sampling assays and sample analyses
Báo cáo y học: " Sampling and sensitivity analyses tools (SaSAT) for computational modelling" docx
... factorial sampling, and Latin Hypercube Sampling Random sampling The first obvious sampling approach is random sampling whereby each parameter's distribution is used to draw N values randomly This ... different sampling schemes Examples of the three different sampling schemes: (a) random sampling, (b) full factorial sampling, and (c) Latin Hypercube Sampling, for a simple case of 10 samples (samples ... (6,10) and λ ~ N (0.4, 0.1) are shown) In random sampling, there are regions of the parameter space that are not sampled and other regions that are heavily sampled; in full factorial sampling, a random...
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MECHANISTIC CHARACTERISATION OF DENGUE VIRUS RNA DEPENDENT RNA POLYMERASE NON NUCLEOSIDE INHIBITOR BINDING POCKET THROUGH IN VITRO BIOCHEMICAL ASSAYS AND REVERSE GENETICS ANALYSES
... membrane protein and envelope) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) (Figure 1.5 C) The conserved 5´ and 3´ UTRs are made up of ~100 and 400 nucleotides ... Non-Nucleoside Inhibitor Binding Pocket through In Vitro Biochemical Assays and Reverse Genetics Analyses" is my original work and it has been written by me in its entirety I have duly acknowledged ... would like to express my profound thanks and love to my wonderful Husband, Patrick Kwasi Otoo who is always there for me and whose love, care, companionship and motivation propelled me to have a...
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Exergoeconomic performance optimization of an endoreversible intercooled regenerated Brayton cogeneration plant Part 1: Thermodynamic model and parameter analyses
... thermal capacity rate (mass flow rate and specific heat product) Cwf The hot-, cold- and consumer-side heat reservoir temperatures are TH , TL and TK respectively, and the intercooling fluid temperature ... fluid and the heat reservoirs, the regenerator and the intercooler are counter-flow The conductances (heat transfer surface area and heat transfer coefficient product) of the hot-, cold- and consumer-side ... decreases, and π Π max increases first, and then decreases, but the value of π Π max changes slightly Figure Characteristics of Π max and (ηex )Π versus τ max Figure 10 Characteristics of π Π max and...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... interactions: binding and cleavage by ribonuclease A and variants at Lys7, Arg10, and Lys66 Biochemistry 37, 1212112132 31 Anderson DG, Hammes GG & Walz FG Jr (1968) Binding of phosphate ligands to ribonuclease ... RiIi(h), where Ii(h) and I(h) are the ith and the mean measurements of the intensity of reection h, respectively Rcryst = Rh |Fo ) Fc| RhFo, where Fo and Fc are the observed and calculated structure ... dashed lines, and water molecules are in cyan (C, D) Stereo pictures of 2Fo ) Fc contoured at 1.0r for N-acylsulfonamide and N-acylsulfonamide 6, respectively N-Acylsulfonamide (2Â-deoxy) and N-acylsulfonamide...
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Tài liệu Báo cáo khoa học: "Toward finer-grained sentiment identification in product reviews through linguistic and ontological analyses" ppt
... ‘thickness’ Hu and Liu (2004) identified attributes of IT products based on frequent noun phrases and Popescu and Etzioni (2005) utilized PMI values between product class (hotels and scanners) and some ... quality element classification (Huiskonen and Pirttila, 1998), the conceptual granularities, and such syntactic and lexical clues as conjunction items and adverbs They may play significant roles ... significant differences between ‘likeness’ and ‘contrary’ (p = 015), and between ‘likeness’ and ‘contrary with contrastive markers’ (p = 025) The ‘contrary’ and ‘contrary with contrastive markers’...
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
... SDS/PAGE of wild type and H273A mutant enzymes and their individual subunits Samples were applied to a 12.5% gel, and then stained with Coomassie Brilliant Blue R250 Puried wild type and H273A mutant ... subunits of the H273A mutant Fig Phosphorylation and dephosphorylation of Cl-ACL and the H273A mutant protein Samples were subjected to SDS/PAGE (12.5%) and autoradiography Lane 1, puried wild type ... onto lanes and 2, respectively The subunits AclA and AclB of wild type and H273A Cl-ACL were dissociated by hydroxyapatite column chromatography AclB eluted with 10100 mM KPB (lanes and 6), while...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... cocrystallization and soaking methods, respectively, and determined their tertiary structures We collected X-ray diffraction data for the AD2 E526A and D524A mutants and refined them to ˚ resolutions of 1.80 and ... Bank code 2HVM and 1LLO) (B), ScCTS1 (Protein Data Bank code 2UY2 and 2UY4) (C), SmChiB (Protein Data Bank code 1E15 and 1E6N) (D), CcCTS1 (Protein Data Bank code 1D2K and 1LL4) (E) and BcChiA1 ... diagram is the superimposition of ligand (substrate or inhibitor)-free and ligand-bound structures In (F), only the ligand-free structure is shown because no ligand-bound structure is available The...
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Blank values, adsorption, pre concentration, and sample preservation for arsenic speciation of environme
... i.d.) and a 200-l injection loop (Stainless Steel), and a LC autosampler 465 (Kontron Instruments, Germany) was connected to an anion-exchange column (IonPak AG7 and AS7, both Dionex) and coupled ... Soil-pore water sampled from forest floors contains higher concentrations of cations, anions, and DOC as compared to that sampled from mineral soil [19] The sampled rainwater and soil-pore water ... immediately after sampling For this, we used an amount of each g As l−1 and 1.25 mM EDTA, and incubated the samples in the dark at 20 ± ◦ C for seven days A set of control samples were spiked...
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Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx
... scutellatus: OS2 AAB33760; P australis: PA11 and PA13, P04056 and P04057; P porphyriacus: pseudexin A and B, P20258 and P20259; and O hannah: acidic I and II, P80966 and Q9DF33 cobra venom group IB PLA ... (3FTx-LT and 3FTx-RK), but two of their 3FTx show sequence and mass variations (Table 1) In particular, the major 3FTx-LI (-LK) in sample KBf and 3FTx-LF in sample KBf were very different PLAs and ... type I a-neurotoxin and orphan groups II, III, and XVIII In contrast, venoms of B multicinctus, B candidus and B flaviceps have special type II a- and j-neurotoxins [40,48] and orphan groups IV,...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... purification of a-PEC and the problems arising during analyses of the protein at high concentrations The present study describes a very effective method of isolation and purification of PEC and its photoactive ... Crystallization and preliminary X-ray experiments suggest pronounced conformational alterations of both the protein and the PVB chromophore, depending on light quality N-Terminal amino acid analyses and ... spectrometry and N-terminal amino acid sequencing In both its E- and Z-configurations, peptide was characterized further by NMR spectroscopy peptide charges because bands with nearly the same charge (bands...
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Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot
... 2HK7, 2HK8 and 2NLO), (b) binary complex bound with either cofactor (1NPD, 1NVT, 1NYT, 1O9B, 1P77, 1VI2 and 2CY0) or substrate (2D5C, 2GPT and 2O7Q) and (c) inactive (2HK9_A and 2EV9) and active ... central six-strand mixed b sheet (b2, b1, b3, b5, b6 and b4; b5 is antiparallel to the others) flanked by three a-helices (a1, a9 and a8) on the inner side and by two a-helices (a2 and a3) and two 310 ... thaliana (2GPT [14], 2O7Q and 2O7S [15]) and Geobacillus kaustophilus (2EGG); YdiB from E coli (1O9B [9], 1NPD [16] and 1VI2) and Corynebacterium glutamicum (2NLO [17]); and SDHL from H influenzae...
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Báo cáo khoa học: Structural and mutational analyses of protein–protein interactions between transthyretin and retinol-binding protein doc
... b-strands H and F To form the tetramer, two dimers associate back to back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H ... b-strand of RBP and the hyper-variable regions of Fab: loops 53–56 and 100–103 and the short helix 28–32 of chain H and loops 31–36 and 53–56 of chain L The interactions, which are mainly polar and ... group is almost solvent exposed, in the region of the loops that connect b-strands A and B, C and D and E and F and surround the entrance of the b-barrel at the open end of the cavity As a result...
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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot
... 484.5 Band 864.9 133.0 Band 100.0 100.0 Band 412.2 14.3 Band 6+7 20.9 89.9 Total Band Monomeric Lhc 50.5 Band Band Free pigments 37.4 2046.7 872.2 Band LHCII trimer C B6 B7 WT Zb63 WT Zb63 Band ... proteins (CP26, CP29 and LHCII monomers) (band 2), and tri4620 meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores Bands and contained supramolecular complexes of PSII with ... spectroscopy and SDS ⁄ PAGE and immunoblotting (Fig 3C) For the viridis zb63 mutant, the respective bands contained: free pigments (band 1), monomeric antenna proteins (CP26, CP29 and LHCII monomers) (band...
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nanochromatography and nanocapillary electrophoresis. pharmaceutical and environmental analyses, 2009, p.282
... having the holes and serving as reservoirs with volumes of approximately 150 mL each The channels between the sample reservoir and sample waste reservoir were used for sampling and the channels ... 2.6a to c) for sample solution and organic solvents (Fig 2.6b and c) were mm long, 25 mm deep, and 150 mm wide The extraction channel (Fig 2.6c and d) was 10 mm long, 25 mm deep, and 250 mm wide ... instrumentation, detection, sample preparation, and applications of nanoliquid chromatography and nanocapillary electrophoresis techniques for analyses at the nanogram level This book describes analyses at the...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... picogram level Figure and FTAsymptoms on field Disease 1sampling method grown cassava and maize plants Disease symptoms on field grown cassava and maize plants and FTA sampling method (a) cassava ... generating the knowledge and improved germplasm required by scientists, breeders and farmers to combat these diseases and maximize crop yields Effective methods for sampling, storage and retrieval of ... cassava mosaic disease (CMD) and Maize streak virus (MSV) (Fig 1a and 1b) were pressed onto FTA cards in Malawi and Western Kenya Samples were returned to the DDPSC and processed as described above...
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Báo cáo hóa học: " Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses" pptx
... contributions MAB, VS, and EM co-conceived, designed and coordinated the study, participated in the molecular studies, sequence alignment, phylogenetic and genetic analyses, interpreted data, and co-drafted ... fragments, and includes the complete coding region and partial sequences from both 5'UTR and 3'UTR A codonbased nucleotide alignment of the coding region of the new sequence was used in phylogenetic analyses, ... different regions: cases from Egypt (5'UTR, core and NS5B), cases also from Egypt (5'UTR, core/E1 and NS5B) and cases from Canada (5'UTR, core/E1 and NS5B) In all cases, we observed phylogenetic...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... picogram level Figure and FTAsymptoms on field Disease 1sampling method grown cassava and maize plants Disease symptoms on field grown cassava and maize plants and FTA sampling method (a) cassava ... generating the knowledge and improved germplasm required by scientists, breeders and farmers to combat these diseases and maximize crop yields Effective methods for sampling, storage and retrieval of ... cassava mosaic disease (CMD) and Maize streak virus (MSV) (Fig 1a and 1b) were pressed onto FTA cards in Malawi and Western Kenya Samples were returned to the DDPSC and processed as described above...
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Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility" doc
... Ritland, 1986b), improve seed yields (Webber, 1987) and reduce the negative impact of selfing and contamination (El-Kassaby and Ritland, 1986b; Wheeler and Jech, 1986b) However, success of SMP is dependent ... orchard populations is a constant concern and estimated levels of contamination range from 6-56% (Smith and Adams, 1983; El-Kassaby and Ritland, 1986a; Wheeler and Jech, least, comparable fertility ... pollination technique was used and individual clones were the experimental unit and cones were the sampling unit Linear and non-linear rea pollen lot (defined gression, analyses were completed on...
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báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf
... root and COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly suppressed COPT4 in root and induced COPT5, COPT6, and ... functional complementation and gene expression analyses and drafted the manuscript XL and JX provided biochemical and molecular analysis supports SW contributed to data interpretation and to writing the ... p416GPD-COPT6 and grown to log phase on SCHis-Ura The GFP signal and Nomarski optical images were observed using a fluorescence microscopy the Fet3 and Fet4 proteins and was defective in both low- and...
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báo cáo khoa học: " ''''Who''''s who'''' in two different flower types of Calluna vulgaris (Ericaceae): morphological and molecular analyses of flower organ identity" pdf
... fused and resemble the whorl I organs in shape, color and stability SEM of the abaxial and adaxial epidermis structures (n = varieties each) of whorl I and whorl II organs in both the wild-type and ... molecular methods and the experiments, performed the sequence alignments and all other genetic and molecular data analysis, captured the macroscopic, histological and SEM images and drafted the ... C vulgaris and carried out the complete histological analyses JF established and carried out the flowcytometric methods and experiments AH participated in the experimental design and critically...
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