... Differential effects of cigarette smoke on oxidativestressand proinflammatory cytokine release in primary human airway epithelial cells andin a variety of transformed alveolar epithelial cells ... brightly stained with ethidium bromide and appeared red Percentage of viable (white bars), apoptotic (grey bars) and necrotic/late apoptotic (black bars) determined by counting as described in Materials ... evidence of apoptosis in human lung cancer cells (H1299) Figure Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in human lung cancer cells (H1299) Human lung cancer...
... mediators It is also involved in the progression of tissue injury during inflammatory conditions, implicating the roleof airway/airspace epithelium in the pathogenesis of inflammatory airway ... responsible for CSE induced pro-inflammatory cytokine release Our findings in SAEC gain credence from previous studies in human subjects wherein an increased expression of NF- Page 18 of 20 (page number ... transferrin, μg/ml insulin, 0.1 ng/ml retinoic acid (RA), 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B (GA-1000), and 50 μg/ml fatty acid-free bovine serum albumin (BSA) Polymyxin...
... wild plants have enormous economic and cultural importance and potential, providing nutrition and medicine to populations throughout world Many species of plants, including those ofmedicinal ... retain control over the future of the resource 15.2.2 Organizations Involved in Conservation ofMedicinalPlantsand Their Ecosystems The important topic of ethnobotany and the sustainable use of ... objectives, including (1) halting global trade in endangered animals and plants; (2) creating and preserving parks and protected areas around the world; (3) working to create strongholds for thousands of...
... resurgence in the incidence of TB occurred in the United States and included increases in the number of TB cases among adults and children and outbreaks of multidrug-resistant TB (MDR-TB) involving ... of skin testing is the Mantoux tuberculin skin test using 0.1 mL of tuberculin units (TU) of purified protein derivative (PPD) (1 ) BCG vaccination contributes to the prevention and control of ... preventing pulmonary TB in adolescents and adults These studies also were not useful in determining a) the efficacy of BCG vaccine in HCWs or b) the effects on efficacy of the vaccine strain administered...
... of the a-aminoadipoyl- side-chain of penicillin N (1), with arginines 74 and 75 apparently playing a less important rolein side-chain binding Taken together, the results of this and previous ... consistent with loss of penicillin binding Arginines 306 and 307 Fig Proposed binding mode for penicillin N to the iron(II)-2-oxoglutarate complex of DAOCS, showing possible involvement of selected residues ... productive penicillin (prime) substrate The results in this paper give support to the proposed mode of penicillin binding by DAOCS [1,12] a key feature of which is that arginines 160 and 162 bind to the...
... Vine Shrub Shrub Herb Vine Vine Tree Vine Herb Vine Vine Vine Vine Vine Herb Tree Vine Herb Epiphyte Epiphyte Vine Vine Vine Vine Tree Herb Herb Tree Vine Vine Vine Vine Herb >100 2±6 2±6 21±50 ... at least wind-protection and semi-shade), appropriate training, cultural acceptance, and ultimately, raising awareness of the need for sustainable harvesting ofmedicinal plant species in the wild ... addressing harvesting sustainability of key or target species Principles and guidelines concerning MP conservation database design, networking and maintenance, are outlined in Synge and Heywood (1991)...
... became increasingly criticized by international activists as a source of poverty and environmental degradation in developing countries One of the most important turning point was the WTO Ministerial ... combining two types of sustainable management of natural resource in a single economic equation and also under a single diagram as represented by: C ≦ ΔOS*E + S therefore, E ≧ (C – S)/ΔOS1 (b) Introduction ... Original Sustainability, S for Sustainable Level for human activities ΔOS means the marginal increase of consumption against environmental amenity while original sustainability is maintained The degradation...
... experimentation and innovation [17] This may explain the use of many herbs in the traditional medicinal practice in Babungo Leaves ofplants have been reported to accumulate, inulins, tannins and other ... Complementary and Alternative Medicine 2005, 5:6 25 Cunningham AB, Mbenkum FT: Sustainability of harvesting of Prunus Africana bark: A Medicinal Plant in International Trade People andPlants working paper ... http://www.ethnobiomed.com/content/6/1/8 Page of Table 3: Non medicinal uses ofmedicinalplantsin Babungo (Continued) Ocimum gratissimum L Lamiaceae Fulungfu The plant serves as insect repellent Piliostigma thonningii (Schum.)...
... Species ofMedicinalPlantsin South Asia Medicinaland Aromatic Plants Program in Asia (MAPPA), IDRC/SARO, New Delhi, India 1999 Ghimire SK: Sustainable Harvesting and Management ofMedicinalPlants ... effective monitoring of the local community resources will be the fundamental ingredient ofin situ conservation ofmedicinalplantsin Mustang Over-harvested populations ofmedicinalplants from ... practitioners maintain a great depth of knowledge on the subject ofmedicinalplantsMedicinalplants still play a pivotal rolein the primary healthcare of the local people in the study area...
... of C22 or C28 affect either cell surface binding or appropriate trafficking of virions during infection Campos and Ozbun observed indistinguishable cell surface binding and uptake into Page of ... http://www.virologyj.com/content/6/1/176 Figure Impact of L2 C22A and C28A point mutations upon vaginal infection of mice by HPV16 pseudovirions Impact of L2 C22A and C28A point mutations upon vaginal infection of mice by HPV16 pseudovirions ... epitope [1,4] This process of revealing the 17-36 epitope Figure Binding of RG-1 antibody to disulphide hairpin of HPV16 L2 Binding of RG-1 antibody to disulphide hairpin of HPV16 L2 An HPV16 L2...
... mechanism involving COX2 and PGE2 expression in the resolution of inflammation Page of 11 Proinflammatory cytokines, including IL-17 and TNF-a, play a critical rolein the progression of synovitis and ... cells in the presence of indomethacin Rheumatoid ST contains a number of proinflammatory cytokines that influence osteoclast formation and bone resorption Proinflammatory cytokines such as TNF-a and ... Effect of interleukin (IL)-17 on pannus-like tissue growth in the presence of indomethacin Synovial tissue (ST)-derived inflammatory cells (n = 7) were incubated with an incremental dose of IL-17 in...
... repression of genes involved in central carbon metabolism and purine/pyrimidine biosynthesis in B subtilis was said to occur independently of relA, and, therefore, presumably also of ppGpp synthesis, in ... Conway and co-workers have also reported a central role for ppGpp in coordinating the global down-regulation of sets of genes involved in active growth in E coli during glucose-lactose diauxie, and ... operates in streptomycetes [48,49] Our results indicate that ppGpp may have a role to play in these processes in S coelicolor, promoting the synthesis of non-zinc-dependent ribosomes and increasing intracellular...
... economic resource This thesis introduces a new concept of combining two types of sustainable management of natural resources in a single economic equation and also under a single diagram It has been ... degradation of the global environmental assets Many efforts are being made by individuals, governments, NGOs and international organizations including Green Peace and WWF International organizations and ... purpose of this thesis is to examine how International Agreements envisage to contributing to conservation of the global environmental assets that are diminishing rapidly at an increasing rate In...
... Depletion of cholesterol by pre-incubation of cells with MBCD and depletion of Ca2+ by thapsigargin and incubation in zero external Ca2+ resulted in attenuation of lysoPI induced exocytosis, indicating ... sustained increase in [Ca2+]i in PC-12 cells The lysoPI induced increase in [Ca2+]i was attenuated by pretreatment of cells with MBCD and by pre2+ incubation of cells with thapsigargin No change in ... transmission findings suggest that sPLA2 may have a rolein exocytosis and neurotransmitter release in neuroendocrine cells and neurons In recent findings (in chapter 4), it showed high levels of sPLA2-IIA...
... immunolabeling of sPLA2-IIA in the spinal trigeminal nucleus of the brainstem and dorsal horn of the cervical spinal cord is consistent with previous findings of an important roleof sPLA2 in nociceptive ... nicardipine (Arioka et al 2005) The contribution of sPLA2 in inflammation has been extensively studied Recent findings showed that sPLA2 induced expression of pro-inflammatory cytokines including ... showed bands at 14 kDa of sPLA2-IIA in homogenates from brainstem and spinal cord and a second band at approximately 30 kDa in the spinal cord The second band could be due to dimerization of sPLA2-IIA...
... region, indicating the nociceptive function of sPLA2-III The expression profile of sPLA2-III in CNS and its effects on exocytosis in rat PC-12 cells further illustrated the roleof sPLA2-III in pain ... mRNA and protein expression were expressed at the highest levels in the brainstem and spinal segments The enzyme was localized to dendrites in spinal trigeminal nucleus, supporting its rolein ... of sPLA2 isoforms mRNA and protein expression in rat CNS between previous reports and current findings ……………………… … 116 XI List of Figures LIST OF FIGURES SECTION I Figure.1.1 Site of action of...
... thapsigargin and recording in zero Ca2+ conditions, or treatment of cells with lanthanum chloride and recording in external solution containing Ca2+, resulted in attenuation of sPLA2-III induced ... anterior cingulate cortex which plays an important rolein the affective dimension of pain (Gong et al 2010), indicating the important roleof bee venom in nociceptive transmission The findings in this ... expression in the hindbrain Data represent the mean and standard deviation of four rats 115 Chapter Roleof Group IIA sPLA2 in nociception Table 2.4.1 Comparison of sPLA2 isoforms mRNA and protein expression...
... similar role to NO in wound healing in the rat 1.3 Thesis Overview and Scope The aim of the work presented in this thesis is to determine the roleof H2S in skin wound healing and the effect of PAG ... Chapter Introduction (2) Investigate the inflammation and wound recovery after PAG administration (3) Compare the H2S releasing profiles and outcomes of S-diclofenac administration and PAG incorporated ... system; inflammation in wound healing; DL-propargylglycine (PAG) and S-diclofenac (Ac-S)’s properties and effects as the inhibitor of H2S synthesizing and H2S donor; assembly of hydrogel in wound...
... protein 42 (Cdc42) and Par polarity complex protein, including Par3, Par6, and atypical protein kinase C (aPKC), may play crucial roles in the generation and controlling direction of polarity in ... filaments and induces growth of a new branch of actin filament at a unique 70 degree angle from the preexisting actin filament Proteins, such as profiling and ADF/cofilin, are also act as regulators in ... regulators in mediating actin polymerization by controling the availability of activated actin monomers, debranching and depolymerizing proteins and capping and severing proteins (Ridley et al.,...
... ligase and Ex Taq DNA polymerase were from Enzyme assay, SDS/PAGE and protein determination Acinetobacter strains and the recombinant E coli strains were cultured in mL LB medium For cultivation of ... proteins in the protein sequence database (see text for details) Arrows indicate the direction and extent of sequence determination led to the identification of a single recombinant clone harbouring ... The resulting plasmid, named pDHEB, contains the gene encoding DCH lacking the C-terminal region, in which Asp and His residues involved in the catalytic triad are contained The kanamycin resistance...