rna rna interactions in the spliceosome
Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... domain or full-length MSR results in a quenching of the intrinsic flavin fluorescence, suggesting that the flavin chromophore is shielded from the solvent in the protein–protein complex [19] From the ... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... footnote) in the reaction mixture may reduce a proportion of the FMN domain, converting some of the enzyme to the active form The 3-fold increase in activity may arise from the binding of the FMN...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
... antitumor RNases the dimer interface of the models or in the surroundings of the hinge loop residues Likewise, the models of nonexchanging and exchanging HHP-RNase were prepared starting from 1Z7X The ... dimer interface of the models or in the surroundings of the mutated residues The models of RNase A covalent dimers in the exchanging conformation were prepared using the 7RSA structure and the ... modified RNase Structure pI IC50 (lgÆmL)1) RNase A RNase-AA RNase-AA-G RNase-AA-GG HP-RNase HHP-RNase BS-RNase Bovine pancreatic RNase A A19P,Q28L,K31C,S32C-RNase A D38G-RNase-AA D38G,E111G-RNase-AA...
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Managing Drug Interactions in the Treatment of HIV-Related Tuberculosis docx
... available The Role of Rifamycins in Tuberculosis Treatment Despite the complexity of these drug interactions, the key role of the rifamycins in the success of tuberculosis treatment mandates that the ... rifabutin dose was decreased in response to antiretroviral therapy then stops taking the interacting drug (e.g., ritonavir), the resulting rifabutin concentrations are likely to be sub-therapeutic These ... in drug-metabolizing enzymes Therefore, drug interactions and their relevance may not be the same in different populations Third, in the attempt to provide the most up-to-date information we include...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... agonist-induced alterations in BRET signal intensities reflecting 7TM dimer rearrangement as a result of the activating twist in the ATDs of the CaR homodimer Further investigations into the signal ... However, as these fusion proteins were retained in vesicles inside of the cells, we were not able to use them in the BRET studies It would have been interesting to tag Rluc and GFP molecules to the i2 ... agonist-induced BRET observed in this study is that the translation of agonist binding to the ATDs of the family C GPCR homodimer into G-protein coupling of the 7TM)7TM moiety is mediated by another...
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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot
... confirming the flexibility around the C6-C9 and N6-C10 bonds to adapt the position of the pyrrole rings to fit approximately parallel to the walls of the minor groove in their own region The terminal ... However, it is interesting that the guanine amino group may be in uenced by this contact We compared the interaction energies in both a planar and a nonplanar guanine G9 amino group The HF interaction ... in the planar guanine amino group was computed to be 55 kJÆmol)1 (40 kJÆmol)1 in the nonplanar guanine amino group), 25 kJÆmol)1 in total interaction energy (7 kJÆmol)1 in the nonplanar guanine...
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Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf
... with the adenylation domain at the base, and the UFD and cysteine domains on both sides The catalytic cysteine residue lies in a long loop between the cysteine domain and the adenylation domain ... for E2–E3 interactions (c) In the SUMO-1–ubc9–RanBP2–RanGAP1 (Ran GTPaseactivating protein 1) complex, the four-stranded b-sheet domain of ubc9 provides the binding site for the IR1 (internal repeat ... Ala72 in Nedd8, and Arg72 in ubiquitin (highlighted in yellow in Fig 2C) Matching E1 residues are Thr149 in uba2, Arg190 in uba3 and Gln608 in uba1 (highlighted in purple in Fig 3E) Interestingly,...
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Báo cáo hóa học: " Atomic Force Microscopy Study of Protein– Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System" docx
... r(h)AdR/Ad for binary mixtures (d) Arrows (1) indicate the images of protein monomers Arrows (2) indicate the images of the binary protein complexes Arrows (3) indicate the images of the ternary CYP11A1/AdR/Ad ... higher hmax than the ones of individual AdR and Ad In the binary mixture, the share of these objects in the positive wing of the Δr = (r(h) AdR+Ad [r(h)AdR + r(h)Ad]) distribution with the height 1.8–2.6 ... and dried in airflow The binary complexes were obtained by mixing 10 μl of μM solutions of appropriate individual proteins in 50 KP, pH 7.4 Then, the mixture was incubated for 10 min, diluted...
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THE IMPORTANCE OF BIOLOGICAL INTERACTIONS IN THE STUDY OF BIODIVERSITY pdf
... has been divided in three sections correponding to the three main kinds of biological interactions: interactions between living organisms (Section 1), interactions between living organisms and ... parameters as shown in Hawaii with the increase in the altitudinal range of Cx quinquefasciatus and the differences in vector competence to avian malaria after the second introduction of the vector (Fonseca ... states that the term biodiversity comprises all the variety of life, in all its manifestations, at all its levels of organization, and including their complex interactions The biological interactions...
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Motion and Gesture Interactions in the digital age
... Motion & Gestures interactions in the digital age From the first personal computing with Static interface and physical controls Motion & Gestures interactions in the digital age To an incredible new ... visualize the effect of a specific interaction in the digital space Motion & Gestures interactions in the digital age Introduction Motion Design & Digital products Web 1.0: Java & Gif At the beginning ... and motion interactions was the right answer to show a new kind of affordance Physical Motion patterns becomes virtual interactions Motion & Gestures interactions in the digital age Introduction...
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Báo cáo y học: "The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis" potx
... is indicated by the resorptive defect in osteoclast-mediated bone resorption in mice in which either of these genes has been deleted [36,37] In humans, inactivating mutations in the cathepsin ... particles expressing cathepsin K mRNA were detected in all of the tissues examined, although the mRNA levels were much lower in cells associated with polyethylene than in cells on the bone surfaces ... different integrins, including the vitronectin receptor αvβ3 [2,3] Expression of this integrin has served as a useful marker to identify osteoclasts and to distinguish them from their colony forming...
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Báo cáo y học: "CXCR3/CXCL10 interactions in the development of hypersensitivity pneumonitis" pot
... divided into four groups based on the positioning of the cysteine residues in the mature protein [36], induce directional migration of immune cells through their interactions with G-protein coupled ... Figure scheme of the effect of the CXCR3/CXL10 interactions in the lung of hypersensitivity patients Putative Putative scheme of the effect of the CXCR3/CXL10 interactions in the lung of hypersensitivity ... demonstrated in the lung of HP patients with T cell alveolitis Whether the presence of the two chemokines is concomitant and there are common molecular mechanisms involved in the CC and CXC chemokine...
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Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
... electrostatic interactions [60] (d) For the Transient RNA protein interactions in RNA folding human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding ... interactions within the RNA molecule The protein thereby smooths the folding energy landscape The direction of the RNA folding reaction (either annealing or displacement) is then no longer kinetically ... determines the magnitude of peptide RNA binding, owing to counterion release from the RNA backbone [66] The resulting entropy increase of the system drives binding of the peptide to the RNA (and...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... with valine, the ˚ cavity volume increased to 43 A3, reflecting a reduction in ˚ the surface area of the residue (% 59 A2) and in the interactions occurring there (Fig 8) Met88 resides in the 142 ... resides on the surface of the protein and makes no obvious stabilizing interactions with other amino acid sidechains, this part of the protein interacts with genomic RNA [33,41,47] Such interactions ... with valine (C and D), the cavity volume increased to 43 A3, reflecting a reduction in the surface area of the residue (% 59 A2) and in the interactions occurring there (corresponding to the b-sheet)...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... obtained with the wild-type (–)IRES in the absence or in the presence of heparin (The addition of heparin during RNA synthesis reduced RNA synthesis by 72%) Data were corrected following the ... studied RNA synthesis by recombinant HCV NS5B using deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus -RNA, the ... the 3¢-end Furthermore, we have reported that an oligonucleotide complementary to the SL-B1 domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... processes in a cell type-dependent manner For instance, an increase in the cell cycle timing could be expected following the downregulation of PCNA, coding for a protein involved in the control ... The role of sequence pairing in siRNA side effects is also supported by data showing that these effects can be dramatically reduced in the presence of another control, the RNA- induced silencing ... siRNAs targeting the lysosome-associated membrane protein (LAMP2) mRNA encoding a lysosomal glycoprotein, and a nontargeting (NT) siRNA Gene expression profiles were determined for each siRNA in...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... binding domain found in the splicing factor 45 and other DNA-binding proteins [11,20], whereas RRM (RNA recognition motif) was already described in numerous proteins [21,22] Further analyses of the ... with other proteins specialized in DNA repair Interestingly, they also contain motifs typical of proteins involved in RNA metabolism TgDRE mRNA and protein expression have been evaluated using ... protein expressed in the two developmental stages of T gondii To investigate whether these mRNA abundances increase the expression of TgDRE protein, a GST fusion protein consisting of amino-acid...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... when the yeast-tRNA was omitted from the binding assay, indicating a lesser specific binding to RNA Finally, the two truncated proteins containing a single PPR domain did not show any binding to RNA ... no binding takes place In order to verify the binding properties of HCF152, we analyzed the binding of these RNAs using the UV-crosslinking competition method In this method, only a single RNA ... analyzed in the UV-crosslinking competitive assays, as shown in D The IC50 of each RNA was determined by plotting the data (D) The location of the high affinity binding site in the Dd120 RNA defined in...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... with C1 The intramolecular interaction might be involved in regulating binding between NP and VP35 (binding is enhanced in the absence of C2) and is essential for the function of NP in transcription ... GST-VP35 B Binding to GST-VP35 (%) We then investigated whether the integrity of the coiled coil region of NP is important for the binding of NP to the polymerase cofactor VP35, which connects the polymerase ... increase the binding of GST-VP35 to the NP mutant (Figs 3B and 3C, ∆C2) These data indicate that the coiled coil region of NP influences the interaction with VP35 NP kDa 97,5 66 C2 A C1 the interaction...
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Báo cáo sinh học: " The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon" doc
... used Furthermore, control vectors devoid of an IRES in the intercistronic region or carrying the inhibitory stemloop in the 5'UTR of the dicistronic mRNA were included into the experiments The cell ... expression than the poliovirus IRES Including the extension into the coding region into the SD-1 IRES resulted in 1.5–3 fold increase in translational efficiencies irrespective whether the cell line is ... Surprisingly, Herbetreau and co-workers found, the HIV-2 RNA contains a new type of IRES which is located within the coding region [39] Another interesting result of our investigation was the finding...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... were then held in the thermocycler at the indicated temperatures for the indicated times prior to RTPCR At the end of the incubation, the thermocycler was programmed to ramp the samples at the ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing ... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation...
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