roof plate pathway in the dorsal midline
Báo cáo khoa học: Probing the access of protons to the K pathway in the Paracoccus denitrificans cytochrome c oxidase pdf
... 4.8, the group being deprotonated above pH The band width of the contribution at 1718 cm)1 indicates that residues close to the surface, or in the vicinity of several water molecules are involved ... spectral contributions from the mediators in the visible and infrared range could be detected in control experiments with samples lacking the protein, except for the PO modes of the phosphate buffer ... redistributions at the cofactor sites, reflecting the reorganization of the hemes, of the polypeptide backbone and of the amino acid side chains upon electron transfer to ⁄ from the redox active...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... construct coding for a VRL-1-GFP fusion protein was prepared by cloning the open reading frame of rVRL-1 from rat brain RNA without the last codon for amino acid P761 and the stop codon into pcDNA3.1/CT-GFP-TOPO ... corresponded to the rat brain control, and the faster migrating band to the N18TG2 control (Fig 5) The existence of both rat and mouse VRL-1 in the same cell was confirmed by F-11 single cell RT-PCR In all ... distinguish between the two on the protein level using commercially available antibodies Due to the expression of the endogenous protein, the N18TG2 cells are appropriate for functional investigations...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" pptx
... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... minced in DMEM containing 10% FBS supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin The cell suspension containing small tissue pieces was plated in a 10 cm-culture dish and incubated ... OPCs to the neoplastic lesions In this study, we examined clinical GCT samples in order to determine the possible role of the VEGF-Flt-1-FAK pathway in the pathogenesis of bone destruction in GCTs...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" potx
... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... minced in DMEM containing 10% FBS supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin The cell suspension containing small tissue pieces was plated in a 10 cm-culture dish and incubated ... OPCs to the neoplastic lesions In this study, we examined clinical GCT samples in order to determine the possible role of the VEGF-Flt-1-FAK pathway in the pathogenesis of bone destruction in GCTs...
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Báo cáo y học: "Eotaxin and FGF enhance signaling through an Extracellular signal-related kinase (ERK)-dependent pathway in the pathogenesis of Eosinophilic Esophagitis" doc
... eosinophils also express bFGF [28] The eosinophils may be operating in a positive feedback loop in which the expression bFGF is encouraging the increased activation of the eosinophil, which then ... increase in FGF and ERK pathways was closely associated with the severity of the disease and endoscopic findings in the EoE patients; further patients will help determine if FGF and other immune indicators ... were involved in enrolling and consenting patients JH drafted the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing...
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Investigating the superoxide mediated survival pathway in the prostate cancer cell line LNCaP
... classified under the extrinsic and intrinsic pathway The extrinsic pathway involves the direct transduction of an external signal that activates apoptosis The intrinsic pathway is mediated by the mitochondria, ... causing the modification of the regulatory domains present on the C-terminal cytoplasmic domain The modifications result in the changes in affinity of the internal H+ transport site that determines ... anti-apoptotic Bcl-2 proteins, the pro-apoptotic BH123 proteins and the BH3-only proteins The α helices of the proteins are designated and the bold lines define the BH domains ‘TM’ marks the hydrophobic...
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Regulation of WNT beta catenin pathway in the disease progression of osteosarcoma
... β-catenin within the cytoplasm In addition to its role in gene regulation, β-catenin is involved in regulating cell-cell adhesion by acting as a structural adaptor protein linking E-cadherin to the ... tyrosine residue 654 of βcatenin by either c-scr or the epidermal growth factor receptor leads to the loss of Ecadherin binding while the tyrosine kinases Fer, Fyn or Met are capable of inducing ... proteins in the nucleus while another distinctive form that exists as a βcatenin-α-catenin dimer preferentially binds to E-cadherin at the plasma membrane [127] In the nucleus: Wnt/β-catenin-mediated...
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Anti inflammatory effects of inhibitors of the NF kb pathway in the mouse asthma model
... p52), which are the main DNA-binding units when they form dimers with other Rel proteins The Rel proteins including c-Rel, RelB, and RelA (p65), contain C-terminal transactivation domains which activate ... phosphodiesterase inhibition, but there might be other mechanisms for this drug The major limitation of theophylline is its narrow therapeutic index Therefore, theophylline is now considered as the second-line ... antiinflammatory effects by inhibiting the phosphorylation of IKKβ and suppressing the DNA-binding activity of p65 Taken together, these present findings implicate that appropriate and specific inhibition...
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Báo cáo y học: "Genetic polymorphisms in the nucleotide excision repair pathway and lung cancer risk: A meta-analysis"
... skin tumors at an extremely high frequency (1000 fold increased incidence as compared to normal individuals) because of their inability to repair UV-induced DNA lesions These clinical findings ... efficiency The nearby proximal nucleotides to the AUG initiation codon are important for the initiation of translation because the 40S ribosomal subunit binds initially at the 5'-end of the mRNA [61] The ... position -4 preceding the AUG codon may affect ribosomal binding and thus alter the efficiency of XPA protein synthesis To investigate whether the transition from G to A changes the translation...
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The ubiquitin-proteasome pathway in cell cycle control
... amino termini and one of several protein-protein interaction motifs carboxy ter- The ubiquitin-proteasome pathway in cell cycle control 157 minal to the F-box In addition, some members of the F-box ... different inhibitory complexes consisting or checkpoint proteins have been detected, it is not yet clear which ones are the end products of the spindle checkpoint pathways Another issue the remains ... by the checkpoint kinase Chk1, which inhibits ubiquitylation by the APC/C Another checkpoint kinase, Rad53/Chk2, also inhibits Pds1 ubiquitylation by an unknown mechanism Therefore in budding...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... compounds in the phloem exudates, amounting to 51% of the total amino acids in the light and 56% in the dark (Fig 7A) The total amount of alanine, serine and glycine was reduced from 33% in the light ... light to 25% in the dark (Fig 7A) Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino acids) and dark (60%) (Fig 7B) The amino acid percentage ... al acid synthesis in these cells, from which the amino acids are loaded to the phloem for the grain In the phloem sap, glutamine is the preferred nitrogen carrier rather than asparagine (Table...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
... tubes containing either heparin or buffer Monitoring of FVa activity in these two tubes was continued for another 25 min, during which time the loss of FVa activity in the absence of heparin (open ... of the heparin-binding site in these three crystallized protein–heparin complexes were given as starting point for the docking search [30] At least one conformation of the simulated protein–peptide ... obtained in the presence of heparin (closed circles) This indicates that the presence of heparin did not in uence the k306 in the partially active FVa intermediate In a second experiment, the...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... does not contain the gene encoding the deaminase reported here In the cloned 5.2-kb fragment from P pseudoalcaligenes JS45, there are no genes involved in the 2-aminophenol-metabolic pathway, except ... analysis, and the determination of released ammonia The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step The product ... 6-semialdehyde deaminase) in P fluorescens strain KU-7 [7] The decarboxylation mechanism in the metabolic pathways for 3-hydroxyanthralinic acid differs from that in the pathway for 4-amino3-hydroxybenzoic...
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Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx
... administered to establish the role of protein synthesis in insulin-induced HO-1 expression (Fig 2B,C) CHX abrogated HO-1 protein induction following insulin treatment but, in agreement with other ... identify signaling pathways involved in regulation of HO-1 gene expression This study presents data demonstrating PI3K ⁄ Akt-dependent induction of HO-1 following the administration of insulin to renal ... oxidative insult Insulin is a polypeptide hormone that regulates glucose, lipid and protein metabolism and promotes cell growth and differentiation On ligand binding, the insulin receptor tyrosine kinase...
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Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx
... rescue the RAS1 RAS2-double deletion, suggesting that SUT2 either acts upstream or, alternatively, in a parallel pathway to RAS To further investigate the relation between SUT2 and the RAS/cAMP pathway ... relieve this sterol precursor-starvation by increasing sterol-biosynthesis Therefore it will be interesting to determine if genes of the sterol biosynthetic pathway are subject to regulation by Sut2p, ... characterized as a homologue of the sterol uptake, biosynthesis and trafficking regulator SUT1 [11] In the present work, we investigate how SUT2 might be linked to the Ras/cAMP pathway High SUT2 gene dosage...
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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
... Performing partial least square (PLS) regressions For future metabolic engineering of strains with the purpose of obtaining increased NADPH levels and for the understanding of the regulation of the ... all the variables in this quadrant are positively correlated to NADPH in the two PCs The variables in the third quadrant are all negatively correlated to NADPH in the two PCs The variables in the ... FEBS The 6PG level was generally increased in the Gsd11 strain and generally decreased in the Gnd20 strain This is expected, as 6PG is the product and the substrate for the enzyme overproduced in...
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Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
... clarify the binding properties of each of these domains we generated synthetic mRNA encoding only the C1 or C2 domains Expression of only the C1 domain resulted in the dorsalization of embryos in ... FEBS BMP/activin pathway in Crassostrea gigas ted of its highly variable C-terminal domain after the terminal conserved arginine of the cytoplasmic serine ⁄ threonine kinase domain Sequences for ... of the TGFb superfamily type I receptors are in bold and underlined, and the cysteine knot is boxed Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine...
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Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... performed using LipofectAMINETM (Life Technologies Inc.) according to the manufacturer’s protocol 293T cells were maintained in minimum Eagle’s medium (MEM) containing 10% (v/v) fetal bovine serum ... or by the inhibitory Smad7 protein To assess the involvement of TrkA receptors in the activation of the Smad pathway, different TrkA variants were tested in reporter gene assays using the pSBE-luc ... mutually in uence their activity [27] TGF-b family members are implicated in multiple interdependent signals between pathways originating from receptor serine/threonine kinases and receptor tyrosine...
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Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx
... [26,27] Furthermore, by examining the interplay between SRP54, FtsY and the membrane, these studies provide insight into the events that take place during the SRP-mediated protein targeting in Archaea ... within the first 46 positions, while Pyrococcus furiosus FtsY contains 12 arginine and lysine residues in this portion of the protein In contrast, examination of the A domain sequence of FtsY in ... domains contain fewer arginine and lysine residues than A domains of other archaeal FtsY proteins The amino acid composition of the first 46 positions of archaeal FtsY proteins are shown Arginine...
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Báo cáo khoa học: Probing the interface between factor Xa and tissue factor in the quaternary complex tissue factor–factor VIIa–factor Xa–tissue factor pathway inhibitor pptx
... located in the TF1 domain, and T197 is situated in the N-terminal portion of the TF2 domain, i.e close to the TF1 domain at the level of EGF1 in FVIIa in the sTF–FVIIa complex (Fig 1) Binding of ... regarding the binding area between sTF and FXa in this complex Regarding the ternary sTF–FVIIa–FX complex other investigators have performed functional analyses to investigate the binding region ... plausible that the Gla domain of FXa is involved in binding to the C-terminal domain of sTF As we found that FXa is bound next to the Gla domain of FVIIa, it is highly likely that the Gla domains of...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25