recent breakthrough studies on lysozyme

Some studies on a probabilistic framework for finding object-oriented information in unstructured data

Ngày tải lên : 23/11/2012, 15:04

... plausible solutions, which have been proposed recently with focus on the novel machine learning framework to solve the problem 2.1 Information Extraction Systems One of the first solutions in object ... Definition 2: An object query is defined by a conjunction of n attribute constraint Q = (c1 ^ c2 ^ … ^ cn) Some constraints would be constant when the user does not care about the attributes Each constraint ... regular expression, the features “where such regular expression existed” should be used - Constraint satisfaction features (CSF) Since the object queries contain constraints on each attribute...

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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

Ngày tải lên : 18/02/2014, 12:20

... electronic environment The nature and type of amino acids and their side-chain orientations around iron– sulfur cluster coordination sites are the likely cause of minor variations in the electronic ... solvent-induced conformational change, which is possibly because of changes in the chemical environment around the iron–sulfur cluster, the partial destruction of the cluster or its conversion to other ... order to acquire ﬁrm evidence for this observation, the total iron content of proteins puriﬁed under different conditions was measured The total iron content of the native and in-column refolded...

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Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

Ngày tải lên : 18/02/2014, 14:20

... scavenging and remobilization when the cells are under stress and consequently mononucleotide phosphate concentrations are high 5862 Materials and methods Cloning and puriﬁcation of St SurE The SurE ... R192 Stability of St SurE Urea denaturation studies were carried out by incubating the protein at a concentration of 0.5 mgÆmol)1 with varying concentrations of urea (0–7 m) for h after which CD ... melting studies were conducted using circular diachronic monitoring at both 209 and 222 nm The protein lost its secondary structure at a temperature of around 45 °C (Fig 4B) This was also conﬁrmed...

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Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

Ngày tải lên : 18/02/2014, 16:20

... conformations in E53QbsSHMT-Gly In one conformation, the carboxylate group forms hydrogen bonds with R357, as in bsSHMT-Gly [12] In the second conformation, the carboxylate forms hydrogen bonds ... present close to the position corresponding to the second conformation of the carboxylate group (Fig 2A,B) The hydrogen bonding between Q53 and the second conformation of carboxylate of Gly found ... illustrating electron density (omit map) corresponding to PLP and Gly in E53QbsSHMT-Gly The two conformations of Gly are shown A water molecule is present close to the second conformation of Gly with...

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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

Ngày tải lên : 20/02/2014, 01:20

... troponin-tropomyosin regulation of skeletal muscle contraction Annu Rev Biophys Biophys Chem 16, 535–559 Farah CS & Reinach FC (1995) The troponin complex and regulation of muscle contraction ... site to the inhibitory region) participates in the Ca2+-dependent activation In addition, at 15°C, similar activation is observed for the troponin containing the corresponding vertebrate TnI-fragment, ... molluskan muscles, the C-terminal region does not function and troponin regulates contraction only through the activation exerted by the N-terminal region of TnI Experimental procedures Muscle proteins...

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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Ngày tải lên : 22/02/2014, 07:20

... containing mM dithioerythritol The column was developed with the same buffer Fractions were combined and concentrated using a Centriprep-10 concentrator (Amicon) The ﬁnal protein concentration ... nonmevalonate pathway Tetrahedron Lett 41, 703 –706 Kuzuyama, T., Takagi, M., Kaneda, K., Watanabe, H., Dairi, T & Seto, H (2000) Studies on the nonmevalonate pathway: conversion of 4-(cytidine 50 -diphospho)-2-C-methyl-D ... by reversed phase ion pair HPLC monitored by scintillation counting HPLC analysis of phosphorylated metabolites Isolation of chromoplasts from N pseudonarcissus The isolation followed a procedure...

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Case Studies on the Effectiveness of State Financial Incentives for Renewable Energy pdf

Ngày tải lên : 06/03/2014, 19:20

... discourage installations Program participants tend to be strongly motivated by noneconomic factors Concerns about environmental issues, a desire to reduce dependence on utilities, and more recently, power ... quality-control provisions, requiring preapproved systems and installers in addition to post-installation monitoring This intensive component is part of the overall program mission to monitor system ... municipal utilities, nonprofit organizations and other nontaxable entities can serve as valuable technology-demonstration venues Finally, details on program participation, technology and capacity...

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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

Ngày tải lên : 07/03/2014, 03:20

... and the fractional contribution of the additional lifetime was close to zero Figure shows the typical time-dependent donor decays for the D1–CFP bearing donor alone and with the donor and acceptor ... Saturation studies were carried out on a fresh membrane preparation (ﬁnal protein concentration of 20 and 40 lgÆtube)1 for the D1 and D2 dopamine receptor, respectively) using concentrations of ... This conclusion is further justiﬁed by confocal microscopy analysis of receptor localization Analysis of the localization of dopamine D1, D2 and their genetic variant fusion proteins Confocal...

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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt

Ngày tải lên : 08/03/2014, 02:20

... application of this assay Under all conditions used, the reaction rate is directly proportional to the EcIPDC concentration and independent of the concentration of the auxiliary enzyme, conﬁrming ... pseudo ﬁrst-order rate constants of reconstitution calculated from these time courses show a hyperbolic dependence on the ThDP concentration (at saturating Mg2+ concentration), pointing to a two-step ... same conditions In the case of ZmPDC maximum enzyme concentration was 2.3 mgÆmL)1 Measurements with ScPDC were performed at an enzyme concentration of 90 lgÆmL)1 The plots of the reaction rate...

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STUDIES ON CATTLE MILK AND MEAT PRODUCTION IN FOGERA WOREDA: PRODUCTION SYSTEMS, CONSTRAINTS AND OPPORTUNITIES FOR DEVELOPMENT doc

Ngày tải lên : 08/03/2014, 23:20

... basis of per capita consumption in Sub-Saharan Africa, is estimated at 500 million kg Based on this calculation, there will be a minimum annual demand for one billion 14 million kg of milk to satisfy ... Insemination Center NGO =Non Governmetal Organization PA =Peasant Association PL =Pasture land RGDP =Regional Gross Domestic Product SAERP =Sustainable Agricultural and Environmental Rehabilitation ... 1998a the per capita consumption of milk is estimated at 19 liters per year, while meat consumption is about 13.9 kg a year Accordingly, about 495 thousand tones and million tones of milk is required...

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oecd studies on environmental innovation better policies to support doc

Ngày tải lên : 09/03/2014, 12:20

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Studies on Business Administration and Management and ethical and social values docx

Ngày tải lên : 16/03/2014, 03:20

... Pearson’s correlation coefficient Pearson’s correlation coefficient Pearson’s correlation coefficient Pearson’s correlation coefficient Pearson’s correlation coefficient Pearson’s correlation coefficient ... V2 Pearson’s correlation coefficient Sig (bilateral) V2 Pearson’s correlation coefficient Pearson’s correlation coefficient Pearson’s correlation coefficient * 216 Pearson’s correlation coefficient ... Education Area: responding to challenges in a globalised world, London Communiqué, 18 May 2007, from http://www.dfes.gov.uk/londonbologna/uploads/documents/LondonCommuniquefinalwithLondonlogo.pdf,...

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Báo cáo khoa học: Biosynthesis of isoprenoids – studies on the mechanism of 2C-methyl-D-erythritol-4-phosphate synthase pdf

Ngày tải lên : 16/03/2014, 06:20

... above, these initial conditions were rapidly conducive to steady conditions where and were present in very similar concentrations, and the rates of the forward reaction (conversion of to 3) and the ... 4-phosphate (3c) Table S6 Conversion ratios and cycles for the IspC reaction containing hydroxyacetone Table S7 Calculated hypothetical cycles for the IspC reaction containing [1,2-13C2]glycolaldehyde ... (near-stoichiometric) concentrations Under these conditions, the substrate molecules should be engaged by enzyme molecules on a nearpermanent basis The residual enzyme activity after 24 h of incubation was measured...

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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

Ngày tải lên : 16/03/2014, 10:20

... denaturation ACP at 60 lm was used in all the uorescence studies Fluorescence spectra were recorded on a Jobin-Yvon Horiba uorometer (Kyoto, Japan) under computer control The excitation and emission monochromator ... transition (Fig 2C) Unfolding transitions were also monitored by following the tyrosine uorescence at 305 nm upon excitation at 280 nm This was done because PfACP is devoid of tryptophan but contains ... sedimentation analysis Forty micrograms of apo-ACP and holo-ACP were layered on top of a mL continuous 010% (w v) sucrose density gradient, and this was followed by centrifugation, fractionation and...

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Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

Ngày tải lên : 16/03/2014, 18:20

... addition, our experiments demonstrated, for the ﬁrst time, that mutations at other conserved positions of motif I also affect enzyme functioning (Fig 1) (Table 2) In fact, substitution at position ... severe effect However, while nonconservative substitutions (M53N) resulted in a strong decrease in catalytic efﬁciency, conservative substitution (M53I) led to effects on both enzyme activity and ... a conservative substitution (mutant T249S) yielded a kinetic behaviour more similar to that of wild-type enzyme In addition, the effect on enzyme activity exerted by a nonconservative mutation...

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Báo cáo khoa học: Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase Stability studies on the product-activated enzyme potx

Ngày tải lên : 16/03/2014, 18:20

... expression was induced by the addition of IPTG to a concentration of mm Protein translation was arrested by the addition of chloramphenicol to a concentration of 300 lgÆmL)1 after h of induction The ... is negative, the activation of PfHGPRT is entropically unfavorable Discussion The observations described above suggest that the conformation of PfHGPRT on puriﬁcation is one of high FEBS Journal ... Oligomerization is also a necessary, but insufﬁcient, condition for activation, with activation being most stable under conditions in which the enzyme is a tetramer PfHGPRT is a tetramer in low-ionic-strength...

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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

Ngày tải lên : 17/03/2014, 09:20

... cofactor and dopamine on the rate of phosphorylation (Fig 3), on limited proteolysis (Fig 1A) and on the global conformational transition (hysteresis) related to catalytic activation ể FEBS 2003 988 ... effects (a) on the limited proteolysis by trypsin, (b) on the reversible conformational transition (hysteresis) triggered by substrate binding, as followed in real time by surface plasmon resonance ... software from Nonlinear Dynamics Ltd, 1996 [10] Effect of H4biopterin/H2biopterin on the conformational transition (hysteresis) triggered by substrate binding as studied by surface plasmon resonance...

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REPORT ON STUDIES ON BENCHMARKING /INPUT NORMS FOR PULP AND PAPER INDUSTRY SUBMITTED TO CESS GRANTS AUTHORITY pot

Ngày tải lên : 18/03/2014, 02:20

... (dyes & pigments) Retention aids Drainage aids Optical brightner Applications Controls pH Controls pH, fix additives onto fibres, improve retention Control penetration of liquids Improve burst ... required for low consistency operation Water is now considered as one of the valuable resource, and mills are forced to reduce their water consumption levels Charter on corporate responsibility (CREP) ... areas, energy for pumping etc., Besides environmental effect, reduced energy consumption is associated with reduction in water consumption Energy consumption is low for a closed cycle plant, when...

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studies on tin oxide-intercalated polyaniline nanocomposite for ammonia gas

Ngày tải lên : 20/03/2014, 13:08

... formation of initial seeding on the fresh substrate In the reaction bath without a substrate into it, aniline monomers are in random thermo-dynamic state, and depending upon thermo-chemical conditions ... aggregation These mechanisms depend on the dispersion of colloidal particles at lowsolid volume fractions (ϕ0 → 0) having with (a) diffusion-limited cluster aggregation (DLCA), and (b) reaction-limited ... aggregation (RLCA) In DLCA every collision between two clusters results in the formation of a new cluster, the aggregate of the two colliding clusters In RLCA only a small fraction of all the collisions...

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Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants ppt

Ngày tải lên : 22/03/2014, 17:20

... BS-RNase as a positive control For a negative control, the proteins were also assayed on nontumor 3T3 cell cultures at a ﬁnal concentration of 100 lgÆmL)1, and found to be nontoxic (Fig S2) The ... function of urea concentration (insets in Fig 5), whereas the curves of NCDs displayed two transitions (Fig 5) The values of urea concentration at half-completion of transition, C½, are shown ... concentrations, but focusing on the parent BS-RNase and on the single-point R80S variant The results are reported in Fig 7, where the folded fraction is reported as a function of the urea concentration...

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