... show the critical roleofthe last amino acids in the C-terminus of MaL Deletion ofthe four last amino acids strongly reduced the expression ofthe protein in T reesei and S cerevisiae Furthermore, ... change in the C-terminus of MaL (L559A) changed the catalytic propertiesofthe laccase The solved three-dimensional structureofthe protein with the L559A mutation revealed conformational changes ... and determined the inhibition constants for sodium azide, in order to see the effect ofthe mutation (L559A) onthe binding properties in the trinuclear center The inhibition of MaL by azide...
... region ofthe C-tail ofthe D1 receptor might be involved in the interactions between the two dopamine receptors In addition, the subcellular localization of D1–CFP, D2–YFP and all the mutants of ... and their interactions with the appropriate a subunits of G protein, further confirm that the use of advanced fluorescence techniques does indeed allow for the observation of true interactions The ... residues in the C-tail ofthe D1 receptor The efficiency of energy transfer was reduced to 0.8% A possible interpretation ofthe data suggests that the indicated basic region of ic3 ofthe D2 receptor...
... isolation The “what, whys, and hows” related to changing theroleofthe auditor has to be considered within the context ofthe overall financial reporting system, including the important roles ofthe ... to convene investors and other financial reporting stakeholders to examine theroleofthe auditor and the value ofthe audit On June 21 the PCAOB issued a concept release on potential revisions ... recommendations This report concludes with identification ofthe CAQ’s short and longer-term next steps to further pursue observations regarding theroleofthe auditor Appendix A to this report contains...
... allows the interaction ofthe enzyme with the substrate structure Whether the motion ofthe lid promotes the recognition ofthe substrate structure, or the recognition of this structure promotes the ... presence ofthe Cc domain and bile salt only in the presence ofthe C2 domain The inhibition ofthe active site serine by E600 needs both the motion ofthe flap and the recognition ofthe vehicle ... important information concerning thestructure function relationship ofthe PL [24–28] In agreement with biochemical data, these structures demonstrate the functional organization ofthe PL into two...
... contrasting reports about the interaction ofthe 4Â-PP group with the polypeptide backbone and its effect onthe stability of holo-ACP The average major conformation ofthe holo-ACP NMR structure was analyzed ... elucidates the interactions responsible for the conformational stability of PfACP The size exclusion chromatography prole of PfACP showed that the apparent molecular mass of PfACP monomer is 25 ... between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface ofthe protein because ofthe interactions ofthe cofactor with the protein...
... maintaining the conformation ofthe whole PBD and modulating the interdomain interface These findings, together with comparison ofthe thermal stability, trypsin accessibility and stimulation by co-chaperones, ... exchange at the PBD of DnaK did not modify the overall secondary structureofthe chimeras The intrinsic fluorescence of DnaK has been widely used to follow allosteric conformational changes upon nucleotide ... nucleotide the emission maximum of mtHsp70 is downshifted by 5–6 nm with respect to that of DnaK The twofold reduction ofthe KSV values estimated for mtHsp70 both in the absence and the presence of...
... proposition of a text The DT best represents the redundancy structureofthe text As such, this proposition functions as a reference point for processing the rest ofthe propositions The text position ... appear at the end ofthe review (instead ofthe beginning ofthe review) as a means of recapitulating the point ofthe message, thereby guaranteeing that it is fully understood by the readership ... than the first one (contrary to topic-extraction) Based on preliminary investigations, we suggest that the DT of any customer review is the customer’s evaluation, whether negative or positive, of...
... localization ofthe fusion protein correlates not only with activation of FRM2 transcription, but also with growth in increasing concentrations of cadmium but not of H2O2 Because ofthe high degree of ... the formation of an intermolecular bond occurs between the Orp1 Cys36 and the Cys598 of Yap1 leading to its activation The subsequent formation ofthe Yap1 Cys303-Cys598 disulfide bond masks the ... Induction ofthe Msn2 ⁄ target genes in response to one form of stress gives rise to the phenomenon of cross-protection against an aggravated form ofthe same stress or to a different type of environmental...
... groups The SEM micrographs also confirm the presence ofthe attached groups onthe outer walls of MWCNTs The presence ofthe different chemical groups (-COOH, -SO H, and -PO3H2) onthe walls ofthe ... studies The extent of functionalization with different groups has been determined from the EDX results and chemical routes which results in the identification of sulfonation and phosphonation of higher ... and double-step chemical routes The effect of dispersion using Triton X-100 via ultrasonication, before the functionalization of CNTs, has also been studied The defect concentration has been determined...
... contribution The present investigation was also limited by the fact that the standing rearfoot angle was the only structural measurement ofthe foot For instance, the range of motion (ROM) of ... part ofthe stance phase There appeared to be no relationship between the static anatomical structureofthe rearfoot with the magnitude ofthe change in rearfoot kinematics following fatigue Contrary ... in a bid to further understand theroleof this specific muscle on foot kinematics The main finding of this investigation was the significant increase in rearfoot eversion excursion during postfatigue...
... Chapter 24, The biosynthesis of Amino Acids) Glutathione conjugation is one ofthe systems in the detoxification of many anticancer drugs The major components of this system include glutathione (GSH), ... mediate the transport ofthe glutathione conjugates LTC4 and DNP-SG, the endothelin receptor antagonist BQ123, but not the glucuronate conjugate E217ßG or cyclic nucleotides The ability of MRP6 ... which suggest that they might involved in the regulation ofthe intracellular concentration of these important second messengers The cyclic nucleotides, cGMP and cAMP are second messengers involved...
... along the gradient of environmental conditions from the northern to the southern Red Sea? How the soft coral communities respond under differing conditions of impact from different pollution ... adaptation ofthe holobiont towards environmental conditions Barneah et al (2004) reported that the vertical transmission belongs to Symbiodinium clade A while horizontal transmission belongs to the ... estimation ofthe average annual environmental condition The study sites of Sawall et al (2014) were the same as in this study Additionally the silicate concentration was determined from the same...
... [26] These interactions have been reported to induce the disruption of cell–cell junctions, a loss of cell polarity and the induction of pro-inflammatory and mitogenic responses (Fig 3C) The first ... in the amino-terminal region of CagA [28] In particular, CagA induces the ubiquitination and degradation of RUNX3, thereby extinguishing its ability to inhibit the transcriptional activation of ... alteration of host-cell transcription signalling pathways Another important consequence of phosphorylationindependent CagA interactions in polarized epithelial cells is the disruption of cell–cell...
... focus on measuring the functioning ofthe financial system rather than on examining the direct impact of financial sector policy, regulations, and the rest ofthe enabling environment on economic ... Brothers marked the onset ofthe largest global economic meltdown since the Great Depression The aftershocks have severely affected the livelihoods of millions of people around the world The ... external contributions Note that The World Bank does not necessarily own each component ofthe content included in the work The World Bank therefore does not warrant that the use ofthe content contained...
... two activities, the hydroxylation of monophenols and the oxidation of o-diphenols Different names are used for these activities, as shown in the Figure One ofthe most common monophenolic substrates ... to be part ofthe defense system against wounding and infection by the pathogen [16] Thus, the timing and location of melanization seems to be essential for the prevalence of one of these two ... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4] Looking at the sequences ofthe two enzymes, both show absolute conservation ofthe histidine residues ofthe CuA...
... complex at the position ofthe histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex III revealed the presence of these complexes ... and the effect ofthe absence of intact complex I, but the results are too preliminary in this regard Heterologous expression ofthe ESSS-HA subunit The localization ofthe ESSS subunit in the ... with the incorporation ofthe tagged ESSS subunit into a functional complex I (Fig 3B, right panel) The signal from the 18 kDa subunit of complex I (NDUFB6) served as another identification of the...
... The solution structureofthe SEA domain ofthe mouse homologue of MUC16 (which in the human encodes the core protein ofthe ovarian cancer antigen CA125) was recently solved by multidimensional ... side ofthe loop, faces solvent and its mutation to alanine has little if any effect onthe cleavage event Onthe other side ofthe loop, the valine residue (V189) that interacts only with the ... cleavage than are the 33 amino acid substitutions Another feature ofthe hybrid mucins is that that they lack motifs ofthe native MUC3 and MUC12 that may contribute to the generation ofthe cleavage...
... information about the mode of action and the availability of a refined three-dimensional model [16] prompted us to prepare a series of synthetic fragments designed onthe basis ofthe secondary structure ... results obtained by other groups who also point to theroleofthe connecting loop ofthe b-hairpin of defensins A combination of mutational analysis [35] and structural analysis ofthe plant defensin ... plotted against the log ofthe measured MIC The aforementioned results showed that synthetic peptides corresponding to fragments ofthe MGD1 defensin reproduced the behaviour ofthe entire molecule...