regulation of pericyte differentiation

Regulation of chondrocyte differentiation potential involvement of wnt ß catenin signaling

Ngày tải lên : 16/10/2015, 15:38

... 9: Redifferentiation ability of FBS-expanded P1 chondrocytes 51 Figure 10: Re -differentiation ability of P0 monolayer-expanded chondrocytes 54 ix Figure 11: Redifferentiation ability of ... caused the inhibition of collagen II and the onset of collagen I expression in chondrocytes, the typical symptoms of chondrocyte dedifferentiation Wnt 7a caused the suppression of collagen II by ... exposed to a cocktail of growth factors (TFP) and the Wnt/β-catenin antagonist, curcumin A control consisting of commonly-used FBS medium was included Redifferentiation ability of the cells that...

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Báo cáo khoa học: Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells pptx

Ngày tải lên : 23/03/2014, 17:21

... Transcriptional regulation of human DNase II by Sp1 and Sp3 (Eur J Biochem 270) 1859 Fig Western blot analysis of Sp1 and Sp3 in nuclear extracts of HL-60 cells Twenty micrograms of nuclear extracts ... Coaddition of the two antibodies resulted in the loss of bands C1, C2, and C3 (lane 13) In contrast, the use of a control monoclonal antibody against SREBP did not affect the formation of any of the ... lgÆlL)1 of poly dI-dC, 160 lgÆmL)1 of BSA, 0.008% NP-40, and protease inhibitor) Competitors [either a 10- or 50-fold excess of unlabeled wild-type or mutant probe or a 0.6- to threefold excess of...

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Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc

Ngày tải lên : 18/06/2014, 19:20

... design of the study and the analysis of the results Author details Division of Clinical Onco-Immunology, Ludwig Center for Cancer Research of the University of Lausanne, Switzerland 2Department of ... acts downstream of IL-15 in the regulation of memory CD8 T-cell homeostasis Blood 2006, 107:3992-3999 26 Sandalova E, Wei CH, Masucci MG, Levitsky V: Regulation of expression of Bcl-2 protein ... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA levels...

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báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx

Ngày tải lên : 20/06/2014, 03:20

... design of the study and the analysis of the results Author details Division of Clinical Onco-Immunology, Ludwig Center for Cancer Research of the University of Lausanne, Switzerland 2Department of ... acts downstream of IL-15 in the regulation of memory CD8 T-cell homeostasis Blood 2006, 107:3992-3999 26 Sandalova E, Wei CH, Masucci MG, Levitsky V: Regulation of expression of Bcl-2 protein ... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA levels...

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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Ngày tải lên : 09/08/2014, 10:20

... involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of ... involvement of β-catenin in Wnt regulation of MSC self-renewal Page of 10 (page number not for citation purposes) Arthritis Research & Therapy Vol No Kolf et al MSCs from a variety of mammalian ... chondrocytes and osteoblasts Specificity of lineage differentiation can also result from the recruitment of master transcriptional switches with binary regulation of cell fate, such as TAZ (transcriptional...

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IDENTIFICATION OF A MINIMAL CIS-ELEMENT AND COGNATE TRANS-FACTORS REQUIRED FOR THE REGULATION OF RAC2 GENE EXPRESSION DURING K562 CELL DIFFERENTIATION

Ngày tải lên : 24/08/2014, 12:22

... Transcriptional regulation of genes Genomic DNA is the ultimate template of our heredity In spite of the completion of the human genome project, many challenges remain in understanding the regulation of genetic ... melting of the DNA TFIIH has kinase and helicase activity The kinase activity of this factor is required for the phosphorylation of the C-terminus domain (CTD) of Pol II that consists of repeats of ... Construction of Plasmids 43 Construction of 5’- deletion constructs of the 4.5 kb human Rac2 promoter 43 Construction of the EF1α/luc and -30bp+135/luc constructs 43 Construction of the...

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Regulation of S Phase

Ngày tải lên : 25/10/2013, 21:20

... initiation (Fig 1) The chromatin loading of MCM2-7 depends on the loading of Cdt1 and Cdc6, which in turn depend on the loading of ORC This step- Fig Regulation of the pre-RC The pre-RC functions to ... is also co-dependent with Cdc45, Dpb11, and Sld3; binding of any of Regulation of S Phase 47 these proteins depended on the presence of the others (Kubota et al., 2003; Takayama et al., 2003) ... characterization of Xlinked mutants of Drosophila melanogaster which are sensitive to mutagens Genetics 84:485–506 Brown GW, Kelly TJ (1999) Cell cycle regulation of Dfp1, an activator of the Hsk1...

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Regulation of navigation and vessel-source pollution in the Northern Sea Route - Article 234 and state practice

Ngày tải lên : 01/11/2013, 09:20

... accompaniment of the CCGS John A 40 41 42 43 See Arts and 10 of the NSR Regulations; Arts 14 and 15 of the Economic Zone Edict; Arts and 15 of the Environmental Edict; and Art 11 of the Decree of the ... the Decree of the Council of Ministers of the USSR, June 1990, ‘On Measures of Securing the Implementation of the Edict of the Presidium of the USSR Supreme Soviet of 26 November 1984 “On Intensifying ... the NSR Regulations, as stated in Article (quoted above), include regulation of navigation, on the basis of non-discrimination These regulations are to apply to all vessels, regardless of nationality,84...

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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx

Ngày tải lên : 14/02/2014, 19:20

... repression of their target genes Indeed, several examples of feedback regulation that involve miRNAs have been reported Below, we attempt to summarize our recent understanding of feedback regulation of ... ﬁnding that TPAinduced upregulation of miR-34a depends on the activation of the ERK signal cascade and that miR-34a downregulates MEK1, which is one of the main regulators of ERK signaling, indicates ... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa This mechanism of regulation provides a wide range of coordinated...

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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

Ngày tải lên : 14/02/2014, 22:20

... direct dephosphorylation of Ser112 and negative regulation of the ERK pathway via p38 MAPK, both of which lead to impaired phosphorylation of Ser112 [70] After dephosphorylation of Ser112, Ser136 becomes ... translocation of Bax and Bad to mitochondria independently of caspase activation [77,87] Phosphorylation of caspases Phosphorylation of caspases switches the cellular apoptotic signal on and off Caspase ... regulating the activity of an isoform of PP1, PP1a [3] Similarly, activation of caspase-8 and -3 is regulated through their phosphorylation and dephosphorylation Phosphorylation of caspase-8 at Tyr397...

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Tài liệu Regulation of cancer cell metabolism docx

Ngày tải lên : 15/02/2014, 04:20

... splicing The inclusion of exon in the PK mrnA leads to translation of the PKM1 isoform, whereas inclusion of exon 10 produces PKM2 (ReF 71) MyC upregulates the expression of heterogeneous nuclear ... required for the assembly of the cytochrome c oxidase complex of the electron transport chain61 Thus, the loss of p53 might also be a major force behind the acquisition of the glycolytic phenotype ... Publishers Limited All rights reserved REVIEWS of genes that increase glucose metabolism and reduce mitochondrial respiration One of these genes encodes an isoform of PDK (PDK4) that has the same function...

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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

Ngày tải lên : 16/02/2014, 09:20

... general context of a promoter appears to be relevant because inversion of the site in the human Cdc25C promoter resulted in a deregulation of cell cycle-dependent transcription Deregulation is ... promoters, mutation of the CHR leads to a more pronounced deregulation than the alteration of the CDE [40] Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein ... investigating downregulation of the cyclin B2 promoter is that after deletion or mutation of constitutively activating sites, such as the destruction of CCAAT-boxes, small levels of reporter activity...

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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

Ngày tải lên : 16/02/2014, 09:20

... genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... FEBS 901 AP-2 proteins in cartilage differentiation A.-K Wenke and A.-K Bosserhoff 64 Huang Z, Xu H & Sandell L (2004) Negative regulation of chondrocyte differentiation by transcription factor...

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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Ngày tải lên : 18/02/2014, 04:20

... modiﬁcations of HSFs in different tissues and organs during development of an organism or differentiation of certain cell types This lack of knowledge severely hampers the understanding of the physiological ... strict regulation becomes necessary for the correct expression of HSF2 target genes Indeed, when miR18-mediated regulation of HSF2 was disrupted in male germ cells in vivo, expression of HSF2 ... HSEs, the Regulation of HSFs HSFs regulate different types of target genes that are involved in a broad range of cellular processes Therefore, the expression and activity of HSFs need to be under...

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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

Ngày tải lên : 18/02/2014, 08:20

... the regulation of miR-27 expression Our data suggest that the miR-27 gene family is potentially an important class of negative regulators of adipogenesis and may play a role in the regulation of ... inhibits adipogenic differentiation In order to investigate the role of miRNAs in the regulation of adipogenic differentiation, we performed a genome-wide microarray analysis of miRNA expression ... myogenic differentiation Taken together, these results illustrate a critical and speciﬁc role of miR-27 in the regulation of adipogenic differentiation miR-27 prevents the induction of PPARc...

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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Ngày tải lên : 18/02/2014, 11:20

... molecular weights of 161.8, 28.2 and 13.2 kDa, respectively Regulation of cathepsin B activity The formation of the cathepsin B ⁄ cystatin C complex was seen as a shift of the elution peak of cathepsin ... the presence of various concentrations of 2A2 mAb The binding of 2A2 mAb was followed by native gel electrophoresis Increasing the concentration of 2A2 mAb at a ﬁxed molar ratio of cathepsin ... the conformational changes of the active site of cathepsin B, the results obtained in the present study provide a speciﬁc mechanism for the regulation of the activity of cathepsin B, which can...

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Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

Ngày tải lên : 18/02/2014, 14:20

... level of MLL1 mRNA Interestingly, upon downregulation of MLL1, E2-mediated upregulation of HOXC13 was slightly decreased (Fig 4A, lane 3) Similar results were observed for MLL2 and MLL4 downregulation ... ERE2, ERE3 and ERE4 of the HOXC13 promoter As seen in Fig 5A, no significant binding of actin was observed in any of the EREs, irrespective of the absence and presence of E2 Binding of ERa and ERb ... amount of binding of MLL1 was observed in ERE3, no significant E2-dependent binding of any of the MLLs was observed in ERE3 and ERE4 (Fig 5A, lanes 5–8) Significant amounts of constitutive binding of...

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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Ngày tải lên : 18/02/2014, 16:20

... with dCTP (grey) and dTTP (yellow, cyan and magenta) (B) Stereoview of a superposition of one of the subunits of the E138A variant of E coli dCTP deaminase in complex with dTTP (yellow; chain B), ... Mutational analysis of amino acid residues involved in dTTP regulation of dCTP deaminase The design of the mutant enzymes H121A and V122G was inspired by the results from analysis of crystal structures ... roles of His121 and Val122 in catalysis and regulation of dCTP deaminase Removal of the imidazole ring in H121A was anticipated to relieve or reduce inhibition by dTTP by preventing expulsion of...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... swelling-induced inhibition of proteolysis in perfused rat liver J Nutr 126, 395–402 Haussinger D & Schliess F (1999) Osmotic induction of ¨ signaling cascades: role in regulation of cell function Biochem ... activation of the p38-type MAPK and thereby inhibition of 5800 autophagic proteolysis [14] Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin ... of 3T3 ﬁbroblasts to growth factors [17,18] Activation of Na+ ⁄ K+ ⁄ 2Cl– cotransport via NKCC1 was shown to be essential for cell cycle progression in 3T3 ﬁbroblasts [17,19] The importance of...

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Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx

Ngày tải lên : 18/02/2014, 16:20

... malabsorption of folate was observed over the entire course of ethanol treatment of months Determination of the kinetic constants of [3H]folic acid uptake in BBMVs V(pmol/30 sec/mg protein) The effect of ... primary absorptive site of folic acid, i.e the small intestine Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level of expression of the RFC protein ... expression of a major folate transporter, RFC, was studied The investigation of the regulation of folate transport via RFC expression in absorptive epithelia may aid in the development of future...

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