role of fatty acid binding proteins in fatty acid metabolism differentiation and signal transduction
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... results seem to indicate that allosteric binding by GTP alone or in combination with UTP and ATP-cS, is not capable of activating glutamine hydrolysis in terms of kcat to the level of CTP synthesis, ... basis of the kinetic constants in Table 1, and those obtained for the glutaminase reaction in the presence of 0.1 mM each of UTP and ATP-cS (data not shown) Apparently, the value of Ka, kcat,1 and ... hydrolysis of glutamine has some aws in explaining the inhibition of DON-labeled enzyme when assayed with NH4Cl as amino donor Therefore, Levitzki and Koshland suggested that upon GTP binding, small structural...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... Table Role of nuclear actin -binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect ... Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking proteins Functions as scaffolds for signaling intermediates that stimulate actin elongation; binding partners ... central DNA -binding domain (DBD) and a C-terminal ligandbinding domain (LBD) containing activation function [67–70] Upon binding androgens, the AR LBD undergoes conformational changes leading to dissociation...
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Báo cáo Y học: The role of the second binding loop of the cysteine protease inhibitor, cystatin A (stefin A), in stabilizing complexes with target proteases is exerted predominantly by Leu73 pdf
Ngày tải lên :
17/03/2014, 10:20
... cystatin A in green and papain in blue Residues in the second binding loop of cystatin A mutated in this work are in red Papain residues involved in interactions with the cystatin A second -binding- loop ... second binding loops of cystatin B and family cystatins and also what residues of this loop in cystatin A may participate in the interaction To elucidate the role of the second binding loop of human ... importance of the second binding loop of cystatin A for the binding of cysteine proteases, in particular cathepsin B The role of this loop is comparable to that of the corresponding loops of cystatin...
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Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx
Ngày tải lên :
30/03/2014, 02:20
... Stability of cofactor binding site in SHMT cluster is not directly involved in the proper positioning or stabilization of the active site or PLP -binding residues Its location rather suggests a role in ... between the subunits and are involved in cofactor binding, substrate binding and catalysis (Fig 1) On the other hand, the mutations did not affect either the capability to bind the cofactor or the ... PLP -binding properties of the monomeric denaturation intermediate The cofactor binding properties of monomeric wildtype and mutant enzymes in 2.3 m urea (dissolved in the same buffer used in the...
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Báo cáo Y học: Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP+ reductase in the interaction with substrates pptx
Ngày tải lên :
31/03/2014, 08:20
... indicate that E139 is not involved in the processes of binding and ET between FNR and NADP+/H, while the nature of the charge and the conformation of the side-chain at position 139 of Anabaena FNR modulates ... this position is in some way involved in weakening the interaction between FNR and Fd In line with this, the conservative replacement of E139 by aspartic acid produces an increase in the K Fd m ... evacuation and flushing with O2free Air, before being introduced into the stopped-flow syringes Equimolecular concentrations of FNR and each of its substrates were used Final concentrations were kept in...
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báo cáo hóa học:" Assessing the clinical utility of measuring Insulin-like Growth Factor Binding Proteins in tissues and sera of melanoma patients" potx
Ngày tải lên :
18/06/2014, 15:20
... The role of the insulin-like growth factor binding proteins and the IGFBP proteases in modulating IGF action Endocrinol Metab Clin North Am 1996, 25(3):591-614 Rajaram S, Baylink DJ, Mohan S: Insulin-like ... factor binding protein-4 and its role in cancer (review) Int J Oncol 2006, 28(6):1317-25 Damon SE, Maddison L, Ware JL, Plymate SR: Overexpression of an inhibitory insulin-like growth factor binding ... (IGFBP-3): Insulin-like Growth Factor Binding Protein-3; (IGFBP-4): Insulin-like Growth Factor Binding; Protein-4; (MAPK): Mitogen-activated protein kinase; (IMCG): Interdisciplinary Melanoma...
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Báo cáo hóa học: " Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding" pot
Ngày tải lên :
20/06/2014, 01:20
... putative CD81 binding regions and are involved in CD81 binding as class I mutants (Y527A, W529A, Y613A, H617A and Y618A) (see Fig 4) demonstrate a defect in CD81 binding while maintaining proper ... implicating them as being directly involved in E2 binding to CD81 In conclusion, we have determined that the second and third putative CD81 binding regions are responsible for mediating E2 binding ... these regions as putative CD81 binding domains, the binding of the mutants to recombinant soluble CD81-LEL [4] was assayed In these experiments, binding of HCV E1E2 proteins to a purified GST tag...
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báo cáo khoa học: " The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization" potx
Ngày tải lên :
12/08/2014, 03:21
... these signals are often enriched in the basic amino acids lysine and arginine, two amino acids that are well represented in the carboxyl-terminal regions of AtPum23 and AtPum24 These two proteins ... exon-junction complex of proteins, RNA -binding proteins, and other proteins localize to nucleoli in plant cells supports a role for the nucleolus in mRNA processing, silencing, surveillance and export [44,45] ... resulting in an amino acid triplet that matches that of Puf repeats and in the conserved proteins Interestingly, this CRQ triplet is also found in repeat in some fungal and protozoan Puf proteins...
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Báo cáo y học: " Role of lysophosphatidic acid receptor LPA2 in the development of allergic airway inflammation in a murine model of asthma" potx
Ngày tải lên :
12/08/2014, 14:20
... receptors in linking lung injury in the murine bleomycin model of pulmonary fibrosis Asthma is a chronic inflammatory disease of the airways involving T-lymphocytes and eosinophils infiltration, ... structures Th1 and Th2 cytokines play a key role in orchestrating inflammatory and structural changes of the airway in asthma by recruiting, activating and promoting inflammatory cells into the airway ... neutrophil influx, protein leak, and E-cadherin shedding in bronchoalveolar lavage (BAL) fluids in a murine model of LPS-induced acute lung injury [27] These data suggest a protective role of administrated...
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Molecular analysis of oxysterol binding proteins in yeast
Ngày tải lên :
16/09/2015, 08:31
... 2.4.2 The role of Osh proteins in maintaining sterol homeostasis………………… .29 26 2.4.3 The role of Osh proteins in membrane trafficking…………………………………30 27 2.4.4 Roles of Osh proteins in other cellular ... 3.4.2 Determine the lipid ligand(s) of Osh6p ………………………………………… 44 39 3.4.3 Examine the role of Osh6p in maintaining sterol homeostasis ………………… 45 39 3.4.4 Investigate the role of Osh6p in membrane ... Oxysterol Binding domain; O.D: Optical Density; OPI1: OverProduction of Inositol; ORD: OSBP Related Domain; ORP: OSBP Related Protein in humans; OSBP: Oxysterol Binding Protein; OSH: OSBP Homologues in...
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The Role of Genetically Modified Organisms (GMOs) in Beverage Production
Ngày tải lên :
25/10/2013, 21:20
... mutagenesis, create signiÞcant changes in the genetic makeup of plants and animals due to the random recombination and sorting of thousands of genes As a result of intervention by people, the hybrid ... teosinte, the original ancestor of corn The newer techniques involving genetic engineering, on the other hand, allow for the transfer of a few genes in a much more precise, controllable, and ... Advantages of insect- and virus-resistant crops include improved yields and reduced use of pesticides An additional beneÞt of Bt corn is reduced contamination by fumonisin-producing fungi Fumonisin is...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
Ngày tải lên :
16/02/2014, 09:20
... E2F1, -2 and -3 These activating E2Fs cooperate with NF-Y proteins binding to CCAAT-boxes and with Myb proteins associating with a distal Myb site in activating the Cdc2 promoter Binding of the ... contains mammalian homologs of Caenorhabditis elegans synMuvB proteins and is composed of p130, E2F4 ⁄ and DP1 ⁄ and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatinassociated ... promoters In G0, proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting According to these early experiments all binding is lost in G2 ⁄ M In contrast, constitutive binding...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
Ngày tải lên :
19/02/2014, 05:20
... 2006 FEBS 369 Inhibition of choline -binding proteins B Maestro et al Thermal unfolding transition in the absence and in the presence of the different ligands The in uence of ligand binding on the ... useful to date for determining the structures of both Inhibition of choline -binding proteins the ligated and unligated forms of C-LytA, due to the insolubility of the protein at the required concentrations ... sites Finally, the accessibility Inhibition of choline -binding proteins of the alkaloids to new binding sites cannot be ruled out In this sense, the analysis of the three-dimensional structure of...
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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
Ngày tải lên :
22/02/2014, 04:20
... Zn(II) -binding activity by the test proteins represents the specific binding of Zn(II) Fig Dose-dependent binding of Zn(II) to purified E coli zinc -binding proteins The newly identified E coli Zn(II) -binding ... open reading frames) If the relative amount of Zn(II) -binding proteins in terms of total protein species is the same for other E coli proteins, the total number of zinc -binding proteins in E coli ... specific binding activity to Zn(II), but is not attributable to nonspecific metal binding Specificity of Zn(II) binding by the purified Zn(II) -binding proteins The specificity of Zn(II) binding was...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
Ngày tải lên :
06/03/2014, 11:20
... not only in Tyr349 Tyr347 Features of the amino acid sequence of the V spinosum tyrosinase Copper binding motif Copper binding motif Cys84 Arg40 bacterial Copper binding motif Copper binding motif ... (amino acids 86–96) and copper B (amino acids 258–294) binding motifs common to most tyrosinase sequences [3] that contain five of the six copper -binding histidine ligands The sixth histidine ... three domains: a twin arginine translocase (TAT) signal peptide, a core domain containing the two copper -binding motifs and a C-terminal extension (Fig 1B) The presence of a predicted TAT signal...
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Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
Ngày tải lên :
07/03/2014, 03:20
... large and small domains, respectively, and appear to stabilize their protein scaffolds, allowing the proper positioning of the residues involved in PLP binding, substrate binding and modulation of ... PLP binding properties The affinity of wild-type and mutant forms for the cofactor was measured to evaluate the impact of the mutations on the structure of the PLP binding site Because PLP binding ... saturating concentrations of cofactor (Finf) were: F0 = 125 ± and Finf =65 ± for the wild-type enzyme; F0 = 139 ± and Finf = 66 ± for L85A; F0 = 122 ± and Finf = 63 ± for L276; and F0 = 103 ± and Finf...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
Ngày tải lên :
07/03/2014, 16:20
... Nonspecific binding was determined in the presence of lm unlabeled agonist and subtracted from the total binding to calculate the specific binding Internalization of [3H]BK and [3H]DAK To determine the internalization ... drop in surface binding that could not be accounted for by the amount of internalized agonist [20] As the internalization is expressed in percentage of total binding, decreasing the binding affinity ... arrangement of the amino acid residues This arrangement is probably important in Role of helix and C-termini in bradykinin receptors receptor signaling because: (a) interruption of the amphipathic...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
Ngày tải lên :
08/03/2014, 08:20
... sufficient for inducing activation of GS in muscle and fat cells, and that inhibition of the kinase potentiates insulin action in muscle of insulin-resistant rats [39], and that prolonged GSK3 inhibition ... enhanced in insulin-resistant muscle and the kinase has been implicated in down-regulating insulin signalling via its ability to serine phosphorylate IRS1 [40], it is possible that inhibition of GSK3 ... coordinate increases in glucose uptake and glycogen synthesis allowing for more effective ÔchannellingÕ of glucose into glycogen in response to insulin However, one of the potential difficulties in...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
Ngày tải lên :
15/03/2014, 09:20
... reflect VnfA of the nucleotide binding form It has been reported that binding of ATP or ADP to NifA of A vinelandii leads to rearrangement of interaction between the GAF and AAA+ domains (and thereby ... conformations of VnfA corresponding to a combination of the presence and absence of AMP-PNP and the Fe-S cluster (vide infra) This could help to solve the problem of why binding AMP-PNP has an in uence ... proteolysis of VnfA, a similar effect of the nucleotide binding was reported in a study of the limited trypsin digestion with NifA + MgADP, in which binding MgADP to the central AAA+ domain is ascribed...
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Báo cáo khoa học: Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence doc
Ngày tải lên :
16/03/2014, 02:20
... of proteins, with R proteins in the largest family containing nucleotide -binding sites (NB) and leucine-rich repeat (LRR) domains [38,39] The N-termini of these proteins display either Toll and ... caspases Interestingly, the mycotoxin and sphingosine analogue fumonisin-B1 is a powerful inducer of singlet oxygen-dependent PCD in animals and plants [115,116] Fumonisin-1-induced PCD in plants ... shares amino acid sequence homology to that of ribosome-inactivating proteins (RIPs) found in bacteria and plants [69,70] The N-terminal half of the novel barley RIP is related to both type I and...
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