role of oxidative stress and antioxidants in male infertility
Báo cáo khoa học: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development doc
Ngày tải lên :
23/03/2014, 07:20
... PARP inhibitor, on oxidative stress- induced changes in
D. discoideum development. Interestingly, oxidative stress resulted in PARP
activation within 5 min that was inhibited by benzamide. Oxidative ... development.
HA-induced oxidative stress activates PARP within
5 min (Fig. 4A,B), and its role during oxidative stress
is further confirmed by the use of low concentrations
of benzamide. Preincubation of cells ... the response of this organism to oxidative stress
and the role of PARP during development. We used hydroxylamine (HA)
to induce in situ generation of H
2
O
2
and monitored the effect of
benzamide,...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
Ngày tải lên :
23/03/2014, 15:21
... Nonretained proteins were washed out with 20 m
M
triethanolamine buffer pH 8.0 and retained proteins were
eluted using a gradient of NaCl prepared in the same initial
buffer. The presence of the ... clearly increased. Actually, the substitution of R97 by
methionine or lysine resulted in the same increment in the
pK
a
of E399 (Table 2). Therefore, M97 and K97 are equally
effective in stabilizing ... to
E187, in order to minimize unfavourable interactions with
the apolar side chain of F331. Thus, the increment in the
pK
a
of E399, in addition to it being closer to E187, may
have resulted in a...
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Báo cáo hóa học: " An exploration of job stress and health in the Norwegian police service: a cross sectional study" docx
Ngày tải lên :
20/06/2014, 00:20
... acquisition,
analysis and interpretation of data and drafting of the
manuscript. EH was involved in design, interpretation of
data, drafting of the manuscript and supervision. ØE was
involved in conception and ... Brown J, Fielding J, Grover J: Distinguishing traumatic, vicarious
and routine operational stressor exposure and attendant
adverse consequences in a sample of police officers. Work
Stress 1999, ... was
involved in conception and design, interpretation of data,
drafting of the manuscript and supervision. BL was
involved in analysis and interpretation of data. AMB is the
guarantor for this...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... significant insights into the roles and mechan-
isms of PM
2.5
-associated physiology and pathology.
In summary, our data demonstrate the important
effects of PM
2.5
exposure on oxidative stress and ... effect
of ambient PM
2.5
onWATandBATspecificgenesin
different adipose depots. There are several major find-
ings in this study. First, exposure to PM
2.5
resulted in
oxidative stress in BAT. ... blot.A.RepresentativebandsofFAandPM
2.5
on
UCP1 protein level in iBAT by Western blotting. B. Quantitative results of Western blotting of UCP1. n = 8. *P<0.05 vs. FA.
Xu et al. Particle and Fibre Toxicology...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
Ngày tải lên :
16/03/2014, 22:20
... that the mitochondrial injury is caused by open-
ing of the mPTP. There is evidence that ADP inhibits
opening of the mPTP by occupying binding sites
located in the inner and outer mitochondrial ... reperfusion in
brain.
There is a growing body of information concerning
the mechanism of ROS generation by the respiratory
chain in mitochondria. Complex I and complex III
were identified as generators of ... and oxygen consumption
was analysed. The corresponding values are shown in
Fig. 1. After 15 min of incubation in the presence of
3.5 lm Ca
2+
in an air atmosphere, state 3 respiration
of brain...
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Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf
Ngày tải lên :
22/03/2014, 16:20
... A rapid and sensitive method for
the quantitation of microgram quantities of protein
utilizing the principle of protein-dye binding. Anal
Biochem 72, 248–254.
CYP2E1 causes apoptosis of cardiomyocytes ... College, Beijing, China
2 Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Institute of Laboratory Animal
Science, Chinese Academy of Medical ... overexpression of CYP2E1 both in vivo
and in vitro is associated with several cellular markers
of oxidative stress, including products of lipid peroxi-
dation (e.g. 4-hydroxynonenal and malondialdehyde),
as...
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Báo cáo khoa học: The silencing of adenine nucleotide translocase isoform 1 induces oxidative stress and programmed cell death in ADF human glioblastoma cells doc
Ngày tải lên :
29/03/2014, 09:20
... [41], and human U87-MG
cell line, a kind gift of Professor C. Martini (Department of
Psychiatry, Neurobiology, Pharmacology and Biotechnol-
ogy, University of Pisa, Italy), were maintained under ... by trypsin treatment
and stained for 30 min at 37 C in fresh medium containing
JC1 (nal concentration of 10 lgặmL
)1
). Cells were then
washed twice with PBS, suspended in 500 lL of PBS and
analyzed ... ultrastructural evidence of membrane rup-
ture and were also negative to propidium iodide stain-
ing (data not shown), indicating that the plasma
membrane is maintained intact, allowing us to exclude
primary...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
Ngày tải lên :
19/02/2014, 16:20
... on
conformation of inhibitor main chain. Thus, different
inhibitors differ not only in t he binding affinity, bu t a lso
in the degree o f freedom of the inhibitor inside the binding
tunnel. Some s pecial inhibitors ... found
rotatedtoeachother.TheanglesbetweenplainsofP
3
Â
phenyl rings are 98 for OE and SE inhibitors, and 38 for
OE and RE inhibitors. The S3Â groups of OE and RE
inhibitors having a similar orientation phenyl rings form a
similar ... of projections of opposite S
d
atoms of M et46 and Met146).
Ó FEBS 2004 HIV-1 Protease Inhibitors (Eur. J. Biochem. 271) 4457
Inhibitor binding. The inhibitor OE b inds into the p rotease
binding...
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Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx
Ngày tải lên :
21/02/2014, 03:20
... determine the
effects of Hsp105a on JNK and p38 signalling pathways, we
examined the activation of JNK and p38 in F9 cells by
oxidative stress. As shown in Fig. 6A, JNK was not
activated in control ... activation of p38 induced by
oxidative stress, we further examined whether its activation
is responsible for the induction of apoptosis in F9 cells using
SB202190, a potent inhibitor of p38. As shown in ... peroxide. In contrast, hydrogen peroxide-treat-
ment induced marked activation of JNK within 30 min in
S23 cells, but not in S3 cells. Therefore, the enhancement of
the oxidative stress- induced...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... the binding of
GTP to the allosteric site promotes coordination of the
phosphorylation of UTP and hydrolysis of glutamine for
optimal efficiency in CTP synthesis rather than just acting to
increase ... kinetics of the glutaminase reaction of CTP synthase
from
Lactococcus lactis
The role of the allosteric activator GTP in coupling between glutamine hydrolysis
and CTP synthesis
Martin Willemoeăs
1
and ... role
for GTP activation of CTP synthesis. A mechanism in
which GTP only acts to increase the rate of hydrolysis of
glutamine has some flaws in explaining the inhibition of
DON-labeledenzymewhenassayedwithNH
4
Cl...
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