real time tissue elastography and transient elastography for evaluation of hepatic fibrosis
báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... analysis was performed using forward and reverses primer (each at 25 μM) for IFNγ or CD8 The probes were used at 0.2 and 0.3 μL for IFNγ and CD8, respectively Positive control cDNA (IFNγ and CD8 plasmid, ... and earlier post vaccine time points However, there is no statistically significant linear trend between time (days) and the IFNγ response with a p-value > 0.05 (0.3506 for Real Time RT-PCR and ... assay, IFNγ real- time RTPCR, and INFγ ELISPOT met validation criteria Validation approaches provide a guide for others in the field to validate these and other similar assays for assessment of patient...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... denaturation step of 94°C for followed by 45 cycles of 94°C for s, 52°C for 10 s, 72°C for 17 s and a fluorescent read step of 84°C for 10 s to melt primer dimers For the creation of standard curves, ... molecules of plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig ... denaturation step of 94°C for followed by 40 cycles at 94°C for 10 s, 45°C for 10 s 72°C for 15 s and a fluorescent read step of 85°C for 15 s to melt primer dimers The reaction was performed using...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC ... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real- time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve dilutions ... end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad iCycler iQ™ Multicolor Real- Time PCR Optical System Software Version 3.1 A standard curve for the quantitation of...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... denaturation step of 94°C for followed by 45 cycles of 94°C for s, 52°C for 10 s, 72°C for 17 s and a fluorescent read step of 84°C for 10 s to melt primer dimers For the creation of standard curves, ... molecules of plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig ... denaturation step of 94°C for followed by 40 cycles at 94°C for 10 s, 45°C for 10 s 72°C for 15 s and a fluorescent read step of 85°C for 15 s to melt primer dimers The reaction was performed using...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... Analysis of HA and quantitative real- time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real- time PCR data employed for the determination of JC ... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real- time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve dilutions ... end of the cycle Real- time PCR amplification data were analyzed using the Bio-Rad iCycler iQ™ Multicolor Real- Time PCR Optical System Software Version 3.1 A standard curve for the quantitation of...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... Figure Binding studies for DAF and CAR Binding studies for DAF and CAR A) MOI 0.5 TCID50/cell ofCVB2O, EV7W and CVB5 was incubated with 2.5 × 105 cells in suspension for h at 4°C and virus attachment ... RNA extraction and realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive ... Analysis Of The Erythrocyte Receptor For Hemagglutinating Enteroviruses Virology 1964, 22:580-590 Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapid real- time RT-PCR assay for detection of...
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real-time systems design and analysis, 4th edition
... represent soft real- time systems Table 1.1 gives an illustrative sampling of hard, firm, and soft real- time systems There is a great deal of latitude for interpretation of hard, firm, and soft real- time ... there is the issue of the appropriate writing of the term real- time. ” Across technical and pedestrian literature, various forms of the term, such as real time, real- time, and realtime may appear ... Tools for the Practitioner Hardware for Real- Time Systems Real- Time Operating Systems Fundamentals of Real- Time Systems Programming Languages for Real- Time Systems + Software Design Performance...
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real-time systems design and analysis an engineers handbook
... EXAMPLE REAL- TIME SYSTEMS 13 Specification and design of real- time systems and correct representation of temporal behavior Understanding the nuances of the programming language(s) and the realtime ... engineering of real- time systems 1.3 EXAMPLE REAL- TIME SYSTEMS Embedded real- time systems are so pervasive that they are even found in household appliances and toys A small sampling of real- time domains ... commands within a reasonable amount of time (e.g., second), or it will become torturous to use Most of the literature refers to such systems as soft real- time systems Definition: A soft real- time...
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Báo cáo hóa học: " Use of Time-Frequency Analysis and Neural Networks for Mode Identification in a Wireless Software-Defined Radio Approach" pptx
... quantization, and sampling frequency, as required in SR applications [5] On the other hand, from a software point of view, the design of flexible procedures able to satisfy the constraints of a real- time ... was considered from a scalable and complete SR point of view, the number of standards should have been the largest; therefore, the necessary time and resources to perform a serial or parallel reception ... transforms of the two standards: (a) Bluetooth, (b) IEEE 802.11b Frequency Frequency Time Time (a) (b) Figure 3: (a) Wigner distribution and (b) Choi-Williams distribution of an FH signal 4.1 Time- frequency...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 1 potx
... EXAMPLE REAL- TIME SYSTEMS 13 Specification and design of real- time systems and correct representation of temporal behavior Understanding the nuances of the programming language(s) and the realtime ... engineering of real- time systems 1.3 EXAMPLE REAL- TIME SYSTEMS Embedded real- time systems are so pervasive that they are even found in household appliances and toys A small sampling of real- time domains ... commands within a reasonable amount of time (e.g., second), or it will become torturous to use Most of the literature refers to such systems as soft real- time systems Definition: A soft real- time...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 2 pptx
... subtraction has subtract and and subtrahend, multiplication has multiplicand and multiplier, and division has dividend and divisor In a generic sense, the two terms “operandam” and “operandum” can be used ... Memory access times can have a profound effect on real- time performance and should influence the choice of instruction modes used, both when coding in assembly language and in the use of high-order ... resultant and operandum as the same The 2-address instruction is of the form: op-code operandam, operandum As a mathematical function, the 2-address would be expressed as: operandum = op-code(operandam,...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 3 docx
... or Arrival Time ri , j The release time of the j th instance of task τi Phase φi The release time of the first instant of task τi Response Time Time span between the task activation and its completion ... being executed and, that some kind of failure is indicated This is a form of software watchdog timer Certain types of low-priority self-testing can also be performed in the background For example, ... arrays, and so on In addition, it is necessary to perform any self-diagnostic tests before enabling any interrupts Finally, real- time processing can begin 3.1 REAL- TIME KERNELS 85 3.1.4.4 Real- Time...
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real time systems design and analysis phần 4 ppt
... sigevent *event, timer_t *timer_id); As per POSIX standard, different platforms can have multiple time bases, but every platform must support at least the CLOCK_REALTIME time base A timer based upon ... UML-compliant series of modeling notations In any case, UML largely enjoys the benefits of both informal and formal techniques and is widely used in real- time systems specifications and design 4.4 FORMAL METHODS ... Limitations of Formal Methods Formal methods have two limitations that are of special interest to real- time system developers First, although formalism is often used in pursuit of absolute correctness and...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 5 doc
... Event All Traffic Standards/Display of Commanded Phase All Traffic Standards/Display of Commanded Phase All Traffic Standards/Display of Phase All Traffic Standards/Display of commanded phase Intersection ... responsible for managing the following functions: Instantiation of contained objects Control of the traffic standards associated with the approach Handling of pedestrian crossing events Handling of loop ... Interface/Initiation of Alarm Transmission All Traffic Standards/Display of Default State Intersection Controller/Cycle Time and Splits updated Intersection Controller/Cycle Time and Splits updated All Traffic Standards/Display...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 6 docx
... question of whether object-oriented design is more suitable then SD for the embedded real- time systems and the inertial measure unit in particular SD and object-oriented design are often compared and ... much in the way of stimuli and response Real- time systems are usually data intensive and would seem well suited to structured analysis But real- time systems also include control information, which ... m_PedestrianDetector[1] :OnOffSensor m_PedestrianDetector[0] :OnOffSensor m_PedestrianTrafficStandard[1] :IntersectionStandard Same messages as between m_PedestrianTrafficStandard[0] and m_Approach[0]...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 7 potx
... accuracy of these timers is machine dependent, and not guaranteed, reducing their usefulness for real- time systems C# and the NET platform may not be appropriate for hard real- time systems for several ... execution time and that lend themselves to performance prediction The compile time prediction of execution time performance is known as a schedulability analysis In the design of modern real- time ... precondition of BoundedBuffer, and hence violated substitutability (and as such is a questionable use of inheritance) Most opponents of object-oriented languages for real- time assert that concurrency and...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 8 doc
... time1 =current_clock _time( ); loop _time= time1 -time0 ; time2 =current_clock _time( ); /* read time now */ /* run empty loop */ for (i=1;i
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 9 doc
... patterns, and several different areas of memory Finally, normal checking of other system hardware is performed and standard initialization of the interrupt cycle structure and variables is performed Foreground ... test, for example, is to generate a configurable number of threads in a test program and subject the software to them Running such tests for long periods of time also has the benefit of checking for ... In short, for a given piece of software, the time, T , to complete is a function of L, the number of lines of delivered source instructions (KDSI), and two additional parameters, a and b, which...
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REAL-TIME SYSTEMS DESIGN AND ANALYSIS phần 10 potx
... “Evolution and State -of- the-Art of Real- Time Languages.” Journal of Systems and Software, Volume 18, Number 4, April 1992, pp 61–84 [Stoyenko86] Stoyenko, A D., and Kligeman, E Real- Time Euclid: ... alternation of the contents of memory soft real- time system A real- time system in which failure to meet deadlines results in performance degradation but not necessarily failure software A collection of ... P., Bihri, T., and Gupta, R “Compiler Support for Object-Oriented Real- Time Software.” IEEE Software, September 1993, pp 42–49 [Grehan98] Grehan, R., Moote, R., and Cyliax, I Real- Time Programming:...
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Báo cáo y học: "The effect of real-time CPR feedback and post event debriefing on patient and processes focused outcomes: A cohort study: trial protocol" ppsx
... England NHS Foundation Trust and University of Warwick Teresa Melody, Heart of England NHS Foundation Trust Page 12 of 17 Naheed Akhtar, Heart of England NHS Foundation Trust and University of ... UK legislation and the Standard Operating Procedures of the Department of Anaesthesia, Critical Care, Pain and Resuscitation The sponsor organisation for the trial is Heart of England NHS Foundation ... discharge and cerebral performance category of patients Page of 17 at discharge The process focussed outcomes focus on both quality of CPR and team factors Quality of CPR outcomes are: quality of 2222...
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