role of implantation of human stem progenitor cells
Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... removed by washing cells times, then G-CSF (R&D Systems, Minneapolis, MN) was added to cells at a final concentration of 200 ng/ml CFU assay of BM cells Tubes of MethoCult® GF M3434 (Stem Cell Technologies, ... GFP control cells was not detected When the number of infused cells was raised to × 105 cells, both Sall4A and Sall4B transduced cells repopulated of recipients, whereas GFP transduced cells did ... these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression of GR-1, CD4/8, B220 and Sca-1 in BM cells respectively...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells [12] Therefore, ... by Lipofectamine 2000, and then cells were exposed to either normoxia (20% O2) or hypoxia (10% O2) for days The percentage of GFP-positive cells was about 70% of that of total neural stem cells ... Long-term survival of human central nervous system progenitor cells transplanted into a rat model of Parkinson’s disease Exp Neurol 148, 135–146 Storch A & Schwarz J (2002) Neural stem cells and neurodegeneration...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... mobilization of cells expressing hematopoietic stem cell markers Data is presented as percentage of mononuclear cells in Additional File Analysis of the number of the progenitor cells in circulation ... Chandler AZ), was capable of increasing the number of circulating stem cells and progenitor cells This proprietary food supplement is produced by fermentation of a combination of green tea, astralagus, ... feasibility of significant mobilization of cells expressing hematopoietic stem cell and EPC markers and properties The area of nutritional modulation of the stem cell compartment offers significant...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... future here lies in implantation of hepatic stem cells, or in implantation of hepatic cells obtained by differentiation of a different type of stem cell, such as mesenchymal stem cells Other applications, ... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... the event of autologous implantation, but induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor cells ... Romagnani P, Maggi E, Romagnani S, Annunziato F: Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 D'Ippolito G, Schiller ... differentiation of human adult stem cells from bone marrow stroma (MSCs) J Bone Miner Res 2004, 19:256-264 De Bari C, Dell'Accio F, Tylzanowski P, Luyten FP: Multipotent mesenchymal stem cells from adult human...
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Báo cáo y học: "Translational control plays a prominent role in the hepatocytic differentiation of HepaRG liver progenitor cells" docx
... potential is often impaired and liver progenitor cells, also called oval cells, significantly increase both in number and their capability to proliferate [2,3] In recent years, liver progenitor cells ... differentiation of HepaRG cells HepaRG cells were induced to differentiate into morphologically and functionally mature hepatocyte-like cells Differentiated HepaRG cells showed features of normal hepatocytes, ... data mining Pipeline profiling of transcriptional and translational changes occurring during hepatocytic differentiation of HepaRG cells Figure for Pipeline for profiling of transcriptional and...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... (Table 1) Table The purity of human RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* ... Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of total ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte...
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Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx
... Number of cells × 105 iPLA2-VIA by treatment of cells with bromoenol lactone (BEL) [11,12] In INS-1 insulinoma cells, acute inhibition of iPLA2-VIA reduces the relatively high content of lysoPC of ... [BEL] (µM) B B Number of cells × 105 Number of cells × 105 12 10 0 12 24 10 [MAFP] (µM) 36 48 12 10 Time (h) 12 24 36 48 Time (h) Fig Effect of BEL on the growth of U937 cells (A) Dose– response ... shows that treatment of the cells with BEL induced a significant accumulation of cells in G2 ⁄ M and S, and a concomitant decrease of cells in G1, with respect to untreated cells In contrast, pyrrophenone...
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Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc
... are involved in human cancer, through the development and progression of several types of malignant tumor An essential role of uPA and c-met in the migration and proliferation of human hepatocellular ... levels of miR-23b and of uPA and c-met AB2 control cells, which did not express uPA and c-met, displayed a high level of miR-23b, whereas the most aggressive HCC cells, which produced high levels of ... expression level of miR-23b The differentiated HuH6 and HepG2 cells showed a different profile of uPA and c-met expression, as well as of miR-23b The HuH6 cells, with undetectable levels of uPA and...
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Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt
... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology, ... catalysis of NO oxidation can maintain uninterrupted fluxes of NO and oxygen to the interior of the protein molecule, formation of RNS inside the molecule, and transfer of the reaction products out of ... and the absence of a specific marker One of the general features of tumour cells consists of changes in their cell surface, which can be also reflected in the glycosylation pattern of their secreted...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... concentration of 2–5 × 106 of cells per 200–500 μl of medium After hours at 37°C, cells were placed in complete medium and analysed by flow cytometry between day and day 60 Transduction of CD34-pDC ... transducing human pDC generated in vitro from CD34+ progenitor cells [38] We evaluated here, whether cells fully differentiated into pDC could be transduced by rAAV of serotypes and 2, but also of serotype ... cells and AAV vectors are mainly episomal A single exposure of pDC to rAAV2/1, rAAV2/2 or rAAV2/5 led to very low levels of transduced cells ranging from around 3% to less than 1% of E-GFP+ cells...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were ... factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Figure Dose-response effect of growth factors on proliferation of human NP cells (10%FBS/F12) (n = 20) Cells were incubated with ... Figure The morphology of human NP cells under 1%FBS conditions The morphology of human NP cells under 1%FBS conditions 1%FBS, 10 μg/L TGF-β1, NP cells were similar to the normal NP cells with regular...
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Meeting Environmental Challenges: The Role of Human Identity docx
... indifference to the suffering of both individual non -human animals and the destruction of the nonhuman natural world (including other species and ecosystems) Human attitudes PART I: HUMAN IDENTITY AND ENVIRONMENTAL ... which aspects of the human psyche in general, and of human identity in particular, are especially important in determining humans’ responses to environmental challenges Our choice of the processes ... ASPECTS OF HUMAN IDENTITY Having established the environmentally problematic influence of self-enhancing, materialistic values, of in-group identities that separate humans from nature, and of the...
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Tài liệu tiếng anh tham khảo the moderating role of human capital management
... specifically, it offers a model of employee capability development The purpose of this model is to present a set of constructs and to outline a series of links that may potentially explain the human capital ... development of intellectual capital must take into consideration the role that both antecedents and moderators play in the development of human capital Too often, executives focus on the development of ... empirically validate the proposed model and to test a moderating role of human capital management practices, a survey of the current employees of a major North American financial services institution was...
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the role of human resource systems in job
... the meaning of human resource systems and applicant perceptions of person-organization fit Human Resource Systems The term human resource systems can be used to refer to the collection of policies, ... determinants of job acceptance Human Resource Systems The Role of Human Resource Systems in Job Choice Decisions For several years staffing experts have been suggesting that an organization's human ... RUNNING HEAD: Human Resource Systems The Role of Human Resource Systems in Job Choice Decisions Robert D Bretz, Jr and Timothy A Judge Center for Advanced Human Resource Studies School of Industrial...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
... overexpression of Bcl-xL is associated with radioresistance of human osteosarcoma cells In other human cancers, the association of Bcl-xL expression and radiosensitivity of tumor cells is also ... mock Figure.3 Effects of Ad/shBcl-xL on the proferation and colony formation of CRC cells A The proliferation of LoVo cells was measured by MTT assay The cell proliferation of LoVo infected with ... viability of LoVo cells was measured by a (Sigma, USA) Above three kinds of cells (5.0×103/well) were seeded into five 96-well culture plates with each plate having all three kinds of cells (6-parallel...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
... have been called ‘professional’ suppressor cells [5,8] In general, the properties of rodent and human CD4+ CD25+ T cells appear to be very similar In humans, 6–18% of CD4+ T cells constitutively ... CD4+ CD25+ cells have potent suppressive effects on CD8+ cells Rodent CD4+ CD25+ regulatory cells cause CD8+ cells to enter cycle arrest [46] The precursors of the human CD4+ CD25+ T cells induced ... small number of CD25+ 243 Arthritis Research Vol No Horwitz et al cells in the naive fraction Although
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Báo cáo y học: "Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts" doc
... presence of Th2 cells than in the presence of Th1 cells, whereas MCP-1 mRNA levels were similar (Figure 6a) To further document the capacity of polarized T cells to induce distinct patterns of chemokine ... capacity of T cells to elicit the production of chemokines by dermal fibroblasts and, in particular, the capacity of polarized T cells (Th1 and Th2) to modulate fibroblast production of chemokines ... presence of resting T cells (Figure 1a) Dose-dependent increases in IL-8, MCP-1 and IP-10 were observed in the presence of activated T cells However, Th1 cells were less potent inducers of IL-8...
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báo cáo khoa học: "Intracellular gold nanoparticles enhance non-invasive radiofrequency thermal destruction of human gastrointestinal cancer cells" ppt
... generator of heating of solutions of gold nanoparticles (GNPs) exposed to external radiofrequency (RF) fields at Thermographic results power outputs Thermographic results of heating of solutions of ... microscopy of Panc-1 cells treated Transmission electron microscopy of Panc-1 cells treated with 67 µM/L gold nanoparticles Panel 1: minutes of external radiofrequency (RF) field treatment Note loss of ... RF-treated cells comparing control cells (no GNPs) to cells with various concentrations of GNPs (data not shown) This suggests that heat release from GNPs in the microenvironment of the cells is...
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Báo cáo khoa học: "Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus" pdf
... discovery of cellular factors that is essential for mediating the infectious entry process of DENV into cells siRNA profiling of human membrane trafficking genes required for DENV infection An array of ... The advantages of this pooled approach as well as the issues of gene compensation of specific isotype of gene are discussed in [65] The list of 119 targeted human genes and isoforms (excluding ... regardless of the concentrations of the drug added to the cells (Figure 5B) Minimal cytotoxicity was observed for the concentrations of the drugs used in this part of the experiments Page of 17 Dominant...
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