reconstruction macromolecular machines and structural proteomics
Module 2: Architecture and Structural Design Patterns
... creational and structural design patterns can be used to define an architectural template This section discusses creational and structural design patterns and their uses Module 2: Architecture and Structural ... Architecture and Structural Design Patterns The purpose of this lab is to help students become more familiar with structural design patterns and architecture templates Consider discussing the scenario and ... Architecture and Structural Design Patterns Structural Topic Objective To provide a background about structural design patterns Lead-in Recordset In this topic, you will learn about the structural...
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Regenerative Medicine Reconstruction of Tracheal and Pharyngeal Mucosal Defects in Head and Neck Surgery
... in use for the reconstruction of the mucosa of the urinary tract [162] and the cornea [163] and for teeth regeneration [164], the development of mucosal reconstruction in head and neck surgery ... Wendlandt, M., Neuenschwander, P., and Suter, U.W (2007) Surface-textured PEG-based hydrogels with adjustable elasticity: synthesis and characterization Biomaterials, 28, 567–575 Tang, Z.G and ... open new perspectives for the tracheal reconstruction in children Cartilage was harvested so far from ribs, nasal septum, and ears, and also from tracheal and joint cartilage While Kojima et al...
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Tài liệu Module 2: Architecture and Structural Design Patterns pdf
... creational and structural design patterns can be used to define an architectural template This section discusses creational and structural design patterns and their uses Module 2: Architecture and Structural ... Architecture and Structural Design Patterns The purpose of this lab is to help students become more familiar with structural design patterns and architecture templates Consider discussing the scenario and ... Architecture and Structural Design Patterns Structural Topic Objective To provide a background about structural design patterns Lead-in Recordset In this topic, you will learn about the structural...
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Tài liệu Notes for an Introductory Course On Electrical Machines and Drives E.G.Strangas MSU Electrical ppt
... Voltage and Frequency 7.4 Operation from a Source of Variable Frequency and Voltage 7.5 Controllers for PMAC Machines 7.6 Brushless DC Machines 81 81 81 82 82 Line Controlled Rectiers 8.1 1- and ... understanding of modern machines and drives Power electronics are introduced, at the device and systems level, and electrical drives are discussed Equations are kept to a minimum, and in the examples ... amplitudes and v , and initial phases, i and v , and the same frequency, = 2/T 2f : i v(t) i(t) = v sin(t + v ) = sin(t + i ) i (1.2) (1.3) In this case the rms values of the voltage and current...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... interactions: binding and cleavage by ribonuclease A and variants at Lys7, Arg10, and Lys66 Biochemistry 37, 1212112132 31 Anderson DG, Hammes GG & Walz FG Jr (1968) Binding of phosphate ligands to ribonuclease ... functional and structural studies presented herein demonstrate the attributes of N-acylsulfonamidyl and sulfonimidyl groups as surrogates for the phosphoryl groups of nucleic acids The structural ... RiIi(h), where Ii(h) and I(h) are the ith and the mean measurements of the intensity of reection h, respectively Rcryst = Rh |Fo ) Fc| RhFo, where Fo and Fc are the observed and calculated structure...
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Chapter 10 DC machines and drives
... steps low and S1 and S2 are opened and S3 and S4 are close The armature * current decreases until ia = ia − ε , at which time the comparator output becomes high; S1 and S2 are closed and S3 and S4 ... 10.11-2) When the output of this comparator (L) is high, S1 and S2 are closed and S3 and S4 are open; when L is low, S3 and S4 are closed and S1 and S2 are open The action of this type of current control ... state, which occurs over interval A, S1 and S2 are closed and S3 and S4 are open The second state occurs over interval B, wherein the S3 and S4 are closed and S1 and S2 are open As in the case of the...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... for structural studies of conotoxins and their analogs [24–27] Most NOESY crosspeaks were assigned and integrated, with concomitant cycles of structure calculations for evaluation of distance and ... better Ramachandran plots were chosen to represent the three-dimensional solution structure of conomarphin, and the mean structure was generated by molmol Structural characterization and comparison ... kinetics of the Trp14 and Thr15 amide protons The two observed small 3JHN-Ha coupling constants for residues Asn11 and Ser12, and the dNN(i,i + 2), daN(i,i + 2) [Phe13(CaH)–Thr15(NH)] and daN(i,i + 3)...
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Tài liệu Báo cáo khoa học: "Reading Level Assessment Using Support Vector Machines and Statistical Language Models" pdf
... e.g Lee and Myaeng’s (2002) genre and subject detection work and Boulis and Ostendorf’s (2005) work on feature selection for topic classification For our LM classifiers, we followed Boulis and Ostendorf’s ... development and test sets The dev and test sets are the same size and each consist of approximately 5% of the data for each grade level measures For comparison to other methods, e.g Flesch-Kincaid and ... results Figures and show DET curves for this set of classifiers on the development set and test set, respectively The grade and classifiers have the best performance, probably because grade and must be...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... surfaces was 1.4 A and a standard ˚ Stern layer was used to exclude ions from the 2.0 A molecular surface [37] The inner and outer dielectric constants applied to the protein and the solvent were, ... Concentrations of glucose, maltose and cyclohexaamylose were 80 mM , 80 mM and 1.25 mM , respectively The substrate concentration ranged between 0.025 and 0.5% (w/v) Fig SDS/PAGE and isoelectric focusing ... mass of 56 073 Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, respectively...
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Báo cáo khoa học: Cholesterol oxidase: biochemistry and structural features ppt
... made by the residues found between the third and fourth b-strands of a four-stranded b-pleated sheet (Fig 4B) Interaction between the phosphate oxygens and the protein involve main chain nitrogen ... elevated Km values for both cholesterol (Km = lm for SChOx and > 100 lm for ReChOx) and dehydroisoandosterone (Km = 27.5 lm for ChOx and 400 lm for ReChOx) in ReChOx [33] Indeed, the increased ... the specific roles of catalytic residues and the mechanisms of both oxidation and isomerization [54] Mutagenesis and kinetic analyses, as well as further structural studies, have helped to identify...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... expression vectors and USP7 cDNA as well as Shirley Gil-Parrado, Bruno Martoglio, Martin Renatus and Jorg Eder (Novartis, Basel, Switzerland) ¨ ´ for support and helpful discussions A Fernandez´ Montalvan ... ¼ 1.4 lm) [30] and USP2 (KM ¼ 0.554 lm) [25], respectively, and displays a similar KM as USP8 (KM ¼ 10.2 lm) [39] Differences in the ubiquitin recognition mechanisms and in the structural rearrangements...
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ENGINEERING SEISMOLOGY, GEOTECHNICAL AND STRUCTURAL EARTHQUAKE ENGINEERING pot
... effects, and seismo-induced landslides, tsunamis as well as and the design and analysis of structures to resist such actions The study of earthquakes ties together science, technology and expertise ... amplification of and Tanaka 1961 and Kanai 1962, Kagami et al, 1982 and 1986; Rogers et al., 1984; Lermo et al., 1988; Celebi et al 1987) 17 18 Engineering Seismology, Geotechnical and Structural Earthquake ... a Greek and a Turkish city: Seismic hazard, Geotechnical and Lifeline Aspects”, Earthquakes and Structures,Vol 2, No 3, September 2011 33 34 Engineering Seismology, Geotechnical and Structural...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic ... combination of CM-TSK and RP-HPLC The final material was homogenous, as shown by the presence of a single band on SDS ⁄ PAGE, a single sharp peak on RP-HPLC, and a single band on immunoblotting ... Chow et al isoforms and these have high pI values ranging from 9.5 to 10.5 [10–15] Similarly, Cyn d is a calcium-binding protein containing two Ca2+, and its allergenicity and cross-reactivity...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... at 1683, 1669 and 1663 cm)1 are possibly assigned to turns or bends; and the band at 1647 cm)1 is likely to be the random coil structure As occurred in FTIR, the Raman amide III¢ and I¢ regions ... of protein and one might expect the above solution structure to be reflected in Fig The 1652 and 1270 cm)1 bands can be assigned to a-helix in the human PKIb; the 1666 and 1247 cm)1 bands are the ... 1669, 1653, 1647 and 1636 cm)1, and two shoulders at 1663 and 1625 cm)1, which indicate that the amide I¢ mode consists of various overlapping components (Fig 4A) These component bands can be better...
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Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... abrin-a, abrin-c and ricin are composed of ve invariant residues (Tyr74, Tyr113, Glu164, Arg167 and Trp198 in abrin-a and abrin-c, and Tyr115, Tyr158, Glu212, Arg215 and Trp246 in ricin) and another ... A-chain: cloning and structural studies 1.5 and 72 C for min), 25 cycles (94 C for min, 60 C for 1.5 and 72 C for min) followed by 10 at 72 C to a nal extension Both amplied fragment and pGEX 5X-1 ... association of rPAC and rPBC Fig N-glycosidase activities of rPAC and native pulchellin Yeast ribosomes (20 lg) were incubated with different amounts (10, 5, and 0.1 ng) of rPAC and native pulchellin...
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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... by the local ethics committee and performed according to the standards set by the Declaration of Helsinki The experiments were undertaken with the understanding and written consent of all subjects ... 18S and 28S ribosomal RNA bands after electrophoresis The RNA was quantied by spectrophotometry at 260 nm and stored at )70 C One microgram of total RNA was used to synthesize cDNA using a standard...
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Báo cáo khoa học: Bistability from double phosphorylation in signal transduction Kinetic and structural requirements potx
... cycle (steps and 2) and cycle (steps and 4) (Eqn 7) and the values of a, c and b only depend on the KS value (Eqn 8) The concentrations of interconvertible forms are: c¼a¼ þ KS and b¼ À KS ð8Þ ... constants of the modifier and demodifier enzymes are equal, namely, KS1 ¼ KS3 and KS2 ¼ KS4, and considering Eqn (2) and Eqn (3), the following mathematical expressions for a, b and v14 can be expressed ... 10)2) and r31 ¼ r24 (A), (B) and (C) show the molar fractions a ([Wa] ⁄ WT), b ([Wb] ⁄ WT) and c ([Wc] ⁄ WT) as functions of v14, respectively (D) and (E) show the steady-state fluxes of cycles and...
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... DNA polymerase (Toyobo, Japan), using the bF and 68r primers, bR and 68f primers, aF and 109r primers, aR and 109f primers, aF and 114r primers, or aR and 114f primers Following an initial denaturation ... 55 °C, and a incubation at 68 °C The second PCR was also performed with KOD-plus DNA polymerase, using the two megaprimers and the bF and bR primers, or the two megaprimers and the aF and aR ... reaction, was used The 0.72 kb DNA fragment and pUC18-NHase were digested with EcoRI and NotI, and the 1.08 kb DNA fragment and pUC18-NHase were digested with NotI and HindIII The PCR product was ligated...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25