and okay nuggets...

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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

Ngày tải lên : 21/02/2014, 00:20

... conformation between the receptor activated by cleavage and shedding on the one hand, and the receptor activated by the ligand on the other hand This may be due to different post-translational maturation ... proper folding, trafﬁcking and ligand-mediated signaling but not for ligand binding [37,40] The complete loss in the constitutive activity of the Ó FEBS 2003 Role of cleavage and shedding in TSHR function ... ELISA ([28] and see Methods) on permeabilized cells Figure 2B shows a similar total expression of the wild-type and the truncated b1, b2 and TM409 receptors As shown in Fig 2A, the b1 and b2 mutant...

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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulﬁram potx

Ngày tải lên : 07/03/2014, 02:20

... hydrazines and their N-hydroxylated metabolites [12] NATs plays a key role in the detoxiﬁcation and ⁄ or activation of numerous drugs and carcinogens [13] In humans, two functional isoforms, NAT1 and ... genetics and function of NAT1 and NAT2: role in aromatic amine metabolism and carcinogenesis Mutat Res 65, 506–507 14 Grant DM, Blum M, Beer M & Meyer UA (1991) Monomorphic and polymorphic human ... from Sigma, or Euromedex (Souffelweyersheim, France) Cell culture and exposure to DS Human A549 lung carcinoma cells [43] and human NCIH292 pulmonary mucoepidermoid carcinoma cells [44] were grown...

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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

Ngày tải lên : 07/03/2014, 10:20

... iron–sulfur cluster occurs similarly in mitochondria and in the cytosol To address this question, human IRP1, yeast mitochondrial aconitase (yAco1), and human mitochondrial 1084 aconitase (hAco1) were ... separated by SDS ⁄ PAGE and transferred to a poly(vinylidene fluoride) membrane The band corresponding to m-hIRP1 was cut and its N-terminal sequence was determined From this single band, two N-terminal ... mitochondria and the other one is unknown The protein bands separated from purified aggregates on SDS ⁄ PAGE were cut and identified using MS The results indicated the presence of hIRP1, as expected, in band...

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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

Ngày tải lên : 07/03/2014, 11:20

... screening Error-prone PCR and DNA shuffling were separately used to create a randomized library of protein variants based on the Q143A hMnSOD template One hundred and fifty thousand QC774 transformants ... cloning site of the vector by digestion with NcoI and PstI This vector backbone was excised from an agarose gel and purified For both standard and error-prone PCR amplification of hMnSOD genes, ... grant GM54903 (DS) and National Science Foundation CAREER Award Bes-0348107 (HZ) We thank Patrick Quint and Diane Cabelli for help with kinetics, and Chingkuang Tu for assistance and helpful discussion...

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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

Ngày tải lên : 07/03/2014, 16:20

... expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T-cells bearing Vb1, Vb2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differences in the ... wtSSA–Vb5.2 and SSA(C26S)– Vb5.2 Different concentrations of human Vb2.1 and Vb1 TCRs were also used to measure the binding to the immobilized SSAm and SSA(C26S) Vb1 showed a pattern of association and ... SMEZ1 and 2), three have one Cys (SEI, SEK and SPEC), two have three Cys (SEG and SPEA) and only SSA has more than that, ﬁve Cys As discussed later, the fact that SSA has two Cys residues (Cys26 and...

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Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

Ngày tải lên : 07/03/2014, 16:20

... the enzyme and should be helpful in a comprehensive understanding of the role of hKAT-I, especially in KYNA biosynthesis in the human brain Experimental procedures Enzyme expression and purification ... first strand human liver cDNA (Clontech, Palo Alto, CA, USA) using a specific forward (5¢-CTCGAGATGGCCAAACAGCTG CAG) and reverse primer (5-AAGCTTAGAGTTCCAC CTTCCACTT) containing a XhoI and a HindIII ... phenylalanine, tryptophan and tyrosine); sulfur containing amino acids (including, methionine and cysteine) and other aliphatic amino acids (including, glutamine, leucine, histidine, and aminobutyrate)...

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Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

Ngày tải lên : 08/03/2014, 08:20

... in both human and Xenopus lysates, which recognises the PR65 a and b isoforms equally well The best anti-C monoclonal was found to be F2 6A10, and it recognizes PP2AC in rabbit, mouse and Xenopus ... 2a and exon in the human PR130 Ó FEBS 2003 382 I Stevens et al (Eur J Biochem 270) Table Exon-intron organization of the human PR72/PR130 gene Exon sequences are given in capitals Human PR130 and ... (Promega) and the appropriate RNA polymerase (T3 and T7) The translation products were subjected to SDS/PAGE (12%, w/v) and visualised by autoradiography Sequence analysis Sequence comparisons and...

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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

Ngày tải lên : 08/03/2014, 10:20

... studied, indicated that their effects upon Hb A0 and cross-linked Hb differ (Figs and vs Figs and 2) This difference in behavior of native human Hb A0 and cross-linked Hb, in the presence of the LPSs ... oxidation and denaturation Methods Enzymol 231, 480–490 18 Rifkind, J.M., Lauer, L.D., Chiang, S.C & Li, N.C (1976) Copper and the oxidation of hemoglobin: a comparison of horse and human hemoglobins ... effects of rough and smooth LPSs of E coli, both in the presence and absence of EDTA (Fig 1) The rate of oxidation was increased in the presence of the LPSs of both the smooth and rough E coli,...

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Nervous system and sensory organs color atlas and textbook of human anatomy potx

Ngày tải lên : 15/03/2014, 19:20

... also runs through the human body and innervates all intestinal organs, blood vessels, and glands It is called the autonomic (visceral, or vegetative) nervous system (ANS), and consists of two components ... various shapes (short and plump in the perikaryon, long and slender in the dendrites and the axon) and move constantly along fixed cytoplasmic paths between the Kahle, Color Atlas of Human Anatomy, ... vesicle, and the pituitary Kahle, Color Atlas of Human Anatomy, Vol © 2003 Thieme All rights reserved Usage subject to terms and conditions of license Development of the Brain A – D The brain in human...

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$Effect of metal spiking on different chemical pools and chemically extractable fractions of heavy metals$

Effect of metal spiking on different chemical pools and chemically extractable fractions of heavy metals

Ngày tải lên : 16/03/2014, 00:07

... the F3 and F5 fractions of heavy metals could explain the lower concentration of heavy metals in F2 and F1 fractions [19] This might be ascribed to the presence of unoxidized organic matter and ... G Kandpal et al / Journal of Hazardous Materials 106B (2004) 133–137 135 Table The contents of heavy metals in different chemical pools, DTPA And M CaCl2 extracts in sewage sludge (SS) and heavy ... **, * and NS indicate signiﬁcance at P = 0.01, 0.05 and non-signiﬁcant difference between pairs, respectively 3.3 Effect of metal spiking and incubation on chemical pools Heavy metal spiking and...

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Research " DETERMINANTS OF THE EARNINGS GAP BETWEEN BLACKS AND WHITES: A HUMAN CAPITAL APPROACH " ppt

Ngày tải lên : 16/03/2014, 03:20

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Báo cáo khoa học: The propeptide of cruzipain ) a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F ppt

Ngày tải lên : 16/03/2014, 11:20

... a PBL residue, displayed as a ball -and- stick model and highlighted in the sequence alignment and cathepsin F than in the cathepsin V, L, K and S proregions, and thus they may represent another ... from as previously described [37] Human cathepsin B and a 0.8% agarose gel, digested with BamHI and SmaI, cloned human cathepsin S were purchased from Sigma and Calbiointo the respective sites ... to macrophages Hela cells and androgen-independent DU145 prostate cancer cells derived from brain metastasis of human prostate cancer were obtained from the ATCC, and human primary cultures of...

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Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot

Ngày tải lên : 16/03/2014, 14:20

... n.d n.d and pCH2pppA appear to be the strongest inhibitors of both the human and plant enzymes The Ki values estimated for the human enzyme, 1.6 and 2.3 nm, respectively, were over 30- and 20-fold ... analogue) and the investigated enzyme For the yellow lupin p4A hydrolase the mixture contained Hepes ⁄ KOH buffer (pH 8.2) and mm MgCl2, for the human enzyme Hepes ⁄ KOH (pH 7.0) and mm CoCl2, and ... asymmetrically acting ones (EC 3.6.1.17), from human [35] and from narrow-leaved lupin (Lupinus angustifolius) [36] that split Ap4A into ATP and AMP, and the Co2+-dependent symmetrically acting...

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A study on English idioms and proverbs expressing human feelings and emotions

Ngày tải lên : 20/03/2014, 01:26

... idioms and proverbs 4.Role of idioms and proverbs in life CHAPTER II: ENGLISH IDIOMS AND PROVERBS EXPRESSING HUMAN FEELINGS AND EMOTIONS 2.1.Overview on English idioms and proverbs expressing human ... and proverbs in communication, especially expressing my feelings and emotions as well as understanding the other people „s feelings and emotions in life, which helps human beings understand and ... study: Idioms and proverbs relating to human feelings and emotions are raried ,in this paper, only some typical idioms and proverbs on human feelings and emotions are presented and analyzed Particularly...

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Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc

Ngày tải lên : 22/03/2014, 21:20

... (SV40LT) and adenovirus E1A (AdE1A) also target the Rb protein and share sequence and functional conservation with the HPV E7 protein [21,22] E7, AdE1A and SV40LT each contain several functional and ... motif) and yellow (FxxxV motif) Dark grey, interactions mediated by globular domains; light grey, interactions at unknown sites Structural domains are indicated above each cartoon, and the Rb domains ... [70] and manual curation Sequences were obtained from PFAM and ENSEMBL [71,72] and from a Psi-Blast search of UNIPROT [73] using the human Rb protein as seed (UniProt ID: P06400) UniProt and Ensembl...

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Manhood Of Humanity The Science and Art of Human Engineering potx

Ngày tải lên : 22/03/2014, 23:20

... characteristic autonomous activities: Autonomous change, Autonomous excretion, Autonomous ingestion, Autonomous assimilation, Autonomous growth, Autonomous movement, Autonomous multiplication, Autonomous ... propensities and powers that make human beings human and not animal When and only when this fact is clearly seen and [pg 063]keenly realized, there will begin the science of man—the science and art of human ... Why the disease? And why no remedy at hand? The answer is the same for both And the answer is that the so-called sciences of ethics and jurisprudence and economics and politics and government have...

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SECOND EDITION Toxicology and Environmental Hazards HUMAN HEALTH Ecosystems docx

Ngày tải lên : 23/03/2014, 01:20

... EDITION Ecosystems and HUMAN HEALTH Toxicology and Environmental Hazards L1568_ FM_fm Page Thursday, May 10, 2001 4:00 PM SECOND EDITION Ecosystems and HUMAN HEALTH Toxicology and Environmental ... reactions include N- and O-dealkylations, side-chain and aromatic hydroxylations, N-oxidation and hydroxylation, sulfoxide formation, and desulfuration Hydrolysis of esters and amides also occurs ... Thursday, May 10, 2001 4:07 PM 10 Ecosystems and human health: toxicology and environmental hazards An important concept in understanding toxication and detoxication of xenobiotics is enzyme induction...

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