reactivation of latent herpes viruses in astronauts
Báo cáo hóa học: " Prevalence of Influenza A viruses in wild migratory birds in Alaska: Patterns of variation in detection at a crossroads of intercontinental flyways" potx
... prevalence of AI in waterfowl is typically low on wintering areas and declines further in spring, with prevalence rates in ducks of 0.4% in late winter and 0.25% as they return to nesting areas in Canada ... Alaska and our findings of AI virus prevalence by species, location, age, sex, and date We discuss our findings of lowpathogenic AI viruses in the context of sources of variation in prevalence ... prevalence of 25.6% but only sampled species of dabbling ducks, in August, at one location in interior Alaska A recent study in Canada found AI positives in 37% of 17 species of ducks (90% of which...
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Báo cáo y học: "Measles Resurgence Associated with Continued Circulation of Genotype H1 Viruses in China, 2005" pps
... Incidence and cases of measles cases in 31 provinces of China, 2004-2005 Incidence and cases of measles in 31 provinces of China, 2004-2005 1000 National Zhejiang Beijing Tianjin Shanghai Incidence / ... Hebei provinces assigned to Cluster2, the remaining strains were members of Cluster1 Cluster1 represented the predominant lineage of endemic measles viruses in the measles outbreak in China in 2005 ... sequences of the nucleotides coding for the carboxy terminal 150 amino acids of the N protein to the sequences of the WHO reference strains[6,9,10] (Figure 4) The clustering of MeVs in China within...
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Báo cáo thú y: " Prevalence and subtypes of Influenza A Viruses in Wild Waterfowl in Norway 2006-2007" ppsx
... prevalence of infection was found in dabbling ducks (189/ 1480, 12.8%), whereas lower prevalence were seen in diving ducks (2/49, 4.1%) and gulls (74/1213, 6.1%) The finding of a higher prevalence in ... Netherlands) according to the manufacturer's instruction, and eluted in 55 μl Detection of influenza A virus was performed using primers and probe targeting part of the 5'-end of the Matrix gene ... be transmitted to dabbling ducks feeding there, as compared to diving ducks feeding at deeper water levels It has been shown that influenza viruses can remain infectious in the surface water for...
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Báo cáo y học: "Taxonomic distribution of large DNA viruses in the sea" pptx
... fragments in this study We parsed the GOS PolB fragments to find intein insertions by the TIGRFAM profiles TIGR01445 (intein amino terminus) and TIGR01443 (intein carboxyl terminus) [65], but none of ... detectable intein domain In this study, we did not include the protein priming subfamily of the B family DNA polymerase [28], which is represented by the Pfam profile PF03175 The members of this ... (composed of 199 branches including internal ones) was also reduced by pruning branches leading to the non-selected leaves using BAOBAB [75] The reduced reference tree has 99 branches (including internal...
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Báo cáo sinh học: " Evidence that spontaneous reactivation of herpes virus does not occur in mice" pdf
... in TBS containing 1% bovine serum albumin Following incubation for hour, the slides were washed in three washes of TBS for minutes each and then incubated in the substrate consisting of diaminobenzidine ... seen (original magnification 400×) that ganglia containing infectious virus, either in the acute stage of infection or following reactivation, placed into the ear pocket resulted in spread of the ... of viral pathogenesis within 12 days of receiving ganglion transplants (Table 3) Five of 10 BALB/c scid mice receiving ganglion grafts containing 10 PFU and of 10 mice receiving grafts containing...
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báo cáo hóa học:" Evidence that spontaneous reactivation of herpes virus does not occur in mice" docx
... in TBS containing 1% bovine serum albumin Following incubation for hour, the slides were washed in three washes of TBS for minutes each and then incubated in the substrate consisting of diaminobenzidine ... seen (original magnification 400×) that ganglia containing infectious virus, either in the acute stage of infection or following reactivation, placed into the ear pocket resulted in spread of the ... of viral pathogenesis within 12 days of receiving ganglion transplants (Table 3) Five of 10 BALB/c scid mice receiving ganglion grafts containing 10 PFU and of 10 mice receiving grafts containing...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... reliable reference gene in HEP2 cells infected with the same strain of HSV-1) These findings therefore highlight the importance of re-evaluating the choice housekeeping genes when making even slight ... seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though the expression pattern of reference genes in HELA and HEP2 cells infected ... variation of 451 housekeeping genes spotted on microarrays in EBV infection in B cells from patients with Burkitt's lymphoma It is of note however that the γ -herpes virus EBV was not included in our...
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Báo cáo sinh học: " Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide" pptx
... recombinant nectin-1 was shown to block entry of virus through vaginal mucosa [21], suggesting the importance of nectin-1 in mediating entry of HSV into the female genital tract Hve-A and nectin bind ... re-amplification of hybridoma cDNAs, elimination of degenerate primer sequences introduced in the first reaction and introduction of 2/3 of a 15 amino acid hinge region comprising three repeats of four glycine ... addition of Larginine and oxidized glutathione (GSSG) in final two steps [28] The ability of the single chain antibodies produced in this way to bind their target antigen was tested by determining...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... secretion of IFN-γ at sites of HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans ... behavior of HSV-1 in NOD scid mice as the basis for concluding that NK cells play no role in innate resistance to HSV-1 Likewise, we question the validity of comparisons of HSV-1 infection in mice ... replication of herpesviruses [59-62] In the case of HSV-1, IFN-γ achieves this effect by multiplying the potency with which IFN-α/β inhibits viral replication [28] The uncontrolled spread of HSV-1 in...
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báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells" pot
... the onset of such damaging neuroinflammation have not been defined The rapid onset of inflammation following CNS infection suggests that resident glial cells play a pivotal role in the initiation ... Expression of a non-specific protein band observed following Coomassie blue staining is shown as a loading control (lc) for each blot For comparison purposes, DAI expression in murine small intestine ... presence of DAI, receptorinteracting protein (RIP3), stimulator of interferon genes (STING), and the HSV-1 gene product glycoprotein G-1 (gG1) in microglia, astrocytes, and whole brain samples were performed...
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báo cáo hóa học: " Prevalence of latent tuberculosis infection among health care workers in a hospital for pulmonary diseases" pptx
... >2 in relative risk [27] The infection risk of physicians was examined in various studies of varying quality Because of the inadequate data, it is difficult to evaluate the tuberculosis risk in ... reduce the indication of preventive chemotherapy The use of IGRAs in serial testing of health care workers is still not very well studied and the influence of the role of potential dynamic of IGRA ... course of a contact tracing or in serial testing of TB high- Page of (page number not for citation purposes) Journal of Occupational Medicine and Toxicology 2009, 4:1 risk groups following German...
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báo cáo hóa học: " Results of five-year systematic screening for latent tuberculosis infection in healthcare workers in Portugal" docx
... treatment of LTBI as a way of reducing the number of infected individuals, and the risk of progression to active TB [18] In our study, the distribution of TB cases was not uniform over the years in ... active TB in HCWs Methods The risk of M tuberculosis infection was assessed in 5,524 HCWs working or training in our hospital between September 2005 and June 2009 (Figure 1) According to the ... IGRA TST in mm Technician All (%) Total N Administrative Assistent 714 (61.3) 924 (53.9) population declined in the following years: 13 in 2006, 14 in 2007, in 2008 and HCW with active TB in the...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... reliable reference gene in HEP2 cells infected with the same strain of HSV-1) These findings therefore highlight the importance of re-evaluating the choice housekeeping genes when making even slight ... seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though the expression pattern of reference genes in HELA and HEP2 cells infected ... variation of 451 housekeeping genes spotted on microarrays in EBV infection in B cells from patients with Burkitt's lymphoma It is of note however that the γ -herpes virus EBV was not included in our...
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Báo cáo hóa học: " Genetic characterization of Measles Viruses in China, 2004" pptx
... the nucleoprotein (N) gene, of 59 measles isolates in this study, together with the WHO reference strains were shown in Fig The clustering of measles viruses in China 2004 within the genotype ... [8,27-29] Since WPRO, including China, has recently initiated a program to eliminate measles in 2012, maybe a variety of genotypes will be detected in China as the intensity of the measles control ... the predominant cluster circulating in China in 2004 There were multiple lineages in each cluster These data reinforce the observation that multiple chains of transmission were present in areas...
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Báo cáo hóa học: " Involvement of intracellular free Ca2+ in enhanced release of herpes simplex virus by hydrogen peroxide" ppt
... role of polymorphonuclear leukocyte infiltration in herpes simplex virus infection of murine skin Arch Dermatol Res 1999, 291:28-36 Milligan GN: Neutrophils aid in protection of the vaginal mucosae ... 6C), indicating the induction of DNA fragmentation When FI cells were infected with HSV-1 at an MOI of PFU/cell and cultured for 18 h, the profile of DNA content was different from that of mock-infected ... staining and annexin V staining Thus, the H2O2-induced cell death occurred in this situation was not apoptosis The apoptosis of HSV-1-infected cells by H2O2 may be prevented the function of anti-apoptotic...
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báo cáo hóa học:" Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide" pdf
... recombinant nectin-1 was shown to block entry of virus through vaginal mucosa [21], suggesting the importance of nectin-1 in mediating entry of HSV into the female genital tract Hve-A and nectin bind ... re-amplification of hybridoma cDNAs, elimination of degenerate primer sequences introduced in the first reaction and introduction of 2/3 of a 15 amino acid hinge region comprising three repeats of four glycine ... addition of Larginine and oxidized glutathione (GSSG) in final two steps [28] The ability of the single chain antibodies produced in this way to bind their target antigen was tested by determining...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... secretion of IFN-γ at sites of HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans ... behavior of HSV-1 in NOD scid mice as the basis for concluding that NK cells play no role in innate resistance to HSV-1 Likewise, we question the validity of comparisons of HSV-1 infection in mice ... replication of herpesviruses [59-62] In the case of HSV-1, IFN-γ achieves this effect by multiplying the potency with which IFN-α/β inhibits viral replication [28] The uncontrolled spread of HSV-1 in...
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Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea" docx
... they may in fact consist of a mixture of two different kinds of viruses Distinguishing between these possibilities will require further characterization using cloned viruses The evolution of IBV ... [15,20] Widespread uses of various vaccines made from heterologous IBVs in the field may also exert pressure resulting in the increase of new genetic variants in Korea In summary, 12 Korean field ... cells carrying recombinant plasmids were selected on Luria-Bertani agar plates containing ampicillin, X-gal and IPTG, and plasmid DNA for sequencing was prepared using the E.Z.N.A plasmid miniprep...
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Báo cáo y học: " Presence of Human Herpes Virus 6 (HHV6) in pediatric lymphomas: impact on clinical course and association with cytomegalovirus infection" docx
... members of the herpesvirus family including (CMV), Epstein-Barr virus, varicella zoster virus and herpes simplex viruses and PCRs were performed in a final volume of 20 μl, which included μl of DNA ... staging, and end results of treatment of childhood non - hodgkins lymphoma: dissimilarities from lymphoma in adults Semin Oncol 1980, 7:332-339 15 Link MP, Eeinslein HJ: Principles and practice of ... and those negative for both viruses The presence of herpes viruses (HHV6 and/or CMV) in lymphoma patients Herpes viruses (HHV6 and/or CMV) were present in 23 of the 50 in lymphoma patients (46%),...
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Báo cáo y học: " Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data" doc
... usefulness of assessing genital HSV infection by single qPCR measurement of HSV DNA in cervicovaginal lavage (CVL) specimens of women with mostly longstanding HSV-2 infection within the setting of a ... Some of the challenges in ascertaining these issues are related to intermittent nature and wide variability in the frequency and amount of HSV shedding observed among infected individuals Many investigators ... Nevertheless, findings of this study were the basis for classifying HSV-2 infected women into groups of gradient degree of GH clinical activity (determined by the presence/absence of active symptomatic...
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