rna seq analysis of gene expression and alternative splicing by double random priming strategy
Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... Criteria for cell synchronization and analysis of gene expression 8) The size distribution of newly synchronized cells should be narrower than the size distribution of the original population, cell ... synchronized Synchronized divisions are the sine qua non of synchrony Criteria for successful analysis of gene expression during the division cycle 12) Gene expression results should be replicated (with ... different experiments in terms of variation in the phase angles of peaking of gene expression in different individual cells Thus, given a particular variation in gene expression in different cells,...
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Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx
... gene expression [6-8] In contrast, the duration and nature of the agonal state preceding death appear to have a substantial effect on gene expression by affecting the integrity of messenger RNAs ... sets that differ in expression between autopsy and resection samples and for 1,000 subsets of the same size randomly sampled from the other 14,624 probe sets In 837 out of 1,000 random subsets, the ... autopsy and biopsy samples found significant change in one of the genes, indicating that a substantial proportion of all expressed genes could be affected by death [10] We surveyed gene expression...
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Analysis of gene expression
... for production of either the sense or the antisense RNA strand of the sequence by using T7 RNA polymerase These RNA samples are then mixed and annealed to give double- stranded RNA that can be ... identification of gene function (24) The basic mechanism of RNAi involves introducing a double- stranded RNA (dsRNA) of around 300–500 bp into a cell by microinjection or feeding This dsRNA molecule ... integrity of samples, and to ensure that normal- Analysis of gene expression 193 T7 RNA polymerase Primer Competitor DNA template Primer In vitro transcription by T7 RNA polymerase Target RNA Competitor...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... al (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression ... number of serial analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene ... gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... 5428 13 14 15 16 17 18 19 20 Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression Hum Reprod 21, 1583–1590 Matsumura ... 2008 The Authors Journal compilation ª 2008 FEBS 5425 New method of 3¢-end amplification from SAGE tags W.-J Xu et al database (refseq _rna: reference mRNA sequence and expressed sequence tags) revealed ... serial analysis of gene expression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense transcription of key pluripotency...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... Table Sequence of DNAzymes and site of cleavage of uPAR mRNA transcript The catalytic core sequence is underlined The corresponding mutant control DNAzyme for each was made by scrambling the sequence ... substrate, and a maximum reduction of 84% of uPAR substrate concentration at a molar ratio of : 200 (Fig 2D) The specificity of the DNAzymes was confirmed by the lack of cleavage by the untreated and...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... architecture; and proteoglycan staining characteristics Statistical analysis Relative levels of gene expression were determined using Q -Gene[ 38] and the housekeeping gene GAPDH as an internal standard ... consisted of μl of diluted cDNA, 0.3 μM of forward and reverse primers (1 μl each), 10 μl of the 2X QuantiTect™ SYBR® green master mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free ... and the column was washed three more times RNA was eluted off the column using 30 μl of RNase free water The yield of extracted total RNA was determined by meas- uring absorbance at 260 nm, and...
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Báo cáo y học: "Microarray analysis of gene expression in lupus" ppt
... studies as a measure of diagnosis or clinical outcome [11] Microarray analysis of gene expression at sites of tissue damage The next generation of reports describing global gene expression patterns ... muscle by using immunohistochemistry The authors interpreted their data as consistent with effects of both IFN-α/β and IFN-γ on gene expression In addition to this set of IFN-induced genes, gene expression ... blood of patients with SLE [3–6] Most striking is an ‘IFN signature’, a prominent overexpression of mRNAs encoded by genes regulated by IFNs and similar to that detected in JDM muscle Detection of...
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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
... mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA cartilage determined by ... in situ effect of licofelone on the gene expression and protein synthesis of the major collagenolytic enzymes (MMP-13 and cathepsin K) and aggrecandegrading proteases (ADAMTS-4 and ADAMTS-5) in ... negative and showed only background staining Treatment with licofelone had little effect on the level of its gene expression or on the level of protein In contrast, the level of expression of mRNA...
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báo cáo khoa học: " Analysis of gene expression in response to water deficit of chickpea (Cicer arietinum L.) varieties differing in drought tolerance" pps
... clustering tree (B), expression profiles of SOTA clusters The expression profile of each individual gene in the cluster is denoted by grey line and the mean expression profile is depicted by pink line ... Page of 14 Figure Hierarchical clustering analysis of 53 selected genes based on their gene expression patterns Analysis of expression profiles of 53 ESTs (Additional File 4) in PUSABGD72 and ... Average expression intensity of the cluster genes was much higher than that of the cluster genes and there was uniformity in the expression of the cluster genes Two ESTs [FL512394 and FL518992] of...
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Báo cáo y học: " Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P" pptx
... inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA RNA 2006, 12:63-72 29 Yang YH, Li H, Zhou T, Kim K, Liu F: Engineered external ... target RNA (e.g., mRNA) and an EGS that resembles the structure of a tRNA (C-F) Complexes between UL49 mRNA sequence and EGS324, C-EGS324, EGS1539, and CEGS1539 respectively The sequence of these ... studies of targeting activities of the EGSs The EGSs were subsequently incubated with human RNase P and substrate RNA, containing the targeted UL49 mRNA sequence of 1713 nt In the absence of any...
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báo cáo khoa học: " Analysis of gene expression in cotton fiber initials" pptx
... supported by histological methods and more detailed analysis of expression of selected genes to broaden our understanding of cotton fiber development Results Isolation of RNA from dpa fiber One goal of ... fiber The agreement of the known expression of these genes with expression of these genes on microarrays validated the microarray results Global analysis of gene expression Genes that vary greater ... meaning of the changes of expression of large sets of genes [28] Gene ontologies are definitions of genes using a well defined species-independent vocabulary GO were not available for most of the genes...
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Báo cáo y học: "New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model" potx
... surface expression of these genes Comparing the gene profiling of G3 versus G2, six genes stood out in our analyses (Table 3) Although G3 animals have not developed ANFH, their gene profile reflects ... Here we employed gene profile analysis using this susceptible rat strain in order to study the pathogenesis at an early disease stage Knowledge of the gene expression pattern and the events that ... contribute to the genesis and progression of ANFH in this rat model could provide a better understanding of the pathogenesis in humans Materials and methods Experimental animals and their maintenance...
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Báo cáo y học: ":Identification of novel stem cell markers using gap analysis of gene expression data" ppsx
... number of genes for a Gene Ontology (GO) category in the unselected marker set; N2 and P2 are the number of genes and P value for the smaller marker set; N3 and P3 are the number of genes and P ... following gene duplication Phylogenetic analysis of one example from each family (Figure 5) led to the study of the evolution of a total of 13 genes An ancestral gene existed for each of the four ... marker genes from the results generated with k-means, a subsequent analysis of the expression profile in each k-means cluster would be required to identify the samples in which the cluster of genes...
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Báo cáo y học: "Analysis of gene expression in operons of Streptomyces coelicolor" ppsx
... intra-operonic and non-operonic gene expression Correlations in intra-operonic and non-operonic gene expression Correlations in intra-operonic and non-operonic gene expression in (a) S coelicolor and (c) ... control, when selecting the same number of genes randomly from all of the documented S coelicolor operons and randomly splitting them into upstream and downstream genes (following 1,000 simulations) ... internal promoters Variation up and down stream of putative internal promoters Box plots of the mean expression of genes upstream and downstream of the putative internal promoter in (a) documented...
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Báo cáo y học: "Analysis of gene expression in a developmental context emphasizes distinct biological leitmotifs in human cancers" pdf
... time Analysis of differential gene expression and construction of developmental profiles Differential gene expression between tumors and corresponding controls was determined using significance analysis ... 10.0 9.2 Gene expression developmental time course Naxerova et al R108.4 9.8 Gene2 9.6 Upregulated Gene4 Gene5 9.4 Expression 8.8 8.6 Expression 9.6 9.4 Expression 9.8 9.0 Gene3 9.0 Gene6 9.0 ... and tested the enrichment of approximately 1,000 gene sets in the up- and downregulated genes of our data sets Subsequently, we compared the results with the performance of eDEV500, lDEV500 and...
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Báo cáo y học: " Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression" pptx
... methodology for global analysis of transcriptomes is serial analysis of gene expression (SAGE) This sequencingbased technique generates 14-bp sequences (tags) to evaluate thousands of transcripts in ... Serial analysis of gene expression Science 1995, 270:484-487 Wahl MB, Heinzmann U, Imai K: LongSAGE analysis significantly improves genome annotation: identifications of novel genes and alternative ... quantification of RNA amounts This SAGE experiment cannot be considered a comprehensive study of gene expression during the early development of X tropicalis because of the low sequencing level of our...
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Book Econometric Analysis of Cross Section and Panel Data By Wooldridge - Overview pps
... available in Volume of the Handbook of Econometrics—see Powell (1994) and Hardle and Linton (1994)—as well as in ¨ Volume 11 of the Handbook of Statistics—see Horowitz (1993) and Ullah and Vinod (1993) ... can improve on asymptotic analysis Volume of the Handbook of Econometrics and Volume 11 of the Handbook of Statistics contain nice surveys of these topics (Hajivassilou and Ruud, 1994; Hall, 1994; ... E¤ects Models by Pooled OLS Random E¤ects Methods 10.4.1 Estimation and Inference under the Basic Random E¤ects Assumptions 10.4.2 Robust Variance Matrix Estimator 10.4.3 A General FGLS Analysis 10.4.4...
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Book Econometric Analysis of Cross Section and Panel Data By Wooldridge - Chapter 1 pot
... important is lost by treating explanatory variables that are set ahead of time as being random It is safe to say that no one ever went astray by assuming random sampling in place of independent ... assumptions involving things such as the number of absolute moments of a random variable that must be finite.) For much of this book we adopt a random sampling assumption More precisely, we assume ... saving of employees within a firm may be correlated because of common (often unobserved) characteristics of workers within a firm or because of features of the firm itself (such as type of retirement...
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Book Econometric Analysis of Cross Section and Panel Data By Wooldridge - Chapter 2 pdf
... expectation operator, and several additional properties that are consequences of the randomness of mðxÞ Some of the statements we make are proven in the appendix, but general proofs of other assertions ... equation (2.47) generally more useful than equation (2.48)? 2.4 For random scalars u and v and a random vector x, suppose that Eðu j x; vÞ is a linear function of ðx; vÞ and that u and v each have ... book Suppose that w is a random vector and y is a random variable Let x be a random vector that is some function of w, say x ¼ fðwÞ (The vector x could simply be a subset of w.) This statement implies...
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