quantitation of virus infectivity
Báo cáo sinh học: " Peptide inhibitors of dengue virus and West Nile virus infectivity" pot
... [8,9] Additional peptide mimics of the fusion proteins of other retroviruses, and of orthomyxoviruses, paramyxoviruses, filoviruses, coronaviruses, and herpesviruses have also been identified ... in the trimeric structures Several disparate viruses, including arenaviruses, coronaviruses, filoviruses, orthomyxoviruses, paramyxoviruses and retroviruses, encode similar proteins that together ... primary sequence of E1, the fusion protein of Semliki Forest virus, an alphavirus, revealed a remarkable fit to the scaffold of TBEV E [20] suggesting the existence of a second class of viral fusion...
- 10
- 392
- 0
báo cáo hóa học:" Peptide inhibitors of dengue virus and West Nile virus infectivity" doc
... [8,9] Additional peptide mimics of the fusion proteins of other retroviruses, and of orthomyxoviruses, paramyxoviruses, filoviruses, coronaviruses, and herpesviruses have also been identified ... in the trimeric structures Several disparate viruses, including arenaviruses, coronaviruses, filoviruses, orthomyxoviruses, paramyxoviruses and retroviruses, encode similar proteins that together ... primary sequence of E1, the fusion protein of Semliki Forest virus, an alphavirus, revealed a remarkable fit to the scaffold of TBEV E [20] suggesting the existence of a second class of viral fusion...
- 10
- 363
- 0
Báo cáo y học: "BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo" pot
... volume of 20 μl of × buffer for rTaq DNA Polymerase (TOYOBO) containing 250 nM of 256 primer and 453 primer (see below), 0.5 units of rTaq polymerase, 0.2 mM of dNTPs, 2.5 mM of MgCl2, and μl of ... LTRs of other retroviruses Evaluation of the sensitivity of BLV-CoCoMo-qPCR compared with nested PCR To determine the sensitivity of BLV-CoCoMo-qPCR, 20 solutions, each containing 0.7 copies of ... HTLV-1, human immunodeficiency virus type (HIV-1), simian immunodeficiency virus (SIV), mouse mammary tumor virus (MMTV), Molony murine leukemia virus (M-MLV), and a range of plasmids including pUC18,...
- 19
- 229
- 0
Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... using deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand ... involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA Results Effect of mutations in the 3¢- or the 5¢-end of ... alter the structure of the other domains of the (–)IRES RNA as determined by predicted secondary structure with RNA Draw software The structure of the first 151 nt nucleotides of wild-type and mutated...
- 15
- 597
- 0
Báo cáo sinh học: " Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp" potx
... Rohwer F, Chisholm SW: Transfer of photosynthesis genes to and from Prochlorococcus viruses Proceedings of the National Academy of Sciences of the United States of America 2004, 101:11013-11018 ... Sequences of probes for the amplification of signal RNA, capture and detection of SMART signal, and of synthetic product for ELOSA standard curve have all been published previously [1,4] Page of (page ... cyanophage S-PM2 Proceedings of the National Academy of Sciences of the United States of America 2001, 98:11411-11416 Chen F, Lu JR: Genomic sequence and evolution of marine cyanophage P60: a...
- 8
- 411
- 0
Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... concentration of 0.5% Serial two-fold dilutions of virus suspensions were prepared in Alsever's buffer and 50 µL of viral suspension and an equal volume of RBC were added into each well of a 96-well ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of...
- 5
- 358
- 0
báo cáo hóa học:" SPECT/CT-plethysmography – non-invasive quantitation of bone and soft tissue blood flow" ppt
... scan using an in-house software that performs segmentation of the bone and soft tissue for each CT slice, subsequently creating corresponding regions of interest The regions of interest are copied ... height of the virtual cylinder on which measurements were performed was of approximately 1.4 – 2.1 cm Precise calculation of the entire cylinder volume (VL) is provided by the CT component of the ... limb (green) Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2008, 3:36 The limb (and thus the)virtual cylinder is composed of soft tissue (mainly...
- 8
- 406
- 0
Báo cáo hóa học: " Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp" doc
... Rohwer F, Chisholm SW: Transfer of photosynthesis genes to and from Prochlorococcus viruses Proceedings of the National Academy of Sciences of the United States of America 2004, 101:11013-11018 ... Sequences of probes for the amplification of signal RNA, capture and detection of SMART signal, and of synthetic product for ELOSA standard curve have all been published previously [1,4] Page of (page ... cyanophage S-PM2 Proceedings of the National Academy of Sciences of the United States of America 2001, 98:11411-11416 Chen F, Lu JR: Genomic sequence and evolution of marine cyanophage P60: a...
- 8
- 422
- 0
Báo cáo hóa học: " La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys" docx
... cells that were infected Viruses such as vesicular stomatitis virus, rabies virus, mouse hepatitis virus, Borna disease virus, pseudorabies virus, and herpes simplex virus have all been demonstrated ... Figuredistribution of La Crosse virus following intraperitoneal (IP) inoculation of Swiss Webster mice with 104.5 PFU Tissue Tissue distribution of La Crosse virus following intraperitoneal (IP) inoculation of ... Figuredistribution of La Crosse virus following intraperitoneal (IP) inoculation of Swiss Webster mice with 102.5 PFU Tissue Tissue distribution of La Crosse virus following intraperitoneal (IP) inoculation of...
- 15
- 411
- 0
Báo cáo hóa học: " The temperature arrested intermediate of virus-cell fusion is a functional step in HIV infection" ppt
... fusion The averages of triplicate experiments are shown (n = 4) Error bars represent the standard error of the mean The concentrations of inhibitors were: uM of AMD 3100 and ug/mL of PRO 542 if present ... would take time, resulting in the delay of fusion of virus- cell cultures maintained at TAS relative to control cells Therefore, the kinetic predisposition of virus- cell cultures to advance beyond ... example, for the entry of rous sarcoma virus (RSV), lipid mixing is pHindependent, while the completion of the fusion process is pH-dependent [8] Further, the formation of a fusion pore appears...
- 7
- 334
- 0
báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... concentration of 0.5% Serial two-fold dilutions of virus suspensions were prepared in Alsever's buffer and 50 µL of viral suspension and an equal volume of RBC were added into each well of a 96-well ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of...
- 5
- 327
- 0
báo cáo hóa học:" Quantitation of glucose uptake in tumors by dynamic FDG-PET has less glucose bias and lower variability when adjusted for partial saturation of glucose transport" potx
... illustrates the distribution of COV values for each of the three PET metrics Sensitivity analysis for the between-animal COV as a function of KM Varying the value of KM in the range of 40 to 200 mg/dL ... instrument in 20 mice and showed a coefficient of variation of 3.7% Image analysis Regions of interest [ROIs] were drawn using the image analysis software IRW from Siemens For any given tumor model, ... and strength of this relationship between blood glucose and tissue uptake rates when seen in the metaanalysis of our large sample of studies Figure illustrates this using a box plot of Pearson's...
- 31
- 470
- 0
Báo cáo y học: "Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon a" pptx
... Program of the People’s Republic of China (NO 200801020 and NO 200902174) and also supported by a grant from Ministry of Education of China (NO 200901121) We thank Wensheng Luo, university of Maryland ... methods, DNA primers and probes[20] Relative amount of MxA mRNA level were reported as number of folds of MxA mRNA copies relative to the expression of GAPDH Increasing folds= mean relative amount ... group)] Real-time analysis of MxA mRNA and HBV DNA levels Determination of hepatitis B surface antigen (HBsAg) For real-time analysis of MxA mRNA detection, 500 ng of total RNA were converted...
- 6
- 351
- 0
Báo cáo y học: " Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli" pptx
... frame of porcine circovirus type encodes a major capsid protein J Gen Virol 2000, 81:2281-2287 Fan H, Ju C, Tong T, Huang H, Lv J, Chen H: Immunogenicity of empty capsids of porcine circovirus ... The reaction of Cap-tag and nCap may with anti-PCV2 pig sera and was observed by Western blotting (Fig 3) Cleavage of His-Smt3 tag and assembly of Cap protein VLPs After the cleavage of the His-Smt3 ... Only the deletion of the whole NLS domain may allow for the expression of a large amount of the Cap protein However, the NLS plays an important role forming the self assembly of Cap-like particles...
- 5
- 454
- 0
Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot
... contained a mixture of × 105 copies of HPV6 L1, × 104 copies of HPV11 L1, × 103 copies of HPV16 L1 and × 102 copies of HPV18 L1 The assay was performed in the presence or absence of 100 ng of DG-75 DNA ... detection of any of these types The second qPCR assay targets the coding region of the hypervariable loop V of the L1 gene of HPV6, 11, 16 and 18 These HPV types are associated with the majority of ... http://www.virologyj.com/content/7/1/194 the quantitation of viral DNA to address the role of viral load in disease progression Real time quantitative PCR (qPCR) allows for quantitation of DNA over orders of magnitude [11]...
- 17
- 413
- 0
Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... MA, Maniloff J, Desselberger U, Bell LA: Virus Taxonomy Elsevier Academic Press; 2005 Pallanch MA, Roos RP: Enteroviruses: polioviruses, coxsackieviruses, ehcoviruses and newer enteroviruses fourth ... of interactions between virus and cells even when low amounts of cells or viruses are available Investigating viral binding using labeled and purified viruses is usually conducted with high virus ... counting B) MOI 0.05 TCID50/cell of the labeled, purified virus and C) MOI 0.05 TCID50/cell of unpurified virus incubated as described in A) and the amount of virus bound to the cell surface was...
- 6
- 230
- 0
Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
... India Methods Viruses Sixteen strains of dengue viruses, including five strains of DENV-1, four strains of DENV-2, three strains of DENV-3, four strains of DENV-4 and one strain each of JE, WN and ... (Fig 2B) For quantitation by PFU, ten fold dilution of RNA extracted from virus stocks of all four serotypes of DENV with known PFU titers were used The PFU titers of the stock viruses ranged ... [(number of positive specimens/(number of positive specimens + number of false negative specimens)] The specificity of the assay was evaluated using RNA extracted from virus stocks of two related...
- 8
- 352
- 0
Báo cáo y học: " Modification of a loop sequence between -helices 6 and 7 of virus capsid (CA) protein in a human " pot
... Saturation of intrinsic antiviral factors resulting from inoculation of high dose of virus particles Saturation of intrinsic antiviral factors resulting from inoculation of high dose of virus particles ... The amount of RT per ng of CA of NL-ScaVR (0.083 ng) was comparable to that of NLScaVR6/7S (0.081 ng), indicating that the replacement of L6/7 in HIV-1 with the corresponding loop of SIVmac did ... density of × 104 cells per well in a 24-well plate Prior to GFP virus http://www.retrovirology.com/content/6/1/70 infection, the cells were pretreated for hours with 200 ng of p24 of each of the...
- 11
- 235
- 0
Báo cáo khoa học: "Epidemiology and clinical outcome of virus-positive respiratory samples in ventilated patients: a prospective cohort study" pptx
... influenza virus B and influenza virus C, respiratory syncytial virus, metapneumovirus, rhinovirus, coronavirus 229E and coronavirus OC43, adenovirus, cytomegalovirus and herpes simplex virus Chlamydia ... and virus parainfluenza 3, one case of rhinovirus and cytomegalovirus, one case of herpes simplex virus type and virus influenza A, and one case of herpes Page of 10 (page number not for citation ... rhinovirus and virus influenza were the most frequently recovered viruses These epidemiological data underscore the potential pathogenic role of rhinovirus and of influenza virus as the cause of...
- 10
- 194
- 0
Báo cáo y học: "Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo" doc
... monitoring of virus spread, HLH cultures were employed to additionally analyze the effects of Nef on depletion of CD4+ T lymphocytes and revealed a significant contribution of Nef to both virus replication ... http://www.retrovirology.com/content/6/1/6 increase of virus replication is provided by Nef in cultures of isolated PBLs [27-30] and Nef also augments virus replication in cocultures of antigen presenting cells and ... optimal HIV-1 replication Of note, comparison of p24 production between wt and Δnef HIV-1 in HLAC revealed comparable growth of both viruses in approximately one half of the experiments, while...
- 14
- 264
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25