sampling electrophoresis and biosensor analysis of cytoplasm for subcellular biochemical assays

Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt

Ngày tải lên : 07/08/2014, 17:23

... for 12 hrs, 500 V for h, 1,000 V for h and 4,500 V for 10 hrs, having a total of 46,5 kVh, (B) In gel rehydration conditions were at V for hrs followed by 30 V for hrs, 200 V for hrs, 500 V for ... molecules of 16/17, 29, 37, 46 kDa Two-dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 143 Fig Analysis of 2-DE and immunoblot profiles of N caninum ... optimization of sample preparation and IEF condition for 2-DE and analysis of 2DE profiles Results collected allowed to manifest the usefulness of 2-DE combined with immunoblotting in defining proteins and...

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Báo cáo y học: "ptimized design and data analysis of tag-based cytosine methylation assays" pps

Ngày tải lên : 09/08/2014, 20:21

... 10 of 11 reduced representation bisulfite sequencing; UCSC: University of California Santa Cruz Authors' contributions MS and JMG designed the assays and strategies for its analysis, MS performed ... cycles of 95°C for 30 seconds, primer-specific Tm for 30 seconds and 72°C for minute, followed by 72°C for 10 minutes for the final extension Primer-specific Tm and sequence information are provided ... MSCC [15] and HELP-seq [10] assays, not only by means of technical modifications such as the use of EcoP15I but also because of the concurrent use of MspI for normalization The effect of these...

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Tài liệu tools for institutional political and social analysis of policy reform pptx

Ngày tải lên : 23/02/2014, 21:20

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tools for institutional political and social analysis of policy reform docx

Ngày tải lên : 09/03/2014, 22:20

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The Report of the Task Force on Financial Mechanisms for ICT for Development - A review of trends and an analysis of gaps and promising practices ppt

Ngày tải lên : 15/03/2014, 19:20

... development of Rwanda towards an information and knowledge based economy The emerging information revolution offers Rwanda a window of opportunity to leap-frog the stage of industrialisation and transform ... goals for expanding financial resources available for ICT development, therefore, not necessarily amount to a trade-off with financing for more direct and urgent forms of support, such as for food, ... Force’s best understanding of the broad and constantly changing scope of the ICT sector and the use of ICT in the developing world from a financing and development perspective In the report of...

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Báo cáo khoa học: "A GENERATIVE GRAMMAR APPROACH FOR THE MORPHOLOGIC AND MORPHOSYNTACTIC ANALYSIS OF ITALIAN" ppt

Ngày tải lên : 24/03/2014, 05:21

... different classes of endings: andare (to go), for example, admits two different stems, vad (go) and and (went); so there exist two subclasses of endings named respectively dvl andare and -> verbalization ... performed: I the analysis can be performed completely the lemma can neither be altered nor affixed (this is the case for example of prepositions and conjunctions) only the longest analysis of ... regular structures such as compound tenses of verbs and comparative and superlative forms of adjectives I g~ lo Compound tenses of verbs are described by means of a regular grammar, whose rules are...

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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

Ngày tải lên : 19/06/2014, 08:20

... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... PCR conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni R: ... and one to contain a dual infection with EACMV and ACMV (Fig 4c) Use of FTA technology for sampling, retrieval and analysis of RNA viruses Since RNA viruses are responsible for the majority of...

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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

Ngày tải lên : 20/06/2014, 04:20

... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... PCR conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni R: ... and one to contain a dual infection with EACMV and ACMV (Fig 4c) Use of FTA technology for sampling, retrieval and analysis of RNA viruses Since RNA viruses are responsible for the majority of...

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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx

Ngày tải lên : 20/06/2014, 08:20

... Ultimately, the frequent development of drug resistance and the lack of alternatives for the treatment of drug resistant disease are responsible for a 5-year survival of approximately 30% in ovarian ... incubation; 95°C, 10 for AmpliTaq Gold activation; and 40 cycles of melting (95°C, 15 sec) and annealing/extension (60°C for min) PCR reactions for each template were performed in duplicate in ... Inc.), washing and scanning were performed according to the Illumina BeadStation 5006 manual (revision C) Array data processing and analysis was performed using Illumina Bead Studio software Hierarchical...

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Báo cáo hóa học: " Research Article Design and Performance Analysis of an Adaptive Receiver for Multicarrier DS-CDMA" doc

Ngày tải lên : 22/06/2014, 19:20

... instead of simply implementing a heuristic algorithm, we perform a thorough analysis on the system performance and obtain a simple closed form expression for the bit-error rate (BER) of the APIC ... waveform with unit amplitude and duration τ, ωq and φq are the frequency and random phase of the qth subcarrier, respectively, and c(k) (t) is the spreading sequence of user k, which is given by ... second moment and βq (t) is uniformly distributed (k) over and 2π It is assumed that the channel gain ζq (t) is independent and identically distributed (i.i.d.) for diﬀerent values of k and q This...

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Báo cáo y học: " Assessment and histological analysis of the IPRL technique for sequential in situ liver biopsy" ppsx

Ngày tải lên : 13/08/2014, 13:20

... which were weighed and recorded The central third was typically used for histology, and if required, the lateral thirds can be homogenised for biochemical assays For the duration of each IPRL experiment, ... Centre for Microscopy & Microanalysis, The University of Sydney Author details Faculty of Pharmacy, University of Sydney, NSW 2006, Australia 2Australian Centre for Microscopy & Microanalysis, ... cytoplasm of most hepatocytes is pale and eosinophilic with finely granular basophilic inclusions The hepatic sinusoids and central veins are predominantly clear of erythrocytes Fifteen out of...

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Báo cáo y học: "Base relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" potx

Ngày tải lên : 14/08/2014, 17:22

... 28.95 Volume 8, Issue 2, Article R19 comment Sample Standard1 Standard1 Standard2 Standard2 Standard3 Standard3 Standard4 Standard4 Standard5 Standard5 Genome Biology 2007, R19.4 Genome Biology 2007, ... under the form of XML files [15] Data can be imported from a number of data formats Two standards (qBase internal format and RDML (Real-time PCR Data Markup Language)) and a number of instrument ... used in formulas N, number of replicates i; g, number of genes j; c, number of IRCs m, m'; r, number of runs l, l'; s, number of samples k; f, number of reference genes p, p'; h, number of standard...

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Báo cáo sinh học: "Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection" pptx

Ngày tải lên : 14/08/2014, 19:22

... counting, and cytotoxicity assays CB also performed the qPCR, and drafted significant portions of the manuscript KR co-performed all nucleofections, microscopy, cell counting, cytotoxicity assays, and ... nucleofection conditions for A549 cells, define the minimal assays needed for the evaluation of 96-well shuttle results, and deliver an siRNA targeting complex through nucleofection in a 96-well format ... point was first standardized by subtracting the mean of the data set from each data point and then dividing by the standard deviation of the data set Standardized and raw data for the secondary...

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Development of meshfree methods for three dimensional and adaptive analysis of solid mechanics problems

Ngày tải lên : 14/09/2015, 08:39

... weak-strong (MWS) form method, which is formulated based on the combination of weak form and strong form The key idea of the MWS method is that both the strong form and local weak form are used for establishing ... weak form and local weak form The PIM has also been formulated in meshfree weak-strong (MWS) form, in which the method is formulated based on the combination of strong and local weak forms (Liu and ... when weak form was used in the formulation Since then, more and more research efforts were devoted to the study of meshfree methods and a group of meshfree methods have been proposed and developed...

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Design and performance analysis of quadratic form receivers for fading channels

Ngày tải lên : 14/09/2015, 11:19

... design and performance analysis of QFRs In the following, a literature review of QFRs and some related topics will be given 1.2 Review of Quadratic-Form Receivers and Related Topics The quadratic-form ... 2.4 Error Performance Analysis for USTM After arriving at the QR and the CR for USTM, Hochwald and Marzetta derived in [21] the expressions of PEP and CUB for both the receivers For the special ... investigating the performance of the QR and developing some GQRs for USTM to improve its error performance In [21], Hochwald and Marzetta analyzed the error performance of the QR for USTM The pairwise...

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Design and analysis of algorithms for solving a class of routing shop scheduling problems

Ngày tải lên : 14/09/2015, 14:07

... DESIGN AND ANALYSIS OF ALGORITHMS FOR SOLVING A CLASS OF ROUTING SHOP SCHEDULING PROBLEMS LIU SHUBIN (M.Eng NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF INDUSTRIAL ... The objectives of scheduling problems are divided into two classes, namely, regular measures of performance and non-regular measures of performance For the regular measures of performance, the ... disjunctive graph formulation of the OSSP Two dispatching rule based heuristics were developed by Guéret and Prins (1998) and the performance of the two heuristics was evaluated based on both randomly...

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Design and analysis of algorithms for solving some stochastic vehicle routing and scheduling problems

Ngày tải lên : 17/09/2015, 17:19

... Scope and Purpose of this Study……………………………………………….6 1.3.1 Scope and Purpose of Part I of this Study………………………………6 1.3.2 Scope and Purpose of Part II of this Study………………………………7 1.4 Structure of the ... of random variables corresponding to travel and service time θ (ξ k ) Total travel and service time of the route in excess of T when the realization of the random variable is ξ k ξk t ij A random ... xii LIST OF TABLES 3.1 Average performance with the increase of problem size (Demands follow uniform distribution U[0, 20]) 3.2 Average performance with the increase of demand mean and variance...

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MODELLING AND STRESS ANALYSIS OF COLUMN BRACKET FOR ROTARY JIB CRANE

Ngày tải lên : 07/10/2016, 00:03

... Model of Column bracket Figure.10: Thee-D Model for analysis of Column bracket The analysis, the Solid Works and COSMOS is used the analysis is carried out in two load steps 6.1 Analysis results of ... 3-D Model of Column Bracket are created for further analysis The purpose of analysis is to insure safety of the bracket and supporting structure Sustained loads are by using self weight and operating ... displacement analysis of Isection Boom for 20 KN loading condition The purpose of static analysis is to insure safety of the boom and supporting structure Sustained loads are by using self weight and...

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Static and Dynamic Analysis of Space frames

Ngày tải lên : 06/09/2012, 15:18

... installation and the single installation of RM2000 and GP2000 Please always uninstall old program versions before installing a new program release! 1.2 Unit dependency of Import/Export of RM2000 Export and ... 8.49.01 • • • Actions for diagram plot of internal forces, deformations, stresses and reinforcement amount added Additionally option “Diagram” added to the “Result/Load case” and “Result/Superposition” ... to choose between E- and Fformat of the written values Information about units added to the plot file of the action PlInfl, screen plot Results/ PlInfl and to the list file of the action DoList/Influence...

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Static and Dynamic Analysis of Spaceframes

Ngày tải lên : 06/09/2012, 15:55

... Disclaimer and Copyright Disclaimer Much time and effort have gone into the development and documentation of RM2000 and GP2000 The programs have been thoroughly tested and used The user accepts and ... Supposition: Main Girder Axis and segment, Cross Section for the girder plus the cross section for the piers plus the Segment numbering and assignment and Part numbering and assignment for the main girder ... segment De-select: Conn to “node for part” for node and enter ‘1111’ for the end node number for node Repeat the above procedure for the LH Bearing Element (number 1103) and the ground connection (number1100)...

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