rankl a weak activator of nf κb in macrophages does not protect from mtx induced apoptosis
Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt
... application of OP-1 protein as therapeutic treatment for cartilage regeneration and repair Abbreviations ADAM: a disintegrin and metalloproteinase; ALK: activin-like kinase; AP-1: activator protein-1; ... 5’-AACAGAAGCGGTTGATGATGGTGC-3’ Forward 5’-GTCAATTCGTTCTGAAGAGGTGAGT-3’ 64°C, 40 sec [GenBank:NM_000600] Reverse 5’-CCCCAGGAGAAGATTCCAAAGATG-3’ IL-8 Forward 5’-AGACATACTCCAAACCTTTCCACCC-3’ 58°C, 30 sec [GenBank:NM_000584] ... [GenBank::NM_001200] [GenBank:NM_002046] Reverse 5’-CGGGAAGCTTGTCATCAATGGAA-3’ Gremlin-1 Forward 5’-ATACCTGAAGCGAGACTGGTGCAA-3’ 64°C, 40 sec [GenBank:NM_013372] Reverse 5’-AACAGAAGCGGTTGATGATGGTGC-3’...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... TCCATCTGGCTCCTGCTGAAGAAC GCTTCTGAGGCACTCAGGCTGAAC 19 20 STMN1 TGM2 21-25 21-36 AAAGAACTGGAGAAGCGTGCCTCAG CTGGTCACTAACCAACAAGGTTG CTGAATTTCCTCCAGGGAAAGATCC GAGCAGGAGATAAAGTCAAAGCTG Ct: cycle threshold Watanabe ... GTACGGGATCAAATGCGCCAAG CATCTCCAATACGCATTCGTCCATC AGGCCACACAGCGGAAACACTC 13 MALL(BENE) 21-33 CATTACATCCGTGGATTCTCC GTGCTTAAGAGAATGTGAGGG 14 PLAU 21-31 CGCTGCTCCCACATTGGCTAAG TGTGCATGGGTGAAGGGAGAGC 15 RAB25 ... TGATCGGCGAATCAGGTGTGG CAACATCACAGTGCGGGTGGAG 16 S10 0A2 22-27 GCAGCCTGGATGAGAACAGTGACC CAGCCCTGGAAGAAGTCATTGCAC 17 S100P 18-32 GTCTGAATCTAGCACCATGACG GGAAGCCTGGTAGCTCCTTC 18 SLCO 4A1 22-32 TCCATCTGGCTCCTGCTGAAGAAC...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... cttgtacatatgagcgagaaaagcgaagaa cgttctcgagaatgaaaacattgattc gcgcggatccttaacttgtatataaata cgcgctcgagctacttttcatataaata SEE M21319 ggtagccatatgagcgaagaaataaatgaa gcgcggatcctcaagttgtgtataaata SEG AF064773 ... gcgcggatcctcaactttcgtccttata SElN AF285760 aatgctcatatggacaaaaaagatttaaag gcgcggatccttaatctttatataaaa SElO AF285760 tgcactcgagaatgaagaagatcctaaa cgcgctcgagttatgtaaataaataaac Seo et al Journal of Translational Medicine ... tgtgcatatgcaacccgatcctaaatta gcgcggatcctcagtgagtattaaga SEI AF285760 tgctctcgaggatattggtgtaggtaac cgcgctcgagttagttactatctacata SElM AF285760 cgcacatatggatgtcggagttttgaat gcgcggatcctcaactttcgtccttata...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... types infected, MOI, time of infection and percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage ... Genomics 2001, 7(2):97-104 Warrington JA, Nair A, Mahadevappa M, Tsyganskaya M: Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes Physiol Genomics ... the assay to detect changes in the expression of genes of interest and may also produce artificial changes [3] Traditionally glyceraldehyde 3phosphate dehydrogenase (GAPDH) and β-actin (BACT) have...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... genes remained constant, while other genes were varying in expression according to accumulation of infected cells The experimentally obtained data for each virus and each gene were analysed using ... position, indicating that it is an unsuitable reference gene in virus infected cells The actin gene shows significant variations with increasing degree of infection The best genes obtained from all ... gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... types infected, MOI, time of infection and percentage of cells infected are shown in table Cells were infected with each virus strain at a MOI and duration in order to achieve the maximal percentage ... Genomics 2001, 7(2):97-104 Warrington JA, Nair A, Mahadevappa M, Tsyganskaya M: Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes Physiol Genomics ... the assay to detect changes in the expression of genes of interest and may also produce artificial changes [3] Traditionally glyceraldehyde 3phosphate dehydrogenase (GAPDH) and β-actin (BACT) have...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... genes remained constant, while other genes were varying in expression according to accumulation of infected cells The experimentally obtained data for each virus and each gene were analysed using ... position, indicating that it is an unsuitable reference gene in virus infected cells The actin gene shows significant variations with increasing degree of infection The best genes obtained from all ... gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... 5'GGGCATTTTGGGTTATGT TG3' 5'TCCCCACTCGTTGTCATA CC3' 146 bp 5'CAGCAAGGGAGGAACCAG TA3' 5'TAGCGCAAGACCATCAAC AA3' 130 bp 5'ATGAAGGCGATGAAGGAG AA3' 5'GTACGCAATGGAATGGAA CC3' 112 bp 5'GGTCTTGCTCTCCATCTG CT3' ... 5'CGGGCTGTCGTCTCATAC TT3' 114 bp 5'ACCAGCACAAATCAAAGG A3 ' 5'GCCAAAGTATGAGACGAC AGC3' 149 bp 5'GTCACTAAGCCATCGGTC GT3' 5'ACACGGACACAACCAGTT CA3' 112 bp 0.94 0.020 GE617482 *Amplification efficiency was calculated ... procedure was crucial to guaranteeing the same amount of starting material and equivalent efficiency of cDNA synthesis from total RNA samples Based on DNA analysis by agarose gel electrophoresis and...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... CCTGGTCAAATTGGAAACGG/ CAGATCGCCTGTCAATCTTGG 103 1.62 ± 0.08 AACAACTCACTCCTACACCGG/ GGTAGCACTAGAGACACAGCCTT CAGGCAGGTTAAGGCAAAGC/ CTAGCAAGGTACAGAAACGGC AAGCTCCCACCTGTCTGGAAA/ AACAGATTGCCGGAAGCCA CTTACGACGAGTTCAGATGCC/ ... an optimal normalization factor that merges data from all of them Finally, NormFinder fits data to a mathematical model, which allows comparison of intra- and intergroup variation and calculation ... stage analysed (RAN1, SAND and UBQ) Stability of gene expression in Mitchell and V30 Data from each of the two chosen Petunia lines were analyzed separately As a first approach, we applied data...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... 307:12-21 13 Kirisawa R, Hosoi Y, Yamaya R, Taniyama H, Okamoto M, Tsunoda N, Hagiwara K, Iwai H: Isolation of equine herpesvirus-1 lacking glycoprotein C from a dead neonatal foal in Japan Arch Virol ... related AHV-1 and gC DNA vaccine research Methods Viruses and bacteria AHV-1 Cha strain and Escherichia coli JM109 were obtained from Key Laboratory of Animal Diseases and Human Health of Sichuan ... copies of most tissues (other than kidney) reached a peak at hours postinoculation in AHV-1 Cha strain-infected ducks The concentration of nucleic acid in DNA vaccine-inoculated ducks maintained...
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Báo cáo y học: "Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients" pps
... et al Critical Care 2011, 15:412 http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics ... 133 130,000 Discharged from ICU 105 137,000 Died in ICU ICU stay (days) Viremia peak is expressed in copies/ml DBSA, deep burn surface area; TBSA, total burn surface area ... characteristics Patient Age (years) TBSA (%) 80 76 a DBSA (%) IGS score 15 41 40 40 38 82 15 15 54 60 28 20 29 Viremia peaka Outcome 30 9,130 Discharged from ICU 141 63,400 Died in ICU 133 130,000 Discharged...
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Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
... quantitative assay of ultra low concentration of pDNA in biological materials Such data are necessary for the assessment of the risk of residual plasmid presence in consumable parts of DNA vaccinated ... Genetic Vaccines and Therapy 2008, 6:11 Background DNA-based vaccines represent a new and rapidly progressing area in vaccinology So far, plasmid DNA (pDNA) vaccines have been reported to induce protective ... support Special thanks to Hana Kudláèková for assistance with animal handling and sampling, and to Jana Plocková for manuscript preparation References Alarcon JB, Waine GW, McManus DP: Adv Parasitol...
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Quantitative real time PCR
... Osteopontin TTAAACAGGCTGATTCTGGAAGTTC 60 GATTCTGCTTCTGAGATGGGTCA 99 105 221 NM_000389 p21 CTGGAGACTCTCAGGGTCGAA GGATTAGGGCTTCCTCTTGGA 60 99 98 523 NM_004360 E-cadherin TCCTCAGAGTCAGACAAAGACCAG TCCTCGCCGCCTCCGTAC ... Lin School of Medicine, National University of Singapore, Singapore, Singapore ROBERTO BIASSONI • Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Istituto Giannina Gaslini, ... incorrect data analysis were leading to the routine publication of data that were often inconsistent, inaccurate, and wrong The problem was exacerbated by a lack of transparency of reporting, with...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... serving as a measurement of cellularity, amplification of GAPDH can also be used as an indicator of adequate RNA integrity in samples A range of acceptable GAPDH C(t) values is selected to insure ... virtually all of the genes relevant to inflammatory synovitis [25] This approach also eliminates template source-based variation in PCR amplification, a significant cause of assay nonlinearity ... technical assistance and Dr Tanya Wolfson and Dr Karin Ernstrom for statistical analysis This work was supported, in part, by a grant from Pharmacia, Inc GSF and DLB have served as consultants...
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Luận văn :Định lượng kháng nguyên Cyfra 21-1 bằng kỹ thuật Real-time PCR
... L E F V A E I T K D G GGTGGCACATGGTACGGGTCGGCGGTGAAGGGCCGTGCCACCATCTCGAGGGACAACGGG 540 G G T W Y G S A V K G R A T I S R D N G CAGAGCACAGTGAGGCTGCAGCTGAACAACCTCAGGGCTGAGGACACCGGCATCTACTGC 600 ... GGTGTCAGCGAACCCGGGAGAACCGTCGAGATCACCTGCTCCGGGGGTGGTAGCAGCAAC 60 G V S E P G R T V E I T C S G G G S S N TACTATGGCTGGTACCAGCAGAAGGCACCTGGCAGTGCCCCTGTCAGTCTGATCTATGCT 120 Y Y G W Y Q Q K A P G S A P V S L I Y A AACACCAACAGACCCTCAAACATCCCTTCACGATTCTCCGGTTCTGGATCCGGCTCCACA ... AACACCAACAGACCCTCAAACATCCCTTCACGATTCTCCGGTTCTGGATCCGGCTCCACA 180 N T N R P S N I P S R F S G S G S G S T AACACATTAACCATCACTGGGGTCCAAGCCGACGACGAGGCTGTCTATTTCTGTGGGAGT 240 N T L T I T G V Q A D D E A V...
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Kỹ thuật Real time PCR chẩn đóan sớm HIV trên trẻ em
... biến vùng quê Campuchia 13 Agence Nationale de recherches sur le Sida et les hépatites virales Cám ơn quan tâm quí vị 14 Agence Nationale de recherches sur le Sida et les hépatites virales ... Faso, Central African Republic • Đông Nam Á: Việt Nam Cambodia (bắt đầu từ tháng 1-7/2005) • Đánh giá Campuchia •9/2005: Kiểm định ANRS •11/2005: Kiểm định NIH • Ứng dụng Campuchia •từ 3/2005: ... phức tạp, nhiều thời gian -Cần labo an tòan mức độ 3 Agence Nationale de recherches sur le Sida et les hépatites virales Mục tiêu Xây dựng Chuẩn h a Đánh giá Kỹ thuật real time PCR Để chẩn đoán...
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Phát hiện và định lượng virus gây bệnh đốm trắng trên tôm sú bằng kỹ thuật Real - time PCR
... Decapoda - Bộ mười chân Họ chung: Penaeidae Họ: Penaeidae - Họ tơm he Giống: Penaeus Lồi: Penaeus monodon Tên đ a phương: tơm sú, tơm cỏ, tơm he, tơm giang (Trần Minh Anh, 1989) Tiếng Anh: Black ... chu kỳ ngưỡng SQ: starting quantity: số ban dầu SD: standard deviation: độ lệch chuẩn Mean: trung bình Kết kiểm tra mẫu qua Real - time PCR phân tích chủ yếu d a trên: số ban đầu (SQ), giá trị ... sung với hai mạch khn, sau bắt đầu tổng hợp ADN in vitro nhờ ADN polymerase chịu nhiệt Sau giai đoạn primer bắt cặp, ADN polymerase kéo dài primer, tạo trình tự mạch khn nằm hai primer Sau kéo dài...
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Xây dựng quy trình định lượng các sản phẩm biến đổi gen bằng phương pháp Real-time PCR
... hybridization probe Taqman Quantification ability: Detection and quantification copy of p35S in a experiment template, exactly quantification a template which percentage ratio of GMO presentation has ... Organisms) QUANTIFICATION BY REAL-TIME PCR METHOD Instructors: Le Dinh Don and Nguyen Thai Thuy Recently, GMO is a controversial problem in the world Because its advantages and disadvantages cannot ... quantification ability and efficiency of method Applying it for quantification some of food and feed derived from maize in Ho Chi Minh City Results: A process of GM maize quantification by Real-Time...
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XÂY DỰNG QUY TRÌNH PHÁT HIỆN ĐỘT BIẾN rtA181V/T VÀ rtN236T KHÁNG ADEFOVIR CỦA VIRUS VIÊM GAN B (Hepatitis B Virus) BẰNG KỸ THUẬT REAL-TIME PCR
... CACTGAAATGGCACTAGTAAACTGAGT** 699 - 670 P181 FAM-AGAAACGGACTGAGGCCCACTCCCA-TAMRA 641 – 665 F236 TGGCTTTCAGTTATATGGATGATGTGGT 724 - 751 R236 CCCCAWCKTTTKGTTTTRTKAGGGG*** 680 - 836 P236 FAM-TGGGGGCCAAGTCTGTACAACATCTTGAGT-TAMRA ... (Hoa Kỳ) Bảng 2.1: Trình tự vị trí mồi probe sử dụng đề tài Tên Trình tự 5’→3’ Vị trí nt F181 TATTCCCATCCCATCATCYTGGGCT 601 - 625 R181-1 CACTGAAATGGCACTAGTAAACTGAA* 699 - 671 R181-2 CACTGAAATGGCACTAGTAAACTGAGT** ... nhân điều trị sau năm [26,29,39] Asparagine (N) thay Threonine (T) codon 236 vùng RT (rtN236T) alanine (A) thay Valine (V) Threonine (T) codon 181 vùng RT (rtA181V/T) nhận biết kháng ADV [38] Trên...
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XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG CYTOMEGALOVIRUS (CMV) TRONG MÁU, NƯỚC TIỂU BẰNG PHƯƠNG PHÁP REAL-TIME PCR
... 3’ATCCACATCTCCCGCTTATCCTCAGGTACAATGTAGTTCTCATACATGCTCTGCATAGT TAGCCCAATACACTTCATCTCCTCGAAAGGCTCATGAAC5’- vị trí 1071-gen UL123–CMV 3.1.1.2 Trình tự nucleotide hệ primers TaqMan probe D a phần mềm ClustalX tương đồng gen UL ... Primer ngược ® TaqMan probe 5’-TCCTCGAAAGGCTCATGAAC-3’ 5’TCTCATACATGCTCTGCATAGTTAGCC CAA3’ 1052- 1071 1011- 1040 Kết chạy ClustalX hệ primers –TaqMan probe v a thiết kế Với primer xuôi: Hình ... bệnh HHV-6 não ghép (Transplantencephalopathy), bệnh xơ cứng rải rác Gammaherpesvirinae HHV-7 Bệnh ban đào (roseola) Kaposi sarcoma, lymphoma HHV-8 đáy xoang thể, bệnh sarcoid 1.2 Tình hình nhiễm...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008