real time pcr for the detection of pathogens in meat
Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... previous real- time PCR assays [8,27] These advantages improved the multiplex real- time PCR assay in terms of shortening turnaround time for bacterial detection and reducing the risk of crosscontamination ... curves of the multiplex real- time PCR for Salmonella (S.) Enteritidis The results of the multiplex real- time PCR were determined using decimal dilution of S Enteritidis ATCC 13076 DNA The PCR reaction ... curves for the multiplex real- time PCR for Salmonella (S.) Typhimurium The results of the multiplex real- time PCR were determined using decimal dilution of S Typhimurium ATCC 14028 DNA The PCR reaction...
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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx
... test the null hypothesis of marginal symmetry, namely that the probability of an observation being classified into cell [i,j] in the contingency table is the same as the probability of being classified ... compares the numbers of Salmonella positive flocks in routine samples and in 5sock-samples for the remaining 26 broiler flocks In 16 flocks, where Salmonella was detected in the routine samples (ranging ... amount of Salmonella being excreted by chickens It is difficult to estimate the importance of these factors in this study because we know neither the time of infection nor the infective dose, the...
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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... ice all the time The master mix was divided with 50 µl going into each of the PCR tubes One or µl of DNA was added PCR was performed in the PCR machine (Gene Amp PCR system 9700) with an initial ... microscope Stretching of these metacercariae was done by hot formalin for two minutes Then, they were kept in Eppendorf tubes with neutral formalin (4%) Diagnosis of parasitic infections * Diagnosis ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the...
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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields" pps
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Báo cáo khoa học:" Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1?" pdf
... since the detection of the PCR products occurs online during real- time PCR amplification, hence greatly reducing the risk of cross-contamination and false positive results The optimization of ... viral DNA amounts, eliminating the need for sample dilution and minimizing sample handling The results for intra- and inter-assay precision indicate that both intra-assay and inter-assay CVs were ... developed ordinary TaqMan real- time PCR method Results Development and optimization of FQ -PCR and conventional PCR Following the optimization of FQ -PCR, final concentrations of primers each of 0.3...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" ppt
... Bruynseels P, Mertens AH: Evaluation of a rapid real- time RT -PCR assay for the detection of enterovirus RNA in cerebrospinal fluid specimens Journal of Clinical Virology 2002, 25:S39-S43 Noordhoek ... performed on the detection of Coxsackievirus A24 MP carried out the laboratory experiments, interpreted the results and wrote the manuscript WV defined the study, designed the primers, analyzed the data, ... maintain primer-probe set in our optimized assay but in our opinion analysis with primer-probe set can be useful in the case of equivocal results Further investigation however should be performed...
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Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf
... since the detection of the PCR products occurs online during real- time PCR amplification, hence greatly reducing the risk of cross-contamination and false positive results The optimization of ... viral DNA amounts, eliminating the need for sample dilution and minimizing sample handling The results for intra- and inter-assay precision indicate that both intra-assay and inter-assay CVs were ... developed ordinary TaqMan real- time PCR method Results Development and optimization of FQ -PCR and conventional PCR Following the optimization of FQ -PCR, final concentrations of primers each of 0.3...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc
... Bruynseels P, Mertens AH: Evaluation of a rapid real- time RT -PCR assay for the detection of enterovirus RNA in cerebrospinal fluid specimens Journal of Clinical Virology 2002, 25:S39-S43 Noordhoek ... performed on the detection of Coxsackievirus A24 MP carried out the laboratory experiments, interpreted the results and wrote the manuscript WV defined the study, designed the primers, analyzed the data, ... maintain primer-probe set in our optimized assay but in our opinion analysis with primer-probe set can be useful in the case of equivocal results Further investigation however should be performed...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses Table 1: PCR primers Primer1 RV2 QPCR Assay (Figure 2) RV2a RV2b RV2-FAM3 OSM QPCR ... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... Identification of a novel RV2 rhadinovirus in Macaca fascicularis using the RV2 QPCR assay Since the RV2 QPCR assay was based on consensus sequences shared by two distinct members of the RV2 lineage...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses Table 1: PCR primers Primer1 RV2 QPCR Assay (Figure 2) RV2a RV2b RV2-FAM3 OSM QPCR ... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... Identification of a novel RV2 rhadinovirus in Macaca fascicularis using the RV2 QPCR assay Since the RV2 QPCR assay was based on consensus sequences shared by two distinct members of the RV2 lineage...
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Báo cáo y học: " Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA" pot
... cycle of initial denaturation (95°C for 10 min) followed by 40 cycles of amplification at 95°C for sec, 55°C for 10 sec, and at 72°C for sec The final reaction volume was 60 μl containing 30 μl of ... Sensitivity of the ultra sensitive RTQ -PCR The sensitivity together with the 95% and 50% HBVDNA detection limits of the assay were estimated by using the WHO st International Standard for HBVDNA ... compared to the line of equality by testing the two-tailed hypothesis of slope = and the intercept = Results The ultra sensitive RTQ -PCR was performed in a LightCycler® 2.0 apparatus using a plasmid...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... assay Intrinsic performance of the duplex real- time RT -PCR assay Linearity Linearity of the duplex real- time RT -PCR assay was determined using serial 10-fold dilutions of a clinical sample at the ... Figure Linearity of the duplex real- time RT -PCR assay Linearity of the duplex real- time RT -PCR assay was determined using serial 10fold dilutions of a clinical sample at the following concentrations: ... correct The LOD of the duplex real- time RT -PCR assay was 38.99 IU/ml and the specificity was 100% Furthermore, the cost of the duplex real- time RT -PCR assay was considerably lower than that of the...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... scope of other diagnostic methods The real- time PCR assay permits the simultaneous detection and quantification of DNA It is useful for understanding the pathogenesis of the disease and the Previous ... PCR and FQ -PCR protocol The detection limit of the conventional PCR was determined based on the Fifty goslings were randomly divided into groups In brief, a group of 40 goslings were orally infected ... resulted in the presence of clear and distinct amplified fragments (441 bp) on the agarose gel The detection limit of the FQ -PCR was determined based on the highest dilution that resulted in the presence...
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Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
... unable to detect the infection during the early phase of the disease Thus there is a need for rapid and sensitive methods for detection of DENV early in the course of infection for better patient ... used for determining the specificity of the assay None of the 10 samples tested by the qRT -PCR assay showed positive amplification, suggesting that the test was 100% specific for the detection of ... Pradesh, India Methods Viruses Sixteen strains of dengue viruses, including five strains of DENV-1, four strains of DENV-2, three strains of DENV-3, four strains of DENV-4 and one strain each of JE,...
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Báo cáo hóa học: "Research Article Statistical Real-time Model for Performance Prediction of Ship Detection from Microsatellite Electro-Optical Imagers" docx
... presence of wind However, they remain active for a long time after the wind has blown), (4) choppy waves appearing for high wind speed and introducing nonlinearities in the sea surface model (they ... Finding a model for pm,bl (Rbl ) implies to include the effect of the PSF We can m either compute the convolution of the PSF with the image pixels or perform the FT of the image and multiply the ... the image Consider the application of a line -detection algorithm to the center pixel of the ship There are an in nite number of lines passing through this pixel These lines generate a curve in...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... relates to the index of the intensity of HPV infection This ratio of any specimen represents empirically a relative measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) ... pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio ... cleavage of the chemiluminescent substrate The emitted light was measured as relative light unit (RLU) in a luminometer (DML 2000, Digene®) The intensity of the light was proportional to the amount of...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... order, with the aim of obtaining an optimal list of genes for each Petunia line The results of the merged data revealed that the most adequate of the genes tested for normalization in Mitchell ... performed in partial fulfilment of the PhD degree of IM in the framework of the MSc-PhD program with Quality mention from the Spanish Ministry of Education MCD-2005-00339 Work performed in the ... for normalization was the same for both lines: using the proposed cut-off of 0.15 and comparing single developmental stages, the required number was two for Mitchell and V30 The requirement for...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
... designed and RT -PCR procedures were developed for the specific detection of PVM in various potato samples and for the confirmation of PCR amplicons The efficacy of RT -PCR for indexing seed potato ... determine specificity None of them yielded any amplification products To evaluate the sensitivity of the RT -PCR for the detection of PVM in dormant tubers using primers PVM3/PVM4, sap from PVM infected ... Discussion In this study, PVM was detected in several potato samples from an experimental farm in the province of Page of Figure Sensitivity of RT -PCR using primer set PVM3/PVM4 for detecting PVM in...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... stenosis from the individual centers included in the meta-analysis The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile ... measured by the MCG device The resulting mathematically integrated patterns of the abnormal indexes are then compared for their degree of abnormality to the abnormal index patterns in the reference ... according to whether the patients had prior revascularization The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile...
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