range cell tropism and evolution studies of canine and feline parvoviruses minute virus of mice porcine parvovirus and aleutian mink disease virus
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... spectra of selectively 15N-Ala labelled w in complex with v and c, where w, v and c are subunits of the E coli DNA polymerase III complex (A) w was produced by cellfree synthesis in the presence of ... observation and dynamics of specific amide protons in T4 lysozyme Biochemistry 24, 817–822 McIntosh LP & Dahlquist FW (1990) Biosynthetic incorporation of 15N and 13C for assignment and interpretation of...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1 ... encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments...
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THE DYNAMICAL PROCESSES OF BIODIVERSITY – CASE STUDIES OF EVOLUTION AND SPATIAL potx
Ngày tải lên :
28/06/2014, 08:20
... processes involved in the evolution and diversification The Dynamical Processes of Biodiversity Case Studies of Evolution and Spatial Distribution of the Vanilla genus and concluded that Vanilla ... gradient of species richness illustrates the complex evolutionary history of global biodiversity, and many studies have placed it in the context of geological history and rates of speciation and extinction ... occurrence of 2n egg cells was not investigated, but ploidy analysis of the progeny proved that 2n egg cells 38 The Dynamical Processes of Biodiversity Case Studies of Evolution and Spatial...
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from dust to stars studies of the formation and early evolution of stars
Ngày tải lên :
03/07/2014, 21:09
... From Dust to Stars Studies of the Formation and Early Evolution of Stars Norbert S Schulz From Dust to Stars Studies of the Formation and Early Evolution of Stars Published in association ... exemplary missions, SOFIA as the successor of the airborne Kuiper Observatory of the 1980s and early 1990s, JWST as the successor of Hubble, and the space probes Herschel and GAIA SOFIA and Herschel ... The study of the formation and early evolution of stars has been an ever growing part of astrophysical research Traditionally, often in the shadow of its big brothers (i.e., the study of stellar...
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Báo cáo y học: "The promise and limitations of population exomics for human evolution studies" doc
Ngày tải lên :
09/08/2014, 23:20
... of chromosomes Figure The influence of sample size in detecting recent population expansions (a) Demographic model used in the simulations Times of demographic events (in units of thousands of ... statistical tests of selective neutrality, but the overlap among these lists of candidate regions is often poor, suggesting a high proportion of false positives [4] In addition, it is often unclear ... genetics and phenotype association studies In association analyses, the goal is usually to maximize the number of putatively real variants, any of which could be causal for the trait in question, and...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
Ngày tải lên :
12/08/2014, 04:21
... a virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... 2.5 × 105 cells and bound virus was analysed with RT-PCR B) Decreasing number of CHO and HeLa cells, 2.5 × 105-2.5 × 102, were incubated with MOI 0.05 TCID50 /cell of CVB5 and attached virus was ... valuable technology for studies of interactions between virus and cells even when low amounts of cells or viruses are available Investigating viral binding using labeled and purified viruses is usually...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
Ngày tải lên :
13/08/2014, 22:22
... along with a great deal of skill and hard work In one set of experiments on single cells [17], they made a measurement of the dry mass of single cells by interferometry and then placed it in the ... rate of cell growth and rate of DNA synthesis in Escherichia coli B/r Proc Natl Acad Sci USA 1971, 68:1061-1064 Prescott DM: Relations between cell growth and cell division Reduced weight, cell ... Determination of DNA, RNA and mass content of age determined mouse fibroblasts in vitro and of intercellular variation in generation time Exp Cell Res 1965, 38:272-284 Zetterberg A, Killander D: Quntitative...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
Ngày tải lên :
02/11/2012, 11:08
... state of CNS dis-inhibition involved in the pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes and and ... relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness on ... levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] The decreases in P3 and novelty P3 with increasing age, and indeed...
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Control of Cell Proliferation and Growth byMyc Proteins
Ngày tải lên :
25/10/2013, 21:20
... deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the ... of proteins (see Fig 1) may dictate the response to loss of Myc (Walker et al 2005) In mice, the effects of deletion of the c-myc or n-myc genes in vivo are not uniform Deletion of c-myc in mice ... in cell proliferation: for example, deletion of n-myc in neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... characterization of numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided into two domains: a core domain and ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... Escherichia coli cells In the structure the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
Ngày tải lên :
16/02/2014, 09:20
... components of the reductive TCA cycle and catalyze the anabolic reductive carboxylation of acetyl-CoA and succinyl-CoA, respectively [17,18] Four of the five subunits correspond to those of the heterotetrameric ... reduction of ferredoxin Thus, the identification and characterization of enzymes that transfer electrons to ferredoxins in vivo is of particular importance for the improvement of this coupled assay and ... reaction and vice versa Thus, further studies on the POR reactions in both directions would lead to a deeper understanding of the overall reaction mechanism of this enzyme Materials and methods...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
Ngày tải lên :
16/02/2014, 09:20
... A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of His34 ... we report the results of the structural studies of the pantoate-bound form of the N-terminal domain of E coli PS (nPS) The structure and substrate-binding interactions of E coli nPS have been ... specific residues of nPS as a function of substrate(s) The resonances of free nPS are shown in red, those of nPS bound to D-pantoate are shown in blue, and those of nPS bound to D-pantoate and ATP are...
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Tài liệu A REVIEW OF INDOOR AIR POLLUTION AND HEALTH PROBLEMS FROM THE VIEWPOINT OF ENVIRONMENTAL HYGIENE: FOCUSING ON THE STUDIES OF INDOOR AIR ENVIRONMENT IN JAPAN COMPARED TO THOSE OF FOREIGN COUNTRIES pptx
Ngày tải lên :
17/02/2014, 22:20
... the Ministry of Health, Labour, and Welfare of Japan announced the guideline value of formaldehyde first and individual volatile organic compounds (VOCs).13, 14) In Japan, occupants of newly built ... conducted to confirm and extend the U.S.A case definition of MCS The results showed a significant overlap of MCS, chronic fatigue syndrome (CFS) and fibromyalgie, and that no standard diagnostic procedure ... consideration of public health.38) The relationship between moisture and temperature on skin and upper airway symptoms was investigated and showed that skin dryness and rashes, pharyngeal dryness, and...
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
Ngày tải lên :
18/02/2014, 18:20
... Chang cells On the basis of the different usages of exon and exon 1a in Huh7 and Chang cells, we decided to analyze the relationship between usage of exon 1a and P1 promoter methylation In liver cell ... Chang cells, and the PCR product was sequenced (D) PCRs for exon and exon 1a were performed in Huh7 and Chang cells (E) The boundary regions of exon and exon in the genomic DNA of Chang cells ... and OccDE9-overexpressing cells (Fig 6A) Specifically, in Chang and Huh7 cells, OccWT significantly decreased the expression of MMP2, MMP7, MMP9 and MMP14, and of MMP1, MMP2, MMP3, MMP7, MMP9 and...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
Ngày tải lên :
19/02/2014, 00:20
... 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ protons of DHNP and DHMP are shown The chemical structures of ... those of the reduced pterin HP (0.47 lm)1Æs)1 and 13 s)1, respectively), suggesting that HPO is an excellent analogue for HP and, by analogy, NP and MP are excellent analogues of DHNP and DHMP ... NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional to the different values of...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... Kirschbaum B & Loffler M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified ... value for dThd and dCyd, of approximately 200- and 100-fold, respectively The kcat values were only decreased approximately twofold for both substrates Phosphorylation of nucleosides and nucleoside ... R-factor and Rfree were 23.2 and 24.2%, respectively (Table 3) Most of the protein could be found in the electron-density map, with the exception of residues 1–11 and 210–230 The loop connecting a9 and...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
Ngày tải lên :
19/02/2014, 05:20
... the salt optima of the enzymes are fine-tuned to match the salinity of their environment The outer membrane and cell wall of Gram-negative bacteria not restrict passage of ions, and the periplasmic ... characterization of the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum NaCl concentrations for activity were found to be 425 mm for VsEndA and ... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and...
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Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
Ngày tải lên :
20/02/2014, 01:20
... A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) High molecular weight bands in lanes and correspond to mAb A10.2- and mAb ... cytospun cells (F) Cells incubated with Glu-Pg and 0.5 lM of mAb 34D3 No cells were recovered after cytospining the supernatant of control and Glu-Pg ⁄ mAb 34D3-treated cells *P < 0.0001 vs control cell- bound ... control cells (B, C) Cells incubated with Glu-Pg: (B) residual adherent cells; (C) detached cytospun cells (D, E) Cells incubated with Glu-Pg and 0.5 lM of mAb A10.2: (D) residual adherent cells;...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
Ngày tải lên :
20/02/2014, 23:20
... change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... change in Trp85 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... spectra of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor at the excitation wavelength QRF is the quantum yield of the reference...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL [21,34] support ... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... (black) and two distinct structures of the lid (orange) Ó FEBS 2002 Dimeric model structure and function of lipoprotein lipase (Eur J Biochem 269) 4705 Table Heparin affinity of C-terminal domain of...
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