role of the thymus and persistence of antigen in calcineurin induced tolerance of renal allografts in mgh miniature swine
Báo cáo khoa học: Oligomannose-coated liposomes efficiently induce human T-cell leukemia virus-1-specific cytotoxic T lymphocytes without adjuvant doc
Ngày tải lên :
14/03/2014, 23:20
... against tumors and infectious diseases This is the first study demonstrating the successful induction of specific CD8+ T cells against a human tumor antigen using OMLs in HLA Tgm in vivo and in PBMCs ... Uptake of the HTLV-1 antigenencapsulating OMLs by macrophages would have been an initial key event in the induction of the antigen- specific Th1 immune response Thus, the efficient induction of HTLV-1-specific ... prevent the relapse of ATL in postallogeneic stem cell transplantation patients The expression of Tax by the host cell targets them for attack by CTL, resulting in the elimination of the infected...
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Báo cáo sinh học: "Cytotoxic T lymphocyte responses against melanocytes and melanoma" pdf
Ngày tải lên :
18/06/2014, 22:20
... break tolerance in the development of vitiligo First, the stimulation and expansion of MAA-specific CD8+ T cells requires CD4+ T cell help in vivo during the “induction phase” Then, in the “effector ... employed qRT-PCR in examining whether the amount of MAA mRNA may correlate with the extent of CTL degranulation and cytotoxicity A minor difference was seen between the levels of MART-1 and gp100 mRNA ... in its design and coordination and drafted the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received:...
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báo cáo hóa học:" Cytotoxic T lymphocyte responses against melanocytes and melanoma" pptx
Ngày tải lên :
20/06/2014, 04:20
... break tolerance in the development of vitiligo First, the stimulation and expansion of MAA-specific CD8+ T cells requires CD4+ T cell help in vivo during the “induction phase” Then, in the “effector ... employed qRT-PCR in examining whether the amount of MAA mRNA may correlate with the extent of CTL degranulation and cytotoxicity A minor difference was seen between the levels of MART-1 and gp100 mRNA ... in its design and coordination and drafted the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests Received:...
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Báo cáo y học: "Impact of cytokines and T lymphocytes upon osteoclast differentiation and function" pot
Ngày tải lên :
09/08/2014, 10:20
... protecting against bone loss In contrast to the pro-resorptive roles of T lymphocytes, several other T cell-derived interleukins and cytokines have the capacity to inhibit osteoclast formation and ... of GM-CSF production is sufficient to override the inhibitory action of IL-18 In contrast, IL-12 predominantly increases the production of IFN-γ, which is the principal osteoclast inhibitor in ... differentiation, and its central role in regulating bone mass is exemplified by individuals with high bone mass phenotypes having activating mutations in the Wnt pathway Wnt signalling is also involved in...
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Báo cáo y học: "Control of mucosal virus infection by influenza nucleoprotein-specific CD8+ cytotoxic T lymphocytes" ppsx
Ngày tải lên :
12/08/2014, 15:20
... proteins [15] or the M2 protein [17] The objective of the present study was to define the role of CD8+ CTL directed toward the NP protein in defense against influenza virus infection of the respiratory ... prevention of influenza infection requires induction of antibody to the surface viral hemagglutinin (HA) or the NA in both serum and respiratory secretions [43] An obvious implication of the present findings ... vaccination, mice were anesthetized by subcutaneous injection of 25 μl of ketamine-xylazineacepromazine cocktail (37.5/1.9/0.37 mg/ml) The legs of the mice were then flooded with 70% ethanol and...
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Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference pptx
Ngày tải lên :
18/02/2014, 13:20
... the Ile17 in almost all of the ERF family (V17 in CBF1), V27 in ERF subfamily (Ile27 or Leu27 in the DRE-binding protein subfamily) and Tyr42 in the major ERF family (His42 in the CBF1 and TINY ... Materials and methods Preparation of ERF domain-containing proteins The coding region of the ERF domain of CBF1 (UniProtKB: P93835) (amino acids 47–142), which contains 10 and 38 amino acids in the ... existed in the other positions within the binding motifs, discriminating the members from different subgroups The solution structure of the complex formed by the ERF domain of AtERF1 with the GCC...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the buffer Therefore, a fixed aliquot of the protein was incubated with the indicated ... basic proteins it might be that either methylation of the lysine or specific substitutions in the amino acid sequence, increasing the number of basic residues of lysine, arginine and histidine, could ... protein belonging to the GTPbinding elongation factor family, which promotes the GTPdependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of deacylated...
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Báo cáo khoa học: Tec family kinases Itk and Rlk⁄ Txk in T lymphocytes: cross-regulation of cytokine production and T-cell fates pot
Ngày tải lên :
28/03/2014, 22:21
... regulatory cells can either develop in the thymus or differentiate in the periphery The central role of cytokines in driving the differentiation of these subsets has been an active area of research [10] ... signaling pathways play critical roles in helping shape immune responses by in uencing the differentiation and homeostasis of cytokine-producing T cells Whether common themes are involved in these ... family kinases in Th1 and Th2 differentiation A number of studies have addressed the role played by Itk and Rlk ⁄ Txk in the regulation of cytokine-producing populations in vivo during pathogen infections...
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báo cáo hóa học:" Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients" pot
Ngày tải lên :
18/06/2014, 15:20
... proteins in the tumor tissues As shown in Table 1, survivin expression was detected in all the cases by immunostaining Though there were some differences in the staining intensity among the cases, ... 1B) By using more sensitive RT-PCR analysis, expression of these splicing variants was shown only in the thymus [22] We then analyzed the survivin expression in the protein level In all the oral ... carried out the CTL induction, killing assays and drafted the manuscript TT and YH participated in the design of the study and performed the evaluation of the data TT helped to draft the manuscript...
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Báo cáo y học: " Multiple functions for CD28 and cytotoxic T lymphocyte antigen-4 during different phases of T cell responses: implications for arthritis and autoimmune disease" pot
Ngày tải lên :
09/08/2014, 01:23
... transition leads to the skipping of exon 2, resulting in an increase in the expression of a ligandindependent isoform of CTLA-4 [89] In humans, a new CTLA-4 polymorphism was found in the 3′ untranslated ... affects the splicing of CTLA-4 mRNA; interestingly, this results in a lower expression of the soluble form of CTLA-4 mRNA The authors of the study speculate that a reduced interaction of B7 and soluble ... IL-2 and interferon-γ [21], as well as the expression of G1-kinases, a prerequisite for cell cycling In addition, the induction of IL-2 leads to autocrine support for the activation of the cell...
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Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx
Ngày tải lên :
12/08/2014, 23:23
... study integrase-deficient virus, taking advantage of the capacity of unintegrated DNA to express Tat and Rev and thereby induce GFP expression [20] Introduction of the D116N mutation into the integrase ... to introduce termination codons in the first and third amino acids of the env gene (construct termed pNL4-3-ΔE) Further modifications by mutagenesis included the substitution D116N in the integrase ... coding sequence of the pol gene (construct termed pNL4-3-ΔE-D116N) and the introduction of termination codons into the first and third codons of the nef gene (constructs termed pNL4-3-ΔE-ΔN and...
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Báo cáo y học: "Effects of naturally-arising HIV Nef mutations on cytotoxic T lymphocyte recognition and Nef’s functionality in primary macrophage" pptx
Ngày tải lên :
13/08/2014, 01:21
... Although the amino-acid sequences in the epitope region of A24-Nef were the same among the wt and these variant viruses tested, the CTL-mediated killing activity toward MDMs infected with M20A and ... replication of the 85F variant virus was partially impaired in MDMs from one of the donors and was comparable to that of the wt virus in MDMs from the other donor (Figure 6A) In contrast, the replication ... 6B Because the peak of the virus replication was between to 12 days after infection, depending on the donor and the virus, the peak p24 Ag values of each of the viruses are presented and were used...
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Báo cáo y học: " A balanced transcription between telomerase and the telomeric DNA-binding proteins TRF1, TRF2 and Pot1 in resting, activated, HTLV-1-transformed and Tax-expressing human T lymphocytes" pot
Ngày tải lên :
13/08/2014, 09:21
... transducer of the cis-inhibitory effect of the TTAGGG-repeat-binding protein TRF1 [6] The shelterin subunit TRF2 [7,8] is also involved in a negative regulation of telomere lengthening but by ... at the level of telomere itself, by several shelterin subunits, including TRF1, TIN2, TPP1, Pot1 and Rap1 For instance, Pot1, a single-stranded telomeric DNA-binding protein, behaves as a terminal ... function of numerous genes involved in the proliferation of T cells, is implicated in the initiation of the leukemogenic process [35-39] In spite of this low level of telomerase activity, HTLV-1 in...
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Báo cáo y học: " A peptide-loaded dendritic cell based cytotoxic T-lymphocyte (CTL) vaccination strategy using peptides that span SIV Tat, Rev, and Env overlapping reading frames" ppt
Ngày tải lên :
13/08/2014, 09:21
... &7) We interpret the emergence of these amino acid changes in the face of a lack of in vivo control of challenge virus to mean that CTL-responses in a priori coding-frame dense portions of the SIV ... plasmid Individual clones were then isolated and sequenced In parentheses, the numerator indicates the number of clones with a given sequence and the denominator shows the total number of clones ... emerge by correcting the stop codons which prevent the expression of the second coding exon of Tat Our interpretation of this scenario in the context of our operational definition of an "immutable...
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Vietnamese Speech Recognition and Synthesis in Embedded System Using T-Engine
Ngày tải lên :
12/04/2013, 16:05
... synthesis into our software running in T-Engine This software allow users to use speech-commands to query information about places in Ha Noi Figure illustrate the main screen of the software, in ... engine, we separate the training phase from the recognizing phase in order to reduce the system resource used in T-Engine The training phase is implement in a PC with speech data from T-Engine, ... end-point of the first frame, is also the start-point of a period of the vowel This field is available for the first diphone only End-point of n period End-point of n+1 period End-point of n+1 period...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
Ngày tải lên :
06/03/2014, 22:21
... end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity of the ... Levels of TfR2 are highest in hepatocytes, the predominant site of HFE expression, and recent studies have confirmed that the two proteins are capable of interacting [33,34] The nature of these interactions ... with b2 microglobulin (b2M) [4,5,8–11] In the absence of b2M binding, the protein misfolds and is retained in the endosplasmic reticulum (ER) where it induces an unfolded protein stress response...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf
Ngày tải lên :
16/03/2014, 18:20
... recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and Cb2 amino acid sequence and the localization of the domains ⁄ antigens ... that could be inhibited by protein kinase inhibitor (PKI) In the presence of cAMP approximately one-third of the PKI-inhibited activity was released into the extract, indicating that the precipitated ... procedure the R subunits associated with the antibody-immobilized Cb2 isoform would remain in the pellet in the absence of 8-CPT-cAMP while they would be released to the supernatant in the presence of...
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Báo cáo khoa học: Novel aspects of calmodulin target recognition and activation pptx
Ngày tải lên :
17/03/2014, 09:20
... molecule The portion of the K+-channel fragment containing the two helices is bound to the C-terminal lobe of CaM The EF-hand pair of the C-terminal lobe remains Ca2+ free and the binding of this ... portion of the K+-channel provides interesting insights into the mechanism of Ca2+ activated gating of the channel and presents a new way of CaM binding domain recognition The structure of the complex ... complementarity of binding surfaces determine the binding orientation of the peptide in complex with CaM The second interesting feature is the conformation of the CaMKK binding domain peptide in complex...
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Báo cáo Y học: Interferon-alpha inhibits Stat5 DNA-binding in IL-2 stimulated primary T-lymphocytes doc
Ngày tải lên :
31/03/2014, 15:20
... bandshifts during the second wave of IL-2 induced binding at 24±48 h (Fig 3A) Furthermore, IFN-a cotreatment also caused the appearance of a novel bandshift in- between the two IL-2 induced bands ... DNA binding activity in the cells (Figs 3,4 and 5) The links between IL-2 signalling and activation of the cell cycle in T-cells are poorly understood Activation of the PtdIns3K pathway in the ... ®nding that IFN-a inhibited the IL-2 induced expression of Jak3 protein levels and prolonged Stat5 DNA-binding and transcription in T-cells may be of importance in contributing to the antiproliferative...
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