... Oxidative half -reaction of wild-type and variants of DAAO.
(A) Comparison of time courses of the (re)oxidation of reduced
wild-type and W50 variants of DAAO followed at 455 nm upon mix-
ing of 10 ... contributes to the d-amino acid preference of
yeast DAAO.
Kinetic mechanism of DAAO variants
Steady-state kinetics
Dependence of the catalytic activity of DAAO variants
on the oxygen and d -alanine concentrations ... half -reaction of wild-type and variants of DAAO.
Comparison of time courses of flavin reduction followed at 455 nm
(vertical bars = experimental data points) for W50 variants (A) and
T201L ⁄ V and...
... consistency and texture of fermented food. One hundred and fifteen
strains of Lactic Acid Bacteria (LAB) were isolated and characterized from some fermented dairy (“Nono”,
“Fura”, Yogurt, “Wara”) and ... their biosynthesis and molecular organization and
mainly because of their GRAS (generally regarded as safe) fermentation strategy is rather scarce and the kinetics of
status [1] and their rheological ... 10ml of mESM broth and
microbiological analysis. the broths were incubated anaerobically for 24h at 30°C.
Isolation of lactic acid bacteria: Serial dilutions of flasks containing 90 ml of mESM...
... Dental Sciences and Surgery, University of Bari, Bari, Italy.
Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of
Bari, P.zza ... Larsson A, Welander U,
Frithiof L, Engstrom GN. The effect of hyaluronan on bone and
soft tissue and immune response in wound healing. J Perio-
dontol. 2001 Sep; 72(9):1192-200.
Tables and Figures ... and other tissues. HA
structure consists of polyanionic disaccharide units of
glucouronic acidand N-acetyl-glucosamine con-
nected by alternating β 1–3 and β1–4 bonds [1]. There
is no anti-genic...
... findings of none
detectable. The authors also reported 101 ( 38 and 22.1 ( 15.2
àg -carotene/g FW in extracted samples of aril and mesocarp,
respectively, and 16 and 9 àg/g of zeaxanthin and 35 and ... identification of major carotenoids, includes carotenoid
profiles, measuring both trans- and cis-isomers of lycopene and
β-carotene. We also provide a detailed fatty acid analysis of
gac seed and aril, ... linoleic and R-linoleic acids.
The aril also contains a significant, but varying, amount of
stearic acidand small amounts of cis-vaccenic, myristic, eicosa-
11-enoic, arachidic, and palmitoleic acids.
Our...
... substrate specificity of
Table 2. Kinetic parameters for the reductive and oxidative half-reactions of wild-type, H181A, H184A and Y186F PETN reductases. Kinetic
data are shown in Figs 3 and 4. Parameters ... architec-
ture of OYE1 and PETN reductase, and (ii) geometry
for the binding of steroid compounds and 2-cyclohe-
xen-1-one [2,19]. The structure of the Y186F enzyme is
essentially identical to that of wild-type ... 2005)
doi:10.1111/j.1742-4658.2005.04875.x
The roles of His181, His184 and Tyr186 in PETN reductase have been
examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by
determination of crystallographic structures...
... length and orientation of helices. The superposition of
the IPDC monomer on the subunit of ScPDC and ZmPDC
results in rmsd of 1.24 A
˚
for 470 out of 563 Ca atoms
and 1.48 A
˚
for 496 out of 568 ... from
L
-trypto-
phan, and consists of three steps: (a) the conversion of
tryptophan to indole-3-pyruvic acid; (b) the formation of
indole-3-acetaldehyde; and (c) the production of IAA
(Fig. 1). The first step of ... structure elements of IPDC are shown in
Fig. 4, together with the aligned amino acid sequences of
IPDC and ZmPDC. The topology of the IPDC monomer is
similar to that of ScPDC and ZmPDC with some...
... producing
transformant and was used for the purification of GALA.
Growth of this transformant and subsequent purification of
the enzyme were performed as described in Materials and
methods. Purity of the preparation ... Purification and properties of a-
L
-arabinofuranosidase
and endo-b-D-1,4-galactanase from Aspergillus niger v. Tieghem
KF-267 which liquified the Okara. In Genetics, Biochemistry and
Ecology of Cellulose ... to facilitate purification
of GALA. The enzyme has a molecular mass of 48.5 kDa
and a pH optimum between 4 and 4.5. Incubations of ara-
binogalactans of potato, onion and soy with GALA resulted
initially...
... the
basis of the kinetic constants in Table 1, and those obtained
for the glutaminase reaction in the presence of 0.1 m
M
each
of UTP and ATP-cS (data not shown). Apparently, the
value of K
a
, ... of nucleotides (Table 1).
The rate of CTP synthesis was dramatically influenced by
the presence of GTP, and 20 and 41-fold increases in k
cat
were obtained with GTP concentrations of 0.1 m
M
and
1m
M
, ... activation of the
glutaminase activity in the presence of 0.1 m
M
(open circles) or 1 m
M
(closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTP
synthesis at 0.1 m
M
each of UTP and ATP.
4776...
... results of a steady-state kinetic
analysis of DptE. The aim of this kinetic study was to
determine the specificity of DptE for various fatty
acids and noncognate ACPs. The Michaelis–Menten
kinetic ... chain fatty acids and
Table 2. Kinetic parameters for steady-state analysis of the DptE-
catalysed lipidation of DptF determined at varying concentrations of
fatty acids or DptF, LipD and AcpK.
Substrate ... fatty
acids naturally found in A21987C. Hexanoic acid,
palmitic acidand 15-methylhexadecanoic acid were not
accepted by DptE.
In summary, DptE is capable of transferring a
variety of fatty acids...
... protection of enzymes and membranes from the
detergent effects of excess fatty acids by sequestering
them, and (d) modulation of cell growth and differenti-
ation by transport of fatty acids to the nucleus ... uptake of fatty
acids across the plasma membrane and transport to
various subcellular organelles, (b) modulation of the
activity of enzymes involved in fatty acid metabolism,
(c) protection of enzymes ... consists of four exons of
112, 174, 89 and 141 bp, coding for 22, 59, 30 and 20
amino acids, respectively. The intron ⁄ exon structure of
the fabp6 gene from zebrafish is consistent with that of
all...