roseus cell suspension medium in response to uv b irradiation and its inhibition by suramin

báo cáo khoa học: " LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum" ppt

Ngày tải lên : 12/08/2014, 05:20

... protein and enzyme activities in memThe distributionobtained from pea seedlings The distribution of protein and enzyme activities in membrane fractions obtained from pea seedlings A microsomal membrane ... proposed signalling and regulatory roles For example, lysoPC and lysoPE have been proposed to act as second messengers to auxin [33] and to be involved in systemic responses in wounded plants ... GFP) to maintain a native ER membrane surface for the studies of membrane-membrane interactions The isolated plasma membrane fraction was subjected to cycles of freezing and thawing to invert...

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Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

Ngày tải lên : 19/02/2014, 00:20

... FEBS 2223 Membrane targeting of K-Ras G A Gomez and J L Daniotti (Beckman Coulter) Membrane binding of soluble K-Ras protein was assayed by incubation of S fractions obtained from transfected cells ... 80 at 300 mA Protein bands in nitrocellulose membranes were visualized by Ponceau staining For immunoblotting, nonspeciﬁc binding sites on the nitrocellulose membrane were blocked with 5% (w ... lysine residues by arginine or d-lysine residues in the polybasic domain of K-Ras does not interfere with plasma membrane localization of this protein [30], suggesting that binding of K-Ras to...

19
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx

Ngày tải lên : 08/03/2014, 22:20

... mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds ... H+-ATPase by fatty acids (Eur J Biochem 269) 1007 Ó FEBS 2002 Table Relevant biological characteristics of U maydis strains and treatments Genetic lesions and sterol biosynthetic steps inhibited by ... compounds [33] In U maydis, changes in the plasma membrane 18 : 2/18 : ratio followed the inhibition of growth rate, which was in uenced not only by the biosynthetic point affected but also by the method...

6
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Báo cáo khoa học: Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling pot

Ngày tải lên : 30/03/2014, 02:20

... complex to lipid rafts in neuronal cells and brain BMC Biochem 8, 18 Baird B, Sheets ED & Holowka D (1999) How does the plasma membrane participate in cellular signaling by receptors for immunoglobulin ... Prabhu U, Dubyak GR, Proctor RA & Bertics PJ (2001) Cutting edge: the nucleotide receptor P2X7 contains multiple protein- and lipid-interaction motifs including a potential binding site for bacterial ... (an antithrombotic drug) and its active compound derivative probably block the P2Y12 receptor by disassembling these oligomers and displacing them to non-raft domains [82] In contrast to these results,...

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Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

Ngày tải lên : 30/03/2014, 10:20

... thought to be dominated by the highly speciﬁc association to PIP2 via its ligand binding site, the afﬁnity-reducing effect of PS on the PH domain suggests that a mechanism independent of PIP2 binding ... site consisting of the b1 ⁄ b2 , b3 ⁄ b4 ⁄ and b6 ⁄ b7 loops The short amphipathic a-helix included in the b5 ⁄ b6 loop (a2-helix) appears to be responsible for the interaction with the interface ... the PH domain–IP3 complex (1MAI) [5] b- Sheets consisting of a b- sandwich core of the domain (b1 -b7 ) are indicated by rectangles and a-helices (a1-a3) are indicated by cylinders The alanine residues...

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Báo cáo hóa học: "Research Article Epileptic Seizure Prediction by a System of Particle Filter Associated with a Neural Network" doc

Ngày tải lên : 21/06/2014, 20:20

... parameters in (12) can be obtained Parameters α and β can be determined by minimizing errors, based on a known seizure A and B can be obtained by minimizing error wk One further step is to regression ... probability distributions of interest using a set of random samples called particles These particles are propagated over time following the corresponding distributions by sampling and resampling ... Transactions on Biomedical Engineering, vol 53, no 3, pp 524–532, 2006 [9] F Wendling, F Bartolomei, J J Bellanger, and P Chauvel, “Epileptic fast activity can be explained by a model of impaired GABAergic...

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Báo cáo lâm nghiệp: "-pumping + H activities of plasma membrane and tonoplast from Quercus rubra roots" pptx

Ngày tải lên : 09/08/2014, 04:20

... Quenching of ACMA (initial rate: 500%) was collapsed by nigericin and inhibited by N03 K value of pumping: 0.67 mM m mM) induced total inhibition This hydrolysis mediated a distinct proton translocation ... density membrane fraction is stimulated by K+, relatively insensitive to anions and inhibited by vanadate (Tables I and II) Based on these properties, the low density membrane ATPase may be of tonoplast ... an interior acidification of the membrane vesicles (inside-out vesicles); quenching was blocked by nigericin and the sensitivities of the H toward -transport + the inhibitors, vanadate (Fig ) and...

6
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báo cáo khoa học: "Prozone effect of serum IgE levels in a case of plasma cell leukemia" pot

Ngày tải lên : 10/08/2014, 22:21

... flow cytometry-, no evidence of graft-vs-host-disease, and MM in partial remission by serum M component and bone marrow biopsy Monitoring of disease response during the Page of treatment was based ... personnel The variability in serum IgE levels shown in Table may be explained by the challenge of diluting each sample a minimum of 10,000 fold, when standard medical laboratory techniques rarely ... IgE plasma cell leukemia successfully treated with combination VAD (vincristine, doxorubicin, dexamethasone) and MP (melphalan, prednisolone) followed by interferon-alpha Am J Hematol 1994, 45:262-264...

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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot

Ngày tải lên : 13/08/2014, 01:20

... signaling; (3) interleukin and interferon responses; and (4) cytoskeleton and cell adhesion In the cell cycle and apoptosis category, five pathways were directly involved in cell apoptosis including ... attributed to increased susceptibility to apoptosis from alterations in the cytokine milieu in lymphoid tissue, bystander effects from neighboring productively infected CD4+ T cells, and toxicity ... coordinately up-regulated in the LTNP group (versus VIR) in CD4+ and CD8+ T cells Activated AKT is known to promote cell survival by phosphorylating BAD and CASP9 to inhibit pore-forming in mitochondria...

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

Ngày tải lên : 19/02/2014, 07:20

... been observed also in response to cold or osmotic stress, and their binding to the H+-ATPase is suggested by the parallel increase of the number of FC-binding sites [18–20] As to auxin, a soluble ... et al ([1] and references therein) The auto-inhibitory action of the C-terminus is suppressed, besides by pH, by lysophospholipids and by 14-3-3 proteins ([1] and references therein) The latter ... activity, the C-terminus exerts its auto-inhibitory action, presumably by binding to an intramolecular site [1,23–27]; thus, it might hamper the access of PPI1588His6 FC-induced binding of 14-3-3 displaces...

8
629
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Báo cáo khoa học: Epoxidation of benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 in reconstituted membranes Effects of charge and nonbilayer phase propensity of the membrane pot

Ngày tải lên : 08/03/2014, 10:20

... Km and Vmax were changed by the same factor The most probable explanation is an increase in the effective substrate concentration which is probably due to the redistribution of the substrate between ... activation probably by increasing the effective substrate concentration in the membrane Thus, we found that in addition to the membrane charge, the nonbilayer phase propensity of the membrane is an ... N-terminal transmembrane domain have additional attachment region(s) associating the protein partially buried into the membrane [35] This would favour lipophilic substrates by placing the opening...

7
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx

Ngày tải lên : 23/03/2014, 07:20

... competitively inhibited by free CaM in the incubation medium (Fig 9A, +CaM) The bound PCaP1 was released and eluted by an SDS solution (Fig 9B) When free calcium was removed from the incubation medium by ... PCaP1 as the site of binding to PtdInsPs In general, the polybasic residue region is a good candidate for binding to PtdInsPs The N-terminal part is the most basic region, containing seven Lys residues ... pI value of 4.99 The polybasic region may bind to phosphate moieties on the inositol ring by electrostatic bonds Several PtdInsP2-binding proteins contain clusters of basic ⁄ aromatic residues...

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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21

... trypsin Generated tryptic peptides were separated by RP-HPLC, and subjected to N-terminal amino-acid sequencing (Fig 1B) To enable deduction of the complete amino-acid sequence of bovine MUC15 by ... amino-acid sequences of bovine and human MUC15 Fully conserved residues are indicated with black boxes Amino-acid sequence obtained by peptide mapping and Edman degradation of the bovine protein ... protein Removal of sialic acid by neuraminidase resulted in a slight decrease in the mobility of bovine MUC15 in SDS/PAGE (Fig 2) Presence of O-linked glycans was shown by incubating neuraminidase...

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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx

Ngày tải lên : 20/06/2014, 01:20

... predominant variants found in vaginal secretions, and assessed ART use in a cohort of 43 women attending an HIV outpatient clinic in New Orleans Informed consent was obtained in accordance with Louisiana ... confirmed by review of medical chart data Vaginal secretions were collected from the vaginal vault on Dacron swabs and subsequently eluted in phosphatebuffered solution Supernatant and cell fractions ... X4-utilizing strain, HIVHXB Following electrophoresis on a non-denaturing acrylamide gel, mobility ratios of the heteroduplexes formed between sample and probe V3 sequences were analyzed and used to...

4
481
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báo cáo khoa học: "Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells" potx

Ngày tải lên : 11/08/2014, 11:21

... reduction in elicited resistance by heat inactivation and by the presence of the cellulase inhibitor cellobiose Further, the effect of inhibitors excludes the possibility of alamethicin resistance being ... a faint signal could be observed in cells treated for 20 with CM medium, followed by 220 with Control medium (Figure 2) No staining was observed in the absence of alamethicin in any cells In the ... suggesting that the enzyme-induced activity on the cell wall was needed for the response Also, lowering the incubation time in the CM medium to an initial 20 followed by washing and incubation...

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báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc

Ngày tải lên : 12/08/2014, 05:20

... protein kinase capable of autophosphorylation in vitro [5] and moreover also be phosphorylated by Pto Tobacco plants over-expressing S1Pti show enhanced HR in leaves in response to avirulence factor ... phosphorylated by Pto and is involved in the hypersensitive response Cell 1995, 83:925-935 Zhou J, Tang X, Martin GB: The Pto kinase conferring resistance to tomato bacterial speck disease interacts ... after infection and of a mock infected cob (3 weeks uninfected) RNA was isolated from kernels of cobs and divided into top (T) middle (M) and bottom (B) Corresponding cobs are shown at the top...

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Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"

Ngày tải lên : 25/10/2012, 10:02

... caused by failure of beta-cells to overcome insulin resistance created by the anti-insulin hormones secreted by the placenta [48] The same risk factors predict GD and subsequent diabetes in women ... resistance Curr Opin Clin Nutr Metab Care 2002, 5:551-559 14 Marette A: Mediators of cytokine-induced insulin resistance in obesity and other inflammatory settings Curr Opin Clin Nutr Metab Care 2002, ... Consequent to inhibition of pancreatic beta-cells by cytokines and catecholamines, insulin concentrations may be normal or even decreased [17-19] Medical interventions, such as enteral and parenteral...

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656
1

Treatment of Textile Wastewater by a Coupling of Activated Sludge Process with Membrane Separation

Ngày tải lên : 05/09/2013, 09:08

... denimprocessing wastewater can be classified as rather easily biodegradable waste by aerobic microorganisms It is evident in Table that the increased SRT reduced the BOD/COD value thereby increasing ... subject to high operation cost due to recirculation An effort to minimize energy consumption has been done by several researchers Yamamoto [1989] incorporated hollow fiber membrane directly into ... velocity and pressure needed to maintain flux stability [Dijk and Roncken, 1997] In order to reduce flux decline, highly sufficient cross-flow velocity over the membrane surface has to be maintained...

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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

Ngày tải lên : 14/02/2014, 21:20

... distinguish total binding from non-speciﬁc binding, cells were incubated for 10 in either PBSCM alone or PBSCM containing 200 lm cocaine for inhibition of DAT or 200 lm S-citalopram for inhibition ... However, its insolubility in the neutral binding buffers made it impossible to study in the binding assay (data not shown) The full-length p25a protein was subsequently biotinylated to allow it to be ... The biotinylated proteins were captured by incubating with NeutraAvidin beads The total and surface-bound protein fractions were analyzed by reducing SDS ⁄ PAGE followed by Western blotting with...

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