saccharomyces cerevisiae glucan polysaccharides occurrence separation and application in food feed and health industries
Báo cáo y học: "Spore germination in Saccharomyces cerevisiae: global gene expression patterns and cell cycle landmarks" potx
Ngày tải lên :
14/08/2014, 08:20
... germination in rich medium (YPD) Germination of spores preincubated in glucose Germination of spores preincubated in ‘nitrogen’ Germination transcription program in spores pre-incubated in glucose ... function CSI2 Protein involved in chitin synthesis MSH2 Component with Msh3p and Msh6p of DNA mismatch binding factor, involved in repair of single base mismatches and short insertions/deletions ... the incubation in glucose Consistent with this, genes involved in translation (rRNA processing and protein synthesis genes in Figure 6), which are induced by glucose with the same initial kinetics...
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Lecithin overview, application in food technology
Ngày tải lên :
27/10/2016, 22:42
... icings, frostings, instant beverage mixes, instant foods, margarines, and release agents Purified Lecithin Yelkin Gold Baked goods, confections, instantized foods, instant beverage mixes, and ... from lecithin, leaving it dry and easy to mix Its outstanding quality and purity has no off-flavors or odors, so it maintains the integrity of instant food products Ultralec is available in powdered, ... Lecithins Yelkin® lecithin: A series of standardized lecithins that helps retain moisture and emulsify Ultralec® lecithin: ADM’s exclusive, ultrafiltered, deoiled lecithin is used in hydrophilic instantizing...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
Ngày tải lên :
18/02/2014, 13:20
... kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae Mol Biol Cell 15, 5329–5345 15 Versele M & Thorner J (2004) Septin collar formation in budding yeast requires GTP binding and ... mating and filamentous growth was normal in cells lacking ARE1 and ARE2 (data not shown), but apical bud growth following G1 cyclin overexpression was affected (Fig 5) During budding, the cyclin-dependent ... strain show here that Ste20p forms a complex with Are1p and Are2p The functional link behind this finding is not clear Interestingly, Ste20p and Cla4p also down-regulate sterol uptake by inhibiting...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... suggesting that an open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP1A1 requiring N-terminal truncation, we have shown that intact ... ferredoxin and ferrodoxin reductase [27,28] Results Expression of intact and truncated rat CYPs in Saccharomyces cerevisiae The levels of expression of various CYP cDNAs were measured by resolving ... studied using a combination of treatment with trypsin, treatment with digitonin plus trypsin, and extraction with alkaline Na2CO3 + ⁄ 1A1, + 33 ⁄ 1A1, and TIM23, which was used as an internal...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
Ngày tải lên :
19/02/2014, 12:20
... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine-binding ... participate in the metabolism of nucleotides In this context, the assertion that pyridoxine may be the precursor of the pyrimidine moiety of thiamin in yeast is intriguing [27] In fungi, pyroA and SOR1 ... Srinivasan, M., Pochart, P et al (2000) A comprehensive analysis of protein– protein interactions in Saccharomyces cerevisiae Nature 403, 623–627 24 Mei, B & Zalkin, H (1989) A cysteine-histidine-aspartate...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
Ngày tải lên :
20/02/2014, 01:20
... intermediates involved in NAD biosynthesis (nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, nicotinic acid and quinolinic acid) did not inhibit Yef1p (Table 2) HgCl2 inhibited enzyme ... 1.9 4.0 3.1 2.7 4.9 a This strain was grown in media lacking leucine The other strains were grown in media containing leucine Complementing abilities of LEU2, UTR1 and YEF1 for the growth defects ... upstream and downstream of POS5 was obtained using primers pos5hisf and pos5hisr and was introduced into MK424 (utr1) and MK426 (yef1), yielding MK803 (utr1pos5) and MK804 (yef1pos5) CgLEU2 flanking...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
Ngày tải lên :
20/02/2014, 01:20
... Processing of pre-ribosomal RNA in Saccharomyces cerevisiae Yeast 11, 1629–1650 Kressler D, Linder P & Cruz J (1999) Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae ... [32P]5.8S incubated with increasing amounts of bovine serum albumin; 11, 20 pmol of bovine serum albumin and 40 pmol of cold 5.8S rRNA; 12, [32P]5.8S, 20 pmol bovine serum albumin and 40 pmol ... with the core proteins Cbf5p, Gar1p, Nhp2p and Nop10p and function in the conversion of uridine into pseudouridine [17–23] In addition to the core snoRNP proteins, other proteins have been found...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
Ngày tải lên :
07/03/2014, 04:20
... decrease in O2 concentration in both the gas and liquid phases and the rapid increase in CO2 concentration in both phases following the shift in benzoic acid concentration reflect a rapid increase in ... process increases later, and is observed as an increase in OUR and CER as well as an increase in acetate (ethanol is converted back to acetate and produces $ NADH per mol ethanol) It is interesting ... and benzoic acid will be in equilibrium inside and outside the cell following the intracellular and extracellular pH difference, as described in Eqn (4) Accordingly, intracellular pH can be calculated...
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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
Ngày tải lên :
07/03/2014, 16:20
... [29,30], we examined the ability of Ybr004cp-depleted cells to incorporate [3H]inositol into proteins The ybr004cD-pGALYBR004c strain was grown and labeled with [3H]inositol in medium containing galactose ... proteins in medium containing either galactose or glucose (Fig 1C, lanes 1, 2), whereas ybr004cDpGAL-YBR004c cells incorporated significantly less [3H]inositol into proteins in glucose-containing ... grown and [3H]inositol-labeled in glucose-containing medium Lipids were extracted from cells and incubated either with or without mild-base (lanes and 2) and with or without PI-PLC (lanes and 4)...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
Ngày tải lên :
08/03/2014, 23:20
... especially in the four hydrophobic domains (underlined sequences in Fig 1A), showed that domains I and III are most divergent in group and proteins The results we obtained indicate that these two domains ... cDNA and genomic cloning of sugar beet V-type H1-ATPase subunit A and c isoforms: evidence for coordinate expression during plant development and cooridinate induction in response to high salinity ... vector 167 and 168 amino-acid proteins [7,8] The primary structures of the six proteins are shown in Fig 1: they are divided into two groups, group (AACEVAPD1 and ) and group (AACEVAPD2, 4, and )...
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Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot
Ngày tải lên :
17/03/2014, 10:20
... charge, ATP and inosine were determined as described in Materials and methods H2O2 evokes a decrease and an increase in the intracellular concentration of ATP and inosine, respectively Searching for ... of adenine nucleotides, (b) the decrease in ATP and the increase in inosine, promoted by H2O2, cannot be explained solely by a change in the level of the enzymes more directly involved in the ... adenosine deaminase; E5, purine nucleoside phosphorylase; E6, adenylate kinase; E7, adenosine kinase; E8, hypothetical enzyme recycling ATP Fig Dierential equations describing the uxes operating in...
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Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx
Ngày tải lên :
22/03/2014, 21:21
... conditional strain in glucose medium (Figs 10A and S4), indicating that, although these proteins show sequence similarity in the C-terminal portion, divergences in the remaining sequence of the ... complex with Imp3p and Imp4p, and these proteins show interdependence for binding to U3 snoRNA [24] Because Utp25p showed no interaction with Imp3p and Imp4p and was not isolated in the Mpp10p ternary ... concentrated and plated in glucose containing medium in a 10-fold serial dilution For the growth curve in liquid medium, cells were grown in galactose containing medium until stationary phase and then...
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Báo cáo khoa học: Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch ppt
Ngày tải lên :
23/03/2014, 05:22
... of Sin3 and or Rpd3, rather than by regulating Sin3 ⁄ Rpd3 directly A good candidate for a factor regulating Sin3 ⁄ Rpd3 binding is Stb1, because it binds to both Swi6 and Sin3 [20,21,32,34] In ... corepressor Sin3 in a two-hybrid assay [34] Recent analysis of G1specific mRNA levels in stb1D and sin3D mutants suggested a role for Sin3 and Stb1 in regulating these genes [33] Sin3 and its associated ... Stb1 was originally identified as an interacting protein of Sin3 in two-hybrid assays (Stb1 for Sin three binding) [34] STB1 transcription was shown to be cell-cycle regulated and peaks in late G1...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
Ngày tải lên :
23/03/2014, 07:20
... following toxin-mediated damage in vivo Percentage depurination was determined by quantifying the intensity of the liberated fragment in relation to the remaining intact 25S rRNA plus fragment using ... profile to RTAD, again indicating some difficulty in assuming a tightly folded conformation By contrast, RTAE177D is mainly non-glycosylated with only a minor fraction carrying a single glycan This ... strains of yeast, in a manner akin to its expression in plant cells [18] Survivors of toxin expression may contain defects in genes normally essential for toxin retro-translocation, refolding,...
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Báo cáo khoa học: Protein glycation in Saccharomyces cerevisiae Argpyrimidine formation and methylglyoxal catabolism ppt
Ngày tải lên :
30/03/2014, 20:20
... argpyrimidine-modied proteins This indicates that glyoxalase II plays a minor role in maintaining a low intracellular methylglyoxal concentration in the presence of high GSH concentration (4 mm in Saccharomyces ... argpyrimidine-modied proteins, indicating that glyoxalase I and aldose reductase are equally important in preventing MGO-derived protein glycation in yeast In fact, the double mutant DGRE3GLO1 strain ... stained gel of the reference strain (BY4741) and mutant strains (DGRE3, DGSH1, DYAP1, DGLO2 and DGLO1) (B) Argpyrimidine formation in intracellular proteins from the same yeast strains as in...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
Ngày tải lên :
31/03/2014, 08:20
... b-galactosidase activity in wild-type and MRG19 disrupted strains bearing the indicated fusion constructs Transformants were grown to D600 of 0.5 and 1.5, in minimal synthetic medium containing glycerol ... MRG19 (lane and 3) and wild-type cells (lanes and 5) were allowed to grow to an OD600 of 0.5 and 1.5, either in uracil drop-out minimal medium containing glucose (lanes and 3) or in synthetic ... (Fig 10, lane and 2) and wild-type (Fig 10, lane and 4) strains bearing multiple copies of MRG19 and grown in the presence of glucose (Fig 10, lane and 3) and glycerol (Fig 10, lane and 4) It is...
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Báo cáo sinh học: "Motifs, themes and thematic maps of an integrated Saccharomyces cerevisiae interaction networ" pps
Ngày tải lên :
06/08/2014, 18:21
... containing protein-protein interactions or correlated expression links (Figure 1d) Protein-protein interaction is known to correlate positively with co-expression [1,2], and proteins corresponding ... protein complexes with many inter-complex SSL interactions For this purpose, we only considered links of protein-protein interaction and SSL interaction and reduced the original network to one in ... factors and chromatin-modifying machines Cell 1998, 92:307-313 Norden C, Liakopoulos D, Barral Y: Dissection of septin actin interactions using actin overexpression in Saccharomyces cerevisiae...
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Báo cáo sinh học: "Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae" doc
Ngày tải lên :
06/08/2014, 18:21
... protein-protein, protein-RNA and gene-gene interactions associated with all known or predicted proteins in S cerevisiae, analogous in structure to the BioGRID interaction database [43,53] Each interaction ... to proteins defined in the LC-PI dataset (7.3 interactions per protein in LC-PI) and a low-density region that corresponded to interactions between proteins not in the LC-PI dataset (2.8 interactions ... repeated over 100 random trials, in which the interactions of both networks are reassigned while maintaining the same number of interactions per protein, resulting in a distribution of random subnetwork...
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Báo cáo y học: "A compiled and systematic reference map of nucleosome positions across the Saccharomyces cerevisiae genome" ppsx
Ngày tải lên :
09/08/2014, 20:20
... thymidylate) in the promoter, which resist incorporation into nucleosomes, and in part by sequence-specific DNA binding proteins (for example, Reb1) that help position nucleosomes [17], and by the intrinsic ... nomenclature for identifying nucleosome positions, so as to maintain consistency in characterizing individual nucleosome function across datasets Currently, there is no standard in calling individual nucleosome ... mapping, the technology has been perfected What 'error' remains may largely be due to biological variation in positioning (phasing), which in many locations in the genome is nearly random, and...
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Báo cáo y học: " Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae" pdf
Ngày tải lên :
11/08/2014, 21:21
... protein in pDBLeu-E2 transformed yeast with anti-cMyc antibody as shown in Figure 1D Therefore, E2 protein could facilitate viral genome replication and maintenance Role of E2 protein in maintaining ... Microbiology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China 2Department of Pathology, Shandong University School of Medicine, Jinan, Shandong, 250012, PR China Received: ... maintain the mitotic stability of its genome in an E2-independent manner in yeast and mammalian cells [7,20,21] In contrast to HPV16 genome, the maintenance of HPV58 genome depends on E2 protein...
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