... concentrations of [125I]MIP-1a and cold MIP-1a or RANTES (1000 and 500-fold [125I]MIP-1a quantities) Unbound ligand was removed by filtration on BSA-presaturated GFB-filters (Whatman), and the filters were ... concentrations between 0.1 and 0.4 mM was added to liposomes (66 lM of lipid) containing N-NBD-PtdEth and N-Rh-PtdEth at time 0, and the fluorescence of N-NBD-PtdEth was measured and normalized to a ... ultracentrifugation The consecutive supernatants S1, S2 and S3 and the final proteoliposome pellet (PL) were analysed by Western blot and autoradiography, and show successful reconstitution of the protein,...
... Differential effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... transfected with RARa and ⁄ or RXRa (without RA treatment), simultaneous ligand activation of RARa and RXRa (cotransfection of RARa and RXRa together with treatment of both ATRA and 9-cisRA) provided ... TE671 JEG-3 ‡ ‡ ‡ No treatment Ligand-bound (RARαRXRα) dimer Fig Effects of ligand-activated RARa and RXRa on hGnRH II gene expression The effect of ligand-bound RARa and RXRa on the endogenous hGnRH...
... neighbors b-RAF and mixed-lineage kinase (MLK)3 require self-interaction and dimerization for proper regulation and activation [20,21] Similarly, LRRK2 forms structures consistent with dimers and oligomers ... government works P J Webber and A B West Function in cells andneurodegeneration domain death agonist (Bid) activation, leading to translocation to the mitochondria and possible release of cytochrome ... allows kinase activity, autophosphorylation and stabilization of a P J Webber and A B West kinase-active homodimer (Fig 1) Competing phosphatase activity and loss of GTP, as well as the subsequent...
... pathological roles in schizophrenia, ageing and acute and chronic neurodegeneration (see the text for references) healthy volunteers, and precipitate thought disorder and delusions in schizophrenia patients ... enzyme and b-elimination catalyzed by serine racemase Neutral amino acid transporters; Asc-1 in neurons and ASCT-like in astrocytes Vesicular and nonvesicular release modes described in neurons and ... Glycine and N-methyl-d-aspartate receptors: physiological significance and possible therapeutic applications Pharmacol Rev 50, 597–664 Paoletti P & Neyton J (2007) NMDA receptor subunits: function and...
... of Differentiated Endothelial Cells: Von Willebrand Factor Von Willebrand Factor (VWF) The Von Willebrand factor (VWF) binds to platelet receptorsand activates these cells [175] The VWF- precursor ... mechanisms of ligand binding, signaling, and regulation within the superfamily of G-protein-coupled receptors: molecular modeling and mutagenesis approaches to receptor structure and function Pharmacol ... facilitate a better understanding of numerous complex biological processes involved in angiogenesis Thus, PKC is listed in categories #1 and #4 and C3/C03 is listed in #1 and #9 Among a diverse family...
... ethylene receptors Model for signaling by ethylene receptors CTR1 (shown in gray) interacts with all five receptors, but subfamily receptors activate CTR1 to a greater extent than subfamily receptors ... subfamily receptors ETR2, ERS2, and EIN4 lack residues considered essential for histidine kinase activity and have instead been proposed to act as serine/threonine kinases [12] Some of the ethylene receptors ... ETR1 and ERS1, named etr1-9 and ers1-3 Our results indicate that both etr1-9 and ers1-3 are null alleles for their genes, whereas ers1-2 is a partial LOF allele Analysis of the etr1-7;ers1-3 and...
... (cysLTs) receptors related family, the lipoxin (ALX) receptorsand the prostanoid receptors class [1] Interaction of cysteinyl leukotrienes receptors (CysLT1 and CysLT2) with theirs ligand LTC4, ... prostaglandin D (DP) receptor, the prostaglandin E2 receptors (EP1, EP2, EP3 and EP4), the prostaglandin F receptor (FP), the prostaglandin I receptor (IP), the tromboxane A receptor (TP), and a ... However, in the CRS and EP1 and EP3 receptors were downexpressed compared to EP2 and EP4 and this difference was more accentuated in the CRS-NP subjects On the other hand CysLT1 and CysLT2 mRNA levels...
... contributions AMD; HK and YHA conceived of the study, participated in its design and collected the adrenal gland and uterine tissues (including weighing and judging reproductive status) SS and YHA performed ... were run in duplicates The inter- and intra-assay coefficients of variation were as follows: 20.0% and 42.5% at 3.2 pmol/l; 7.7% and 5.0% at 46.5 pmol/l; and 12.0% and 6.2% at 123.2 pmol/l The minimal ... epithelium, glandular epithelium and connective tissue stroma) and myometrium (see Table and Fig 1) For all mares, the highest proportion of ERα staining was, in general, found in the glandular epithelium...
... purification and Ca2+ binding ability study 25 4.2 BAR domain cloning, expression and purification 31 4.3 ΔBAR domain purification and Ca2+ binding ability study 36 4.4 SH3 domain expression and purification ... quantitative and qualitative studies of the structures and functions of the EEN and its three domains Native PAGE, Circular Dichroism and NMR were used to exploit the interaction of Ca2+ with EEN and ... understanding of the possible role of the random coil PRD and the influence of Ca2+ on EEN in vitro Chapter concludes the finding of this research and gives future perspectives CHAPTER Background and...
... Dissociation time courses and effects of unlabeled ligands and low pH on the dissociation rates of [125I]TC-PCSK9 (A) and [125I]TC-PCSK9-D374Y (B) from HepG2 cells Binding to equilibrium and removal of ... to dimeric receptors that release bound ligand slowly because they convert rapidly The slow phase of association could represent binding to monomeric receptors that release bound ligand rapidly ... internalize and degrade [125I]TC-PCSK9 at 37 °C However, the interaction of ligands with cell surface receptors at 37 °C is a function not only of the rate constants of association and dissociation,...
... included a DNase I treatment, and cDNA synthesized as described above The time points analyzed were 1–2 and 4–5 days pre-vitellogenesis, and 6, 12, 24, 36, 48 and 72 h PBM To standardize qPCR inputs, ... was extracted from the fat body and ovaries of pre-vitellogenic females 5–6 days after eclosion and from vitellogenic females 6–12 and 18–24 h after a blood meal, and then was subjected to 1236 ... An gambiae and Ap mellifera NRs, and one which is a likely ortholog of the Ap mellifera and T castaneum PNR-like NR [20–22] that is also present in the genome of An gambiae (Table S1 and Fig 1)...
... processes Secreted ADP binds to the P2Y1 and P2Y12 purinergic receptors, and triggers shape change, Ca2+ mobilization and platelet aggregation [13–15] The P2Y1 receptors are linked to Gq, but they ... the Ca2+ response relies on both phospholipase C and PI3-K activity PI3-Kb and not PI3-Kc mediates the P2Y12 effect on thrombin-evoked Ca2+ responses In man and mouse, the PI3-Kb (p110b) and ... ADP-evoked phospholipase C activity and Ca2+ mobilization in a way controlled by PI3-Kb Results Autocrine and added ADP increases and prolongs thrombin receptor-induced Ca2+ responses via P2Y12 receptor...
... ligand affinity between Y7 and Y2 may prove very useful for studies of ligand–receptor interactions and 3D modeling, and we have previously been able to utilize differences between chicken and ... distribution and phylogeny of the chicken Y6 and Y7 receptorsand performed the initial pharmacological characterization of the latter It is clear, from these studies, that the Y6 and Y7 receptors ... radioligand This radioligand had iodinated tyrosines at positions 21 and 27 and a specific activity of 4000 CiÆmmol)1 Saturation experiments were carried out with serial dilutions of radioligand, and...
... °C with a Ca2+ -sensitive electrode (World Precision Instruments, Inc.) using calibration standards in the range from 10)8 to 10)1 M free [Ca2+ ] Results The Ca2+ -sensor proteins GCAP-1 and GCAP-2 ... HEK293 cells and COS cells Cell membranes were reconstituted with D6SGCAP-1 and GCAP-2 and the EC50 and IC50 values were determined EC50 values were similar as described previously [25,44] and maximal ... between GCAP-1 and GCAP-2 in their regulatory properties and if so, what is the physiological significance of such a difference? The study demonstrates that GCAP1 and GCAP2 modulate the Ca2+ signaling...
... expression of HIV- and FLAG-tagged D2L and D2S receptors in Sf9 cells To achieve this, mAbs directed against gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern visualized ... Anti-gp120 Ig and anti-FLAG Ig identified bands corresponding to proteins with a molecular mass equivalent to 43 kDa and 85 kDa for D2L and 39 kDa and 80 kDa for D2S (Fig 1) No bands were detected ... the dopamine D2L and D2S receptors can form constitutive homo- and hetero-oligomers in two expression systems (Sf9 and HEK293 cells) and these are not regulated by receptor ligands Our study applied,...
... the resolution of the X-ray and NMR structures of the Wntx called bucandin and WTX [16,27,28] When we started this work, 22 amino acid sequences of Wntxs were known and it was clear to us that ... Considering that the two toxins display 11 residue differences and that the competition systems used (human and rat on one hand, and chicken on the other) are not identical in the two studies, ... precipitates were washed three times and dried, dissolved in 10% acetic acid and lyophilized The synthetic toxin was reduced with molar excess of TCEP under acidic conditions and purified by RP-HPLC using...
... lL HW-55S solution with Ca2+ and b-1,3-glucan (d), Ca2+ only (m), b-1,3-glucan only (j) and with neither Ca2+ nor b-1,3-glucan (s) than a combination of b-1,3-glucan and Ca2+ This result suggests ... Toyopearl CM-flow-through and Toyopearl CM-eluate were incubated in the presence of b-1,3-glucan and Ca2+ , PO activity was clearly shown to be dependent on b-1,3-glucan and Ca2+ (column 5) Tenebrio ... presence of b-1,3-glucan and Ca2+ (lane 11 in Fig 7B) The activation ratio of the 55-kDa zymogen form in the presence of b-1,3-glucan and Ca2+ was faster than in the presence of Ca2+ only (compare lane...
... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... and repeated three times in the presence of Ca2+ we lysed the yeast cells in the presence of Ca2+ (10 mM) or EGTA (1 mM) and examined PtdEtn-PLD activity in the 100 000 g pellet (membranes) and ... Spo14p/Pld1p is Ca2+ -independent and thus may be assayed in the presence of EGTA and EDTA, conditions under which PtdEtn-PLD is inactive On the other hand, PtdEtn-PLD hydrolyses PtdEtn and thus may...
... isolated EF-hand motif of STIM1 undergoes Ca2+ -induced conformational changes and remains as a dimer in the absence and presence of Ca2+ Results and Discussion The isolated EF-hand motif from ... EF-hand from STIM1 binds to Ca2+ Y Huang et al Fig Grafting the helix–loop–helix EF-hand motif into CD2 (A) The sequence alignment results of calmodulin EF-hand III and the canonical EF-hand ... EF-hand binds to Ca2+ and lanthanide ions One of the most important steps to fully understand the mechanism underlying the Ca2+ -modulated functions of STIM1 is to investigate the site-specific Ca2+ binding...
... to Ca2+ -binding sites is known to generate the J-band (610–650 nm) and ⁄ or the c-band (480–510 nm) Upon binding to Calnuc, the dye displayed prominent J-band and c-band in both absorption and ... b-crystallin and d-crystallin behave similarly (show only one band), whereas calmodulin and troponin behave like each other On the other hand, Calnuc and parvalbumin generate both the J-band and the c-band, ... the c-band, respectively; with parvalbumin, however, the J-band is retained at all stoichiometries Also, whereas the J-band is replaced by the b-band and the c-band upon the addition of Ca2+ to...